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Applied Microbiology and Biotechnology Jan 2024Honeybee (Apis mellifera) is an important agricultural pollinator and a model for sociality. In this study, a deep knowledge on yeast community characterizing the...
Honeybee (Apis mellifera) is an important agricultural pollinator and a model for sociality. In this study, a deep knowledge on yeast community characterizing the honeybees' environmental was carried out. For this, a total of 93 samples were collected: flowers as food sources, bee gut mycobiota, and bee products (bee pollen, bee bread, propolis), and processed using culture-dependent techniques and a molecular approach for identification. The occurrence of yeast populations was quantitatively similar among flowers, bee gut mycobiota, and bee products. Overall, 27 genera and 51 species were identified. Basidiomycetes genera were predominant in the flowers while the yeast genera detected in all environments were Aureobasidium, Filobasidium, Meyerozyma, and Metschnikowia. Fermenting species belonging to the genera Debaryomyces, Saccharomyces, Starmerella, Pichia, and Lachancea occurred mainly in the gut, while most of the identified species of bee products were not found in the gut mycobiota. Five yeast species, Meyerozyma guilliermondii, Debaryomyces hansenii, Hanseniaspora uvarum, Hanseniaspora guilliermondii, and Starmerella roseus, were present in both summer and winter, thus indicating them as stable components of bee mycobiota. These findings can help understand the yeast community as a component of the bee gut microbiota and its relationship with related environments, since mycobiota characterization was still less unexplored. In addition, the gut microbiota, affecting the nutrition, endocrine signaling, immune function, and pathogen resistance of honeybees, represents a useful tool for its health evaluation and could be a possible source of functional yeasts. KEY POINTS: • The stable yeast populations are represented by M. guilliermondii, D. hansenii, H. uvarum, H. guilliermondii, and S. roseus. • A. pullulans was the most abondance yeast detective in the flowers and honeybee guts. • Aureobasidium, Meyerozyma, Pichia, and Hanseniaspora are the main genera resident in gut tract.
Topics: Bees; Animals; Yeasts; Ascomycota; Pichia; Gastrointestinal Microbiome; Flowers
PubMed: 38276993
DOI: 10.1007/s00253-023-12942-1 -
Foods (Basel, Switzerland) Nov 2023This study identified and tested fruit-isolated yeasts against three major postharvest citrus pathogens, namely, , , and , and further evaluated the impact of FeCl on...
This study identified and tested fruit-isolated yeasts against three major postharvest citrus pathogens, namely, , , and , and further evaluated the impact of FeCl on the biocontrol efficiency of pulcherrimin-producing strains. Based on the characterization of the pigmented halo surrounding the colonies and the analysis of the D1/D2 domain of 26S rDNA, a total of 46 sp. were screened and identified. All 46 strains significantly inhibited the hyphal growth of , , and , and effectively controlled the development of green mold, blue mold and sour rot of citrus fruit. The introduction of exogenous FeCl at certain concentrations did not significantly impact the pulcherriminic acid (PA) production of pigmented strains, but notably diminished the size of pigmented zones and the biocontrol efficacy against the three pathogens. Iron deficiency sensitivity experiments revealed that and exhibited higher sensitivity compared to , indicating that iron dependence varied among the three pathogens. These results suggested that strains, capable of producing high yields of PA, possessed great potential for use as biocontrol agents against postharvest citrus diseases. The biocontrol efficacy of these yeasts is mainly attributed to their ability to competitively deplete iron ions in a shared environment, with the magnitude of their pigmented halo directly correlating to their antagonistic capability. It is worth noting that the level of sensitivity of pathogens to iron deficiency might also affect the biocontrol effect of pulcherrimin-producing .
PubMed: 38231683
DOI: 10.3390/foods12234249 -
Zootaxa Jan 2024The online World Porifera Database (WPD), the Porifera part of the World Register of Marine Species (WoRMS), lists virtually all published scientific names of sponges....
The online World Porifera Database (WPD), the Porifera part of the World Register of Marine Species (WoRMS), lists virtually all published scientific names of sponges. The names of the WPD (as indeed all names in WoRMS) are guided by the Code of the International Comnission on Zoological Nomenclature (ICZN). The WPD names include all currently accepted as well as original combinations, and a majority of non-accepted non-original combinations. Currently, among the accepted names about 200 original lower taxa combinations were found to be accepted by default, usually varieties or formae, which were given that status in the WPD because there was no sufficient published information to support arguments for or against the accepted status. After 1961, varieties and formae are considered infrasubspecific taxa whose names are not regulated by the Code and the names are unavailable, but prior to that date these trinominal taxa are potential available names. It is the purpose of the present study to evaluate these original default accepted combinations and arrive at an argumented judgement on whether they are to be truly accepted or non-accepted. Furthermore, additional lower taxa name violations of the Code are also included. Overall, there are three categories of names of lower taxa treated here, (1) with combinations, which are judged to be accepted with elevated status as (sub)specific taxa, (2) with combinations judged to be junior synonyms, and (3) with combinations, which are violating articles of the Code. Among the last category there are a small number of varietal taxa described after the 1960 cut-off date, which are unavailable but are proposed to have the original name combination retained, but as new names with authorship and year changed to those of the present article. Also, names found to be unavailable for various reasons (four-name combinations, junior homonyms, phylocode names) are in this category. The following 31 new names are proposed (three of which are junior synonyms of senior accepted names, and an additional three are unavailable names made available by employing the same name combination with the present authorship and year): Aaptos hoshinoi nom.nov., Ancorina nanosclera nom.nov., Axinella kurushima nom.nov., Callyspongia (Cladochalina) desqueyrouxfaundezae nom.nov., Cliona carpenteri subsp. hentscheli nom.nov., Callyspongia (Toxochalina) gustavoi nom.nov., Craniella microspira nom.nov., Dictyaulus romani nom.nov., Grantia breitfussi nom.nov., Haliclona alba subsp. albapontica nom.nov., Haliclona aquaeductus subsp. sebastopolensis nom.nov., Haliclona inflata subsp. vladimiri nom.nov., Haliclona informis subsp. voldomaroi nom.nov., Haliclona palmata subsp. pontuseuxiniensis nom.nov., Haliclona (Gellius) arthuri nom.nov., Haliclona (Gellius) godthaabae nom.nov., Haliclona merguiensis nom.nov., Haliclona senjitanitai nom.nov., Iophon hentscheli nom.nov., Leucandra wilsoni nom.nov., Paraleucilla bassensis nom.nov. (= P. saccharata), Pione carpenteri subsp. hentscheli nom.nov., Psammocinia samaaii nom.nov., Protoschmidtia czerniavskyi nom.nov. (= Metschnikowia tuberculata), Reiswiginella nom.nov., Scalarispongia lamarcki nom.nov., Spheciospongia hentscheli nom.nov., Spongia (Spongia) vonlendenfeldi nom.nov. (= S. (S.) lignosa), Suberites austral nom.nov., Suberites dendyi nom.nov., Suberites simae nom.nov., and Timea levii nom.nov.
Topics: Animals; Names; Authorship; Databases, Factual; Fireflies; Porifera
PubMed: 38221180
DOI: 10.11646/zootaxa.5398.1.1 -
Fish & Shellfish Immunology Feb 2024DM9-containing protein in invertebrates functions as pattern recognition receptor (PRR) to play significant roles in innate immunity. In the present study, a novel...
DM9-containing protein in invertebrates functions as pattern recognition receptor (PRR) to play significant roles in innate immunity. In the present study, a novel DM9-containg protein (defined as EsDM9CP-1) was identified from the Chinese mitten crab Eriocheir sinensis. EsDM9CP-1 is composed of 330 amino acids containing a Methyltransf_FA domain and two tandem DM9 repeats. The deduced amino acid sequence of EsDM9CP-1 shared low similarity with the previously identified DM9CPs from other species, and it was closely clustered with Platyhelminthes DM9CPs and then assigned into the branch of invertebrate DM9CPs in the unrooted phylogenetic tree. The mRNA transcripts of EsDM9CP-1 were highly expressed in haemocytes, gill, and heart. After Aeromonas hydrophila stimulation, the expression levels of EsDM9CP-1 mRNA in haemocytes increased significantly at 3 h (3.88-fold, p < 0.05) and 6 h (2.71-fold, p < 0.05), compared with that of PBS group, respectively. EsDM9CP-1 protein was mainly distributed in the cytoplasm and membrane of haemocytes. The recombinant EsDM9CP-1 protein (rEsDM9CP-1) exhibited binding affinity to MAN, PGN, LPS and Poly (I:C), and also to Gram-positive bacteria (Staphylococcus aureus, Micrococcus luteus and Bacillus subtilis), Gram-negative bacteria (Escherichia coli, A. hydrophila and Vibrio splendidus) and fungi (Pichia pastoris and Metschnikowia bicuspidata) in a Ca-dependent manner. It was able to agglutinate A. hydrophila, S. aureus, M. luteus, M. bicuspidata and P. pastoris, and inhibit the growth of A. hydrophila and M. bicuspidate. These results suggested that EsDM9CP-1 in crab not only functioned as a PRR, but also agglutinated and inhibited the growth of microbes.
Topics: Humans; Animals; Phylogeny; Staphylococcus aureus; Base Sequence; Receptors, Pattern Recognition; Immunity, Innate; RNA, Messenger; Brachyura; Hemocytes
PubMed: 38163495
DOI: 10.1016/j.fsi.2023.109356 -
International Journal of Food... Feb 2024The maintenance of the balance between oxidised and reduced redox cofactors is essential for the functioning of many cellular processes in all living organisms. While...
The maintenance of the balance between oxidised and reduced redox cofactors is essential for the functioning of many cellular processes in all living organisms. While the electron transport chain plays a key role in maintaining this balance under respiratory conditions, its inactivity in the absence of oxygen poses a challenge that yeasts such as Saccharomyces cerevisiae overcome through the production of various metabolic end-products during alcoholic fermentation. In this study, we investigated the diversity occurring between wine yeast species in their management of redox balance and its consequences on the fermentation performances and the formation of metabolites. To this aim, we quantified the changes in NAD(H) and NADP(H) concentrations and redox status throughout the fermentation of 6 wine yeast species. While the availability of NADP and NADPH remained balanced and stable throughout the process for all the strains, important differences between species were observed in the dynamics of NAD and NADH intracellular pools. A comparative analysis of these data with the fermentation capacity and metabolic profiles of the strains revealed that Saccharomyces cerevisiae, Torulaspora delbrueckii and Lachancea thermotolerans strains were able to reoxidise NADH to NAD throughout the fermentation, mainly by the formation of glycerol. These species exhibited good fermentation capacities. Conversely, Starmerella bacillaris and Metschnikowia pulcherrima species were unable to regenerate NAD as early as one third of sugars were consumed, explaining at least in part their poor growth and fermentation performances. The Kluyveromyces marxianus strain exhibited a specific behaviour, by maintaining similar levels of NAD and NADH throughout the process. This balance between oxidised and reduced redox cofactors ensured the consumption of a large part of sugars by this species, despite a low fermentation rate. In addition, the dynamics of redox cofactors affected the production of by-products by the various strains either directly or indirectly, through the formation of precursors. Major examples are the increased formation of glycerol by S. bacillaris and M. pulcherrima strains, as a way of trying to reoxidise NADH, and the greater capacity to produce acetate and derived metabolites of yeasts capable of maintaining their redox balance. Overall, this study provided new insight into the contribution of the management of redox status to the orientation of yeast metabolism during fermentation. This information should be taken into account when developing strategies for more efficient and effective fermentation.
Topics: Saccharomyces cerevisiae; Wine; NAD; Glycerol; Fermentation; NADP; Phylogeny; Oxidation-Reduction; Sugars
PubMed: 38150773
DOI: 10.1016/j.ijfoodmicro.2023.110537 -
FEMS Yeast Research Jan 2024Pulcherrimin is an iron (III) chelate of pulcherriminic acid that plays a role in antagonistic microbial interactions, iron metabolism, and stress responses. Some...
Pulcherrimin is an iron (III) chelate of pulcherriminic acid that plays a role in antagonistic microbial interactions, iron metabolism, and stress responses. Some bacteria and yeasts produce pulcherriminic acid, but so far, pulcherrimin could not be produced in Saccharomyces cerevisiae. Here, multiple integrations of the Metschnikowia pulcherrima PUL1 and PUL2 genes in the S. cerevisiae genome resulted in red colonies, which indicated pulcherrimin formation. The coloration correlated positively and significantly with the number of PUL1 and PUL2 genes. The presence of pulcherriminic acid was confirmed by mass spectrometry. In vitro competition assays with the plant pathogenic fungus Botrytis caroliana revealed inhibitory activity on conidiation by an engineered, strong pulcherrimin-producing S. cerevisiae strain. We demonstrate that the PUL1 and PUL2 genes from M. pulcherrima, in multiple copies, are sufficient to transfer pulcherrimin production to S. cerevisiae and represent the starting point for engineering and optimizing this biosynthetic pathway in the future.
Topics: Saccharomyces cerevisiae; Botrytis; Metschnikowia; Iron
PubMed: 38140959
DOI: 10.1093/femsyr/foad053 -
Life (Basel, Switzerland) Dec 2023Consumers today seek safe functional foods with proven health-promoting properties. Current evidence shows that a healthy diet can effectively alleviate oxidative stress...
Consumers today seek safe functional foods with proven health-promoting properties. Current evidence shows that a healthy diet can effectively alleviate oxidative stress levels and reduce inflammatory markers, thereby preventing the occurrence of many types of cancer, hypertension, and cardiovascular and neurological pathologies. Nevertheless, as fruits and vegetables are mainly consumed fresh, they can serve as vectors for the transmission of pathogenic microorganisms associated with various disease outbreaks. As a result, there has been a surge in interest in the microbiome of fruits and vegetables. Therefore, given the growing interest in sweet cherries, and since their microbial communities have been largely ignored, the primary purpose of this study is to investigate their culturome at various maturity stages for the first time. A total of 55 microorganisms were isolated from sweet cherry fruit, comprising 23 bacteria and 32 fungi species. Subsequently, the selected isolates were molecularly identified by amplifying the 16S rRNA gene and ITS region. Furthermore, it was observed that the communities became more diverse as the fruit matured. The most abundant taxa included and among the bacteria, and , , and among the fungi.
PubMed: 38137924
DOI: 10.3390/life13122323 -
Foods (Basel, Switzerland) Dec 2023Interest in () is growing in the world of winemaking. is used both to protect musts from microbial spoilage and to modulate the aromatic profile of wines. Here, we...
Interest in () is growing in the world of winemaking. is used both to protect musts from microbial spoilage and to modulate the aromatic profile of wines. Here, we describe the isolation, characterization, and use of an autochthonous strain of in the vinification of Chasselas musts from the 2022 vintage. was used in co-fermentation with at both laboratory and experimental cellar scales. Our results showed that does not ferment sugars but has high metabolic activity, as detected by flow cytometry. Furthermore, sensory analysis showed that contributed slightly to the aromatic profile when compared to the control vinifications. The overall results suggest that our bioprospecting strategy can guide the selection of microorganisms that can be effectively used in the winemaking process.
PubMed: 38137289
DOI: 10.3390/foods12244485 -
Anais Da Academia Brasileira de Ciencias 2023Harsh and extreme environments, such as Antarctica, offer unique opportunities to explore new microbial taxa and biomolecules. Given the limited knowledge on microbial...
Harsh and extreme environments, such as Antarctica, offer unique opportunities to explore new microbial taxa and biomolecules. Given the limited knowledge on microbial diversity, this study aimed to compile, analyze and compare a subset of the biobank of Antarctic fungi maintained at the UNESP's Central of Microbial Resources (CRM-UNESP). A total of 711 isolates (240 yeasts and 471 filamentous fungi) from marine and terrestrial samples collected at King George Island (South Shetland Islands, Antarctica) were used with the primary objective of investigating their presence in both marine and terrestrial environments. Among the yeasts, 13 genera were found, predominantly belonging to the phylum Basidiomycota. Among the filamentous fungi, 34 genera were represented, predominantly from the phylum Ascomycota. The most abundant genera in the marine samples were Metschnikowia, Mrakia, and Pseudogymnoascus, while in the terrestrial samples, they were Pseudogymnoascus, Leucosporidium, and Mortierella. Most of the genera and species of the CRM-UNESP biobank of Antarctic fungi are being reported as an important target for biotechnological applications. This study showed the relevance of the CRM-UNESP biobank, highlighting the importance of applying standard methods for the preservation of the biological material and associated data (BMaD), as recommended in national and international standards.
Topics: Antarctic Regions; Biological Specimen Banks; Fungi; Yeasts; Ascomycota; Basidiomycota
PubMed: 38126380
DOI: 10.1590/0001-3765202320230603 -
Oecologia Feb 2024The dilution effect hypothesis, which suggests greater host biodiversity can reduce infectious disease transmission, occurs in many systems but is not universal. Most...
The dilution effect hypothesis, which suggests greater host biodiversity can reduce infectious disease transmission, occurs in many systems but is not universal. Most studies only investigate the dilution of a single parasite in a community, but many host communities have multiple parasites circulating. We studied a zooplankton host community with prior support for a dilution effect in laboratory- and field-based studies of a fungal parasite, Metschnikowia bicuspidata. We used paired experiments and field studies to ask whether dilution also occurred for a bacterial parasite, Pasteuria ramosa. We hypothesized that the similarities between the parasites might mean the dilution pattern seen in Metschnikowia would also be seen in Pasteuria. However, because Daphnia-Pasteuria interactions have strong host-parasite genotype specificity, dilution may be less likely if diluter host genotypes vary in their capacity to dilute Pasteuria. In a lab experiment, Pasteuria prevalence in susceptible Daphnia dentifera was reduced strongly by higher densities of D. pulicaria and marginally by higher densities of D. retrocurva. In a second experiment, different D. pulicaria genotypes had a similar capacity to dilute both Metschnikowia and Pasteuria, suggesting that Pasteuria's strong host-parasite genotype specificity should not prevent dilution. However, we found no evidence of an impact of the dilution effect on the size of Pasteuria epidemics in D. dentifera in Midwestern U.S. lakes. Our finding that a second parasite infecting the same host community does not show a similar dilution effect in the field suggests the impact of biodiversity can differ even among parasites in the same host community.
Topics: Animals; Bacteria; Biodiversity; Daphnia; Genotype; Lakes
PubMed: 38105355
DOI: 10.1007/s00442-023-05486-8