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Analytica Chimica Acta Jul 2024Mineral oil aromatic hydrocarbon (MOAH) analysis in foods is a major analytical challenge. Quantification is associated with a high uncertainty. The sources of...
BACKGROUND
Mineral oil aromatic hydrocarbon (MOAH) analysis in foods is a major analytical challenge. Quantification is associated with a high uncertainty. The sources of uncertainty are multiple, but the major one is related to data interpretation and integration, which is partially derived from insufficiently efficient sample preparation. Recently, an updated ISO method for the analysis of mineral oil in fats and oils and a standard operating procedure for infant formula analysis have been published. Both methods reported significantly different (up to 1.25) distributions of the internal standards used for quantification (i.e., tri-tert-butyl benzene (TBB) and 2-methyl naphthalene (2-MN)) over the different solvent phases used in the saponification step.
RESULTS
In this work, a microwave-assisted saponification and extraction method was optimized for MOAH analysis to solve the problem related to the MOAH internal standards partition. The paper examines the impact of the solvent mixture used, the concentration of KOH on the partition of TBB and 2-MN, and the effect of the matrix and the washing step to extract the unsaponifiable fraction containing the mineral oils.
SIGNIFICANCE
The optimized procedure achieved a TBB/2-MN ratio of 1.05 ± 0.01 tested in five different fats and oils, namely, sunflower, rapeseed, coconut, palm, and extra virgin olive oils. The method can significantly contribute to reducing the uncertainty of the MOAH quantification when saponification is applied.
Topics: Microwaves; Hydrocarbons, Aromatic; Mineral Oil
PubMed: 38834273
DOI: 10.1016/j.aca.2024.342788 -
Archives of Razi Institute Dec 2023Foot-and-mouth disease (FMD) has a high prevalence in cloven-hoofed animals. It is also highly contagious and remains a serious threat to livestock worldwide. Despite... (Comparative Study)
Comparative Study
Foot-and-mouth disease (FMD) has a high prevalence in cloven-hoofed animals. It is also highly contagious and remains a serious threat to livestock worldwide. Despite the widespread vaccination program in Iran, outbreaks of FMD continue to occur. Vaccination is one of the most effective methods of preventing FMD. The vaccines used in Iran are of the inactivated type and contain several serotypes. Since inactivated vaccines without adjuvants do not induce a high and durable antibody response, it is necessary to use adjuvants. Montanide ISA 206 VG is a mineral oil-based adjuvant that produces a water-in-oil-in-water (w:o:w) emulsion in vaccine preparations. However, a large number of manufacturers in Iran and around the world still use alum adjuvant (with or without saponin) to produce the FMD vaccine. This study used Montanide ISA 206 and alum adjuvants to administer the O2010 serotype of the FMD virus to goats. A total of six goats were divided randomly into three groups. Vaccines were administered subcutaneously twice, at a one-month interval. Blood sampling was done at different times, and the micro-neutralization method was used to measure the neutralizing antibody titer in each serum. Seven days after the second vaccination, the alum group's antibody titer was higher but not statistically significant. However, from the 28th day after the second injection until the end of the study, the Montanide ISA 206 group's antibody titer was significantly higher than that of the alum group. Six months after the second injection, the antibody titer in the ISA 206 group remained at the peak level, while in the alum group, it decreased and reached the minimum protective level. Nine months after the second injection, the antibody titer remained at its peak level in the ISA 206 group, whereas it dropped significantly in the alum group. Based on the findings, ISA 206 VG is capable of generating long-term humoral immunity in goats against the FMD serotype O2010 and could replace aluminum hydroxide adjuvants in FMD vaccine preparations.
Topics: Animals; Goats; Aluminum Hydroxide; Foot-and-Mouth Disease Virus; Goat Diseases; Foot-and-Mouth Disease; Adjuvants, Immunologic; Viral Vaccines; Antibodies, Neutralizing; Antibodies, Viral; Iran; Oleic Acids; Mannitol
PubMed: 38828163
DOI: 10.32592/ARI.2023.78.6.1843 -
ACS Omega May 2024The exploration potential of shale oil in the Fengcheng Formation of the Mahu Sag, located in the Junggar Basin, is significant. However, there is a notable dearth of...
The exploration potential of shale oil in the Fengcheng Formation of the Mahu Sag, located in the Junggar Basin, is significant. However, there is a notable dearth of research on shale oil within this formation. This study addresses this gap by focusing on the pore structure and associated development factors of the Fengcheng Formation in Mahu Sag. A total of 113 samples from well X of the Fengcheng Formation were meticulously selected for analysis. The mineral components and pore structure of these samples were characterized using advanced techniques, including X-ray diffraction (XRD), low-temperature nitrogen adsorption (LTNA), high-pressure mercury injection (HPMI), and scanning electron microscopy (SEM). The findings reveal that, despite a relatively low content of clay minerals in the Fengcheng Formation, their presence is intricately linked to pore development. The high content of feldspar in the formation contributes significantly to the formation of clay minerals through dissolution processes. This dual influence plays a crucial role in shaping the overall pore development within the Fengcheng Formation. In addition, a comparative analysis was conducted with shale samples from other wells within the Fengcheng Formation in Mahu Sag, as well as from different basins, such as the Songliao Basin and Ordos Basin. Different from Qingshankou Formation in Songliao Basin and Yanchang Formation in Ordos Basin, the samples from Fengcheng Formation in Mahu Sag are composed of a large number of felic minerals and carbonate minerals with less clay minerals. This study underscores the paramount importance of mineral components and their respective content in influencing the pore development of shale oil reservoirs. The unique characteristics of the Fengcheng Formation in Mahu Sag, as revealed through comprehensive analyses, contribute valuable insights to the understanding of shale oil exploration potential in the Junggar Basin.
PubMed: 38826555
DOI: 10.1021/acsomega.4c02056 -
ACS Omega May 2024Although abundant unconventional oil resources have been discovered in conglomerate and sandstone reservoirs in rift basins, the mechanism of differential pore evolution...
Although abundant unconventional oil resources have been discovered in conglomerate and sandstone reservoirs in rift basins, the mechanism of differential pore evolution in conglomerates and sandstone reservoirs within different secondary structural zones of rift basins is not yet clear. The pore structures of conglomerate and sandstone reservoirs in the distinct secondary structural zones in the Chezhen Sag were quantified in three dimensions using high-resolution microcomputed tomography (micro-CT). Thin section and scanning electron microscopy observations were used to investigate the differential evolution mechanisms of conglomerate and sandstone reservoirs. Micro-CT analysis of the pore structures of conglomerate and sandstone reservoirs revealed that sandstone reservoirs are superior to conglomerate reservoirs with regard to the pore number and pore connectivity and that sandstone reservoirs are more heterogeneous than conglomerate reservoirs. Triangles dominate the pore and pore throat geometries of sandstone and conglomerate reservoirs, while the sandstone reservoir pores are more regular than conglomerate reservoir pores. The depositional environment, mineral composition, and diagenetic intensity jointly control the quality of the reservoirs. Because of the lengthy transportation distance of their parent rocks, the compositional maturity and sorting behavior of sandstone reservoirs in depression and gentle slope zones are better than those of conglomerate reservoirs in steep slope zones, and thus sandstone reservoirs have a higher initial porosity than conglomerate reservoirs. The rapid compaction experienced by the conglomerate reservoirs in steep slope zones in their early stages creates a closed diagenetic environment, making it difficult to effectively improve reservoir porosity through dissolution. However, the widely developed microfractures in the reservoirs provide channels for fluid migration, promote the development of dissolution pores, and form a tight reservoir dominated by secondary pores. With weak compaction and an open diagenetic environment, the primary pores in sandstone reservoirs in the gentle slope zone are preserved in large quantities. Meanwhile, dissolution expands the secondary pores of the reservoir, resulting in a high-quality reservoir having both primary and secondary pores. In addition, an approach based on primary, secondary, and total porosity was proposed in the study to efficiently evaluate reservoir quality and identify reservoir evolution mechanisms.
PubMed: 38826526
DOI: 10.1021/acsomega.4c02106 -
Scientific Reports May 2024It is very important to clarify the reservoir damage characteristics and damage characteristics and damage mechanism of tight sandstone gas reservoirs in Jinhua-Zhongtai...
It is very important to clarify the reservoir damage characteristics and damage characteristics and damage mechanism of tight sandstone gas reservoirs in Jinhua-Zhongtai mountain area of central SiChuan Basin, and put forward the technical countermeasures of "Double protection" drilling fluid to protect the reservoir and the environment, which is very important for the efficient well construction in this area. Through X-ray diffraction, scanning electron microscopy, casting thin sections and other testing methods, the mineral composition and microstructure characteristics of the block were analyzed, and the potential damage factors of the reservoir were clarified. Based on the sensitivity evaluation, it was revealed that the overall sensitivity damage of the block was weak. The main damage type was salt-sensitive damage, and the critical salinity was 9472.5 mg/L. On the basis of the environmental protection drilling fluid system used in this block, the surfactant which can effectively prevent gas invasion and reduce surface tension is selected, and the "Double protection" drilling fluid system is constructed. Through comprehensive performance test and reservoir protection performance evaluation, the core permeability damage rate of the optimized drilling fluid system is reduced from 88.77 to 18.66%, and the cuttings recovery rate is increased to more than 66%, and the cuttings expansion rate is reduced to less than 3.2%, which can effectively solve the problem of reservoir damage in drilling in Jinhua-Zhongtai mountain block in central Sichuan.
PubMed: 38816516
DOI: 10.1038/s41598-024-62696-7 -
Food Additives & Contaminants. Part A,... Jul 2024A low-cost and effective method is reported to identify water and synthetic milk adulteration of cow's milk using coffee ring patterns. The cow's milk samples were...
A low-cost and effective method is reported to identify water and synthetic milk adulteration of cow's milk using coffee ring patterns. The cow's milk samples were diluted with tap water (TW), distilled water (DW) and mineral water (MW) and drop cast onto glass slides to observe coffee ring patterns. The area of the ring, total particle area and average particle diameter were extracted from these patterns. For each ring, the ratio of total particle area versus total ring area was calculated. The area ratio, regardless of water adulterants, follows an exponential model with respect to average particle diameter. Unlike TW, the ratio for DW and MW adulterated milk are clustered and classified together with respect to the particle diameter. These results were independent of dilution level and are used for adulterant classification. The ring of milk adulterated using synthetic milk gave multiple concentric rings, flower-like structures, and oil globules throughout the dilution level. An Alexnet model was used to classify water and synthetic milk adulterants in authentic milk. The trained model could achieve 96.7% and 95.8% accuracy for binary and tertiary classification respectively. These results enable us to distinguish synthetic milk from pure milk and segregate DW and MW with respect to TW adulterated milk.
Topics: Milk; Animals; Food Contamination; Neural Networks, Computer; Cattle; Water
PubMed: 38814700
DOI: 10.1080/19440049.2024.2358518 -
Reproductive Biology May 2024This study investigates the influence of four culture media pre-equilibration methods on embryo development and clinical pregnancy outcomes. The methods are as follows:...
This study investigates the influence of four culture media pre-equilibration methods on embryo development and clinical pregnancy outcomes. The methods are as follows: Method A involved covering media with fresh mineral oil in humid-type incubators for 24 h. Method B replicated Method A in dry-type incubators. Method C utilized pre-equilibrated (humidified) mineral oil to cover the media, also in humid-type incubators for 24 h. Method D followed the same process as Method C but in dry-type incubators. Subsequently, media from all groups were transferred to dry-type incubators for 72 h. Osmolality was measured at 24, 48, 72, and 96 h. For G1 PLUS, no significant differences were observed among groups at 24, 48, and 72 h. However, at 96 h, Groups B and D exhibited significantly higher osmolality than Groups A and C (A vs B, p = 0.043; A vs D: p = 0.046; B vs C, p = 0.043; C vs D, p = 0.046). No significant variations were found between Groups A and C or B and D. Similar results were obtained for G2 PLUS. A retrospective analysis of embryo development and clinical outcomes using Methods A revealed significant improvements in good blastocysts and available embryos compared with Method B for all (p = 0.005 and 0.004) and IVF cycles (p = 0.025 and 0.017). Method A also enhanced blastocyst formation in ICSI cycles (p = 0.017). However, clinical pregnancy outcomes did not significantly differ between Methods A and B. Pre-equilibrating culture media overnight in humid-type incubators, even when covered with fresh mineral oil, significantly mitigates osmolality rise and improves embryo development potential during embryo culture in dry-type incubators.
PubMed: 38810436
DOI: 10.1016/j.repbio.2024.100897 -
Nanotechnology Jun 2024Artificially synthesized DNA is involved in the construction of a library of oil tracers due to their unlimited number and no-biological toxicity. The strategy of the...
Artificially synthesized DNA is involved in the construction of a library of oil tracers due to their unlimited number and no-biological toxicity. The strategy of the construction is proposed by oleophilic Silica-encapsulated DNA nanoparticles, which offers fresh thinking in developing novel tracers, sensors, and molecular machines in engineering & applied sciences based on artificially synthesized DNA blocks.
Topics: Silicon Dioxide; DNA; Nanoparticles; Oils
PubMed: 38806006
DOI: 10.1088/1361-6528/ad50db -
The Science of the Total Environment Aug 2024Pore type and pore structure evolves systematically across continuous black shale weathering profile. However, the extend and process of pore structure change is still...
Pore type and pore structure evolves systematically across continuous black shale weathering profile. However, the extend and process of pore structure change is still an enigma. In this study, we try to unveil the pore structure evolution during weathering process through studying Cambrian Hetang shales in southern China. Fourteen shale samples, from protolith zone (PZ), fractured and weathered shale zone (FWZ), and saprolite zone (SZ), were collected to elucidate how porosity and pore structure develop during black shale weathering under subtropical condition. Through low pressure argon (Ar) gas adsorption (LP-ArGA), high pressure mercury intrusion (HPMI), nuclear magnetic resonance(NMR) and field emission scanning electron microscope (FESEM) observation, the results reveal significant differences in physical properties and pore structures among the PZ, FWZ, and SZ samples. Specifically, compared to PZ, FWZ and SZ samples are characterized by higher clay mineral content, lower organic matter (OM), and the absence of carbonates and pyrite. Total porosity, determined through HPMI and NMR, exhibits a gradual increase from PZ (6.70 % and 6.41 %) to FWZ (20.47 % and 13.45 %) and SZ (23.22 % and 12.48 %). Ar adsorption isotherms indicate a change in pore type from predominantly ink-bottle and slit-shaped in the PZ to mainly slit-shaped in FWZ and SZ. Integrated analysis of LP-ArGA, HPMI, NMR and SEM observation suggests a substantial decrease in the contribution of micropores to total pore volume (PV) and a concurrent increase in larger pores (meso-macropores) with the increase of weathering intensity. This results in smoother surfaces of micro-transition pores but rougher surfaces of macropores. Changes in mineralogy composition during weathering play a crucial role in influencing pore structure of shales and further accelerating the release and migration of toxic elements in black shale. Our study provides the essential theoretical foundation for the remediation of soil and water environmental pollution caused by black shale weathering.
PubMed: 38802003
DOI: 10.1016/j.scitotenv.2024.173533 -
Saudi Journal of Biological Sciences Jul 2024The inorganic colour layer based on iron oxide is affected by microorganisms (fungi) and leads to its deterioration due to feeding on the mineral elements through the...
The inorganic colour layer based on iron oxide is affected by microorganisms (fungi) and leads to its deterioration due to feeding on the mineral elements through the chemical composition of the colour in the presence of a suitable environment (medium). Damage occurs as a result of heavy metal elements being removed from the colour, leading to a defect in the chemical composition and the fading of the colours. The current study showed the effect of the different types of the most common fungi on oil paintings ( and ) after cultivating the different types of fungi and obtaining pure colonies for each fungus separately and conducting a fungal infection on experimental samples with preparing the old techniques, coloured with hematite red and goethite yellow. Each colour is mixed with different proportions of linseed oil (1, 2, 3). They were aged artificially and incubated at a temperature of 26 degrees and examined periodically until the fungi appeared on the surface in the form of colour spots ranging from very dark (severe infestation) to light (low infestation). The change in chemical composition was measured by Raman and EDX analyses of the samples before and after infection. Fungi showed the appearance of spoilage products from metal sulfides and metal carboxylates. The iron oxide ion decreased in both the red and the yellow colours, leading to a change after the fungal infection. Examination of the morphological surface using SEM, USB and measurement of colour change showed the change in the red colour more than the yellow and scattering of green and black colour dots on the surface of the sample. Correlation and Simple Linear Regression were applied for each colour before and after besides both colours together. It was found that these colours appeared around some of the fungal colonies as a result of the activities. Fungal species of some strains reduced Fe to Fe . This provides new insights into the role of microorganisms in the deterioration of painted surfaces.
PubMed: 38799718
DOI: 10.1016/j.sjbs.2024.104004