-
Journal of Clinical Microbiology Mar 1988We determined phenotypic characteristics, cellular fatty acid composition, and isoprenoid quinone content of representative strains of CDC groups EO-2, M-5, and M-6,...
Cultural and chemical characterization of CDC groups EO-2, M-5, and M-6, Moraxella (Moraxella) species, Oligella urethralis, Acinetobacter species, and Psychrobacter immobilis.
We determined phenotypic characteristics, cellular fatty acid composition, and isoprenoid quinone content of representative strains of CDC groups EO-2, M-5, and M-6, Moraxella (Moraxella) species, Oligella urethralis, Acinetobacter species, and Psychrobacter immobilis. All organisms contained ubiquinone with eight isoprene units as the major isoprenolog, but distinct differences were observed in fatty acid composition. Twenty-eight of the original collection of CDC group EO-2 strains were further identified as P. immobilis, EO-2, or EO-3 by distinctive cellular fatty acid profiles, cellular morphology, and pigment production. The cellular fatty acid compositions of M-5 and M-6 were similar but were clearly different from those of other organisms. The genus Acinetobacter was differentiated from other organisms in the study by small amounts of 2-hydroxydodecanoic acid (2-OH-12:0), and P. immobilis was differentiated by small amounts of decanoic acid (10:0) and a branched-chain 17-carbon acid (i-17:0). All Moraxella species were distinguished by small amounts of decanoic acid (10:0) and the absence of i-17:0. M. bovis, M. nonliquefaciens, and some strains of M. lacunata formed a single fatty acid group, while M. osloensis, M. phenylpyruvica, M. atlantae, and other strains of M. lacunata (M. lacunata II) had species-specific fatty acid profiles. O. urethralis differed from Moraxella species by the presence of large amounts (49%) of cis-vaccenic acid (18:1 omega 7c), small amounts (1%) of 3-hydroxyhexadecanoate (3-OH-16:0), and the absence of 10:0 and 3-hydroxydodecanoate (3-OH-12:0). The combined use of chemical data and a small number of conventional tests permitted rapid identification and differentiation of these organisms from each other and from related organisms.
Topics: Acinetobacter; Animals; Chromatography, Gas; Fatty Acids; Gram-Negative Bacteria; Humans; Minicomputers; Moraxella; Software; Transformation, Bacterial
PubMed: 3356788
DOI: 10.1128/jcm.26.3.484-492.1988 -
Journal of General Microbiology Sep 1977Forty-five strains classified in the family Neisseriaceae were analysed for wax esters by gas-liquid chromatography. The amounts and types of waxes varied between the...
Forty-five strains classified in the family Neisseriaceae were analysed for wax esters by gas-liquid chromatography. The amounts and types of waxes varied between the taxa. Waxes were not detected in 16 strains of 'true neisseriae' (genus Neisseria) or in two strains of Kingella, but they were found in all 'false neisseriae', in all species of Moraxella except Moraxella phenylpyrouvica, in five out of 10 strains of Acintobacter, and in all strains of a group of psychrophilic, oxidase-positive organisms. The chain lengths of the wax esters ranged from C24 to C42, with C36 predominating. In all taxa, esters with even numbers of carbon atoms constituted 70 to 100% of the total. Saturated, mono-unsaturated and diunsaturated waxes were found. Acinetobacter strains were characterized by large amounts (30 to 98%) of di-unsaturated wax esters; such waxes did not exceed 8% in the 'false neisseriae' or Moraxella spp. Waxes of strains belonging to the psychrophilic, oxidase-positive group generally resembled those found in Moraxella. Wax esters with odd numbers of carbon atoms were abundant in M. lacunata (29%), M. atlantae (15%) and in the psychorophilic group (19 to 28%); long-chain esters (C40 or above) were characteristic of M. atlantae (30%) and one strain of M. osloensis (26%).
Topics: Chromatography, Gas; Neisseriaceae; Species Specificity; Waxes
PubMed: 915475
DOI: 10.1099/00221287-102-1-33 -
Acta Pathologica Et Microbiologica... Aug 1976Sixty-four strains of Neisseria, Moraxella, and Acinetobacter were screened for cellular monosaccharides by gas-liquid chromatography and other chromatographic...
Sixty-four strains of Neisseria, Moraxella, and Acinetobacter were screened for cellular monosaccharides by gas-liquid chromatography and other chromatographic techniques. The four sugars ribose, glucose, glucosamine, and 2-keto-3-deoxyoctonate (KDO) were detected in all strains. Heptose was detected only in "true neisseriae" (Neisseria gonorrhoeae, N. meningitidis, N. sicca, N. cinerea, N. flavescens, and N. elongata) and in the tentaively named species Moraxella urethralis. Some marked interspecies dissimilarities within groups were revealed. Thus, N. ovis and M. atlantae were characterized by the presence of mannose. Intraspecies differences were also encountered. N. meningitidis strains of serogroups B and C were distinguished from strains of serogroup A by their sialic acid content. This sugar was also detected in two out of three examined strains of M. nonliquefaciens. In Acinetobacter, heterogeneity of monosaccharide patterns was rather pronounced. The results show the applicability of gas chromatographic "monosaccharide" profiles fo whole cells or extracted carbohydrate in bacterial classification and identification, including differentiation at the subspecies level. In addition, such profiles may be useful for monitoring during purification of cellular polysaccharides.
Topics: Acinetobacter; Chromatography, Gas; Chromatography, Paper; Chromatography, Thin Layer; Galactose; Glucosamine; Glucose; Heptoses; Ketoses; Mannose; Monosaccharides; Moraxella; Neisseria; Rhamnose; Ribose; Sialic Acids; Sugar Acids
PubMed: 961430
DOI: 10.1111/j.1699-0463.1976.tb01923.x