-
Prikladnaia Biokhimiia I Mikrobiologiia 2007Morphological and biochemical properties of the nitrogen-fixing strain Ochrobactrum intermedium ANKI, intensely growing on media with azo compounds, and its resistance...
Morphological and biochemical properties of the nitrogen-fixing strain Ochrobactrum intermedium ANKI, intensely growing on media with azo compounds, and its resistance to various common xenobiotics were investigated. The kinetics of azobenzene conversion by O. intermedium ANKI was studied. Under cometabolism conditions, up to 40 mg of azobenzene per liter of medium were decolorized within one week. It was shown that the strain possessed molybdenum-dependent nitrogenase activity, and its nitrogenase system was sensitive to oxygen and fixed nitrogen in the medium.
Topics: Azo Compounds; Color; Culture Media; Nitrogen Fixation; Ochrobactrum; Pesticides; Xenobiotics
PubMed: 17929573
DOI: No ID Found -
International Journal of Systematic and... May 2007Three novel Gram-negative, non-fermenting aerobic bacilli were isolated from human clinical samples. They shared more than 99.8 % of the 16S rRNA gene nucleotide...
Three novel Gram-negative, non-fermenting aerobic bacilli were isolated from human clinical samples. They shared more than 99.8 % of the 16S rRNA gene nucleotide positions. The strains were related to Ochrobactrum intermedium with about 97.48 % 16S rRNA gene sequence similarity. In 16S rRNA gene-, dnaK- and rpoB-based phylogenies, the strains were grouped in a lineage that was distinct from other Ochrobactrum species in the family Brucellaceae. Fatty acid composition, polar lipids, quinone system, DNA-DNA relatedness, genome organization, and physiological and biochemical data differentiated these isolates from recognized species of the genus Ochrobactrum. The three clinical strains therefore represent a novel species within the genus Ochrobactrum, for which the name Ochrobactrum pseudintermedium sp. nov., is proposed. The type strain is ADV31(T) (=CIP 109116(T)=DSM 17490(T)). The DNA G+C content of strain ADV31(T) was 54.5 mol%.
Topics: Bacterial Proteins; Bacterial Typing Techniques; DNA, Bacterial; DNA, Ribosomal; DNA-Directed RNA Polymerases; Fatty Acids; Genes, rRNA; Gram-Negative Bacterial Infections; Humans; Membrane Lipids; Molecular Sequence Data; Ochrobactrum; Phylogeny; Quinones; RNA, Bacterial; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Sequence Homology, Nucleic Acid
PubMed: 17473249
DOI: 10.1099/ijs.0.64416-0 -
Journal of Industrial Microbiology &... Aug 2007Ochrobactrum intermedium DN2 was used to degrade nicotine in tobacco waste extracts. The optimal temperature and pH of nicotine degradation by strain DN2 was 30-37...
Ochrobactrum intermedium DN2 was used to degrade nicotine in tobacco waste extracts. The optimal temperature and pH of nicotine degradation by strain DN2 was 30-37 degrees C and 7.0, respectively. Under these optimal conditions, the average degradation rate of nicotine in a 30L fed-batch culture was 140.5 mg 1(-1) h(-1). The results of this study indicate that strain DN2 may be useful for reducing the nicotine content of reconstituted tobacco.
Topics: Biodegradation, Environmental; Fermentation; Hydrogen-Ion Concentration; Industrial Waste; Nicotine; Nicotinic Agonists; Ochrobactrum; Temperature; Nicotiana
PubMed: 17333093
DOI: 10.1007/s10295-007-0212-x -
Letters in Applied Microbiology Oct 2006This study assessed the plant growth-promoting ability of the bacterial strains Ochrobactrum intermedium (isolate CrT-1) and Bacillus cereus (isolate S-6).
AIMS
This study assessed the plant growth-promoting ability of the bacterial strains Ochrobactrum intermedium (isolate CrT-1) and Bacillus cereus (isolate S-6).
METHODS AND RESULTS
Two chromium resistant bacterial strains isolated from chromium-contaminated wastewater and soils were identified as O. intermedium CrT-1 and B. cereus S-6. These strains were inoculated on seeds of mungbean Vigna radiata var NM-92, which were germinated and grown under chromate salts (300 microg ml(-1) of CrCl(3)or K(2)CrO(4)). The data show that Cr(VI) was more toxic because of its better availability to plants roots when compared with Cr(III). The major part of Cr(VI) supplied to the seedlings was reduced to Cr(III) in the rhizosphere by the bacterial strains, thus lowering the toxicity of chromium to seedlings.
CONCLUSIONS
Strains have significant Cr(VI) resistance and reduction potential and have ability to enhance mungbean plant growth under chromium stress.
SIGNIFICANCE AND IMPACT OF THE STUDY
These strains could be utilized for the growth of economically important cash crops as well as for the bioremediation of chromium-polluted soils.
Topics: Bacillus cereus; Chromium; Drug Resistance, Bacterial; Fabaceae; Hydrogen-Ion Concentration; Ochrobactrum; Plant Roots; RNA, Ribosomal, 16S; Seedlings
PubMed: 16965380
DOI: 10.1111/j.1472-765X.2006.01977.x -
Journal of Applied Microbiology Sep 2006To optimize a medium for nicotine degradation by Ochrobactrum intermedium DN2 in presence of yeast extract, glucose and Tween 80 using response surface methodology (RSM).
AIMS
To optimize a medium for nicotine degradation by Ochrobactrum intermedium DN2 in presence of yeast extract, glucose and Tween 80 using response surface methodology (RSM).
METHODS AND RESULTS
In this study, the effects of yeast extract, glucose and Tween 80 on nicotine degradation were investigated in flasks using a novel nicotine-degrading bacterium, O. intermedium DN2. A full factorial central composite design was applied in the design of experiments and in the analysis of the experimental data. The results showed that the most significant variable influencing nicotine degradation was yeast extract, followed by glucose, and then Tween 80. Moreover these three factors interacted with each other and combined to produce positive effects on nicotine degradation. The experimental data also allowed the development of an empirical model (P < 0.0001) describing the inter-relationship between independent and dependent variables. By solving the regression equation, the optimal values of the variables were determined as: yeast extracts 0.094%, glucose 0.101% and Tween 80 0.080%. Using the medium obtained, about 1,220 mg l(-1) of nicotine was degraded (95.55%) within 10 h at the specific biodegradation of 116.59 mg l(-1) h(-1) in 30-l bioreactor containing 25-l tobacco extract.
CONCLUSIONS
An optimal medium of nicotine degradation by the strain DN2 was obtained.
SIGNIFICANCE AND IMPACT OF THE STUDY
RSM proved to be reliable in developing the model, optimizing factors and analysing interaction effects. The results provide better understanding on the interactions between yeast extract, glucose and Tween 80 for nicotine biodegradation.
Topics: Biodegradation, Environmental; Culture Media; Glucose; Models, Biological; Nicotine; Nicotinic Agonists; Ochrobactrum; Polysorbates; Surface-Active Agents; Yeasts
PubMed: 16907819
DOI: 10.1111/j.1365-2672.2006.02929.x -
FEMS Microbiology Ecology May 2006The diversity of Ochrobactrum anthropi, Ochrobactrum intermedium, Ochrobactrum tritici and Ochrobactrum grignonense in agricultural soil and on the wheat rhizoplane was...
The diversity of Ochrobactrum anthropi, Ochrobactrum intermedium, Ochrobactrum tritici and Ochrobactrum grignonense in agricultural soil and on the wheat rhizoplane was investigated. O. anthropi was isolated both from soil and from the rhizoplane, O. intermedium and grignonense only from bulk soil, and O. tritici only from the wheat rhizoplane. On the genetic level, the immunotrapped isolates and a number of strains from culture collection mainly of clinical origin were compared with rep-PCR profiling using BOX primers, and a subset of these isolates and strains using REP primers. The isolates clustered according to their species affiliation. There was no correlation between rep clusters of O. anthropi isolates and habitat (place of isolation). The genetic diversity of Ochrobactrum at the species level as well as microdiversity of O. anthropi (number of BOX groups) was higher in soil than on the rhizoplane. Similarity values from genetic rep-PCR profiles correlated positively with DNA-DNA reassociation percentages. Isolates with >80.7% similarity in BOX profile and >86.4% in rep profile clustered within the same species. Similarity analysis of rep-PCR profiles is hence an alternative to DNA-DNA hybridization as a genomic criterion for species delineation within the genus Ochrobactrum. We used the substrate utilization system BIOLOG-GN to compare the immunotrapped isolates on the phenetic level. For the isolates from bulk soil, substrate utilization versatility (number of utilized substrates) and substrate utilization capacity (mean conversion rate of substrates) were slightly but significantly higher than for the isolates from the rhizoplane. This trend was also seen using API 20E and 20NE systems. Plate counts of total bacteria and the number of immunotrapped Ochrobactrum isolates per gram dry weight were higher for the rhizoplane than for the soil samples. The results of genetic and phenotypic analyses indicated a 'rhizosphere effect'; the diversity and metabolic capacity of Ochrobactrum isolates were higher in bulk soil, and the population density was higher on the wheat rhizoplane.
Topics: Biodiversity; Cluster Analysis; Genotype; Nucleic Acid Hybridization; Ochrobactrum; Phenotype; Polymerase Chain Reaction; Soil Microbiology; Triticum
PubMed: 16629756
DOI: 10.1111/j.1574-6941.2005.00029.x -
Journal of Clinical Microbiology Mar 2006Ochrobactrum intermedium [corrected] infection is rare in humans and is generally associated with immunocompromised hosts with indwelling foreign bodies. We report a... (Review)
Review
Ochrobactrum intermedium [corrected] infection is rare in humans and is generally associated with immunocompromised hosts with indwelling foreign bodies. We report a case of pelvic abscess with O. intermedium [corrected] after a routine appendectomy in an immunocompetent patient and review the literature on O. intermedium [corrected] infection in patients with normal immune function.
Topics: Abscess; Appendectomy; Gram-Negative Bacterial Infections; Humans; Immunocompetence; Male; Middle Aged; Ochrobactrum anthropi; Pelvic Infection; Postoperative Complications
PubMed: 16517927
DOI: 10.1128/JCM.44.3.1184-1186.2006 -
Bioresource Technology Jan 2007A Cr(VI) resistant bacterial strain SDCr-5, identified as Ochrobactrum intermedium on the basis of 16S rRNA gene sequencing, was tolerant to high concentrations of...
A Cr(VI) resistant bacterial strain SDCr-5, identified as Ochrobactrum intermedium on the basis of 16S rRNA gene sequencing, was tolerant to high concentrations of Cr(VI) up to 15 mg ml(-1) in acetate minimal medium. O. intermedium SDCr-5 reduced Cr(VI) under a wide range of concentrations from 100 to 1500 microg ml(-1) and reduction was optimum at 37 degrees C and pH 7. It reduced 200 and 721 microg ml(-1) Cr(VI) within 72 and 96 h, respectively. The rate of Cr(VI) reduction increased with concentration from 100 to 1500 microg ml(-1). The presence of heavy metal cations such as Cu(2+), Co(2+), Mn(2+) and Ni(2+) stimulated Cr(VI) reduction. Strain SDCr-5 might be useful for Cr(VI) detoxification under a wide range of environmental conditions.
Topics: Biodegradation, Environmental; Chromium; Hydrogen-Ion Concentration; Ochrobactrum; Phylogeny; Temperature
PubMed: 16488604
DOI: 10.1016/j.biortech.2005.12.025 -
International Journal of... 2005This study deals with the use of three chromium-resistant bacterial strains (Ochrobactrum intermedium CrT-1, Brevibacterium CrT-13, and CrM-1) in conjunction with...
This study deals with the use of three chromium-resistant bacterial strains (Ochrobactrum intermedium CrT-1, Brevibacterium CrT-13, and CrM-1) in conjunction with Eichornia crassipes for the removal of toxic chromium from wastewater. Bacterial strains resulted in reduced uptake of chromate into inoculated plants as compared to noninoculated control plants. In the presence of different heavy metals, chromium uptake into the plants was 28.7 and 7.15% less at an initial K2CrO4 concentration of 100 and 500 microg ml(-1) in comparison to a metal free chromium solution. K2CrO4 uptake into the plant occurred at different pHs tested, but maximum uptake was observed at pH 5. Nevertheless, the bacterial strains caused some decrease in chromate uptake into the plants, but the combined effect of plants and bacterial strains conduce more removal of Cr(VI) from the solution.
Topics: Brevibacterium; Chromates; Hydrogen-Ion Concentration; Ochrobactrum; Oxidation-Reduction; Water Pollutants, Chemical
PubMed: 16463540
DOI: 10.1080/16226510500327111 -
Systematic and Applied Microbiology Jun 2006The internal 16S/23S rDNA (rrs/rrl) internal spacer region 1 (ITS1) of 54 Ochrobactrum strains and close relatives was analysed. Separation of ITS1 containing PCR... (Comparative Study)
Comparative Study
The internal 16S/23S rDNA (rrs/rrl) internal spacer region 1 (ITS1) of 54 Ochrobactrum strains and close relatives was analysed. Separation of ITS1 containing PCR products by gel-electrophoresis, DGGE, cloning and sequencing revealed ITS1 length and sequence heterogeneity. We found up to 5 different allelic ITS1 stretches within a single strain (Ochrobactrum intermedium LMG 3301T), and 2-3 different ITS1 alleles in O. tritici. Within ITS1, ITS1c, being part of the conserved double-stranded rrn processing stem dsPS1, produced the most reliable segment tree. The overall ITS1, ITS1c and rrs phylogenetic tree topologies were generally consistent, but there was evidence for horizontal rrn (segment) transfer in O. tritici LMG 2134 (formerly O. anthropi). Good correlations were found between ITS1, ITS1c and rrs sequence similarity and DNA-DNA hybridization values indicating that phylogenetic analysis of ITS1 and ITS1c both can be used to preliminarily deduce the phylogenetic affiliation if HGT was excluded. Strains sharing > 96.19% ITS1c (> 95.11% ITS1) similarity fell within a species, and < or = 68.42% ITS1c (< or = 70.33% ITS1) similarity outside a genus. Both ITS1 and ITS1c analysis resolved microdiversity more profoundly than rrs analysis and revealed clades (genomovars) within O. anthropi that were also produced in rep cluster analysis. There was no evidence for habitat-specific ITS1 genomovars within Ochrobactrum species. Diversity of Ochrobactrum was higher in soil than at the rhizoplane below and at the species level. Isolates from soil contained only 1 rrn type whereas isolates from human clinical, animal and rhizoplane specimens could contain more.
Topics: Alleles; DNA, Bacterial; DNA, Intergenic; DNA, Ribosomal; Gene Transfer, Horizontal; Genes, rRNA; Molecular Sequence Data; Nucleic Acid Hybridization; Ochrobactrum; Phylogeny; Polymorphism, Genetic; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Statistics as Topic
PubMed: 16352412
DOI: 10.1016/j.syapm.2005.11.003