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Food & Function Jun 2024Red palm oil, a natural repository abundant in tocotrienols, tocopherols and carotenoids, is frequently employed as a pigment and nutritional enhancer in food products....
Red palm oil, a natural repository abundant in tocotrienols, tocopherols and carotenoids, is frequently employed as a pigment and nutritional enhancer in food products. The principal aim of this study is to explore the disparities in vitamin A levels, fatty acid profiles and gut microbiota among healthy adults who consume carotenoid-enriched eggs compared to those who consume normal eggs. A total of 200 hens were randomly assigned to either the red palm oil group or the soybean oil group, with the objective of producing carotenoid-enriched eggs and normal eggs. Throughout a six-month, double-blinded, randomized controlled trial, participants were instructed to consume one carotenoid-enriched or normal egg daily at a fixed time. Fecal and blood samples were collected from the participants at the start and conclusion of the six-month intervention period for further analysis. Our findings indicated that there was no significant change in the vitamin A level for daily supplementation with one carotenoid-enriched egg, but there were significant changes in some indicators of fatty acid profiles and gut microbiota compared to the control group of the population. Nonetheless, the consumption of eggs, regardless of carotenoid-enriched eggs or normal eggs, positively influenced dietary habits by reducing the intake of saturated fatty acids and enhancing the intake of monounsaturated and polyunsaturated fatty acids of the population.
PubMed: 38864191
DOI: 10.1039/d4fo00910j -
World Journal of Microbiology &... Jun 2024Anaerobic digestion (AD) emerges as a pivotal technique in climate change mitigation, transforming organic materials into biogas, a renewable energy form. This process...
Anaerobic digestion (AD) emerges as a pivotal technique in climate change mitigation, transforming organic materials into biogas, a renewable energy form. This process significantly impacts energy production and waste management, influencing greenhouse gas emissions. Traditional research has largely focused on anaerobic bacteria and methanogens for methane production. However, the potential of anaerobic lignocellulolytic fungi for degrading lignocellulosic biomass remains less explored. In this study, buffalo rumen inocula were enriched and acclimatized to improve lignocellulolytic hydrolysis activity. Two consortia were established: the anaerobic fungi consortium (AFC), selectively enriched for fungi, and the anaerobic lignocellulolytic microbial consortium (ALMC). The consortia were utilized to create five distinct microbial cocktails-AF0, AF20, AF50, AF80, and AF100. These cocktails were formulated based on varying of AFC and ALMC by weights (w/w). Methane production from each cocktail of lignocellulosic biomasses (cassava pulp and oil palm residues) was evaluated. The highest methane yields of CP, EFB, and MFB were obtained at 337, 215, and 54 mL/g VS, respectively. Cocktails containing a mix of anaerobic fungi, hydrolytic bacteria (Sphingobacterium sp.), syntrophic bacteria (Sphaerochaeta sp.), and hydrogenotrophic methanogens produced 2.1-2.6 times higher methane in cassava pulp and 1.1-1.2 times in oil palm empty fruit bunch compared to AF0. All cocktails effectively produced methane from oil palm empty fruit bunch due to its lipid content. However, methane production ceased after 3 days when oil palm mesocarp fiber was used, due to long-chain fatty acid accumulation. Anaerobic fungi consortia showed effective lignocellulosic and starchy biomass degradation without inhibition due to organic acid accumulation. These findings underscore the potential of tailored microbial cocktails for enhancing methane production from diverse lignocellulosic substrates.
Topics: Methane; Anaerobiosis; Lignin; Fungi; Microbial Consortia; Animals; Biomass; Rumen; Biofuels; Hydrolysis; Fermentation; Bacteria; Industrial Waste; Agriculture
PubMed: 38862848
DOI: 10.1007/s11274-024-04050-7 -
ACS Omega Jun 2024Bioenergy is predicted to significantly contribute to the global energy needs of both developed and developing economies. Co-pyrolysis of halophytes offers a solution...
Bioenergy is predicted to significantly contribute to the global energy needs of both developed and developing economies. Co-pyrolysis of halophytes offers a solution for a sustainable supply of feedstock in coastal and water-scarce regions. This novel research introduces an experimental investigation of co-pyrolysis of saline-tolerant flora (date palm waste and ) to address sustainable waste management, bioenergy production, and efficient resource utilization in xeric regions. To examine the impact of the thermic condition on the pyrolysis products (bio-oil, biochar, and gas), the experiments have been conducted at three different temperatures (400, 500, and 600 °C). This pioneering study revealed that the co-feed bio-oil is acidic (pH 3.76-4.39) and has a high energy content (HHV 32.29-36.29 MJ/kg) that surpasses most woody biomass. The produced biochar was chemically stable, high in ash (40.09-47.62 wt %), high in fixed carbon (30.12-38.12 wt %), highly alkaline (pH 9.37-10.69), and low in HHV (16.30-17.2 MJ/kg). Increased pyrolysis temperature enhances biochar stability and fixed carbon, thus benefiting long-term carbon sequestration if applied in the soil. However, due to its high alkalinity, the application of this biochar in naturally alkaline sandy soils, such as in coastal deserts, requires careful monitoring. The hydrogen content in the gaseous phase significantly improves with rising temperature, reaching HHV = 24.12 MJ/kg at 600 °C, due to the enhanced ash catalytic effect. Overall, this study constitutes an important contribution to advancing bioenergy, sustainable feedstock, carbon capture, and waste management strategies in drought-prone areas.
PubMed: 38854508
DOI: 10.1021/acsomega.4c02972 -
PloS One 2024This research presents a comprehensive study of sequential oxidative extraction (SOE) consisting of alkaline and acidic oxidation processes to extract nanocellulose from...
This research presents a comprehensive study of sequential oxidative extraction (SOE) consisting of alkaline and acidic oxidation processes to extract nanocellulose from plant biomass. This proposed process is advantageous as its operation requires a minimum process with mild solvents, and yet successfully isolated high-quality nanofibrillated cellulose (NFC) from raw OPEFB. The SOE involved ammonium hydroxide (NH4OH, 2.6 M) and formic acid (HCOOH, 5.3 M) catalyzed by hydrogen peroxide (H2O2, 3.2 M). This approach was used to efficiently solubilize the lignin and hemicellulose from Oil Palm Empty Fruit Bunch (OPEFB) at the temperature of 100°C and 1 h extraction time, which managed to retain fibrous NFC. The extracted solid and liquor at each stage were studied extensively through physiochemical analysis. The finding indicated that approximately 75.3%dwb of hemicellulose, 68.9%dwb of lignin, and 42.0%dwb of extractive were solubilized in the first SOE cycle, while the second SOE cycle resulted in 92.3%dwb, 99.6%dwb and 99.8%dwb of solubilized hemicellulose, lignin, and extractive/ash, respectively. High-quality NFC (75.52%dwb) was obtained for the final extracted solid with 76.4% crystallinity, which is near the crystallinity of standard commercial NFC. The proposed process possesses an effective synergy in producing NFC from raw OPEFB with less cellulose degradation, and most of the degraded hemicellulose and lignin are solubilized in the liquor.
Topics: Cellulose; Fruit; Oxidation-Reduction; Arecaceae; Lignin; Nanofibers; Palm Oil; Polysaccharides; Hydrogen Peroxide
PubMed: 38843202
DOI: 10.1371/journal.pone.0299312 -
Pakistan Journal of Biological Sciences... Apr 2024<b>Background and Objective:</b> The prioritisation of oil palm studies involves the exploration of novel bacterial isolates as possible agents for...
<b>Background and Objective:</b> The prioritisation of oil palm studies involves the exploration of novel bacterial isolates as possible agents for suppressing <i>Ganoderma boninense</i>. The objective of this study was to evaluate and characterise the potential of rhizospheric bacteria, obtained from the rhizosphere of oil palm plants, in terms of their ability to demonstrate anti-<i>Ganoderma </i>activity. <b>Materials and Methods:</b> The study began by employing a dual culture technique to select hostile bacteria. Qualitative detection was performed to assess the antifungal activity, as well as the synthesis of chitinase and glucanase, from certain isolates. The candidate strains were molecularly identified using 16S-rRNA ribosomal primers, specifically the 27F and 1492R primers. <b>Results:</b> The findings of the study indicated that the governmental plantation exhibited the highest ratio between diazotroph and indigenous bacterial populations in comparison to the other sites. Out of a pool of ninety bacterial isolates, a subset of twenty-one isolates demonstrated the ability to impede the development of <i>G. boninense</i>, as determined using a dual culture experiment. Twenty-one bacterial strains were found to exhibit antifungal activity. Nine possible bacteria were found based on the sequence analysis. These bacteria include <i>Burkholderia territorii</i> (RK2, RP2, RP3, RP5), <i>Burkholderia stagnalis</i> (RK3), <i>Burkholderia cenocepacia</i> (RP1), <i>Serratia marcescens</i> (RP13) and <i>Rhizobium multihospitium</i> (RU4). <b>Conclusion:</b> The findings of the study revealed that a significant proportion of the bacterial population exhibited the ability to perform nitrogen fixation, indole-3-acetic acid (IAA) production and phosphate solubilization. However, it is worth noting that <i>Rhizobium multihospitium</i> RU4 did not demonstrate the capacity for phosphate solubilization, while <i>B. territory</i> RK2 did not exhibit IAA production.
Topics: Ganoderma; Rhizosphere; Biological Control Agents; Bioprospecting; Soil Microbiology; Bacteria; Arecaceae; Plant Development; Palm Oil; Antifungal Agents
PubMed: 38840466
DOI: 10.3923/pjbs.2024.256.267 -
Journal of Environmental Quality Jun 2024Biochar produced from lignocellulosic biomass offers an opportunity to recycle waste into a valuable soil amendment. The application of biochar has been proposed to...
Biochar produced from lignocellulosic biomass offers an opportunity to recycle waste into a valuable soil amendment. The application of biochar has been proposed to mitigate climate change by sequestering carbon in the soil. However, the field impact of biochar treatment on the cellulolytic microbial populations involved in the earlier steps of cellulose degradation is poorly understood. A field trial spanning three consecutive crop cycles of Zea mays was conducted in a degraded tropical Ultisol of Peninsular Malaysia. The soil was amended with two contrasting biochar made from oil palm kernel shells (pyrolyzed at 400°C) and rice husks (gasified at 800°C) with or without fertilizer supplementation. Soil samples were taken at each harvesting stage and analyzed for total organic carbon, labile active organic carbon, total cellulase, and β-glucosidase. Microbial glycoside hydrolase family 6 (GH6) cellulase genes and transcripts, involved in the early steps of cellulose degradation, were quantified from the extracted soil deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), respectively. Total organic carbon, labile active organic carbon, and β-glucosidase activity were significantly increased, while no effect on total cellulase activity was found. Both biochars stimulated the total population (DNA-derived) abundance of soil microorganisms harboring the GH6 cellulase genes. The biochar amendment did not affect the active population (RNA-derived) of the GH6 cellulolytic community, showing no significant changes in transcript expression. This indirectly corroborates the role of biochar as a potential carbon sequester in the soil.
PubMed: 38840421
DOI: 10.1002/jeq2.20588 -
Analytica Chimica Acta Jul 2024Mineral oil aromatic hydrocarbon (MOAH) analysis in foods is a major analytical challenge. Quantification is associated with a high uncertainty. The sources of...
BACKGROUND
Mineral oil aromatic hydrocarbon (MOAH) analysis in foods is a major analytical challenge. Quantification is associated with a high uncertainty. The sources of uncertainty are multiple, but the major one is related to data interpretation and integration, which is partially derived from insufficiently efficient sample preparation. Recently, an updated ISO method for the analysis of mineral oil in fats and oils and a standard operating procedure for infant formula analysis have been published. Both methods reported significantly different (up to 1.25) distributions of the internal standards used for quantification (i.e., tri-tert-butyl benzene (TBB) and 2-methyl naphthalene (2-MN)) over the different solvent phases used in the saponification step.
RESULTS
In this work, a microwave-assisted saponification and extraction method was optimized for MOAH analysis to solve the problem related to the MOAH internal standards partition. The paper examines the impact of the solvent mixture used, the concentration of KOH on the partition of TBB and 2-MN, and the effect of the matrix and the washing step to extract the unsaponifiable fraction containing the mineral oils.
SIGNIFICANCE
The optimized procedure achieved a TBB/2-MN ratio of 1.05 ± 0.01 tested in five different fats and oils, namely, sunflower, rapeseed, coconut, palm, and extra virgin olive oils. The method can significantly contribute to reducing the uncertainty of the MOAH quantification when saponification is applied.
Topics: Microwaves; Hydrocarbons, Aromatic; Mineral Oil
PubMed: 38834273
DOI: 10.1016/j.aca.2024.342788 -
European Journal of Nutrition Jun 2024To describe adherence to sustainable healthy diets among a sample of 958 Chilean pre-schoolers (3-6 years) and explore associations between adherence and child and...
PURPOSE
To describe adherence to sustainable healthy diets among a sample of 958 Chilean pre-schoolers (3-6 years) and explore associations between adherence and child and maternal sociodemographic and anthropometric characteristics.
METHODS
Children's adherence to sustainable healthy diets was calculated from single multiple-pass 24-h dietary recalls using the Planetary Health Diet Index for children and adolescents (PHDI-C). Higher PHDI-C scores (max score = 150 points) represent greater adherence. Adjusted linear regression models were fitted to explore associations between PHDI-C scores and child and maternal characteristics.
RESULTS
Children obtained low total PHDI-C scores (median 50.0 [IQR 39.5-59.8] points). This resulted from low consumption of nuts & peanuts, legumes, vegetables, whole cereals, and vegetable oils; a lack of balance between dark green and red & orange vegetables, inadequate consumption of tubers & potatoes and eggs & white meats, and excess consumption of dairy products, palm oil, red meats, and added sugars. Mean PHDI-C total score was significantly higher (50.6 [95%CI 49.6, 51.7] vs 47.3 [95%CI 45.0, 49.5]) among children whose mothers were ≥ 25 years compared to those with younger mothers. Positive associations were observed between scores for fruits and maternal education, vegetables and maternal age, added sugars and child weight status, while negative associations were observed between fruits and child age, and vegetable oils and maternal education. Scores for dairy products PHDI-C component were lower among girls.
CONCLUSION
Adherence to sustainable healthy diets was low among this sample of Chilean children and was significantly associated with maternal age, being lower among children whose mothers were younger.
PubMed: 38829558
DOI: 10.1007/s00394-024-03435-6 -
Food and Chemical Toxicology : An... Jul 2024Edible plants samples were analysed for non-carcinogenic and carcinogenic human health risks. The elements nickel (Ni), cadmium (Cd), arsenic (As), lead (Pb), chromium...
Edible plants samples were analysed for non-carcinogenic and carcinogenic human health risks. The elements nickel (Ni), cadmium (Cd), arsenic (As), lead (Pb), chromium (Cr) and mercury (Hg) analysed using atomic absorption spectrophotometer (AAS). The recovery, limit of detection (LOD) and limit of quantification (LOQ) ranged from 75 to 89 %, 0.001-0.003 and 0.003-0.01, respectively. The mean value of Hg (0.34 mg/kg) exceeded the limit of 0.05 mg/kg recommended by World Health Organization (WHO). The estimated daily intake (EDI) of Cd in adults ranged from 7.93 × 10 to 1.43 × 10 and that of Hg from 0.07 to 1.27 and Cd (0.08 × 10) in children. These are below the oral reference doses (RfD). Hazard Quotient (HQ) of Hg in children was 1.92. The elements Hg and As obtained the highest total hazard (THI) index of 2.02 in mango1 and oil palm. Arsenic possessed the highest cancer risk of 4.5 × 10 in children and 1.9 × 10 in adults. Cancer risk (CR) ranged from low to moderate (10-10), which is below the limit of 10. The total carcinogenic risk (TCR) of the edible plants were above the limit of 10. The study identified minimal As and Hg pollution and carcinogenic risks in edible plants.
Topics: Humans; Plants, Edible; Risk Assessment; Food Contamination; Child; Arsenic; Adult; Metals, Heavy; Spectrophotometry, Atomic
PubMed: 38824991
DOI: 10.1016/j.fct.2024.114760 -
Food Research International (Ottawa,... Jul 2024In this paper, two emulsion systems with high and low solid fat contents were prepared from 20 % water phase and 80 % oil phase by adjusting the palm oil/palm...
In this paper, two emulsion systems with high and low solid fat contents were prepared from 20 % water phase and 80 % oil phase by adjusting the palm oil/palm stearin/soybean oil ratio. Different ultrasonic power and time were used for the pretreatment of emulsion with different solid fat content, and the application characteristics of ultrasonic in W/O emulsions were explored and evaluated. Directly using high-intensity ultrasound to prepare fatty emulsions would weaken the hardness and storage modulus G' of the samples. Although ultrasound reduced the size of fat crystals in emulsions, the interaction between water droplets and fat crystals needs to be considered. After ultrasonic treatment, water droplets were difficult to immobilize on the crystal surface and thus acted as an active filler to stabilize the emulsion together with the fat crystal network. In high solid fat emulsion systems, an increase in ultrasound power (from 100 W to 200 W) could more affect the crystallization behavior of fats than an increase in ultrasound duration (from 30 s to 60 s), and the distribution of crystals and droplets was more uniform. In the low solid fat emulsion system, the texture of the sample after ultrasonic treatment was softer, and the surface was more delicate and smoother. However, the higher ultrasonic intensity (200 W) was not conducive to the preparation of the spread. Although the ultrasound with excessive intensity promoted the formation of small crystals, it would also lead to the aggregation of small crystals. These small crystals cannot form a uniform crystal network, which increases the fluidity of emulsions.
Topics: Emulsions; Water; Crystallization; Palm Oil; Particle Size; Soybean Oil; Ultrasonic Waves; Ultrasonics
PubMed: 38823876
DOI: 10.1016/j.foodres.2024.114493