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BMC Psychiatry Jun 2024The 2022 Ebola Virus Disease (EVD) outbreak occurred at a time when Uganda was still battling the social and psychological challenges of the COVID-19 pandemic; placing...
BACKGROUND
The 2022 Ebola Virus Disease (EVD) outbreak occurred at a time when Uganda was still battling the social and psychological challenges of the COVID-19 pandemic; placing health care professionals (HCPs) at a much higher risk of developing psychological distress. Psychological distress among HCPs can cause decreased workplace productivity and ineffective management of their patients. The current study aimed to investigate and understand psychological distress among HCPS in Mbarara city in Southwestern Uganda following the 2022 EVD outbreak.
METHOD
We enrolled 200 HCPs through convenient sampling from one private and one public health facility in Mbarara city in Southwestern Uganda, in a cross-sectional convergent parallel mixed method approach where qualitative and quantitative data were collected concurrently. Quantitative data, utilizing the Kessler Psychological Distress (K10) Scale, provided us with a quantitative measure of the prevalence of psychological distress among HCPs, and were analyzed using STATA version 16. Qualitative data, on the other hand, offered deeper insights into the nature, perceptions, and contextual factors influencing this distress, and were analyzed using emergent theme analysis.
RESULTS
The prevalence of psychological distress was 59.5% and it was higher among females (63.9%) compared to males (36.1%). HCPs vividly expressed distress and anxiety, with heightened suspicion that every patient might be an EVD carrier, creating a pervasive sense of unsafety in the workplace. However, the outbreak had an educational affect where concerns about the announcement of another EVD outbreak were diverse, with HCPs expressing anxiety, despair, and dissatisfaction with the country's management of potential outbreaks.
CONCLUSION
High levels of psychological distress were experienced by HCPs in Southwestern Uganda as a result of the 2022 EVD pandemic. HCPs express a wide range of feelings, such as dread, anxiety, despair, pessimism, and discontent with the way the outbreaks are handled throughout the nation. We recommend implementation of comprehensive psychosocial support programs tailored to the unique needs of HCPs, including counseling services, stress management workshops, and peer support networks.
PubMed: 38918760
DOI: 10.1186/s12888-024-05922-w -
Nature Aging Jun 2024Susceptibility to the biological consequences of aging varies among organs and individuals. We analyzed hepatocyte transcriptomes of healthy young and aged male mice to...
Susceptibility to the biological consequences of aging varies among organs and individuals. We analyzed hepatocyte transcriptomes of healthy young and aged male mice to generate an aging hepatocyte gene signature, used it to deconvolute transcriptomic data from humans and mice with metabolic dysfunction-associated liver disease, validated findings with functional studies in mice and applied the signature to transcriptomic data from other organs to determine whether aging-sensitive degenerative mechanisms are conserved. We discovered that the signature enriches in diseased livers in parallel with degeneration. It is also enriched in failing human hearts, diseased kidneys and pancreatic islets from individuals with diabetes. The signature includes genes that control ferroptosis. Aged mice develop more hepatocyte ferroptosis and liver degeneration than young mice when fed diets that induce metabolic stress. Inhibiting ferroptosis shifts the liver transcriptome of old mice toward that of young mice and reverses aging-exacerbated liver damage, identifying ferroptosis as a tractable, conserved mechanism for aging-related tissue degeneration.
PubMed: 38918603
DOI: 10.1038/s43587-024-00652-w -
The Journal of Prosthetic Dentistry Jun 2024The effect of different surface locking patterns on the trueness of a digital implant scan of a completely edentulous arch remains uncertain.
STATEMENT OF PROBLEM
The effect of different surface locking patterns on the trueness of a digital implant scan of a completely edentulous arch remains uncertain.
PURPOSE
The purpose of this in vitro study was to evaluate whether locking surfaces with different patterns affected the trueness of complete arch implant digital scans.
MATERIAL AND METHODS
An edentulous maxillary cast with 4 implants (2 anterior implants parallel and 2 posterior implants tilted at 17 degrees) was fabricated. Four implant-level scan bodies were fixed onto the implants, and the cast was scanned with a desktop scanner to create the reference file. Four groups (L0, L1, L2, and L3) were formed, each with a distinct locking surface configuration, and all scans were made using the same intraoral scanner. L0 kept all 4 implant-level scan bodies during scanning. L1 involved removing the right first premolar (RPM) scan body, scanning the other 3 implant scan bodies, then reattaching the RPM's scan body, and continuing scanning. In L2, the RPM and right lateral incisor (RIC) scan bodies were removed, followed by scanning the left implants to create a locking surface, and scanning the right implants. In L3, only the left posterior molar's (LPM) scan body was retained and scanned; then a locking surface was generated, and then the remaining implants were scanned. A metrology software program (Geomagic Control X) was used for comparison. Statistical analyses were performed using the Kruskal-Wallis, the 1-way ANOVA, the Welch ANOVA, the Friedman test, the repeated-measures ANOVA, the Bonferroni post hoc test, and the Games-Howell post hoc test (α=.05).
RESULTS
Significant 3D surface deviations were observed in the coronal bevel (CB) region and in the entire scan bodies when assessing trueness in the L0, L1, L2, and L3 groups (P<.001). L2 exhibited the highest discrepancies in 3D surface deviation for CB (0.030 ±0.002 mm) and implant scan bodies (0.357 ±0.052 mm) and distance deviation, while the highest mean angular deviation values were found in L0 (0.924 ±0.131 degrees).
CONCLUSIONS
Locking half of the arch showed the highest trueness discrepancies when performing digital scans for complete arch implant-supported prostheses.
PubMed: 38918157
DOI: 10.1016/j.prosdent.2024.05.019 -
International Journal of Biological... Jun 2024Nasopharyngeal carcinoma (NPC), a malignant cancer originating from the epithelial cells of the nasopharynx, presents diagnostic challenges with current methods such as...
Nasopharyngeal carcinoma (NPC), a malignant cancer originating from the epithelial cells of the nasopharynx, presents diagnostic challenges with current methods such as plasma Epstein-Barr virus (EBV) DNA testing showing limited efficacy. This study focused on identifying small extracellular vesicle (sEV) proteins as potential noninvasive biomarkers to enhance NPC diagnostic accuracy. We isolated sEVs from plasma and utilized 4D label-free proteomics to identify differentially expressed proteins (DEPs) among healthy controls (NC = 10), early-stage NPC (E-NPC = 10), and late-stage NPC (L-NPC = 10). Eighteen sEV proteins were identified as potential biomarkers. Subsequently, parallel reaction monitoring (PRM) proteomic analysis preliminarily confirmed sEV carbonic anhydrase 1 (CA1) as a highly promising biomarker for NPC, particularly in early-stage diagnosis (NC = 15; E-NPC = 10; L-NPC = 15). To facilitate this, we developed an automated, high-throughput and highly sensitive CA1 immune-chemiluminescence chip technology characterized by a broad linear detection range and robust controls. Further validation in an independent retrospective cohort (NC = 89; E-NPC = 39; L-NPC = 172) using this technology confirmed sEV CA1 as a reliable diagnostic biomarker for NPC (AUC = 0.9809) and E-NPC (AUC = 0.9893), independent of EBV-DNA testing. Notably, sEV CA1 exhibited superior diagnostic performance compared to EBV-DNA, with a significant incremental net reclassification improvement of 27.61 % for NPC and 72.11 % for E-NPC detection. Thus, this study identifies sEV CA1 as an innovative diagnostic biomarker for NPC and E-NPC independent of EBV-DNA. Additionally, it establishes an immune-chemiluminescence chip technology for the detection of sEV CA1 protein, paving the way for further validation and clinical application.
PubMed: 38917926
DOI: 10.1016/j.ijbiomac.2024.133403 -
Bioinspiration & Biomimetics Jun 2024Flying insects rely mainly upon visual motion to detect and track objects. There has been a lot of research on fly inspired algorithms for object detection, but few have...
Flying insects rely mainly upon visual motion to detect and track objects. There has been a lot of research on fly inspired algorithms for object detection, but few have been developed based on visual motion alone. One of the daunting difficulties is that the neural and circuit mechanisms underlying the foreground-background segmentation are still unclear. Our previous modeling study proposed that the lobula held parallel pathways with distinct directional selectivity, each of which could retinotopically discriminate figures moving in its own preferred direction based on relative motion cues. The previous model, however, didn't address how the multiple parallel pathways gave the only detection output at their common downstream. Since the preferred directions of the pathways along either horizontal or vertical axis were opposite to each other, the background moving in the opposite direction to an object also activated the corresponding lobula pathway. Indiscriminate or ungated projection from all the pathways to their downstream would mix objects with the moving background, making the previous model fail with non-stationary background. Here, we extend the previous model by proposing that the background motion-dependent gating of individual lobula projections is the key to object detection. Large-field lobula plate tangential cells are hypothesized to perform the gating to realize bioinspired background subtraction. The model is shown to be capable of implementing a robust detection of moving objects in video sequences with either a moving camera that induces translational optic flow or a static camera. The model sheds light on the potential of the concise fly algorithm in real-world applications.
PubMed: 38917814
DOI: 10.1088/1748-3190/ad5ba3 -
Cell Research Jun 2024Migrasomes, enriched with signaling molecules such as chemokines, cytokines and angiogenic factors, play a pivotal role in the spatially defined delivery of these...
Migrasomes, enriched with signaling molecules such as chemokines, cytokines and angiogenic factors, play a pivotal role in the spatially defined delivery of these molecules, influencing critical physiological processes including organ morphogenesis and angiogenesis. The mechanism governing the accumulation of signaling molecules in migrasomes has been elusive. In this study, we show that secretory proteins, including signaling proteins, are transported into migrasomes by secretory carriers via both the constitutive and regulated secretion pathways. During cell migration, a substantial portion of these carriers is redirected to the rear of the cell and actively transported into migrasomes, driven by the actin-dependent motor protein Myosin-5a. Once at the migrasomes, these carriers fuse with the migrasome membrane through SNARE-mediated mechanisms. Inhibiting migrasome formation significantly reduces secretion, suggesting migrasomes as a principal secretion route in migrating cells. Our findings reveal a specialized, highly localized secretion paradigm in migrating cells, conceptually paralleling the targeted neurotransmitter release observed in neuronal systems.
PubMed: 38918584
DOI: 10.1038/s41422-024-00992-7 -
Scientific Reports Jun 2024In this study, synthesize and insight the corrosion inhibition properties of two novel derivatives of 1-naphthyl-2-cyanoacetamide (NCDs)...
In this study, synthesize and insight the corrosion inhibition properties of two novel derivatives of 1-naphthyl-2-cyanoacetamide (NCDs) [2-cyano-2-((5,6-dimethyl-1H-benzo[d]imidazol-2-yl) diazenyl)-N (naphthalene-1-yl)acetamide] (NCD1) and [2-Cyano-N-(naphthalene-1-yl)-2-[(4,6-dimethyl-1H-pyrazolo [3, 4-b] pyridine-3-yl) hydrazono] acetamide] (NCD2). The characterization of the synthesized NCDs was confirmed through the utilization of Mass fragmentation analysis, H-NMR, and IR. The corrosion inhibition performance of NCDs as a novel and environmentally safe corrosion inhibitor has been investigated by electrochemical techniques, a chemical technique, and theoretical studies for its anti-corrosion behavior of Inconel 800 in chloride medium. In addition, the surface morphology and inhibitor adsorption on the Inconel 800 surface were confirmed utilizing SEM, EDX, FTIR, and AFM. The advantages of NCDs include their low toxicity, environmental friendliness, ease of preparation, low odor, contain (N, O, and π-Bonds), and the inhibition efficiency elevated with decreasing solution temperature as well as inhibitor dose increase, yielding increased efficiencies of 91.8% and 95.7% for NCD1 and NCD2, respectively, at the optimum concentration of 21 10 mol. L and 298 K temperature. An analysis of Tafel plots reveals that NCDs adhere to a mixed and isothermal Langmuir adsorption mechanism. Density Functional Theory (DFT) and Monte Carlo (MC) simulation manifest the two compounds of NCDs can be adsorbed at the Fe (110) surface in a paralleled way, and can have a smaller energy gap (ΔE) value and exhibit higher efficiency. The experimental and theoretical findings confirm that the synthesized compounds obtained are capable of protecting the Inconel 800 from corrosion by creating an anti-corrosion coating on the surface.
PubMed: 38918473
DOI: 10.1038/s41598-024-62795-5 -
Journal of American College Health : J... Jun 2024This study explicates the relationship between college student's adverse childhood experiences (ACEs) and their anticipated engagement in five types of risky behaviors....
College students' adverse childhood experiences and their anticipated risky behaviors: Early maladaptive schemas and emotion regulation difficulties as potential mediators.
This study explicates the relationship between college student's adverse childhood experiences (ACEs) and their anticipated engagement in five types of risky behaviors. Two transdiagnostic mechanisms were tested cross-sectionally: disconnection/rejection early maladaptive schemas (cognitive) and difficulties in emotional regulation (emotional). 521 college student participants were majority female (66.8%), White (57.2%), freshman (54.9%), and heterosexual (72.2%). Participants completed an online, self-report, survey. Pearson bivariate correlations and parallel mediation analyses were conducted. Sixty percent of students endorsed at least one ACE. Among the total sample, disconnection/rejection schemas partially mediated the relationship between ACEs and anticipated engagement in irresponsible academic/work behaviors, drug use, aggressive/illegal activities, and heavy drinking. Difficulties in emotion regulation partially mediated anticipated risky sexual activities and irresponsible academic/work behaviors. Sex differences were noted. Preventing irresponsible academic activity and other risky behaviors is critically important to college students, parents, and administrators. Intervention efforts should address cognitive and emotional mechanisms.
PubMed: 38917370
DOI: 10.1080/07448481.2024.2369854 -
PloS One 2024Although a robust body of previous empirical studies investigated the long-term trend of child behavior problems, limited research discussed the influences of various...
Although a robust body of previous empirical studies investigated the long-term trend of child behavior problems, limited research discussed the influences of various types of neighborhood factors on such trajectory (e.g., neighborhood structural characteristics and collective efficacy). Using a nationally representative longitudinal dataset the Fragile Families and Child Wellbeing Study (FFCWS), with six waves from 1998 to 2017, this study captures the longitudinal effects of two types of early childhood neighborhood factors on the co-development of internalizing and externalizing symptoms. Data was collected at the focal child's age 3, age 5, age 9, age 15 (N = 2,385), and the parallel-process growth curve models were applied. Results suggest that the trajectories of both internalization and externalizing symptoms showed U-shape and bidirectional relationships among internalizing and externalizing problems. The long-term effects of neighborhood social cohesion and economic disadvantages were significantly associated with children's internalizing and externalizing symptoms. The implication of this study was further discussed.
Topics: Humans; Child; Residence Characteristics; Male; Adolescent; Female; Child, Preschool; Longitudinal Studies; Child Behavior
PubMed: 38917156
DOI: 10.1371/journal.pone.0305632 -
PloS One 2024The use of multi-criteria decision analysis (MCDA) for disease prioritization at the sub-national level in sub-Sahara Africa (SSA) is rare. In this research, we...
The use of multi-criteria decision analysis (MCDA) for disease prioritization at the sub-national level in sub-Sahara Africa (SSA) is rare. In this research, we contextualized MCDA for parallel prioritization of endemic zoonoses and animal diseases in The Adamawa and North regions of Cameroon. MCDA was associated to categorical principal component analysis (CATPCA), and two-step cluster analysis. Six and seven domains made of 17 and 19 criteria (out of 70) respectively were selected by CATPCA for the prioritization of zoonoses and animal diseases, respectively. The most influencing domains were "public health" for zoonoses and "control and prevention" for animal diseases. Twenty-seven zoonoses and 40 animal diseases were ranked and grouped in three clusters. Sensitivity analysis resulted in high correlation between complete models and reduced models showing the robustness of the simplification processes. The tool used in this study can be applied to prioritize endemic zoonoses and transboundary animal diseases in SSA at the sub-national level and upscaled at the national and regional levels. The relevance of MCDA is high because of its contextualization process and participatory nature enabling better operationalization of disease prioritization outcomes in the context of African countries or other low and middle-income countries.
Topics: Cameroon; Zoonoses; Animals; Humans; Decision Support Techniques; Animal Diseases; Principal Component Analysis; Cluster Analysis; Health Priorities; Public Health
PubMed: 38917073
DOI: 10.1371/journal.pone.0295742