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Infection and Drug Resistance 2022is a Gram-positive anaerobic coccus, which forms part of the normal flora and the human commensals of the skin, genitourinary system, and gut. It can cause...
is a Gram-positive anaerobic coccus, which forms part of the normal flora and the human commensals of the skin, genitourinary system, and gut. It can cause opportunistic infections in immunocompromised patients and is frequently isolated as part of polymicrobial spectra. Severe monomicrobial infections caused by the genus rarely occur. In this study, we report on septic shock, renal abscess, and bacteremia due to in a woman with nephrosis and diabetes mellitus. To the best of our knowledge, this report is the first to describe isolated from both renal abscess and blood cultures purely. The underlying diseases of the host and the removal of the double J tube were significant predisposing factors in this infection.
PubMed: 35281574
DOI: 10.2147/IDR.S353966 -
Frontiers in Microbiology 2022Previously, we demonstrated that rectal swabs provide a legitimate alternative to faecal sampling for the assessment of the intestinal microbiota in young piglets....
Previously, we demonstrated that rectal swabs provide a legitimate alternative to faecal sampling for the assessment of the intestinal microbiota in young piglets. However, we also reported that mucosa-adhered microbial populations were more represented in rectal swabs compared to faecal samples, albeit to a degree that varied per swab-sample. Here, we explored the possibility to exploit this variable enrichment of adhered populations in the rectal swabs to assess the impact of diet on mucosa-adhered microbiota in pre-weaning piglets. Paired samples of rectal swabs and colon luminal contents were collected from piglets just before weaning during two independent but similarly designed animal experiments [ = 28 piglets (experiment 1); = 16 piglets (experiment 2)], with an early feeding treatment (EF) group that had access to customised fibrous feed in addition to sow's milk and a control (CON) group exclusively reared on sow's milk. The intestinal microbiome composition in rectal swabs and colon samples collected at 29 days of age were subjected to metataxonomic analysis. The results identified the genera , , and as discriminative taxa enriched in rectal swabs compared to colon. Apart from (10-11% average relative abundance), most of these mucosa-adhered microbial genera display relatively low abundance. Rectal swab microbiota was found to be more variable, which is likely due to variable enrichment of mucosa-adhered microbes. Although almost exclusively driven by one of the experiments, the post-weaning diarrhoea-associated taxa , was enriched in CON compared to the EF group, suggesting that early life feeding may suppress post-weaning-diarrhoea-related problems in piglets. Our findings demonstrate that rectal swabs allow the investigation of the mucosa-adhered microbial populations as a function of dietary treatment in piglets. This offers opportunities to further study dietary approaches that suppress the abundance of the post-weaning diarrhoea associated adherent microbes like . Furthermore, we demonstrate that the paired swab-colon microbiota information (obtained from a subset of animals) can predict the mucosa-adhered populations or "mucosity factor" in rectal swab samples, facilitating the analysis of the adhered microbiota in large animal cohort studies using readily obtainable rectal swabs.
PubMed: 35273582
DOI: 10.3389/fmicb.2022.804986 -
International Journal of Systematic and... Mar 2022Non-human primates harbour diverse microbiomes in their guts. As a part of the China Microbiome Initiatives, we cultivated and characterized the gut microbiome of...
Non-human primates harbour diverse microbiomes in their guts. As a part of the China Microbiome Initiatives, we cultivated and characterized the gut microbiome of cynomolgus monkeys (). In this report, we communicate the characterization and taxonomy of eight bacterial strains that were obtained from faecal samples of captive cynomolgus monkeys. The results revealed that they represented eight novel bacterial species. The proposed names of the eight novel species are (type strain MSJ-5=CGMCC 1.45007=KCTC 15974), MSJd-7 (MSJd-7=CGMCC 1.45013=KCTC 25112), (MSJ-11=CGMCC 1.45009=KCTC 25065), (MSJ-4=CGMCC 1.45006=KCTC 15975), (MSJ-2=CGMCC 1.32896=KCTC 15976), MSJ-6 (MSJ-6=CGMCC 1.45008=KCTC 15973), (MSJ-1=CGMCC 1.31770=KCTC 15977) and (MSJ-40=CGMCC 1.45012=KCTC 25071).
Topics: Animals; Bacterial Typing Techniques; Base Composition; Clostridium; DNA, Bacterial; Fatty Acids; Feces; Haplorhini; Paenibacillus; Phospholipids; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA
PubMed: 35258450
DOI: 10.1099/ijsem.0.005276 -
Frontiers in Cellular and Infection... 2022Age-related changes in nasal bacterial microbiota of patients with chronic rhinosinusitis (CRS) remains unclear. In this study, we aimed to identify distinct...
Age-related changes in nasal bacterial microbiota of patients with chronic rhinosinusitis (CRS) remains unclear. In this study, we aimed to identify distinct characteristics of nasal bacterial microbiota between aged and younger patients with CRS through 16S rDNA gene sequencing. Patients with CRS undergoing endoscopic sinus surgery were recruited and separated into aged (≥60 years, median age = 66 years, N = 17) and younger (<60 years, median age = 35.5 years, N = 14) patients. Diversity, bacterial composition and metabolic activities of nasal microbiota between aged and younger patients were compared. Results have shown that levels of OTUs (p = 0.0173) and microbiota diversity (all p < 0.05) decreased significantly in aged patients. The abundance of phylum , and genus were significantly higher in aged patients, while the abundance of phylum , , and genus , were significantly higher in younger patients. In addition, predicted functional profiles have revealed that 41 KEGG pathways involving in 12 metabolic pathways, 4 genetic information processing, 3 environmental information processing, 4 cellular processes, 8 organismal systems, 6 human diseases, and 4 unclassified pathways were identified. Among which, the vast majority of metabolic activities are involved in replication and repair, membrane transport, translation, and the metabolism of amino acid, carbohydrate, energy, cofactors and vitamins, and nucleotide. On the level of the thirdly bacterial metabolic pathways, purine metabolism, glycine, serine and threonine metabolism, valine, leucine and isoleucine biosynthesis, glycolysis/gluconeogenesis and phenylalanine, tyrosine and tryptophan biosynthesis are significantly up-regulated while carbon fixation pathways in prokaryotesand methane metabolism are significantly down-regulated in aged patients. Overall, our analysis revealed that age-related physiological and pathological changes on the nasal mucosal surface may alter the host immune response and be highly associated with the nasal bacterial microbiota of patients with CRS. However, future studies are needed to elucidate the causal relationship.
Topics: Adult; Aged; Chronic Disease; Humans; Microbiota; Middle Aged; Nasal Mucosa; RNA, Ribosomal, 16S; Rhinitis; Sinusitis
PubMed: 35252024
DOI: 10.3389/fcimb.2022.786481 -
Cureus Jan 2022is a gram-positive anaerobic coccus found on the skin, vagina, and gut, where it acts as an opportunistic pathogen or as part of polymicrobial infections of chronic...
is a gram-positive anaerobic coccus found on the skin, vagina, and gut, where it acts as an opportunistic pathogen or as part of polymicrobial infections of chronic wounds or diabetic ulcers We present a case of a 68-year-old woman who was diagnosed with a late prosthetic hip arthroplasty infection caused by and isolated from sonication fluid cultures. Despite the fact that evidence is scarce, its role and pathogenicity in more severe infections should not be underestimated.
PubMed: 35198324
DOI: 10.7759/cureus.21419 -
Archives of Microbiology Feb 2022In this study, gas production from mixed anaerobic fermentation of rapeseed cake and sheep manure at low temperature (15.2-17.8 °C) was investigated in Qinghai rural...
In this study, gas production from mixed anaerobic fermentation of rapeseed cake and sheep manure at low temperature (15.2-17.8 °C) was investigated in Qinghai rural household biogas digesters to understand the temporal dynamics of key microbial populations involved in fermentations. Different raw material ratios resulted in significantly different effects on biogas yields and microbial community compositions over 40 days. When the dry weight ratio of sheep manure to rapeseed cake was 1:2, the highest level of cumulative gas production was observed (122.92 m·t). Firmicutes, Proteobacteria, Bacteroidetes, and Actinobacteria were the dominant bacterial phyla among the 29 digester samples (total relative abundances > 79.23%), followed by Synergistetes (4.09-10.7%). Lactobacillus was the most abundant genus in the biogas digesters with high rapeseed cake contents (average relative abundances: 14.68%), while Peptoniphilus exhibited higher abundances (12.69%) in the mixed treatments. In addition, unclassified Synergistaceae abundances (6.64%) were positively associated with biogas production variation among treatments. Bacteroides (5.74%) and Pseudomonas (5.24%) both accounted for larger proportions of communities in the digesters that used more sheep manure. Methanomicrobiales (66.55%) was the most dominant archaeal group among digesters, with Methanogenium (41.82%) and Methanoculleus (16.55%) representing the main gas-producing archaeal genera; they were more abundant in biogas digesters with higher sheep manure contents and higher rapeseed cake contents, respectively. VFAs and pH were the main factors associated with differences in microbial communities among the 29 samples. Specifically, VFA concentrations were positively correlated with Lactobacillus, Methanoculleus and Methanothrix abundances, while pH was positively correlated with Bacteroides, Pseudomonas, and Methanobacterium abundances.
Topics: Anaerobiosis; Animals; Biofuels; Bioreactors; Brassica napus; Fermentation; Manure; Methane; RNA, Ribosomal, 16S; Sheep; Temperature
PubMed: 35192067
DOI: 10.1007/s00203-022-02796-y -
Annals of Laboratory Medicine Jul 2022
Topics: Abscess; Craniofacial Abnormalities; Firmicutes; Humans
PubMed: 35177572
DOI: 10.3343/alm.2022.42.4.488 -
Microbiology Spectrum Feb 2022Cognitive impairment (CI) is among the most common non-motor symptoms of Parkinson's disease (PD), with a substantially negative impact on patient management and...
Cognitive impairment (CI) is among the most common non-motor symptoms of Parkinson's disease (PD), with a substantially negative impact on patient management and outcome. The development and progression of CI exhibits high interindividual variability, which requires better diagnostic and monitoring strategies. PD patients often display sweating disorders resulting from autonomic dysfunction, which has been associated with CI. Because the axillary microbiota is known to change with humidity level and sweat composition, we hypothesized that the axillary microbiota of PD patients shifts in association with CI progression, and thus can be used as a proxy for classification of CI stages in PD. We compared the axillary microbiota compositions of 103 PD patients (55 PD patients with dementia [PDD] and 48 PD patients with mild cognitive impairment [PD-MCI]) and 26 cognitively normal healthy controls (HC). We found that axillary microbiota profiles differentiate HC, PD-MCI, and PDD groups based on differential ranking analysis, and detected an increasing trend in the log ratio of to in progression from HC to PDD. In addition, phylogenetic factorization revealed that the depletion of the , and genera is associated with PD-MCI and PDD. Moreover, functional predictions suggested significant increases in myo-inositol degradation, ergothioneine biosynthesis, propionate biosynthesis, menaquinone biosynthesis, and the proportion of aerobic bacteria and biofilm formation capacity, in parallel to increasing CI. Our results suggest that alterations in axillary microbiota are associated with CI in PD. Thus, axillary microbiota has the potential to be exploited as a noninvasive tool in the development of novel strategies. Parkinson's disease (PD) is the second most common neurodegenerative disease. Cognitive impairment (CI) in PD has significant negative impacts on life quality of patients. The emergence and progression of cognitive impairment shows high variability among PD patients, and thus requires better diagnostic and monitoring strategies. Recent findings indicate a close link between autonomic dysfunction and cognitive impairment. Since thermoregulatory dysfunction and skin changes are among the main manifestations of autonomic dysfunction in PD, we hypothesized that alterations in the axillary microbiota may be useful for tracking cognitive impairment stages in PD. To our knowledge, this the first study characterizing the axillary microbiota of PD patients and exploring its association with cognitive impairment stages in PD. Future studies should include larger cohorts and multicenter studies to validate our results and investigate potential biological mechanisms.
Topics: Aged; Axilla; Bacteria; Cognitive Dysfunction; Female; Humans; Male; Microbiota; Middle Aged; Parkinson Disease; Phylogeny
PubMed: 35138147
DOI: 10.1128/spectrum.02358-21 -
Nan Fang Yi Ke Da Xue Xue Bao = Journal... Dec 2021To compare the composition of gut microbiome in pre-adolescent children with different meat consumption patterns.
OBJECTIVE
To compare the composition of gut microbiome in pre-adolescent children with different meat consumption patterns.
METHODS
This study was conducted among 44 healthy school-age children (age range 8-10 years) in Shenzhen. According to the monthly intake frequency ratio of white meat and red meat, the children were divided into red-meat group (=15), balanced group (=16) and white-meat group (=13). The Food Frequency Questionnaire (FFQ) was used to investigate the children's diet, and samples of morning feces were collected to study the gut microbiome. The fecal DNA was extracted and amplified, and the composition of the intestinal microbiome of the children was analyzed using Illumina Miseq high-throughput sequencing.
RESULTS
The children in red meat and white meat groups showed significantly lower abundance and diversity of gut microbiota than those with a balanced diet ( < 0.05). LEfSe analysis of the genus in the fecal samples showed that , and were enriched in red-meat group and was enriched in the white-meat group as compared with the balanced group. In the samples of the balanced group, 31 and 25 genus (such as and ) were significantly enriched as compared with the samples of the red-meat group and the white-meat group, respectively. Prediction of the gut microbiota KEGG pathway using PICRUSt2 suggested that compared with that in the balanced group, the gut microbiota in red-meat group had significant activation of the pathways involving lipopolysaccharide biosynthesis ( < 0.01), arachidonic acid metabolism ( < 0.01), thyroid hormone synthesis ( < 0.001), and carbohydrate digestion and absorption ( < 0.05). But compared with the white-meat group, the red-meat group showed only significant activation of the pathways of arachidonic acid metabolism ( < 0.05) and thyroid hormone synthesis ( < 0.05).
CONCLUSION
The preference of red meat and white meat consumption may significantly reduce the abundance and diversity of gut microbiota in pre-adolescent children. A red meat-rich diet may cause enrichment of and significant activation of lipopolysaccharide biosynthesis pathway, suggesting the potential benefit of a balanced diet for pre-adolescent children.
Topics: Adolescent; Child; Diet; Feces; Gastrointestinal Microbiome; Humans; Meat; RNA, Ribosomal, 16S
PubMed: 35012911
DOI: 10.12122/j.issn.1673-4254.2021.12.07 -
Frontiers in Cellular and Infection... 2021Gram-positive anaerobic cocci (GPAC) are a commensal part of human flora but are also opportunistic pathogens. This is possibly the first study to report a case of...
Gram-positive anaerobic cocci (GPAC) are a commensal part of human flora but are also opportunistic pathogens. This is possibly the first study to report a case of bacteremia in an abdominal aortic aneurysm (AAA) patient. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) failed to identify the isolate and molecular analysis confirmed it as . A comprehensive literature review revealed that is an emergent pathogen. This study serves as a reminder for practicing clinicians to include anaerobic blood cultures as part of their blood culture procedures; this is particularly important situations with a high level of suspicion of infection factors in some noninfectious diseases, as mentioned in this publication. Clinical microbiologists should be aware that the pathogenic potential of GPAC can be greatly underestimated leading to incorrect diagnosis on using only one method for pathogen identification. Upgradation and correction of the MALDI-TOF MS databases is recommended to provide reliable and rapid identification of GPAC at species level in medical diagnostic microbiology laboratories.
Topics: Aortic Aneurysm; Blood Culture; Firmicutes; Gram-Positive Cocci; Humans; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
PubMed: 35004343
DOI: 10.3389/fcimb.2021.755225