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A Novel Rhodamine-Phenolphthalein Architecture for Selective Mercury Ion Detection in Aqueous Media.ChemPlusChem May 2024In this study, the primary objective is to synthesize a novel fluorescent Rh-PP-Rh compound and explore its extensive range of photochemical behaviors. Initially, the...
In this study, the primary objective is to synthesize a novel fluorescent Rh-PP-Rh compound and explore its extensive range of photochemical behaviors. Initially, the synthesis of the novel Rh-PP-Rh was carried out for this purpose. Subsequently, UV-Vis and fluorescence spectroscopy were employed to investigate the interactions between Rh-PP-Rh and a diverse array of ions in aqueous solvent systems. Through fluorescence and UV-Vis studies, it was observed that Rh-PP-Rh demonstrated turn-on sensor properties in the presence of Hg ions. Furthermore, the limits of detection (LOD) and association constant (K) values for Rh-PP-Rh/Hg were determined as 334 nM and 9.13×10 M, respectively. Additionally, the reversible studies demonstrated a switchable on/off response upon alternate addition of HgCl and [BuN]F to Rh-PP-Rh. These findings suggest that the probe Rh-PP-Rh also possesses specific sensor properties for F ions in the presence of mercury. In addition, the investigation encompassed an assessment of the visual analysis of the color alterations of Rh-PP-Rh both on filter paper and in an EtOH/HO solution. The findings demonstrated that Rh-PP-Rh can be successfully utilized in solutions containing mercury, as it generates significant color transformations.
PubMed: 38179811
DOI: 10.1002/cplu.202300649 -
Spectrochimica Acta. Part A, Molecular... Mar 2024Owed to rapid responsive pH sensing attributes, the zinc-supported Silica-titania (Z/ST) nanocomposite is synthesized by the sol-gel method. Three distinct pH dyes such...
Owed to rapid responsive pH sensing attributes, the zinc-supported Silica-titania (Z/ST) nanocomposite is synthesized by the sol-gel method. Three distinct pH dyes such as phenolphthalein, creosol red, and phenol red are immobilized in Z/ST to examine their influence on the structural and optical properties of Z/ST nanocomposite. Microscopic analyses possessed that Z/ST and dyes immobilized Z/ST have crack-free porous morphology. The phenol red immobilized Z/ST (P-Z/ST) has a low surface roughness ∼0.5 nm, ultra-thin coating ∼2.1 nm, and more hydrophilic features than C-Z/ST (creosol red immobilized Z/ST), and HPh-Z/ST (phenolphthalein immobilized Z/ST). The P-Z/ST possessed 0.3 s against pH 12 and pka 10, C-Z/ST shows a time response 0.5 s and pka (negative log of the acid dissociation constant) value around 8, while Hph-Z/ST revealed a time response 0.68 s and high pka around 11. All three nanocomposites attributed stable and fast responses in basic media.
PubMed: 38061110
DOI: 10.1016/j.saa.2023.123686 -
Scientific Reports Nov 2023This paper presents comprehensive research of the advantages and applicability of various concrete carbonation detection methods. Employing a combination of...
This paper presents comprehensive research of the advantages and applicability of various concrete carbonation detection methods. Employing a combination of Phenolphthalein indicator (PI), Thermogravimetric analysis (TGA), X-ray phase analysis (XRD), Fourier transform infrared spectroscopy (FTIR), and Quantitative calcium carbonate analysis (CA), a detailed comparison to determine the carbonation depth in the partial carbonation zone of concrete specimens is conducted. Among the quantitative analysis methods, CA measures CaCO content based on chemical reactions, while TGA obtains the concentration distribution of Ca(OH) and CaCO. Among qualitative analysis methods, XRD tested the intensity distribution of Ca(OH) and CaCO, while FTIR traced the characteristic peaks of C-O functional groups in a specific spectral range to determine the depth of carbonation of concrete. Results indicate that the depth of carbonation values measured by CA, TGA, XRDA, and FTIR are 2-3 times higher than those measured by PI. This research may provide valuable insights for the design of carbonation detection in concrete.
PubMed: 37968286
DOI: 10.1038/s41598-023-47443-8 -
Pharmacy (Basel, Switzerland) Sep 2023Health concerns associated with the consumption of food supplements rise in parallel with the rise in the popularity and market availability of these products. In...
Health concerns associated with the consumption of food supplements rise in parallel with the rise in the popularity and market availability of these products. In pursuit of data related to the unauthorized presence of pharmaceuticals in food supplements, the Rapid Alert System for Food and Feed (RASFF) database was searched for the 2011-2022 period. The most "popular" pharmaceuticals for the adulteration of food supplements were phosphodiesterase-5 inhibitors (235 records); anorexics and laxatives (76), including sibutramine and its active metabolite N-didesmethyl sibutramine, phenolphthalein and 2,4-dinitrophenol; stimulants, among which 1,3-dimethylamine (97), and synephrine (53) were the most numerous; nootropic drugs (24); anabolics and prohormones (16); and cannabinoid cannabidiol (14) (pending authorization as a novel food ingredient). Over 65% of notifications of interest were classified as serious risks, and over 80% of these were alert or border rejection notifications, mainly generated as a result of official control on the market. The alarming number of RASFF notifications should be considered a public health issue, demanding clear and targeted recommendation for action for the legislature and authorities. A harmonized nutrivigilance system should be considered as a tool to detect and scrutinize the adverse health effects of food supplements, along with measures to improve their safety, quality, and testing.
PubMed: 37888499
DOI: 10.3390/pharmacy11050154 -
Journal of Pediatric Orthopedics Jan 2024Proximal thoracic curve (PTC) correction has been considered to prevent lateral shoulder imbalance in Lenke Type 2 adolescent idiopathic scoliosis (AIS) patients;...
BACKGROUND
Proximal thoracic curve (PTC) correction has been considered to prevent lateral shoulder imbalance in Lenke Type 2 adolescent idiopathic scoliosis (AIS) patients; however, postoperative shoulder imbalance (PSI) commonly occurs despite these strategies with PTC correction. We investigated the hypothesis that PTC correction would not directly affect PSI in the majority of Lenke type 2 AIS cases. Furthermore, we investigated the risk factors for lateral PSI after corrective surgery.
METHODS
This study examined the records for AIS patients with Lenke type 2 who underwent corrective surgery and followed up for >2 years. Patients were categorized into PSI (-); radiologic shoulder height (RSH)<15 mm, and PSI (+); RSH≥15 mm. Repeated measures analysis of variance was performed at preoperatively, postoperatively, 1 month, and final follow-up. Postoperative lateral shoulder imbalance was predicted by the identification of univariate analysis and multivariate analysis.
RESULTS
Among the 151 patients reviewed, 29 (19.2%) showed PSI at final follow-up. Lateral shoulder balance parameters showed different directionalities between PSI (-) and (+) groups at postoperatively, 1 month, and final follow-up ( P <0.01 each). Preoperative PTC, middle thoracic curve (MTC) curve and MTC correction showed strong correlations with the RSH ( P =0.01, 0.03, and 0.04, respectively). However, PTC correction did not show a significant correlation with the RSH. Moreover, only a smaller MTC curve and larger MTC correction rate were related to lateral PSI in multivariate analysis.
CONCLUSIONS
In Lenke type 2 AIS curves, the MTC curve and its correction predominantly influence lateral shoulder imbalance after corrective surgery, irrespective of the PTC correction extent. Consequently, overemphasizing the correction of the PTC curve may not necessarily lead to an improved lateral shoulder balance. When MTC curve is smaller, surgeons should be more careful for MCT overcorrection leading to a lateral shoulder imbalance.
LEVEL OF EVIDENCE
Level III.
Topics: Humans; Adolescent; Shoulder; Scoliosis; Thoracic Vertebrae; Retrospective Studies; Spinal Fusion; Kyphosis; Phenolphthalein; Treatment Outcome
PubMed: 37815292
DOI: 10.1097/BPO.0000000000002544 -
Biomedicines Sep 2023Dental composites, through their structural diversity, represent the biomaterials frequently used in dental reconstructive therapy. The aim of our study was to observe...
Dental composites, through their structural diversity, represent the biomaterials frequently used in dental reconstructive therapy. The aim of our study was to observe the influence of different beverage environment conditions on seven types of obturation dental materials with different compositions. Our research focused on the surface modification analysis of the materials after the immersion in the different beverages; in this regard, we used the EDAX technique correlated with the energy-dispersive X-ray fluorescence (XRF). The pH of the drinks and that of the simulated saliva solution were determined by the titrimetric method, a sodium hydroxide solution 0.1 mol/dm was prepared and used for the titration. An amount of 5 mL of each analyzed solution was added to 15 mL of distilled water to obtain a dilution, to which 3 drops of phenolphthalein (as a color indicator-Phenolphthalein, 3,3-Bis(4-hydroxyphenyl)-1(3H)-isobenzofuranone, CHO Mw: 318.32, purchased from Merck) were added for each analysis. For each solution, the experiment was repeated three times in order to obtain accurate results. The results of our study materialized into a real plea for modifying the patients' behavior in terms of diet and preferences for acidic drinks, so that their quality-of-life valence can be improved by keeping the composite materials in a long-term unalterable state on the one hand; on the other hand, systemic damage can be prevented as well.
PubMed: 37761013
DOI: 10.3390/biomedicines11092571 -
Problemy Sotsial'noi Gigieny,... Aug 2023One of the directions to increase the effectiveness of medical organizations in the region is the development of managerial tools for improving the quality of medical...
One of the directions to increase the effectiveness of medical organizations in the region is the development of managerial tools for improving the quality of medical services. The aim of the study is to develop an approach to improving the quality of medical services based on the results of measuring patient satisfaction with the quality of medical services, mapping satisfaction criteria to medical and technological characteristics of the quality of services, substantiation and selection of appropriate corrective measures. The method of measuring patient satisfaction with the quality of the provided medical service and the method of deploying quality functions ensuring transformation of patient needs and expectations into characteristics of service quality were used. The measurement of patients' satisfaction with the quality of medical services is carried out during medical and sociological research. The measurement results are obtained by statistical processing using a software set. To determine measures to improve the quality of cardiological services, the results of changing patient satisfaction were used in accordance with the method of deploying quality functions, which consists in implementing a number of steps that ensure the determination of a minimum set of additional requirements for medical services in order to meet the requirements of patients. This technique is supported by specialized software. Implementation of the proposed approach makes it possible to form tools aimed at creating an information model of medical services and ensure the change in the quality of such services demanded by patients.
Topics: Humans; Patient Satisfaction; Dioctyl Sulfosuccinic Acid; Phenolphthalein; Technology
PubMed: 37742263
DOI: 10.32687/0869-866X-2023-31-s1-857-861 -
Journal of Orthopaedic Surgery and... Sep 2023The severe rigid scoliosis patients with type I respiratory failure could not tolerate complicated corrective surgery. Preoperative halo-pelvic distraction (HPD) is used...
BACKGROUND
The severe rigid scoliosis patients with type I respiratory failure could not tolerate complicated corrective surgery. Preoperative halo-pelvic distraction (HPD) is used to reduce the curve magnitude and improve the pulmonary function before surgery. The present study aimed to retrospectively analyze the pulmonary and clinical outcomes of preoperative HPD in severe rigid spinal deformity with type I respiratory failure.
METHODS
Eighteen cases of severe rigid scoliosis and type I respiratory failure treated with preoperative HPD and corrective surgery for spinal deformity between 2016 and 2018 were retrospectively reviewed. Patient demographics, major coronal curve and kyphosis, correction rates, heights, pulmonary function, distraction time, and postoperative neurological complications were recorded for all cases.
RESULTS
The averaged duration of distraction was 9.1 ± 2.3 months. The coronal curve was corrected from 168° ± 14° to 58° ± 11° at the end of HPD. The kyphosis curve reduced from 151° ± 29° to 65° ± 10°. Meanwhile, the mean stand body height increased by 23.9 ± 5.3 cm. Significantly increased mean FVC (1.52 ± 0.43 L vs. 0.95 ± 0.44 L) and improved percent-predicted values for FVC (37 ± 10% vs. 23 ± 9%) were observed after HPD. The pressure of oxygen (PaO2) increased from 54.5 ± 2.0 to 84.8 ± 4.7 mmHg. Scoliosis and kyphosis curve, respectively, averaged 48 ± 8°and 30 ± 14° after final fusion and instrumentation, with a mean correction of 71% and 80%, respectively. No severe complication occurred during the distraction.
CONCLUSIONS
HPD may be useful for severe rigid scoliosis patients with type I respiratory failure. Pulmonary functions in patients with severe rigid scoliosis can be significantly improved by HPD. They are then better able to tolerate complicated corrective surgery.
Topics: Humans; Retrospective Studies; Scoliosis; Oxygen; Body Height; Dioctyl Sulfosuccinic Acid; Kyphosis; Phenolphthalein; Postoperative Complications
PubMed: 37735661
DOI: 10.1186/s13018-023-04212-7 -
Se Pu = Chinese Journal of... Sep 2023Electrophoresis titration (ET) based on the moving reaction boundary (MRB) theory can detect the analyte contents in different samples by converting content signals into...
Electrophoresis titration (ET) based on the moving reaction boundary (MRB) theory can detect the analyte contents in different samples by converting content signals into distance signals. However, this technique is only suitable for on-site qualitative testing, and accurate quantification relies on complex optical equipment and computers. Hence, applying this method to real-time point-of-care testing (POCT) is challenging. In this study, we developed a smartphone-based ET system based on a visual technique to achieve real-time quantitative detection. First, we developed a portable quantitative ET device that can connect to a smartphone; this device consisted of five components, namely, an ET chip, a power module, a microcontroller, a liquid crystal display screen, and a Bluetooth module. The device measured 10 cm×15 cm×2.5 cm, weighed 300 g, and was easy to hold. Thus, it is suitable for on-site testing with a run time of only 2-4 min. An assistant mobile software program was also developed to control the device and perform ET. The colored electrophoresis boundary can be captured using the smartphone camera, and quantitative detection results can be obtained in real time. Second, we proposed a quantitative algorithm based on ET channels. The software was used to recognize the boundary migration distance of three channels, a standard curve based on two given contents of the standards was established using the two-point method, and the content of the test sample was calculated. Human serum albumin (HSA) and uric acid (UA) were used as a model protein and biosample, respectively, to test the performance of the detection system. For HSA detection, different HSA solutions were mixed with a polyacrylamide gel (PAG) stock solution, phenolphthalein was added as an indicator, and sodium persulfate and tetramethyl ethylenediamine (TEMED) were used to promote polymerization to form a gel. For UA detection, agarose gel was filled into the ET channel, the UA sample, urate oxidase, and leucomalachite green were added into the anode cell and incubated for 20 min. ET was then performed. The fitting goodness () values of HSA and UA were 0.9959 and 0.9935, respectively, with a linear range of 0.5-35.0 g/L and a log-linear range of 100-4000 μmol/L. The limits of detection for HSA and UA were 0.05 g/L and 50 μmol/L, respectively, and the corresponding relative standard deviations (RSDs) were not greater than 2.87% and 3.21%, respectively. These results demonstrate that the detection system has good accuracy and sensitivity. Clinical samples collected from healthy volunteers were used as target blood samples, and the developed system was used to measure serum total protein and UA levels. Serum samples from five volunteers were selected, standard curves of total serum protein and UA were established, and the test results were compared with hospital standard testing results. The relative errors for serum total protein and UA were less than 6.03% and 6.21%, respectively, and the corresponding RSDs were less than 3.72% and 5.84%, respectively. These findings verify the accuracy and reliability of the proposed detection system. The smartphone-based ET detection system introduced in this paper presents several advantages. First, it enables the portable real-time detection of total serum protein and UA. Second, compared with traditional ET strategies based on colored boundaries, it does not rely on optical detection equipment or computers to obtain quantitative detection results; as such, it can reduce the complexity of the operation and provide portability and real-time metrics. Third, the detection of two biomarkers, serum total protein and UA, is achieved on the same device, thereby improving the multitarget detection potential of the ET method. These advantages render the developed method a promising detection platform for clinical applications and real-time POCT.
Topics: Humans; Smartphone; Reproducibility of Results; Electrophoresis; Electrodes; Blood Proteins
PubMed: 37712539
DOI: 10.3724/SP.J.1123.2023.06001 -
Environmental Toxicology and Chemistry Dec 2023The US Environmental Protection Agency (USEPA) is faced with long lists of chemicals that require hazard assessment. The present study is part of a larger effort to...
The US Environmental Protection Agency (USEPA) is faced with long lists of chemicals that require hazard assessment. The present study is part of a larger effort to develop in vitro assays and quantitative structure-activity relationships applicable to untested chemicals on USEPA inventories through study of estrogen receptor (ER) binding and estrogen-mediated gene expression in fish. The present effort investigates metabolic activation of chemicals resulting in increased estrogenicity. Phenolphthalin (PLIN) was shown not to bind rainbow trout (Oncorhynchus mykiss) ER (rtER) in a competitive binding assay, but vitellogenin (Vtg) expression was induced in trout liver slices exposed to 10 and 10 M PLIN. Phenolphthalein (PLEIN), a metabolite of PLIN, was subsequently determined to be formed when slices were exposed to PLIN. It binds rtER with a relative binding affinity to 17β-estradiol of 0.020%. Slices exposed to PLEIN expressed Vtg messenger RNA (mRNA) at 10 , 10 , and 10 M, with no detectable PLIN present. Thus, Vtg expression noted in PLIN slice exposures was explained by metabolism to PLEIN in trout liver slices. A second model chemical, 4,4'-methylenedianiline (MDA), was not shown to bind rtER but did induce Vtg mRNA production in tissue slices at 10 , 10 , and 10 M in amounts nearly equal to reference estradiol induction, thus indicating metabolic activation of MDA. A series of experiments were performed to identify a potential metabolite responsible for the observed increase in activity. Potential metabolites hydroxylamine-MDA, nitroso-MDA, azo-MDA, and azoxy-MDA were not observed. However, acetylated MDA was observed and tested in both ER-binding and tissue slice Vtg induction assays. Environ Toxicol Chem 2023;42:2747-2757. © 2023 SETAC. This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA.
Topics: Humans; Animals; Activation, Metabolic; Xenobiotics; Estradiol; Vitellogenins; Oncorhynchus mykiss; RNA, Messenger
PubMed: 37712519
DOI: 10.1002/etc.5748