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Gastrointestinal Endoscopy Nov 2015There are few data comparing endoscopic treatment outcomes for Barrett's esophagus (BE). (Observational Study)
Observational Study
BACKGROUND
There are few data comparing endoscopic treatment outcomes for Barrett's esophagus (BE).
OBJECTIVE
To compare treatment outcomes in BE patients treated with radiofrequency ablation (RFA), RFA after EMR, and porfimer sodium photodynamic therapy (Ps-PDT).
DESIGN
Retrospective, observational study.
SETTING
Single tertiary center between 2001 and 2013.
PATIENTS
A total of 342 BE patients treated with RFA (n = 119), EMR+RFA (n = 98), and Ps-PDT (n = 125).
MAIN OUTCOME MEASUREMENTS
Rates of complete remission of intestinal metaplasia (CRIM), BE recurrence, and adverse events.
RESULTS
Baseline BE high-grade dysplasia (HGD) and adenocarcinoma were more common in the Ps-PDT group (89%) compared with the EMR-RFA (70%) and RFA (37%) groups. At a median follow-up of 14.2 months, 173 patients (50.6%) achieved CRIM. CRIM was significantly more common in Ps-PDT patients compared with RFA (P < .001) and EMR-RFA (P < .001) patients on multivariable analysis. In patients who achieved CRIM, the rates of subsequent BE recurrence were relatively similar among the 3 groups. Although the rates of bleeding were similar, strictures were less common in RFA patients (2.4%) compared with EMR-RFA (13.3%, P = .001) and Ps-PDT (10.4%, P =.043) patients.
CONCLUSION
This study of endoscopic treatment for Barrett's dysplasia and neoplasia found that complete remission was achieved more often and more rapidly after Ps-PDT with similar disease recurrence rates compared with EMR or RFA. Adverse events were more common after EMR and Ps-PDT. Further studies are required to determine which ablation and resection techniques are ideally suited for each BE patient.
Topics: Adult; Aged; Aged, 80 and over; Barrett Esophagus; Catheter Ablation; Dihematoporphyrin Ether; Esophagoscopy; Esophagus; Female; Humans; Male; Metaplasia; Middle Aged; Photochemotherapy; Photosensitizing Agents; Retrospective Studies; Time Factors; Treatment Outcome
PubMed: 26071064
DOI: 10.1016/j.gie.2015.03.1979 -
Biomedicine & Pharmacotherapy =... Apr 2015Ovarian cancer is among the most lethal cancers in women. The successful anticancer treatment depends on the effectiveness of cytotoxic effect of applied therapeutic...
Oxidative modification induced by photodynamic therapy with Photofrin®II and 2-methoxyestradiol in human ovarian clear carcinoma (OvBH-1) and human breast adenocarcinoma (MCF-7) cells.
Ovarian cancer is among the most lethal cancers in women. The successful anticancer treatment depends on the effectiveness of cytotoxic effect of applied therapeutic procedures either alone or in combination with other treatments. Photodynamic therapy (PDT) is a relatively new method of anticancer therapy. Its dominant mechanism of action is the over-production of reactive oxygen species induced by oxidative stress in malignant cells, which attack lipid membranes, proteins and nucleic acids. One of the important mechanisms is induction of unfolded protein response, ubiquitin-proteasome pathway of protein degradation. The aim of this study was to evaluate the cytotoxic effect of various protective enzymes in ovarian carcinoma clear cell line in comparison to the model breast cell line after photodynamic reaction and photodynamic reaction with 2-methoxyestradiol (2-Me). Human malignant ovarian cell line (OvBH-1) was used and human breast adenocarcinoma cells (MCF-7) were used as a control. Photodynamic reaction (PDR) with Photofrin(®)II and Ph(®)II with 2-Me was performed. The expression of protective proteins by immunocytochemistry (HSP70 and iNOS) and western blot (Hsp27 and Hsp70) methods was evaluated directly, 3 and 6 h after PDR. The changes in cells' cytoskeleton were evaluated using immunofluorescence by confocal microscopy. The expression of iNOS was observed for both experiments with differential intensity and quantity. A higher expression of Hsp70 in MCF-7 cells was observed than in OvBh-1 cells. The reorganization of cytoskeleton and nucleus was observed after 3 and 6 h after exposition to light.
Topics: 2-Methoxyestradiol; Adenocarcinoma; Blotting, Western; Breast Neoplasms; Cell Line, Tumor; Cytoskeleton; Dihematoporphyrin Ether; Estradiol; Female; HSP27 Heat-Shock Proteins; HSP70 Heat-Shock Proteins; Humans; Immunohistochemistry; Nitric Oxide Synthase Type II; Ovarian Neoplasms; Oxidation-Reduction; Photochemotherapy
PubMed: 25960212
DOI: 10.1016/j.biopha.2015.02.008 -
Gastrointestinal Endoscopy Apr 2015
Topics: Bile Duct Neoplasms; Bile Ducts, Intrahepatic; Cholangiocarcinoma; Dihematoporphyrin Ether; Female; Humans; Lasers, Semiconductor; Male; Photosensitizing Agents
PubMed: 25805484
DOI: 10.1016/j.gie.2014.12.046 -
Gastrointestinal Endoscopy Apr 2015
Topics: Bile Duct Neoplasms; Bile Ducts, Intrahepatic; Cholangiocarcinoma; Dihematoporphyrin Ether; Female; Humans; Lasers, Semiconductor; Male; Photosensitizing Agents
PubMed: 25805483
DOI: 10.1016/j.gie.2014.10.039 -
Cellular Physiology and Biochemistry :... 2015Photodynamic therapy (PDT) is a promising noninvasive technique, which has been successfully applied to the treatment of human cancers. Studies have shown that the Bcl-2...
BACKGROUND/AIMS
Photodynamic therapy (PDT) is a promising noninvasive technique, which has been successfully applied to the treatment of human cancers. Studies have shown that the Bcl-2 family proteins play important roles in PDT-induced apoptosis. However, whether Bcl-2-interacting mediator of cell death (Bim) is involved in photodynamic treatment remains unknown. In this study, we attempt to determine the effect of Bim on Photofrin photodynamic treatment (PPT)-induced apoptosis in human lung adenocarcinoma ASTC-a-1 cells.
METHODS
The translocation of Bim/Bax of the cells were monitored by laser confocal scanning microscope. The levels of Bim protein and activated caspase-3 in cells were detected by western blot assay. Caspase-3 activities were measured by Caspase-3 Fluorogenic Substrate (Ac-DEVD-AFC) analysis. The induction of apoptosis was detected by Hoechst 33258 and PI staining as well as flow cytometry analysis. The effect of Bim on PPT-induced apoptosis was determined by RNAi.
RESULTS
BimL translocated to mitochondria in response to PPT, similar to the downstream pro-apoptotic protein Bax activation. PPT increased the level of Bim and activated caspase-3 in cells and that knockdown of Bim by RNAi significantly protected against caspase-3 activity. PPT-induced apoptosis were suppressed in cells transfected with shRNA-Bim.
CONCLUSION
We demonstrated the involvement of Bim in PPT-induced apoptosis in human ASTC-a-1 lung adenocarcinoma cells and suggested that enhancing Bim activity might be a potential strategy for treating human cancers.
Topics: Apoptosis; Apoptosis Regulatory Proteins; Bcl-2-Like Protein 11; Blotting, Western; Caspase 3; Cell Line, Tumor; Dihematoporphyrin Ether; Humans; Lasers; Membrane Proteins; Microscopy, Electron, Scanning; Mitochondria; Photochemotherapy; Photosensitizing Agents; Proto-Oncogene Proteins; RNA Interference; RNA, Small Interfering; bcl-2-Associated X Protein
PubMed: 25791936
DOI: 10.1159/000373968 -
Melanoma Research Jun 2015Photodynamic therapy has been considered ineffective for melanomas because of the competition between the absorbance of melanin from the melanoma and the absorbance of... (Comparative Study)
Comparative Study
Photodynamic therapy has been considered ineffective for melanomas because of the competition between the absorbance of melanin from the melanoma and the absorbance of photosensitizers at the photosensitizer excitation light wavelength. Melanomas show considerable heterogeneity and resistance to phototherapy. The effectiveness of photodynamic therapy could be intensified by electroporation for enhanced transport of a photosensitizer by transient pores in the membrane. In this study, photodynamic therapy combined with electroporation was tested in vitro on the human melanoma cell lines melanotic melanoma (MeWo) and amelanotic melanoma (C32). Control experiments were conducted on human keratinocytes (HaCaT). Photofrin was used as a photosensitizer. Photosensitizer distribution, cloning efficacy test, comet assay, and assessment of apoptotic proteins were performed. Melanin levels were determined before and after photodynamic therapy. The experiments indicated that electroporation effectively supports the photodynamic method. It was found that photodynamic therapy with electroporation efficiently induces apoptosis in melanotic and amelanotic melanoma cells.
Topics: Absorption, Physiological; Antineoplastic Agents; Apoptosis; Caspases; Cell Line, Transformed; Cell Line, Tumor; Comet Assay; DNA Damage; Dihematoporphyrin Ether; Electrochemotherapy; Electroporation; Humans; Keratinocytes; Melanins; Melanoma; Melanoma, Amelanotic; Neoplasm Proteins; Photochemotherapy; Photosensitizing Agents; Time Factors
PubMed: 25756554
DOI: 10.1097/CMR.0000000000000153 -
Gan To Kagaku Ryoho. Cancer &... Nov 2014In case of central type lung cancer, it happens that tumors tend to grow at many foci of bronchial tree, sometimes simultaneously and sometimes not. And these patients...
In case of central type lung cancer, it happens that tumors tend to grow at many foci of bronchial tree, sometimes simultaneously and sometimes not. And these patients with such abnormalities often suffer from pulmonary emphysema or chronic bronchitis because of heavy smoking habits. So it is important to choose the treatment which preserve the pulmonary function in these cases. Today among several kinds of treatments, photodynamic therapy (PDT) is the definite method to maintain lung function. We report here a case of multiple central type lung cancer treated successfully by PDT.
Topics: Carcinoma, Squamous Cell; Dihematoporphyrin Ether; Humans; Lung Neoplasms; Male; Middle Aged; Neoplasms, Multiple Primary; Photochemotherapy; Photosensitizing Agents; Radiography
PubMed: 25731416
DOI: No ID Found -
International Journal of Molecular... Feb 2015Photodynamic therapy (PDT) is an effective local treatment modality as a cancer-specific laser ablation in malignancy of some organs including digestive tracts or bile...
Photodynamic therapy (PDT) is an effective local treatment modality as a cancer-specific laser ablation in malignancy of some organs including digestive tracts or bile duct. In Japan, PDT has been applied at the early period after the first clinical induction in 1980's. Although the useful efficacy was clarified, PDT has not been fully applied because of the phototoxicity of the porfimer sodium. The next generated talaporfin-sodium was used for PDT, in which phototoxicity was reduced and, however, the clinical efficacy for digestive tract malignancy has not yet been clarified. By proceeding the experimental and clinical trials, it is necessary to clarify the evidence of efficacy as a local powerful treatment with the conventional surgery, brachiotherapy and chemotherapy in the future step.
Topics: Carcinoma; Dihematoporphyrin Ether; Endoscopy, Gastrointestinal; Gastrointestinal Neoplasms; Humans; Japan; Lasers; Photochemotherapy; Photosensitizing Agents; Porphyrins
PubMed: 25690028
DOI: 10.3390/ijms16023434 -
Journal of Biophotonics Sep 2015Despite initially positive responses, recurrences after Photodynamic treatment (PDT) can occur and there is need for improvement in the effectiveness of PDT. Our study...
Despite initially positive responses, recurrences after Photodynamic treatment (PDT) can occur and there is need for improvement in the effectiveness of PDT. Our study uniquely showed that there was a significantly gap junctional intercellular communication (GJIC)-dependent PDT cytotoxicity. The presence of GJIC composed of Connexin 32 increased the PDT phototoxicity in transfected HeLa cells and in the xenograft tumors, and the enhanced phototoxicity of Photofrin-mediated PDT by GJIC was related with ROS and calcium pathways. Our study indicates the possibility that up-regulation or maintenance of gap junction functionality may be used to increase the efficacy of PDT. The phototoxicity effect of Photofrin was substantially greater in Dox-treated cells, which expressed the Cx32 and formed the GJ, than Dox-untreated.
Topics: Biological Transport; Calcium; Calcium Signaling; Cell Communication; Cell Proliferation; Dihematoporphyrin Ether; Gap Junctions; HeLa Cells; Humans; Intracellular Space; Photochemotherapy; Photosensitizing Agents; Reactive Oxygen Species
PubMed: 25597481
DOI: 10.1002/jbio.201400131 -
Gastrointestinal Endoscopy Apr 2015
Topics: Bile Duct Neoplasms; Cholangiocarcinoma; Cholangiopancreatography, Endoscopic Retrograde; Dihematoporphyrin Ether; Humans; Male; Middle Aged; Palliative Care; Photochemotherapy; Photosensitizing Agents
PubMed: 25484323
DOI: 10.1016/j.gie.2014.09.060