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Journal of Endodontics Oct 2009Culture-independent studies have revealed a broad spectrum of oral bacterial taxa that may be associated with disease. This study investigated the prevalence of three...
Prevalence of new candidate pathogens Prevotella baroniae, Prevotella multisaccharivorax and as-yet-uncultivated Bacteroidetes clone X083 in primary endodontic infections.
INTRODUCTION
Culture-independent studies have revealed a broad spectrum of oral bacterial taxa that may be associated with disease. This study investigated the prevalence of three new candidate oral pathogens: Prevotella baroniae, Prevotella multisaccharivorax, and as-yet-uncultivated Bacteroidetes oral clone X083 in primary endodontic infections using a devised culture-independent approach.
METHODS
Genomic DNA was isolated from samples taken from 52 teeth with different forms of apical periodontitis and used as template in a taxon-specific 16S rRNA gene-based nested polymerase chain reaction assay to determine the prevalence of the 3 target taxa.
RESULTS
Bacteroidetes clone X083, P. baroniae, and P. multisaccharivorax were respectively detected in 81%, 43%, and 38% of the root canals of teeth associated with chronic apical periodontitis, in 60%, 40% and 40% of the canals of teeth with acute apical periodontitis, and in 14%, 24%, and 5% of the pus aspirates from acute apical abscesses. No targeted taxon was positively associated with abscesses or clinical symptoms. Overall, Bacteroidetes clone X083, P. baroniae, and P. multisaccharivorax were found in 50%, 35%, and 25% of the samples taken from primary endodontic infections, respectively.
CONCLUSIONS
Findings confirmed that the two newly named species and the uncultivated phylotype targeted in this study are associated with different forms of apical periodontitis, and a pathogenetic or at least an ecologic role is suspected.
Topics: Acute Disease; Adolescent; Adult; Aged; Bacterial Infections; Bacteroidaceae Infections; Bacteroidetes; Chronic Disease; DNA, Bacterial; Dental Pulp Cavity; Dental Pulp Diseases; Genome, Bacterial; Humans; Middle Aged; Periapical Abscess; Periapical Periodontitis; Prevotella; RNA, Bacterial; RNA, Ribosomal, 16S; Reverse Transcriptase Polymerase Chain Reaction; Young Adult
PubMed: 19801230
DOI: 10.1016/j.joen.2009.05.033 -
Journal of Dental Research Jan 2009As the breadth of bacterial diversity in the oral cavity has been deciphered by molecular studies, several newly identified species/phylotypes have emerged as potential...
As the breadth of bacterial diversity in the oral cavity has been deciphered by molecular studies, several newly identified species/phylotypes have emerged as potential pathogens. We hypothesized that many of these species/phylotypes could also be involved with the etiology of endodontic abscesses. Abscess aspirates from 42 persons were analyzed for the presence of 81 species/phylotypes by means of a reverse-capture checkerboard hybridization assay. Associations between the most frequently detected taxa were calculated. The most prevalent taxa were Fusobacterium nucleatum, Parvimonas micra, and Porphyromonas endodontalis. Other frequently found taxa included Olsenella uli, streptococci, Eikenella corrodens, some as-yet-uncultivated phylotypes (Bacteroidetes clone X083 and Synergistes clone BA121), and newly named species (Prevotella baroniae and Dialister invisus). Several positive bacterial associations were disclosed. Findings not only strengthen the association of many cultivable species with abscesses, but also include some newly named species and uncultivated phylotypes in the set of candidate pathogens associated with this disease.
Topics: Actinobacteria; Acute Disease; Adult; Bacteria; Bacteria, Anaerobic; Bacteroides; Bacteroidetes; DNA, Bacterial; Eikenella corrodens; Fusobacterium nucleatum; Gram-Negative Anaerobic Straight, Curved, and Helical Rods; Humans; Nucleic Acid Hybridization; Peptostreptococcus; Periapical Abscess; Polymerase Chain Reaction; Porphyromonas endodontalis; Prevotella; RNA, Bacterial; RNA, Ribosomal, 16S; Selenomonas; Streptococcus; Treponema denticola
PubMed: 19131319
DOI: 10.1177/0022034508328124 -
International Journal of Systematic and... Mar 2006Eight strains of anaerobic Gram-negative bacilli isolated from infections of the skin and soft tissues were subjected to a comprehensive range of phenotypic and...
Eight strains of anaerobic Gram-negative bacilli isolated from infections of the skin and soft tissues were subjected to a comprehensive range of phenotypic and genotypic tests. 16S rRNA gene sequence analysis revealed the strains to constitute a homogeneous group, distinct from species with validly published names but related to a cluster including Prevotella buccae, Prevotella dentalis and Prevotella baroniae. A novel species, Prevotella bergensis sp. nov., is proposed to accommodate these strains. Prevotella bergensis is saccharolytic and produces acetic and succinic acids as end products of fermentation. The G + C content of the DNA of the type strain is 48 mol%. The type strain of Prevotella bergensis is 94067913T (= DSM 17361T = CCUG 51224T).
Topics: DNA, Ribosomal; Fatty Acids; Humans; Nucleic Acid Hybridization; Phenotype; Phylogeny; Prevotella; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Skin Diseases, Bacterial; Soft Tissue Infections
PubMed: 16514036
DOI: 10.1099/ijs.0.63888-0 -
International Journal of Systematic and... Jul 2005Eleven strains of anaerobic Gram-negative bacilli isolated from the human oral cavity were subjected to a comprehensive range of phenotypic and genotypic tests and were...
Eleven strains of anaerobic Gram-negative bacilli isolated from the human oral cavity were subjected to a comprehensive range of phenotypic and genotypic tests and were found to comprise two homogeneous groups, designated E2 and E4. 16S rRNA gene sequence analysis revealed that members of both groups belonged to the genus Prevotella but were distinct from any species with validly published names. This distinction was confirmed by DNA-DNA hybridization and phenotypic tests. Two novel species are therefore proposed: Prevotella marshii sp. nov. (group E2) and Prevotella baroniae (group E4). Both species are saccharolytic; the end-products of fermentation for P. marshii are acetic, propionic and succinic acids, while P. baroniae produces acetic and succinic acids with minor amounts of isovaleric and isobutyric acids. The G+C content of the DNA of the type strain of Prevotella marshii is 51 mol% and that of Prevotella baroniae is 52 mol%. The type strain for P. marshii is E9.34(T) (=DSM 16973(T)=CCUG 50419(T)) and that for P. baroniae is E9.33(T) (=DSM 16972(T)=CCUG 50418(T)).
Topics: Bacterial Typing Techniques; Bacteroidaceae Infections; DNA, Bacterial; DNA, Ribosomal; Genes, rRNA; Humans; Molecular Sequence Data; Mouth; Nucleic Acid Hybridization; Phenotype; Phylogeny; Prevotella; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Species Specificity
PubMed: 16014480
DOI: 10.1099/ijs.0.63634-0