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International Journal of Systematic and... Apr 2007Gram-negative anaerobic rods were isolated from a human breast abscess. Based on genotypic and phenotypic characteristics, the novel strain belonged to the genus...
Gram-negative anaerobic rods were isolated from a human breast abscess. Based on genotypic and phenotypic characteristics, the novel strain belonged to the genus Prevotella. Phylogenetic analysis based on 16S rRNA gene sequence comparisons showed that it was closely related to Prevotella buccalis (94 % 16S rRNA gene sequence similarity), Prevotella salivae (90 %) and Prevotella oris (89.1 %). The major cellular fatty acid was C(14 : 0) (19.5 %). The new isolate represents a novel species in the genus Prevotella, for which the name Prevotella timonensis sp. nov. is proposed. The type strain is strain 4401737(T) (=CIP 108522(T)=CCUG 50105(T)).
Topics: Abscess; Adult; Bacteroidaceae Infections; Breast; DNA, Bacterial; DNA, Ribosomal; Female; Humans; Molecular Sequence Data; Phylogeny; Prevotella; RNA, Ribosomal, 16S
PubMed: 17392225
DOI: 10.1099/ijs.0.64609-0 -
Oral Surgery, Oral Medicine, Oral... May 2007To determine the bacterial species associated with spreading odontogenic infections (SOIs).
OBJECTIVE
To determine the bacterial species associated with spreading odontogenic infections (SOIs).
STUDY DESIGN
Pus samples from 4 cases of SOI were analyzed by microbiological culture methods for the presence of bacteria, and by polymerase chain reaction (PCR) amplification, cloning, and sequencing of bacterial 16S rRNA genes.
RESULTS
Culture methods identified species from the genera Prevotella, Streptococcus, and Fusobacterium, as well as anaerobic streptococci. Molecular detection methods identified a far more diverse microflora. The predominant genus detected was Prevotella, representing 102 (50.2%) of 203 clones analyzed. Prevotella oris was the most abundant species identified, representing 45 (22.2%) of 203 clones analyzed. Twelve clones (5.9%) represented uncultivable species, namely Prevotella PUS9.180, an uncultured Peptostreptococcus species, and an uncultured bacterium belonging to the Bacteroidetes phylum.
CONCLUSIONS
Prevotella species may play an important role in SOIs, and further work to examine in more detail the pathogenicity determinants of these organisms and associated host responses is warranted.
Topics: Adolescent; Adult; Bacterial Typing Techniques; Colony Count, Microbial; DNA, Bacterial; Female; Focal Infection, Dental; Gram-Negative Anaerobic Straight, Curved, and Helical Rods; Humans; Male; Polymerase Chain Reaction; Porphyromonas; Prevotella; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Suppuration
PubMed: 17141534
DOI: 10.1016/j.tripleo.2006.08.009 -
Research in Microbiology Dec 2005We report a bacterial isolate (Marseille isolate) recovered from the blood of a patient hospitalized in an intensive care unit, presenting with severe trauma, fever and...
We report a bacterial isolate (Marseille isolate) recovered from the blood of a patient hospitalized in an intensive care unit, presenting with severe trauma, fever and mechanical ventilation. Colonies appeared at 37 degrees C on blood agar after 72 h incubation. This isolate was a strictly anaerobic, Gram-negative rod phenotypically related to other Prevotella species described to date: non-motile, catalase-negative, oxidase-positive, non-glucose fermenting, resistant to vancomycin and susceptible to kanamycin. Cells exhibited a trilamellar membrane under electron microscopy. The fatty acid methyl ester profile was marginally related to that of Clostridium botulinum group A (distance: 26.27%) and Bifidobacterium bifidum GC subgroup B (distance: 26.38%). 16S rRNA gene sequence similarity was 90.0% with that of Prevotella oris and 89.1% with that of Prevotella melaninogenica. Partial rpoB gene sequence similarity was 84.5 and 86.4% with P. oris and P. melaninogenica, respectively. According to current standards, phenotypic traits, 16S rRNA and rpoB gene sequence analyses indicated that the Marseille isolate belonged to a previously unrecognized species of the genus Prevotella, and we propose classifying it in the new taxon "Prevotella massiliensis" sp. nov.
Topics: Adult; Bacteremia; Bacterial Proteins; Bacterial Typing Techniques; Bacteroidaceae Infections; Blood; Culture Media; DNA, Bacterial; DNA, Ribosomal; Genes, rRNA; Humans; Male; Molecular Sequence Data; Phenotype; Phylogeny; Prevotella; RNA, Ribosomal, 16S; Sequence Analysis, DNA
PubMed: 16085394
DOI: 10.1016/j.resmic.2005.05.008 -
International Journal of Systematic and... May 2004Two bacterial strains, EHS11(T) and EPSA11(T), which were isolated from the human oral cavity, were characterized in terms of phenotypic and biochemical characteristics,... (Comparative Study)
Comparative Study
Two bacterial strains, EHS11(T) and EPSA11(T), which were isolated from the human oral cavity, were characterized in terms of phenotypic and biochemical characteristics, cellular fatty acid profiles and phylogenetic position based on 16S rRNA gene sequence analysis. 16S rRNA gene sequence analysis showed that each of the isolates belonged to a novel species of the genus Prevotella. Strain EHS11(T) was related to Prevotella loescheii (about 95 % similarity), whereas strain EPSA11(T) was related to Prevotella oris (about 94 % similarity). Both strains were obligately anaerobic, non-pigmented, non-spore-forming, non-motile, Gram-negative rods. The cellular fatty acid composition of strain EPSA11(T) was very similar to that of P. oris JCM 8540(T). On the other hand, the cellular fatty acid composition of strain EHS11(T) was significantly different from those of other Prevotella species. The predominant fatty acids in strain EHS11(T) are C(18 : 1)omega9c, C(16 : 0) and C(16 : 0) 3-OH, whereas other Prevotella species, except for P. loescheii JCM 8530(T), possess anteiso-C(15 : 0), iso-C(17 : 0) 3-OH and C(18 : 1)omega9c. The predominant fatty acids in P. loescheii JCM 8530(T) are anteiso-C(15 : 0), C(16 : 0) and C(18 : 1)omega9c. DNA-DNA hybridization experiments revealed a genomic distinction of strains EHS11(T) and EPSA11(T) from P. loescheii JCM 8530(T) and P. oris JCM 8540(T). On the basis of these data, two novel Prevotella species are proposed: Prevotella shahii sp. nov. and Prevotella salivae sp. nov. The type strains of P. shahii and P. salivae are EHS11(T) (=JCM 12083(T)=DSM 15611(T)) and EPSA11(T) (=JCM 12084(T)=DSM 15606(T)), respectively.
Topics: DNA, Bacterial; DNA, Ribosomal; Fatty Acids; Humans; Molecular Sequence Data; Mouth; Phenotype; Phylogeny; Prevotella; RNA, Bacterial; RNA, Ribosomal, 16S; Species Specificity
PubMed: 15143039
DOI: 10.1099/ijs.0.02876-0 -
Intensive Care Medicine Aug 2004
Topics: Abscess; Bacteroidaceae Infections; Cervical Vertebrae; Gram-Positive Bacterial Infections; Humans; Male; Middle Aged; Peptostreptococcus; Postoperative Complications; Prevotella intermedia; Spinal Diseases; Tomography, X-Ray Computed
PubMed: 15034651
DOI: 10.1007/s00134-004-2265-x -
Operative Dentistry 2003Self-etching primers are now considered the new generation of dentin bonding systems that modify and incorporate the bacteria-containing smear layer into their bonding... (Comparative Study)
Comparative Study
Self-etching primers are now considered the new generation of dentin bonding systems that modify and incorporate the bacteria-containing smear layer into their bonding mechanism. The antibacterial effects of the self-etching primers Clearfil SE Bond, Mac Bond, Imperva FL Bond, One-Up BondF and Prompt L-Pop were evaluated using the bacteria Streptococcus mutans ATCC25175, Peptostreptococcus anaerobius, Peptostreptococcus prevotii, Peptostreptococcus asaccharolyticus, Lactobacillus acidophilus, Lactobacillus catenaforme, Lactobacillus jensenii, Actinomyces odontolyticus, Porphyromonas endodontalis, Clostridium ramosum, Prevotella oris, Prevotella denticola and Fusobacterium nucleatum, with a disk diffusion method. A single-bottle total-etch dentin adhesive (Excite) was used for comparisons and chlorhexidine (0.2%) was used as the positive control. After incubation, zones of inhibited bacterial growth were observed. One-Up BondF, Prompt L-Pop and Excite showed growth inhibition for all bacterial strains. The bonding agents of Clearfil SE Bond, Mac Bond and Imperva FL Bond were unable to inhibit the growth of Lactobacillus jensenii and Actinomyces odontolyticus, while the primers of these systems produced inhibition halos to all tested microorganisms greater than that of chlorhexidine.
Topics: Actinomyces; Alkanes; Anti-Infective Agents, Local; Chlorhexidine; Clostridium; Dentin-Bonding Agents; Fusobacterium nucleatum; Gram-Negative Bacteria; Gram-Positive Bacteria; Humans; Lactobacillus; Lactobacillus acidophilus; Maleates; Materials Testing; Methacrylates; Peptostreptococcus; Porphyromonas; Prevotella; Resin Cements; Statistics, Nonparametric; Streptococcus mutans
PubMed: 12670069
DOI: No ID Found -
Intensive Care Medicine May 2003
Topics: Bacteremia; Bacteroidaceae Infections; Humans; Intensive Care Units; Periodontal Abscess; Prevotella; Sepsis
PubMed: 12664220
DOI: 10.1007/s00134-003-1697-z -
Hua Xi Kou Qiang Yi Xue Za Zhi = Huaxi... Feb 2001The study was designed to evaluate the antiseptic efficacy of Tinidazole stilus against periodontal pathogen.
OBJECTIVE
The study was designed to evaluate the antiseptic efficacy of Tinidazole stilus against periodontal pathogen.
METHODS
A total of 41 patients with periodontitis were included in this study. 26 of them were treated by Tindazole stilus and other 15 cases were treated by metronidazole stilus as controls. The prevalence of subgingival bacteria from both group were examined.
RESULTS
The eliminate rates for Black pigmented anaerobic rod (Porphyromonas gingivalis, Prevotella intermedius, Prevotella melaninogenicus), Fusobacteriums, Prevotella oris, Prevotella oralis, Eubacterium and Actinomyces were 95.8% 94.1%, 83.3% and 76.5%. The anti-bacterial efficiency of Tinidazole against periodontal pathogen was significantly higher than that of metronidazole (P < 0.05).
CONCLUSION
It appears to be effective to use Tinidazole stilus as local application against periodontal pathogens.
Topics: Administration, Topical; Adult; Aged; Anti-Bacterial Agents; Female; Humans; Male; Metronidazole; Middle Aged; Periodontitis; Porphyromonas gingivalis; Prevotella melaninogenica; Tinidazole
PubMed: 12539636
DOI: No ID Found -
Journal of Endodontics Dec 2001Past concepts that the pulp does not become infected until an actual carious exposure takes place have been challenged. The antibacterial effects of the dentin bonding... (Comparative Study)
Comparative Study
Past concepts that the pulp does not become infected until an actual carious exposure takes place have been challenged. The antibacterial effects of the dentin bonding systems Single Bond, Prime&Bond NT, and Excite were evaluated using the bacteria Streptococcus mutans ATCC 25175, Streptococcus intermedius, Lactobacillus acidophilus, Prevotella oris, Prevotella bivia, Prevotella denticola, Porphyromonas gingivalis, Porphyromonas endodontalis, and Clostridium ramosum with a disk diffusion method. Chlorhexidine (0.2%) was used as a positive control. After incubation zones of inhibited bacterial growth were measured. Prime&Bond NT showed growth inhibition for all bacterial strains. Lactobacillus acidophilus and Streptococcus mutans were remarkably resistant to Single Bond, whereas EX produced no inhibitory effect on Porphyromonas endodontalis, although the adhesive produced the maximum halo inhibition to Streptococcus mutans (15+/-1 mm), showing an antibacterial effect closest to chlorhexidine. The variety of results obtained in this study suggest that antibacterial properties of current dentin adhesives may depend on components that are originally incorporated to promote adhesion.
Topics: Anti-Infective Agents, Local; Bacteria, Anaerobic; Bisphenol A-Glycidyl Methacrylate; Dentin-Bonding Agents; Methacrylates; Microbial Sensitivity Tests; Polymethacrylic Acids; Statistics, Nonparametric
PubMed: 11771577
DOI: 10.1097/00004770-200112000-00004 -
Mund-, Kiefer- Und Gesichtschirurgie :... May 2000Out of 440 dentogenic pyogenic infections, 171 exclusively caused by anaerobes were investigated to understand the importance of anaerobic bacteria in dental pyogenic...
Out of 440 dentogenic pyogenic infections, 171 exclusively caused by anaerobes were investigated to understand the importance of anaerobic bacteria in dental pyogenic processes better. Grampositive anaerobic bacteria dominated. The predominant grampositive isolates in monoinfections were Peptostreptococci and in the case of mixed infections, strains of the genus Eubacterium. Strains of Prevotella and Porphyromonas dominated the gramnegative anaerobic spectra. The resistance to penicillin was very low. Altogether, only one strain of Prevotella oris and one strain of Prevotella oralis showed resistance to penicillin.
Topics: Bacteria, Anaerobic; Bacterial Infections; Bacteriological Techniques; Humans; Microbial Sensitivity Tests; Penicillin Resistance; Periapical Abscess; Periodontal Abscess; Periodontitis; Retrospective Studies
PubMed: 10900958
DOI: 10.1007/s100060050188