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Antonie Van Leeuwenhoek Dec 2015In the context of studying the influence of N-fertilization on N2 and N2O flux rates in relation to the soil bacterial community composition in fen peat grassland, a...
In the context of studying the influence of N-fertilization on N2 and N2O flux rates in relation to the soil bacterial community composition in fen peat grassland, a group of bacterial strains was isolated that performed dissimilatory nitrate reduction to ammonium and concomitantly produced N2O. The amount of nitrous oxide produced was influenced by the C/N ratio of the medium. The potential to generate nitrous oxide was increased by higher availability of nitrate-N. Phylogenetic analysis based on the 16S rRNA and the rpoB gene sequences demonstrated that the investigated isolates belong to the genus Proteus, showing high similarity with the respective type strains of Proteus vulgaris and Proteus penneri. DNA-DNA hybridization studies revealed differences at the species level. These differences were substantiated by MALDI-TOF MS analysis and several distinct physiological characteristics. On the basis of these results, it was concluded that the soil isolates represent a novel species for which the name Proteus terrae sp. nov. (type strain N5/687(T) =DSM 29910(T) =LMG 28659(T)) is proposed.
Topics: Ammonia; Bacterial Typing Techniques; Carbon; Cluster Analysis; Culture Media; DNA, Bacterial; DNA, Ribosomal; DNA-Directed RNA Polymerases; Deinococcus; Molecular Sequence Data; Nitrates; Nitrogen; Nitrous Oxide; Nucleic Acid Hybridization; Phylogeny; Proteus; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Soil Microbiology; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
PubMed: 26437638
DOI: 10.1007/s10482-015-0601-5 -
Carbohydrate Research Apr 2015Proteus penneri is an opportunistic pathogen, which may cause severe diseases, most frequently urinary tract infections in immunocompromised patients. P. penneri Br 114...
Proteus penneri is an opportunistic pathogen, which may cause severe diseases, most frequently urinary tract infections in immunocompromised patients. P. penneri Br 114 exhibiting a good swarming growth ability as an S-form strain was isolated from a wound of a patient in Łódź, Poland. Serological studies using ELISA and Western blotting and chemical analyses along with (1)H and (13)C NMR spectroscopy showed that the O-antigen (O-polysaccharide) of this strain is unique among the known Proteus serotypes O1-O79. It possesses a linear pentasaccharide repeating unit containing a partially O-acetylated amide of D-glucuronic acid (GlcA) with L-serine having the following structure: [structure: see text]. These data are a basis for creating a new Proteus serogroup, O80, so far represented by the single Br 114 isolate. The O80 is the 21st O-serogroup containing P. penneri strains and the fourth serogroup based on Proteus spp. clinical isolates from Łódź, Poland.
Topics: Carbohydrate Sequence; Humans; Magnetic Resonance Spectroscopy; Male; Middle Aged; O Antigens; Poland; Proteus Infections; Proteus penneri; Serotyping
PubMed: 25771295
DOI: 10.1016/j.carres.2015.02.003 -
Indian Journal of Pathology &... 2014Indole negative Proteus species are invariably incorrectly identified as Proteus mirabilis, often missing out isolates of Proteus penneri. We report a case of extended...
Indole negative Proteus species are invariably incorrectly identified as Proteus mirabilis, often missing out isolates of Proteus penneri. We report a case of extended spectrum beta lactamase producing and multidrug-resistant P. penneri isolated from pus from pressure sore of a patient of road traffic accident. Correct and rapid isolation and identification of such resistant pathogen are important as they are significant nosocomial threat.
Topics: Accidents, Traffic; Adult; Bacteriological Techniques; Diagnostic Errors; Drug Resistance, Multiple, Bacterial; Humans; Male; Proteus Infections; Proteus penneri; Wound Infection; Wounds and Injuries; beta-Lactamases
PubMed: 25118755
DOI: 10.4103/0377-4929.138791 -
Acta Biochimica Polonica 2014Most Proteus lipopolysaccharides (LPSs) contain uronic acids or their amides with different amino acids, which together with other negatively charged components account...
Most Proteus lipopolysaccharides (LPSs) contain uronic acids or their amides with different amino acids, which together with other negatively charged components account for the acidic character of such LPS molecules. Previous studies have shown the significance of an amide of galacturonic acid with lysine [D-GalA(L-Lys)] for serological specificity of O-antigens from few P. mirabilis strains. In this work, the immunodominant role of GalALys was indicated for the P. penneri 42 LPS core region. The studies also showed the serological identity of core oligosaccharides from P. penneri 42 (O71), P. mirabilis 51/57 (O28) and R14/S1959 strains.
Topics: Amides; Hexuronic Acids; Immunodominant Epitopes; Lipopolysaccharides; Lysine; O Antigens; Proteus penneri
PubMed: 24644555
DOI: No ID Found -
Genome Announcements Jan 2014Proteus hauseri ZMd44 (CGMCC 6746), as a crucial biodecolorizing, bioelectricity-generating, and copper-resistant bacterium, is distinguished from the urinary pathogens...
Proteus hauseri ZMd44 (CGMCC 6746), as a crucial biodecolorizing, bioelectricity-generating, and copper-resistant bacterium, is distinguished from the urinary pathogens Proteus penneri and Proteus mirabilis. To further investigate the genetic functions of this strain, the genome sequence and annotation of its open reading frames, which consist of 3,875,927 bp (G+C content, 38.12%), are presented here.
PubMed: 24435854
DOI: 10.1128/genomeA.00992-13 -
Antonie Van Leeuwenhoek Feb 2014Bacteriosis has become a major economic problem in the farming of the Pacific white shrimp Penaeus vannamei. However, no definitive data are available about Proteus...
Identification of a Proteus penneri isolate as the causal agent of red body disease of the cultured white shrimp Penaeus vannamei and its control with Bdellovibrio bacteriovorus.
Bacteriosis has become a major economic problem in the farming of the Pacific white shrimp Penaeus vannamei. However, no definitive data are available about Proteus penneri infection in cultured P. vannamei and its control. In this study, a virulent strain NC was isolated from diseased P. vannamei suffering from red body disease and identified as a P. penneri isolate through phylogenetic analysis and ATB 32GN system. A phylogenetic constructed tree using the neighbour-joining method identified the NC isolate as a P. penneri strain. In addition, Bdellovibrio bacteriovorus conferred significant protection against P. penneri: it exhibited significant bacteriolytic effects on the pathogenic P. penneri, had a wide prey range towards Proteus pathogens, and displayed a good protective efficacy on experimental P. penneri infection in P. vannamei. To the best of our knowledge, this is the first report of farmed P. vannamei infected with P. penneri and its control with B. bacteriovorus.
Topics: Animals; Antibiosis; Bacterial Typing Techniques; Bacteriolysis; Bdellovibrio; Cluster Analysis; DNA, Bacterial; DNA, Ribosomal; Molecular Sequence Data; Penaeidae; Pest Control, Biological; Phylogeny; Proteus penneri; RNA, Ribosomal, 16S; Sequence Analysis, DNA
PubMed: 24271474
DOI: 10.1007/s10482-013-0079-y -
Spectrochimica Acta. Part A, Molecular... Jan 2014A novel ligand (E)-2-styryl-1H-imidazo [4, 5-f] [1, 10] phenanthroline(L) has been synthesized from 1,10-phenanthroline-5,6-dione. Its transition metal complexes,...
A novel ligand (E)-2-styryl-1H-imidazo [4, 5-f] [1, 10] phenanthroline(L) has been synthesized from 1,10-phenanthroline-5,6-dione. Its transition metal complexes, [FeLCl4][L-H] and [CuL2](NO3)2 have also been synthesized. Besides, three mixed ligand lanthanide metal complexes of Phendione and β-diketones have been synthesized, namely [Eu(TFN)3(Phendione)] (TFN = 4,4,4-trifluoro-1(2-napthyl)-1,3-butanedione), [Eu(HFT)3(Phendione)] (HFT = 4,4,5,5,6,6,6-heptafluoro-1-(2-thienyl)-1,3-hexanedione), [Yb(HFA)3(Phendione)] (hfa = hexafluoroacetylacetonate). The synthesized ligands and metal complexes have been characterized by FTIR, UV-Visible spectroscopy and PL spectra. DNA binding activities of the complexes and the ligands have been studied by DNA gel electrophoresis. DNA binding studies showed that Fe complex of the synthesized ligand is more potent DNA binding and damaging agent compare to others under study. The synthesized compounds were also screened for their antimicrobial activities by disc diffusion method against three microbes, namely Escherichia coli, Staphylococcus aureus, Proteus penneri. The lanthanide complexes of phendione showed great antibacterial activities.
Topics: Anti-Infective Agents; Bacterial Infections; Coordination Complexes; DNA; Escherichia coli; Humans; Luminescence; Microbial Sensitivity Tests; Phenanthrolines; Proteus; Staphylococcus aureus; Transition Elements
PubMed: 24184579
DOI: 10.1016/j.saa.2013.09.110 -
Serological studies of Proteus penneri strains determining qualification to appropriate O-serogroup.Polish Journal of Microbiology 2013Our Department of General Microbiology created a wide collection of P. penneri isolates and classified most of them into 19 different O-serogroups. This work describes...
Our Department of General Microbiology created a wide collection of P. penneri isolates and classified most of them into 19 different O-serogroups. This work describes the classification of 12 remaining P. penneri strains. The lipopolysaccharides extracted from P. penneri strains were tested in an enzyme-linked immunosorbent assay (ELISA) with selected O-antisera against Proteus sp. strains. Homologous and cross-reacting systems were checked in: passive immunohemolysis (PIH), inhibition of ELISA and PIH and Western blot procedure. These studies led to the qualification of tested P. penneri strains to five Proteus sp. O-serogroups, thus completing the serological classification of the whole collection.
Topics: Enzyme-Linked Immunosorbent Assay; Humans; Lipopolysaccharides; Proteus penneri; Serotyping
PubMed: 24053026
DOI: No ID Found -
Carbohydrate Research Oct 2013Proteus penneri, like the other seven species from the genus, are Gram-negative, peritrichously flagellated rods capable of swarming growth on humid solid media. These... (Review)
Review
Proteus penneri, like the other seven species from the genus, are Gram-negative, peritrichously flagellated rods capable of swarming growth on humid solid media. These bacteria are human opportunistic pathogens involved in many infections but they mainly affect the urinary tract of hospitalized, long-term catheterized patients. P. penneri rods produce a lot of virulence factors, among which the lipopolysaccharide seems to be the most interesting due to its structural and serological diversity. From the three LPS regions of P. penneri strains only the core region and O-specific polysaccharide (OPS) were structurally and serologically examined. P. penneri LPS core region is characterized by a common inner part representing the III glycoform and a diverse distal part (12 different structures). The P. penneri O-antigens contain sugar and non-sugar compounds and some of them rarely occur in nature. In both P. penneri LPS regions putative epitopes have been pointed out. Serospecificity of OPS allowed classifying many P. penneri isolates to different Proteus sp. O-serogroups, among which 12 contain P. penneri strains only.
Topics: Animals; Carbohydrate Sequence; Environment; Humans; Immunochemistry; Lipopolysaccharides; Molecular Sequence Data; Proteus; Vaccination; Virulence Factors
PubMed: 23896159
DOI: 10.1016/j.carres.2013.06.025 -
Advances in Clinical and Experimental... 2013Proteus sp. strains isolated from patients with urinary tract infection (UTI) are often insensitive to the bactericidal action of normal human serum (NHS) which poses a...
BACKGROUND
Proteus sp. strains isolated from patients with urinary tract infection (UTI) are often insensitive to the bactericidal action of normal human serum (NHS) which poses a clinical problem. The swarming phenomenon is an especially important factor in cases of UTIs gained through the ascending route. Both these virulence factors are connected with the cell surface components of bacteria, including lipopolysaccharide (LPS).
OBJECTIVES
The resistance of Proteus bacilli to the bactericidal activity of NHS and the swarming phenomenon were investigated as well as the possible relationships between these virulence factors and the chemical structure of LPS.
MATERIAL AND METHODS
The research was carried out on P. penneri and P. vulgaris species. Two preparations of sera were tested with respect to the bactericidal action of NHS. The ability of bacteria to swarm was checked on broth agar plates. The length of the O-specific part of LPS was estimated after poliacrylamide gel electrophoresis (PAGE) and staining with silver nitrate.
RESULTS
Among the 62 tested Proteus strains, over 62% of Proteus vulgaris and 50% of Proteus penneri strains were sensitive to the bactericidal action of NHS. However, the number of resistant strains grew dramatically when serum with blocked complement activation via the alternative pathway was used. From 102 of the Proteus sp. Strains, only few were unable to swarm over the solid surface of the media. The remaining showed diverse ability to translocate.
CONCLUSIONS
There was no definite correlation between the chemical structure of the O-specific chains of lipopolysaccharides and sensitivity or resistance of the Proteus sp. strains to NHS. Also, no significant relationships were found between the length or the chemical structure of the O-specific chains of the bacterial LPSs and the swarming phenomenon.
Topics: Blood Bactericidal Activity; Humans; Lipopolysaccharides; Locomotion; Proteus Infections; Proteus penneri; Proteus vulgaris; Serum Bactericidal Test; Urinary Tract Infections; Virulence Factors
PubMed: 23709372
DOI: No ID Found