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The Pan African Medical Journal 2013Roseomonas is a pink-pigmented, nonfermentative, oxidative, Gram-negative coccobacilli that has clinical importance as opportunistic pathogen which can lead to...
Roseomonas is a pink-pigmented, nonfermentative, oxidative, Gram-negative coccobacilli that has clinical importance as opportunistic pathogen which can lead to infections especially in immunosuppressed individuals. It is relatively less reported in many centers. These microorganisms are detected after several days growth in culture environment, and typical pink, mucoid colonies are detected. We are reported a case of cranioplasty infection that took place in a patient with with cranial abscess formation due to Roseomonas gilardii at Izmir University School of Medicine Medicalpark Hospital.
Topics: Abscess; Adult; Bone Diseases; Gram-Negative Bacterial Infections; Hospitals, University; Humans; Male; Methylobacteriaceae; Postoperative Complications; Skull; Turkey
PubMed: 23819004
DOI: 10.11604/pamj.2013.14.162.2730 -
International Journal of Systematic and... Jun 2013An aerobic, Gram-stain-negative, motile, mesophilic bacterium, designated strain 7515T-07(T), was isolated from an air sample in the Taean region, Republic of Korea. The...
An aerobic, Gram-stain-negative, motile, mesophilic bacterium, designated strain 7515T-07(T), was isolated from an air sample in the Taean region, Republic of Korea. The strain grew at 4-40 °C (optimum, 30 °C) and pH 5.0-9.0 (optimum, pH 7.0). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 7515T-07(T) was related to members of the genus Roseomonas and had highest 16S rRNA gene sequence similarity with Roseomonas ludipueritiae 170/96(T) (96.7%). 16S RNA gene sequence similarity between strain 7515T-07(T) and Roseomonas gilardii ATCC 49956(T) (the type species of the genus Roseomonas) was 93.4%. Strain 7515T-07(T) contained Q-10 as the ubiquinone and C18:1ω7c and C19:0 cyclo ω8c as the dominant fatty acids (>10%). The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and two unidentified aminolipids. The DNA G+C content was 73.0 mol%. Combined data from phenotypic, phylogenetic and chemotaxonomic studies demonstrated that strain 7515T-07(T) is a representative of a novel species of the genus Roseomonas, for which the name Roseomonas aerophila sp. nov. is proposed. The type strain is 7515T-07(T) (=KACC 16529(T)=NBRC 108923(T)).
Topics: Air Microbiology; Bacterial Typing Techniques; Base Composition; DNA, Bacterial; Fatty Acids; Methylobacteriaceae; Molecular Sequence Data; Phylogeny; RNA, Ribosomal, 16S; Republic of Korea; Sequence Analysis, DNA; Ubiquinone
PubMed: 23178724
DOI: 10.1099/ijs.0.046482-0 -
Journal of Medical Microbiology Nov 2009The genus Roseomonas comprises groups of slow-growing, Gram-negative coccobacilli, which only infrequently cause infection in humans. When identified, they are...
The genus Roseomonas comprises groups of slow-growing, Gram-negative coccobacilli, which only infrequently cause infection in humans. When identified, they are associated with immunocompromised adults, often causing bacteraemia. Due to their rarity, members of this genus can be overlooked or misidentified using automated laboratory identification systems. We report on an immunocompetent adolescent patient who developed septic arthritis due to Roseomonas gilardii following surgery for a sports injury. The isolate was initially misidentified as Bordetella bronchiseptica using the Vitek 2 system, but confirmed as R. gilardii based on 16S rRNA gene sequencing. To the best of our knowledge, this is the first case of a healthy paediatric patient with septic arthritis due to R. gilardii.
Topics: Acetobacteraceae; Adolescent; Arthritis, Infectious; Female; Gram-Negative Bacterial Infections; Humans; Immunocompetence; Knee Joint; RNA, Ribosomal, 16S; Sequence Analysis, DNA
PubMed: 19574413
DOI: 10.1099/jmm.0.011106-0 -
International Journal of Systematic and... Jul 2009A non-motile, coccobacilli-shaped, pale-pink-pigmented bacterium, designated strain CW67(T), was isolated from a water-cooling system in Gwangyang, Republic of Korea....
A non-motile, coccobacilli-shaped, pale-pink-pigmented bacterium, designated strain CW67(T), was isolated from a water-cooling system in Gwangyang, Republic of Korea. Cells were found to be Gram-negative, catalase-positive and oxidase-positive, the major fatty acids were C(18 : 1)omega7c (43.6 %) and C(16 : 0) (15.8 %), the predominant respiratory lipoquinone was Q-10 and the DNA G+C content was 69.5 mol%. A phylogenetic tree based on 16S rRNA gene sequence comparisons showed that strain CW67(T) forms an evolutionary lineage within the radiation of the genus Roseomonas and that its closest relative is Roseomonas gilardii subsp. rosea MDA5605(T) (94.7 % sequence similarity). Evidence from this polyphasic study showed that strain CW67(T) could not be assigned to any recognized species. It therefore represents a novel species, for which the name Roseomonas frigidaquae sp. nov. is proposed, with CW67(T) (=KCTC 22211(T) =JCM 15073(T)) as the type strain.
Topics: Acetobacteraceae; Bacterial Typing Techniques; Base Composition; DNA, Bacterial; Fatty Acids; Fresh Water; Genes, rRNA; Korea; Molecular Sequence Data; Phenotype; Phylogeny; RNA, Ribosomal, 16S; Refrigeration; Sequence Analysis, DNA; Species Specificity
PubMed: 19542147
DOI: 10.1099/ijs.0.004812-0 -
International Journal of Systematic and... May 2009Phylogenetic analyses based on 16S rRNA gene sequences revealed that Teichococcus ludipueritiae and Muricoccus roseus are closely related to the species of the genus...
Transfer of Teichococcus ludipueritiae and Muricoccus roseus to the genus Roseomonas, as Roseomonas ludipueritiae comb. nov. and Roseomonas rosea comb. nov., respectively, and emended description of the genus Roseomonas.
Phylogenetic analyses based on 16S rRNA gene sequences revealed that Teichococcus ludipueritiae and Muricoccus roseus are closely related to the species of the genus Roseomonas. The type strain of Teichococcus ludipueritiae, 170/96(T), exhibited 16S rRNA gene sequence similarity levels of 96.4 % to Roseomonas cervicalis ATCC 49957(T), 95.0 % to Roseomonas aquatica TR53(T), 94.5 % to Muricoccus roseus 173/96(T), 93.4 % to Roseomonas mucosa ATCC BAA-692(T) and 93.5 % to Roseomonas gilardii subsp. gilardii ATCC 49956(T), while Muricoccus roseus 173/96(T) showed 16S rRNA gene sequence similarity values of 95.7 % to R. mucosa ATCC BAA-692(T), 95.7 % to R. aquatica TR53(T) and 95.3 % to R. gilardii subsp. gilardii ATCC 49956(T) and R. gilardii subsp. rosea ATCC BAA-691(T). Different phylogenetic analysis methods (neighbour-joining, maximum-likelihood and maximum-parsimony) confirmed that both species are within the Roseomonas branch. Neither polyamine patterns (spermidine predominant) nor major characteristics in the polar lipid profiles distinguished the two species from representatives of the genus Roseomonas. The fatty acid composition of the two species exhibited alphaproteobacterial characteristics but, like Roseomonas species, they also showed considerable amounts of the rarely encountered C(18 : 1) 2-OH. On the other hand, they showed some phenotypic differences, but their features are compatible with the transfer of these two species to the genus Roseomonas. We propose the reclassification of Teichococcus ludipueritiae and Muricoccus roseus as Roseomonas ludipueritiae comb. nov. (type strain 170/96(T) =CIP 107418(T) =DSM 14915(T)) and Roseomonas rosea comb. nov. (type strain 173/96(T) =CIP 107419(T) =DSM 14916(T)), respectively. Emended descriptions of the genus Roseomonas and the species Roseomonas gilardii (and its subspecies Roseomonas gilardii subsp. gilardii and Roseomonas gilardii subsp. rosea), Roseomonas aquatica, Roseomonas cervicalis, Roseomonas mucosa and Roseomonas lacus are provided.
Topics: Bacterial Typing Techniques; DNA, Bacterial; Fatty Acids; Genes, rRNA; Methylobacteriaceae; Phenotype; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Species Specificity
PubMed: 19406818
DOI: 10.1099/ijs.0.004820-0 -
International Journal of Systematic and... Jun 2008A polyphasic study was carried out to clarify the taxonomic position of a pink-coloured, Gram-negative bacterium isolated from air in the Suwon region of Korea. The...
A polyphasic study was carried out to clarify the taxonomic position of a pink-coloured, Gram-negative bacterium isolated from air in the Suwon region of Korea. The novel strain, 5420S-30(T), grew in the temperature range of 5-35 degrees C (optimum 30 degrees C) and pH range of 5-9 (optimum pH 6-7). Growth did not occur in the presence of as little as 1 % (w/v) NaCl. It contained Q-10 as the predominant ubiquinone. The major fatty acids were C(18 : 1)omega7c, summed feature 3 (C(16 : 1)omega7c/iso-C(15 : 0) 2-OH) and C(16 : 0) (together representing 77.7 % of the total). The DNA G+C content was 72.1 mol%. The 16S rRNA gene sequence similarities between strain 5420S-30(T) and members of the genus Roseomonas were in the range 91.6-95.1 %, with the highest sequence similarities (95.1 and 94.5 %, respectively) to Roseomonas aquatica TR53(T) and Roseomonas gilardii ATCC 49956(T). On the basis of its phenotypic and genotypic characteristics, strain 5420S-30(T) represents a novel species of the genus Roseomonas, for which the name Roseomonas aerilata sp. nov. is proposed, with 5420S-30(T) (=KACC 12521(T) =DSM 19363(T)) as the type strain.
Topics: Air Microbiology; Bacterial Typing Techniques; DNA, Bacterial; DNA, Ribosomal; Fatty Acids; Genes, rRNA; Genotype; Korea; Methylobacteriaceae; Molecular Sequence Data; Phenotype; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Species Specificity
PubMed: 18523199
DOI: 10.1099/ijs.0.65385-0 -
The Southeast Asian Journal of Tropical... Sep 2007Roseomonas is a pink-pigmented, non-fermentative, gram-negative coccobacillus bacterium. Human infections caused by Roseomonas are very rare. We report the first case of...
Roseomonas is a pink-pigmented, non-fermentative, gram-negative coccobacillus bacterium. Human infections caused by Roseomonas are very rare. We report the first case of bacteremia associated with Roseomonas gilardii subsp rosea in Thailand. The bacterium was isolated from blood culture and identified by cellular morphology, characteristics of colonies on blood agar, extensive biochemical tests and 16S ribosomal DNA sequencing.
Topics: Aged, 80 and over; Bacteremia; Gram-Negative Bacterial Infections; Humans; Male; Methylobacteriaceae; RNA, Ribosomal, 16S; Thailand
PubMed: 18041307
DOI: No ID Found -
Microbiology and Immunology 2007Strain K-20, a Gram-negative, non-motile, non-spore-forming and strictly aerobic rod, which produces a pale pink pigment, was isolated from biofilm in a cooling tower in...
Strain K-20, a Gram-negative, non-motile, non-spore-forming and strictly aerobic rod, which produces a pale pink pigment, was isolated from biofilm in a cooling tower in Tokyo, Japan. The taxonomic feature of the strain was studied using phenotypic tests and phylogenetic analysis. Phylogenetic analysis of 16S rRNA gene sequences showed that the strain was related to Roseomonas gilardii subsp. rosea, Roseomonas gilardii subsp. gilardii, Roseomonas cervicalis and Roseomonas mucosa at 94.3-94.6 sequence similarities. Growth occurred at 25-40 C and pH 5.0-10.0, optimal at 35 C and pH 7.0. Growth did not occur in the presence of >or=2% NaCl. The API 20NE identification system gave a positive result for urease, L-arabinose, potassium gluconate, adipic acid, malic acid and trisodium citrate (API code number 0201465). The predominant fatty acids of strain K-20 were C18:1Delta11 (50.8%) and C16:1 (17.2%). Cells contained ubiquinone 10 (Q-10) as the major quinone and the G+C content was 72.0 mol%. Based on phenotypic, chemotaxonomic and phylogenetic data, it was assumed that strain K-20 (=JCM 14634) is a novel species of the genus Roseomonas.
Topics: Base Composition; Base Sequence; Biofilms; DNA, Bacterial; Fatty Acids; Methylobacteriaceae; Microbial Sensitivity Tests; Molecular Sequence Data; Phylogeny; Pigmentation; Polymerase Chain Reaction; RNA, Ribosomal, 16S; Sequence Alignment; Tokyo; Water Microbiology
PubMed: 17579276
DOI: 10.1111/j.1348-0421.2007.tb03951.x -
International Journal of Systematic and... Oct 2006Strain TR53(T), a Gram-negative, non-motile, non-spore-forming and strictly aerobic coccobacillus, isolated from the drinking water distribution system of Seville,...
Strain TR53(T), a Gram-negative, non-motile, non-spore-forming and strictly aerobic coccobacillus, isolated from the drinking water distribution system of Seville, Spain, was subjected to polyphasic taxonomic analysis using a combination of phenotypic, genotypic and phylogenetic features. Phylogenetic analysis of 16S rRNA gene sequences showed that strain TR53(T) had highest similarity to members of the genus Roseomonas, with sequence similarity values between 95.7 % (to Roseomonas genomospecies 5 strain ATCC 49960) and 94.0 % (to Roseomonas gilardii subsp. rosea ATCC 49956(T)). On the basis of its phenotypic characteristics, 16S rRNA gene sequence data and DNA G+C content (68.6 mol%), strain TR53(T) represents a novel species of the genus Roseomonas, for which the name Roseomonas aquatica sp. nov. is proposed. The type strain of Roseomonas aquatica is TR53(T) (=CECT 7131(T)=JCM 13556(T)).
Topics: Bacterial Typing Techniques; Base Composition; DNA, Bacterial; DNA, Ribosomal; Fresh Water; Genes, rRNA; Methylobacteriaceae; Molecular Sequence Data; Phenotype; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Spain; Water Supply
PubMed: 17012549
DOI: 10.1099/ijs.0.64379-0 -
International Journal of Systematic and... Jan 2006Strain CP2CT was isolated from biological soil crusts in the Colorado Plateau, USA. The isolate was aerobic, facultatively fermentative, Gram-negative, non-motile and...
Strain CP2CT was isolated from biological soil crusts in the Colorado Plateau, USA. The isolate was aerobic, facultatively fermentative, Gram-negative, non-motile and red-pigmented (due to the presence of carotenoids), but did not contain bacteriochlorophyll a. The strain tested positive for catalase, oxidase and urease and was negative for lysine and ornithine decarboxylases and arginine dihydrolase. The major fatty acids present were C(18 : 1)omega7c and C(16 : 0). It had a high DNA G+C content of 75 mol%. Comparisons of 16S rRNA gene sequences identified bacteriochlorophyll a-producing strains of Paracraurococcus ruber (94.9 %), Craurococcus roseus (92.2 %) and Roseococcus thiosulfatophilus (92.3 %), as well as non-bacteriochlorophyll a-producing bacteria Muricoccus roseus (94.9 %), Roseomonas gilardii (94.2 %) and Roseomonas mucosa (93.8 %), as the bacteria most closely related to strain CP2CT. Phylogenetically, CP2CT was placed roughly equidistantly from the above organisms. Based on its phylogenetic placement and morphological and physiological characteristics, strain CP2CT is assigned to a new genus in the alpha-1 subgroup of the Proteobacteria, for which the name Belnapia gen. nov. is proposed. Strain CP2CT (= ATCC BAA-1043T = DSM 16746T) is proposed as the type strain of the type species of this genus, with the name Belnapia moabensis gen. nov., sp. nov.
Topics: Alphaproteobacteria; Bacterial Proteins; Base Composition; Catalase; Colorado; Fatty Acids; Fermentation; Molecular Sequence Data; Oxidoreductases; RNA, Bacterial; RNA, Ribosomal, 16S; Sequence Homology, Nucleic Acid; Soil Microbiology; Species Specificity; Urease
PubMed: 16403866
DOI: 10.1099/ijs.0.63764-0