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The Journal of Parasitology Dec 1979Merozoites were found in blood smears from calves, lambs, and pigs exhibiting signs of acute sarcocystosis after oral infection with sporocysts of Sarcocystis bovicanis,...
Merozoites were found in blood smears from calves, lambs, and pigs exhibiting signs of acute sarcocystosis after oral infection with sporocysts of Sarcocystis bovicanis, Sarcocystis ovicanis, and Sarcocystis suihominis. In each of 3 experiments, whole blood containing merozoites was transfused from an infected host to 2 uninfected recipients of the same species; 2 additional animals of the same species served as uninfected nontransfused controls. The bovine, ovine, and porcine donors all died of acute sarcocystosis. Clinical signs of sarcocystosis were not seen in any transfusion recipients or controls. All recipients and controls were killed and histologic specimens of esophagus, diaphragm, heart, skeletal muscle, and tongue were examined microscopically. Large numbers of intramuscular cysts were present in transfusion recipients, whereas few or no cysts were present in controls, indicating that S. bovicanis, S. ovicanis, and S. suihominis had been transmitted between intermediate hosts of the same species by blood transfusion.
Topics: Animals; Blood Transfusion; Cattle; Cattle Diseases; Sarcocystis; Sarcocystosis; Sheep; Sheep Diseases; Swine; Swine Diseases; Transfusion Reaction
PubMed: 120435
DOI: No ID Found -
Immunitat Und Infektion Nov 1979Eleven medical students and six members of the Institute of Medical Parasitology, University of Bonn participated in a meal with raw pork of an experimentally...
Eleven medical students and six members of the Institute of Medical Parasitology, University of Bonn participated in a meal with raw pork of an experimentally Sarcocystis suihominis infected pig. During the first two days the test persons suffered from the same symptoms as a previous group (see Piekarski et al., 1978). The severity of the symptoms was related ito certain degree to the quantity of the pork comsumed, but the individual reaction varied considerably; one test person with a higher amount of meat remained healthy. Apparently, only excessively high quantities of infected meat lead to severe symptoms. Lower dosages cause a protracted course of the disease. In general, the Sarcosporidia infection produces a transitory disease which quickly disapprears without remaining after-effects.
Topics: Alanine Transaminase; Animals; Aspartate Aminotransferases; Cattle; Diarrhea; Feces; Female; Humans; L-Lactate Dehydrogenase; Male; Sarcocystis; Sarcocystosis; Swine; Vomiting; Zoonoses
PubMed: 120308
DOI: No ID Found -
Zeitschrift Fur Parasitenkunde (Berlin,... Jun 1979Sporocysts of Sarcocystis suihominis obtained from human feces were used to infect swine. Heart, tongue, and skeletal muscle from experimentally infected and noninfected...
Sporocysts of Sarcocystis suihominis obtained from human feces were used to infect swine. Heart, tongue, and skeletal muscle from experimentally infected and noninfected control swine were fed via stomach tube to nonhuman primates including chimpanzees (Pan troglodytes), rhesus monkeys (Macaca mulatta), and cynomolgus monkeys (Macaca irus). All primates fed infected swine tissues shed sporocysts beginning 13 to 15 days postinfection and were still shedding sporocysts at the conclusion of the experiment, 30 days postinfection. Rhesus and cynomolgus monkeys were fed infected swine tissues a second time and shed sporocysts. All primates remained in good health throughout both experiments and exhibited no unusual clinical signs as a result of infection.
Topics: Animals; Feces; Haplorhini; Humans; Macaca; Macaca mulatta; Monkey Diseases; Pan troglodytes; Sarcocystis; Sarcocystosis; Swine; Swine Diseases
PubMed: 113949
DOI: 10.1007/BF00927841 -
Zeitschrift Fur Parasitenkunde (Berlin,... Feb 1979Sexual stages and oocysts of Sarcocystis suihominis were developed in human tissue cultures and studied with the electron microscope. This development was extremely...
Sexual stages and oocysts of Sarcocystis suihominis were developed in human tissue cultures and studied with the electron microscope. This development was extremely rapid, being completed about 18--22 h post infection and there were no preceding schizogonic processes, thus confirming the earlier observations that schizogony is obligatorily restricted to the intermediate host in the sarcosporidian life cycle. Micro- and macrogamonts could be distinguished about 12 h post infection and were situated in a parasitophorous vacuole bounded by two membranes. These gamonts reached diameters of up to 10 micrometer. The large nucleus of every microgamont gave rise simultaneously to about 20--30 microgametes. Only dense projections of the nucleus were used as nuclei of microgametes. The microgametes were slender, about 4--5 micrometer long, and several were found to have three flagella, one of which was attached to the body for some distance. Besides these flagella additional microtubules were found and in several cases the attached flagellum was not complete and contained various numbers of single or paired microtubules. The macrogametes were bounded by two membranes and contained two types of inclusions similar to the wall-forming bodies known from the genus Eimeria. The oocysts were bounded by a wall consisting of a dense outer layer and four membranes, under which two other membranes covered the cytoplasm. Beginning from the 22nd h post infection a development similar to sporulation was noted inside these oocysts. This sporulation, i.e., the formation of two sporocysts inside an oocyst, was, however, not completed, probably due to the rapid degeneration of the parasitized host cell. The oocyst itself even appeared intact five days later.
Topics: Cell Nucleus; Culture Techniques; Fibroblasts; Flagella; Germ Cells; Humans; Intestines; Microscopy, Electron; Sarcocystis; Skin; Time Factors
PubMed: 107669
DOI: 10.1007/BF01951336 -
Zeitschrift Fur Parasitenkunde (Berlin,... Feb 1979Examination of the original descriptions of the species of Sarcocystis in cattle, sheep, and swine, and of isosporid oocysts shed sporulated by dogs, cats, man, and...
Examination of the original descriptions of the species of Sarcocystis in cattle, sheep, and swine, and of isosporid oocysts shed sporulated by dogs, cats, man, and other carnivores, has shown that it is not possible in most instances to identify unambiguously recently recognized taxa. The original descriptions are insufficient, and because no type specimens exist, could apply to two or more of the presently recognized taxa. We consider the following nomina dubia: Sarcocystis hirsuta S. miescheriana S. tenella S. cruzi S. bertrami Isospora bigemina (S. bigemina) I. hominis (S. hominis) I. buteonis (Frenkelia buteonis) Because the former type species, Sarcocystis miescheriana, is an indeterminate nomen dubium, we are proposing S. muris as the new type species. Historically, it was the first species described clearly and unambiguously even in the light of present knowledge, and the stages of its life cycle are probably completely known; it was the second species to be named. Old and recent descriptions are reviewed, and definitions are proposed for the following taxa: S. bovifelis S. bovicanis S. bovihominis S. ovifelis S. ovicanis S. muris (type species) S. suihominis S. suicanis S. equicanis Frenkelia microti F. glareoli for which neotypes will be prepared and deposited with designated institutions and curators. A new subfamily, Cystoisosporinae, is created.
Topics: Animals; Apicomplexa; Arvicolinae; Birds; Carnivora; Cats; Cattle; Dogs; Feces; Haplorhini; Horses; Humans; Intestines; Sarcocystis; Sheep; Swine; Terminology as Topic
PubMed: 107666
DOI: 10.1007/BF01951337 -
Zentralblatt Fur Bakteriologie,... Sep 1978Six young pigs were experimentally infected with 100000 sporocysts of Sarcocystis suicanis and killed on the 32nd, 47th, 55th, 64th, 81th and 90th day p.i. The...
Six young pigs were experimentally infected with 100000 sporocysts of Sarcocystis suicanis and killed on the 32nd, 47th, 55th, 64th, 81th and 90th day p.i. The ultrastructure of sarcocysts in the muscle cells was studied. Light microscopical preliminary examination on the 32nd day p.i. showed cysts which lay in muscle fibres and were surrounded by a fine membran. On the 55th day p.i. the cyst wall showed radial light and dark stripes of 2.6 micrometer thickness, which reached a thickness of 3.2 micrometer on the 90th day p.i. The electron microscopic study of the cyst wall on the 32nd day p.i. showed a unit membran the numerous small outward projections of which are underlined by osmiophilic material that is absent in the inwardly directed invaginations. During the growth of the cyst, this primary cyst wall became regularly folded, forming many protrusions on the 55th day p.i. These appeared palisade-like and contained 57--62 filamentous elements in a single layer under the primary wall at a distance of 10 nm. In the centre of the protrusions there were 5--8 groups, comprising 2--5 filaments each. They have their origin in the ground-substance. As these filaments maintain their arrangement also in old cysts, they can be regarded as characteristic of Sarcocystis suicanis. The groundsubstance is homogenous; on the 32nd day p.i. it contained only metrocytes, and developed septa towards the interior of the cyst on the 47th day p.i. The parasites were reproduced by endodyogeny and appeared very similar to the structure of all cyst forming coccidians. Some old cysts degenerated fattily and the host cells were destroyed. The cysts of Sarcocystis suicanis with complete development of the protrusions of the primary wall can thus be differentiated from cysts of Sarcocystis suihominis with certainty.
Topics: Animals; Dogs; Muscles; Sarcocystis; Sarcocystosis; Species Specificity; Swine
PubMed: 103340
DOI: No ID Found -
Immunitat Und Infektion Aug 1978Eight medical students ate for a test raw meat from a pig experimentally infected with sporocysts of Sarcocystis suihominis. The meat had been officially controlled...
Eight medical students ate for a test raw meat from a pig experimentally infected with sporocysts of Sarcocystis suihominis. The meat had been officially controlled before and was found free of Trichinella and qualified for consumption. 6 to 24 hours after the meal all persons suffered from acute clinical symptoms, above all diarrhoea and vomiting, coldness and sweating which decreased, however, within 12 to 24 hours. Clinical-chemical investigations made during this time suggested an acute infection combined with an exsiccosis; the specificity of the infection, however, could only be established by serological methods and by microscopical demonstration of sporocysts of the agent. The pathogenetic relations are discussed.
Topics: Adult; Antibodies; Creatinine; Dehydration; Diarrhea; Female; Food Microbiology; Humans; Male; Sarcocystis; Sarcocystosis; Urea; Vomiting
PubMed: 98426
DOI: No ID Found -
Berliner Und Munchener Tierarztliche... Apr 1978
Topics: Animals; Metamorphosis, Biological; Sarcocystis; Sarcocystosis; Swine; Swine Diseases
PubMed: 417714
DOI: No ID Found -
Zentralblatt Fur Bakteriologie,... Jan 1978
Topics: Animals; Cell Division; Humans; Kidney; Liver; Lung; Lymph Nodes; Sarcocystis; Spleen; Swine
PubMed: 416624
DOI: No ID Found -
Zentralblatt Fur Bakteriologie,... Sep 1977
Topics: Animals; Microscopy, Electron; Sarcocystis; Sarcocystosis; Swine
PubMed: 413280
DOI: No ID Found