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Journal of Clinical Microbiology Apr 1992Actinomadura madurae is an aerobic actinomycete which is best known worldwide as the cause of actinomycotic mycetomas. It has not previously been reported to have caused...
Actinomadura madurae is an aerobic actinomycete which is best known worldwide as the cause of actinomycotic mycetomas. It has not previously been reported to have caused invasive pulmonary or disseminated infection in humans. We describe an AIDS patient with opportunistic A. madurae-induced pneumonia and bacteremia. The isolate from the patient's blood was subjected to dilutional antimicrobial susceptibility tests with 12 antimicrobial agents and was found to have a wide spectrum of susceptibility. This unusual microorganism may be a cause of infections in severely immunosuppressed patients.
Topics: Acquired Immunodeficiency Syndrome; Actinomycetales; Actinomycetales Infections; Adult; Drug Resistance, Microbial; Fungemia; HIV-1; Humans; Male; Opportunistic Infections; Pneumonia
PubMed: 1572956
DOI: 10.1128/jcm.30.4.1008-1010.1992 -
The Journal of Antibiotics Apr 1991Maduropeptin, a complex of new macromolecular antitumor antibiotics, is a metabolite of Actinomadura madurae H710-49. The active components maduropeptins A1, A2 and B...
Maduropeptin, a complex of new macromolecular antitumor antibiotics, is a metabolite of Actinomadura madurae H710-49. The active components maduropeptins A1, A2 and B are acidic chromopeptides with MW of around 22,500 and composed of 14 types of amino acids and an unstable chromophore. The antibiotics are active in vitro against Gram-positive bacteria and highly cytotoxic to tumor cells. They produced significant prolongation of survival time of mice implanted with P388 leukemia and B16 melanoma.
Topics: Actinomyces; Amino Acids; Animals; Anti-Bacterial Agents; Antibiotics, Antineoplastic; Antimicrobial Cationic Peptides; Chromatography, High Pressure Liquid; Drug Stability; Enediynes; Female; Gram-Positive Bacteria; Leukemia P388; Male; Melanoma, Experimental; Mice; Molecular Weight; Peptide Biosynthesis; Peptides; Photochemistry
PubMed: 2032949
DOI: 10.7164/antibiotics.44.403 -
The Journal of Antibiotics Sep 1990A new antibacterial antibiotic, designated simaomicin alpha (LL-D42067 alpha) was isolated from the fermentation broth of an actinomycete strain. Based on cultural,...
A new antibacterial antibiotic, designated simaomicin alpha (LL-D42067 alpha) was isolated from the fermentation broth of an actinomycete strain. Based on cultural, physiological, morphological and chemical characteristics, culture LL-D42067 was identified as a new subspecies of Actinomadura madurae. Simaomicin alpha demonstrated potent activity against Gram-positive bacteria and was active in vivo against a variety of Eimeria species causing coccidiosis in chickens.
Topics: Animals; Anti-Bacterial Agents; Chickens; Coccidiosis; Coccidiostats; Fermentation; Gram-Positive Bacteria; Isoquinolines; Male; Molecular Structure; Nocardiaceae; Poultry Diseases; Soil Microbiology
PubMed: 2211367
DOI: 10.7164/antibiotics.43.1059 -
Journal of Clinical Microbiology Dec 1987Diagnosis of systemic nocardiosis has been hampered by difficulty in the rapid isolation and identification of the organism and by the frequent need for invasive...
Diagnosis of systemic nocardiosis has been hampered by difficulty in the rapid isolation and identification of the organism and by the frequent need for invasive biopsies. We show that a 55,000-Mr protein previously found in Nocardia asteroides is also present in N. brasiliensis and N. caviae culture filtrates. We also used the antigen in a dot blot assay to identify patients infected with N. brasiliensis. Sera from patients with mycetoma secondary to N. brasiliensis (n = 6) or Actinomadura madurae (n = 2) and 10 hospitalized controls were tested. Six (100%) of the sera from the patients with nocardial mycetoma had positive dot blots to purified antigen, whereas all 10 of the control sera were negative. Serum from one of two patients infected with A. madurae also reacted with the antigen. Thus, the 55-kilodalton protein appears to be produced by three medically important species of Nocardia and is useful when used in an immunoblot assay for identifying patients infected with these organisms.
Topics: Antibodies, Bacterial; Antigens, Bacterial; Bacterial Proteins; Electrophoresis, Polyacrylamide Gel; Humans; Immunoassay; Immunoglobulin G; Mycetoma; Nocardia; Nocardia Infections; Nocardia asteroides
PubMed: 3323223
DOI: 10.1128/jcm.25.12.2278-2280.1987 -
Applied Microbiology Jul 1969Thirteen red strains of Actinomadura (Nocardia) pelletieri and three of A. madurae were shown to produce prodigiosin-like pigments. Both of the two major pigments which...
Thirteen red strains of Actinomadura (Nocardia) pelletieri and three of A. madurae were shown to produce prodigiosin-like pigments. Both of the two major pigments which were observed on thin-layer chromatograms had R(F) values significantly greater than prodigiosin. The main pigment from A. madurae 953 was shown by mass and nuclear magnetic resonance spectroscopies to be nonylprodigiosin. The major pigment from A. pellitieri had a C(11)H(22) side chain in a ring form, but it was distinctly different from metacycloprodigiosin. "Prodiginine" was proposed as a name for the invariant aromatic portion of the prodigiosin structure.
Topics: Anti-Bacterial Agents; Chromatography; Chromatography, Thin Layer; Magnetic Resonance Spectroscopy; Nocardia; Pigments, Biological; Prodigiosin; Pyrroles; Spectrum Analysis
PubMed: 5803627
DOI: 10.1128/am.18.1.1-3.1969