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Molecules (Basel, Switzerland) Jun 2024L. possesses abundant germplasm resources and holds significant value in terms of ornamental, edible, and medicinal aspects. However, the quality characteristics vary...
L. possesses abundant germplasm resources and holds significant value in terms of ornamental, edible, and medicinal aspects. However, the quality characteristics vary significantly depending on different varieties. Selection of a high-quality variety with a characteristic aroma can increase the economic value of flowers. The analytic hierarchy process (AHP) is an effective decision-making method for comparing and evaluating multiple characteristic dimensions. By applying AHP, the aromatic character of 60 varieties of flowers were analyzed and evaluated in the present study. Headspace solid-phase microextraction gas chromatography-mass spectrometry (HS-SPME-GC-MS) was employed to identify volatile components in flowers. Thirteen volatile components were found to contribute to the aroma of flowers, which helps in assessing their potential applications in essential oil, aromatherapy, and medical treatment. These components include 2-phenylethanol, geraniol, linalool, nonanal, decanal, (E)--ocimene, α-farnesene, indole, nerolidol, 3-furanmethanol, 3-carene, benzaldehyde and benzenemethanol. The varieties with better aromatic potential can be selected from a large amount of data using an AHP model. This study provides a comprehensive understanding of the characteristics of the aroma components in flowers, offers guidance for breeding, and enhances the economic value of flowers.
Topics: Gas Chromatography-Mass Spectrometry; Volatile Organic Compounds; Solid Phase Microextraction; Flowers; Odorants; Acyclic Monoterpenes; Oils, Volatile; Sesquiterpenes; Phenylethyl Alcohol; Alkenes; Indoles
PubMed: 38893586
DOI: 10.3390/molecules29112712 -
ISME Communications Jan 2024The performance of sequence variant resolution analytic tools for metabarcoding has not yet been adequately benchmarked for high-diversity environmental samples. We...
The performance of sequence variant resolution analytic tools for metabarcoding has not yet been adequately benchmarked for high-diversity environmental samples. We therefore evaluated the sequence variant tools DADA2, Deblur, Swarm, and UNOISE, using high-diversity seafloor samples, resulting in comparisons of 1800 sequence variant tables. The evaluation was based on 30 sediment grab samples, for which 3 replica samples were collected. Each replica sample was extracted using 5 common DNA extraction kits, resulting in 450 DNA extracts which were 16S rRNA gene sequenced (V3-V4), using Illumina. Assessments included variation across replica samples, extraction kits, and denoising methods, in addition to applying prior knowledge about alpha diversity correlations toward the cosmopolitan marine archaeon with high diversity and the sulfide oxidizing with low diversity DADA2 displayed the highest variance between replicates (Manhattan distance 1.14), while Swarm showed the lowest variance (Manhattan distance 0.93). For the analysis based on prior biological knowledge, UNOISE displayed the highest alpha diversity (Simpson's D) correlation toward (Spearman rho = 0.85), while DADA2 showed the lowest (Spearman rho = 0.10). Deblur completely eliminated from the dataset. For , on the other hand, all the methods showed comparable results. In conclusion, our evaluations show that Swarm and UNOISE performed better than DADA2 and Deblur for high-diversity seafloor samples.
PubMed: 38873028
DOI: 10.1093/ismeco/ycae071 -
Frontiers in Bioengineering and... 2024Pharmaceutical manufacturing is reliant upon bioprocessing approaches to generate the range of therapeutic products that are available today. The high cost of...
Pharmaceutical manufacturing is reliant upon bioprocessing approaches to generate the range of therapeutic products that are available today. The high cost of production, susceptibility to process failure, and requirement to achieve consistent, high-quality product means that process monitoring is paramount during manufacturing. Process analytic technologies (PAT) are key to ensuring high quality product is produced at all stages of development. Spectroscopy-based technologies are well suited as PAT approaches as they are non-destructive and require minimum sample preparation. This study explored the use of a novel attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy platform, which utilises disposable internal reflection elements (IREs), as a method of upstream bioprocess monitoring. The platform was used to characterise organism health and to quantify cellular metabolites in growth media using quantification models to predict glucose and lactic acid levels both singularly and combined. Separation of the healthy and nutrient deficient cells within PC space was clearly apparent, indicating this technique could be used to characterise these classes. For the metabolite quantification, the binary models yielded values of 0.969 for glucose, 0.976 for lactic acid. When quantifying the metabolites in tandem using a multi-output partial least squares model, the corresponding value was 0.980. This initial study highlights the suitability of the platform for bioprocess monitoring and paves the way for future in-line developments.
PubMed: 38863496
DOI: 10.3389/fbioe.2024.1349473 -
Heliyon Jun 2024An analytic design of a prototype filter for M-channel maximally decimated cosine-modulated Near Perfect Reconstruction (NPR) filter banks is proposed in this work. The...
An analytic design of a prototype filter for M-channel maximally decimated cosine-modulated Near Perfect Reconstruction (NPR) filter banks is proposed in this work. The prototype filter is created using the least-square (CLS) method with weighted constraints, which is one-dimensional and requires single-parameter optimization. Compared to existing approaches, this suggested method achieves rapid convergence by analytically determining the optimal step size, ensuring the 3 dB cutoff frequency at π/2 M. The simulation results for design examples outperform the techniques in the available literature in terms of amplitude and aliasing distortion, reaching distortion around 2.4489 × 10 and 3.4907 × 10, respectively. This optimization algorithm's usefulness is further demonstrated with the sub-band coding of ECG signals Implementing optimal prototype filters has tangible real-world effects, especially in critical sectors like healthcare and communications, improving diagnostics accuracy, data transmission efficiency, and overall performance
PubMed: 38841468
DOI: 10.1016/j.heliyon.2024.e31912 -
European Journal of Pharmaceutical... Aug 2024Bioequivalence determinations for locally acting dermatology drug products rely on assessing product sameness thru physicochemical composition and structure comparison,...
Bioequivalence determinations for locally acting dermatology drug products rely on assessing product sameness thru physicochemical composition and structure comparison, comparing the concentration of the active ingredient at the putative site of action, or comparing the clinical performance of the test (would-be generic) and reference products. Topical product action on cutaneous disease may be confounded by the action of excipients and are also subject to the inherent variability of how product may interact with the skin, including thermodynamic factors such as evaporation, spreadability, and interaction with the local environment such as heat and light and skin moisture.
Topics: Humans; Administration, Cutaneous; Dermatologic Agents; Excipients; Skin; Skin Diseases; Therapeutic Equivalency
PubMed: 38797441
DOI: 10.1016/j.ejps.2024.106815 -
Langmuir : the ACS Journal of Surfaces... Jun 2024Hysteresis is observed commonly in sorption isotherms of porous materials. Still, there has so far been no unified approach that can both model hysteresis and assess its...
Hysteresis is observed commonly in sorption isotherms of porous materials. Still, there has so far been no unified approach that can both model hysteresis and assess its underlying energetics. Standard approaches, such as capillary condensation and isotherms based on interfacial equations of state, have not proved to be up to the task. Here, we show that a statistical thermodynamic approach can achieve the following needs simultaneously: (i) showing why adsorption and desorption transitions may be sharp yet continuous; (ii) providing a simple (analytic) isotherm equation for hysteresis branches; (iii) clarifying the energetics underlying sorption hysteresis; and (iv) providing macroscopic and nanoscopic perspectives to understanding hysteresis. This approach identifies the two pairs of parameters (determinable by fitting experimental data) that are required to describe the hysteresis: the free energy per molecule within the pore clusters and the cluster size in the pores. The present paper focuses on providing mechanistic insights to IUPAC hysteresis types H1, H2(a), and H2(b) and can also be applied to the isotherm types IV and V.
PubMed: 38780491
DOI: 10.1021/acs.langmuir.4c00606 -
Joint Diseases and Related Surgery Mar 2024This study aimed to investigate whether adding tissue samples directly into thioglycolate (TG) broth yielded a greater number of anaerobic organisms than freshly sampled...
OBJECTIVES
This study aimed to investigate whether adding tissue samples directly into thioglycolate (TG) broth yielded a greater number of anaerobic organisms than freshly sampled tissue in suspected hip and knee prosthetic joint infections (PJIs).
PATIENTS AND METHODS
Between January 2017 and December 2020, a total of 90 patients (46 males, 44 females; median age: 71.7 years; range, 50.8 and 87.8 years) who underwent revision hip or knee arthroplasty were included. Intraoperative samples were taken, with five placed in TG broth and five in standard containers (PC) with subsequent aerobic and anaerobic culturing conducted. Demographic and baseline data of the patients were recorded. The primary outcome was positive bacterial growth from a PJI specimen inoculated directly into TG broth at the time of collection or standard PJI specimen processing. Secondary outcomes investigated were the presence of Cutibacterium acnes (C. acnes) and the curative success of revision procedure.
RESULTS
A total of 900 samples (450 PC and 450 TG) were taken from 90 revision arthroplasty patients (47 knees and 43 hips). There was no statistically significant difference in the number of positive bacterial growth samples between TG broth and standard processing (p=0.742). This was consistent with subgroup analysis analyzing C. acnes (p=0.666).
CONCLUSION
In hip and knee arthroplasty, there is no benefit in substituting or adding TG broth as a culture medium to better identify both general bacterial species and C. acnes infections specifically. However, the use of TG may be useful in confirming a true positive result for infection.
Topics: Humans; Prosthesis-Related Infections; Female; Male; Aged; Middle Aged; Aged, 80 and over; Arthroplasty, Replacement, Knee; Arthroplasty, Replacement, Hip; Thioglycolates; Knee Prosthesis; Culture Media; Reoperation; Hip Prosthesis; Specimen Handling; Retrospective Studies
PubMed: 38727108
DOI: 10.52312/jdrs.2024.1364 -
Biosensors Mar 2024Covalent organic frameworks (COFs) are porous crystals that have high designability and great potential in designing, encapsulating, and immobilizing nanozymes. COF... (Review)
Review
Covalent organic frameworks (COFs) are porous crystals that have high designability and great potential in designing, encapsulating, and immobilizing nanozymes. COF nanozymes have also attracted extensive attention in analyte sensing and detection because of their abundant active sites, high enzyme-carrying capacity, and significantly improved stability. In this paper, we classify COF nanozymes into three types and review their characteristics and advantages. Then, the synthesis methods of these COF nanozymes are introduced, and their performances are compared in a list. Finally, the applications of COF nanozymes in environmental analysis, food analysis, medicine analysis, disease diagnosis, and treatment are reviewed. Furthermore, we also discuss the application prospects of COF nanozymes and the challenges they face.
Topics: Metal-Organic Frameworks; Biosensing Techniques; Nanostructures; Chemistry, Analytic; Food Analysis; Chemistry Techniques, Analytical
PubMed: 38667156
DOI: 10.3390/bios14040163 -
JACS Au Apr 2024We investigated room-temperature metal and ligand K-edge X-ray absorption (XAS) spectra of a complete redox series of cubane-type iron-sulfur clusters. The Fe K-edge...
We investigated room-temperature metal and ligand K-edge X-ray absorption (XAS) spectra of a complete redox series of cubane-type iron-sulfur clusters. The Fe K-edge position provides a qualitative but convenient alternative to the traditional spectroscopic descriptors used to identify oxidation states in these systems, which we demonstrate by providing a calibration curve based on two analytic methods. Furthermore, high energy resolution fluorescence detected XAS (HERFD-XAS) at the S K-edge was used to measure Fe-S bond covalencies and record their variation with the average valence of the Fe atoms. While the Fe-S(thiolate) covalency evolves linearly, gaining 11 ± 0.4% per bond and hole, the Fe-S(μ) covalency evolves asystematically, reflecting changes in the magnetic exchange mechanism. A strong discontinuity manifested for superoxidation to the all-ferric state, distinguishing its electronic structure and its potential (bio)chemical role from those of its redox congeners. We highlight the functional implications of these trends for the reactivity of iron-sulfur cubanes.
PubMed: 38665672
DOI: 10.1021/jacsau.4c00213 -
Global Epidemiology Jun 2024Evidence triangulation may help identify the impact of study design elements on study findings and to tease out biased results when evaluating potential causal...
Evidence triangulation may help identify the impact of study design elements on study findings and to tease out biased results when evaluating potential causal relationships; however, methods for triangulating epidemiologic evidence are evolving and have not been standardized. Building upon key principles of epidemiologic evidence triangulation and risk of bias assessment, and responding to the National Academies of Sciences, Engineering, and Medicine (NASEM) call for applied triangulation examples, the objective of this manuscript is to propose a triangulation framework and to apply it as an illustrative example to epidemiologic studies examining the possible relationship between occupational formaldehyde exposure and risk of myeloid leukemias (ML) including acute (AML) and chronic (CML) types. A nine-component triangulation framework for epidemiological evidence was developed incorporating study quality and ROB guidance from various federal health agencies (i.e., US EPA TSCA and NTP OHAT). Several components of the triangulation framework also drew from widely used epidemiological analytic tools such as stratified meta-analysis and sensitivity analysis. Regarding the applied example, fourteen studies were identified and assessed using the following primary study quality domains to explore potential key sources of bias: 1) study design and analysis; 2) study participation; 3) exposure assessment; 4) outcome assessment; and 5) potential confounding. Across studies, methodological limitations possibly contributing to biased results were observed within most domains. Interestingly, results from one study - often providing the largest and least-precise relative risk estimates, likely reflecting study biases, deviated from most primary study findings indicating no such associations. Triangulation of epidemiological evidence appears to be helpful in exploring inconsistent results for the identification of study results possibly reflecting various biases. Nonetheless, triangulation methodologies require additional development and application to real-world examples to enhance objectivity and reproducibility.
PubMed: 38659700
DOI: 10.1016/j.gloepi.2024.100143