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The Journal of Biological Chemistry Aug 1961
Topics: 4-Aminobenzoic Acid; Acetylation; Albumins; Arsanilic Acid; Arsenic; Serum Albumin; Serum Albumin, Bovine
PubMed: 13764174
DOI: No ID Found -
The Journal of Biological Chemistry Apr 1960
Topics: Arsanilic Acid; Proteins; Spectrophotometry
PubMed: 13836501
DOI: No ID Found -
Immunology Apr 1959The Ouchterlony patterns of fifteen grass pollen extracts (against rabbit antisera to the pollens of Cocksfoot— and Timothy—) are compared with skin reactivities in...
The Ouchterlony patterns of fifteen grass pollen extracts (against rabbit antisera to the pollens of Cocksfoot— and Timothy—) are compared with skin reactivities in allergic subjects of this country and with quantitative assays in terms of a heat labile component of Cocksfoot referred to as antigen A. Problems of standardization in general and of the Noon unit in particular are discussed. Ten indigenous pollens are shown to give positive skin responses in all the pollen sensitive subjects tested, except in one with a most unusual selective specificity for Timothy alone. All indigenous pollens examined contain substances cross-reacting with antigen A, while the four `foreign' pollens, which give small or no skin responses, do not share the A-group specificity. Nevertheless A-content cannot be equated with skin reactivity. British pollens have numerous antigenic determinants in common other than the A-group specificity, although the occurrence of completely identical antigens in different pollens is unlikely. Complications in the interpretation of Ouchterlony patterns of related natural products are discussed from a purely serological point of view with the help of azoprotein models. Evidence is presented for the occurrence of what may be called . It is suggested that antibodies specific purely for small haptenic groups such as arsanilic acid are probably never produced. From gel diffusion tests on numerous bleedings from rabbits it seems that prolonged immunization does not lead to the formation of less specific sera as is usually suggested. On the contrary, more and more precisely fitting antibodies appear to be produced to an ever-increasing number of related antigens and minor impurities, probably to more determinants on one and the same molecule or even to new permutations of adjacent determinants on the same molecule. The need is stressed for more precise definitions of the terms and .
Topics: Allergens; Antigen-Antibody Reactions; Humans; Hypersensitivity; Poaceae; Pollen
PubMed: 13653735
DOI: No ID Found -
The Journal of Experimental Medicine Nov 1958Conjugates made by coupling diazotized arsanilic acid with one or another of a variety of proteins regularly brought about the complete regression of established 6C3HED...
Effects of arsenic-azoproteins on mouse lymphoma cells in vivo; with observations on the effects of other anti-lymphoma agents, and on the susceptibility to these effects of lymphoma cells of various types.
Conjugates made by coupling diazotized arsanilic acid with one or another of a variety of proteins regularly brought about the complete regression of established 6C3HED lymphomas in living mice without perceptibly harming the latter, while untreated control animals regularly died with lymphomatosis. Histologic studies made plain that the lymphoma cells promptly die in mice treated with the arsenic-azoproteins, while those in untreated control animals continue to proliferate. Various inorganic and organic arsenicals (including arsanilic acid and 4-arsonophenyldiazotate) were essentially devoid of effect on the lymphoma cells in vivo, and this proved true as well of the proteins employed (serum albumins and globulins procured from several species, casein, and ovalbumin). Mixtures of arsanilic acid and the several proteins, various sulfur-azoproteins, and a number of other substances-viz., amethopterin, chlorambucil, 6-mercaptopurine, 8-azaguanine, azaserine, 6-azauracil, 5-fluorouracil, thioTEPA, and DON, each given in maximal tolerated amounts-also failed to influence notably the course of established 6C3HED lymphomas in vivo. Although readily overcoming Lymphoma E9514 cells growing in the subcutaneous tissues of susceptible mice, the arsenic-azoproteins had little or no effect once these cells had reached the livers and spleens of susceptible hosts. Furthermore the arsenic-azoproteins had little or no effect in vivo on the cells of Lymphoma AKRL1, L1210, and L4946. The findings were considered in relationship to the respective susceptibilities of several types of lymphoma cells to other anti-lymphoma agents-notably guinea pig serum, immune serums prepared in rabbits with mouse lymphoma cells as antigens, and a variety of chemical compounds. Taken together, the observations provide proof that lymphoma cells of various types, although resembling one another quite closely in growth characteristics following transplantation in susceptible hosts, and in morphology as disclosed by ordinary microscopy, differ notably in susceptibility to the effects of the several anti-lymphoma agents.
Topics: Animals; Antineoplastic Agents; Arsenic; Arsenicals; Fluorouracil; Guinea Pigs; Lymphoma; Mice; Neoplasms, Experimental
PubMed: 13587850
DOI: 10.1084/jem.108.5.665 -
Canadian Medical Association Journal Sep 1953
Topics: Arsanilic Acid; Balantidiasis; Dysentery; Humans
PubMed: 13082481
DOI: No ID Found -
California Medicine Apr 1952Among the less commonly recognized clinical manifestations of intestinal and hepatic amebiasis are vague abdominal distress in the absence of diarrhea, symptoms like...
Among the less commonly recognized clinical manifestations of intestinal and hepatic amebiasis are vague abdominal distress in the absence of diarrhea, symptoms like those of peptic ulcer, and symptoms of a kind that may be ascribed to psychoneurosis. Hepatic amebiasis may be confused with other diseases affecting areas above or below the right diaphragm, such as cholecystitis, viral hepatitis, pneumonia or pleurisy. Adequate therapy in every case must include a course of a drug effective against hepatic involvement (chloroquine or emetine) and a drug effective against intestinal involvement (Diodoquin, Milibis, or carbarsone). Even in the absence of positive results of stool examinations, a course of antiamebic therapy is always justified as a diagnostic and therapeutic measure.
Topics: Amebiasis; Arsanilic Acid; Chloroquine; Cholecystitis; Diarrhea; Dysentery, Amebic; Emetine; Humans; Iodoquinol; Liver Abscess, Amebic; Pleurisy
PubMed: 14925826
DOI: No ID Found -
The Cornell Veterinarian Jul 1951
Topics: Animals; Arsanilic Acid; Arsenic; Bacitracin; Disease Outbreaks; Dysentery; Enteritis; Sulfamethazine; Sulfathiazoles; Swine; Therapies, Investigational
PubMed: 14849145
DOI: No ID Found -
The Indian Medical Gazette Feb 1950
Topics: Arsanilic Acid; Arsenic; Arsenicals
PubMed: 15415143
DOI: No ID Found -
The Journal of Experimental Medicine Apr 1924From the experiments which have been reported, it follows that animals sensitized with one azoprotein react not only to the antigen used for the sensitization, but also...
From the experiments which have been reported, it follows that animals sensitized with one azoprotein react not only to the antigen used for the sensitization, but also to other azoproteins made up from the same simple azo-compounds and another protein. Although the specificity of the reaction has not yet been tested with various azo-components, its actual existence can reasonably be assumed on the basis of the phenomena observed in precipitation reactions. The sensitization is brought about with less facility than sensitization against the usual antigens and the effects are not uniform. Still, after sufficient treatment, 40 per cent of the animals succumbed with typical anaphylactic symptoms, mostly within a short time while 16 per cent showed severe symptoms. The experiments show that it is possible to make animals hypersensitive against a simple chemical group like para-arsanilic acid, and from this point of view connection would seem to be established with the phenomena of drug allergy in human beings. There is an essential difference, however, in that the sensitized animals did not react on injections of simple compounds such as para-amino-phenyl-arsanilic acid and phenyl-4-arsonic-acid-azo-tyrosine uncombined with protein. It remains to be determined whether under changed conditions positive results in this direction can be obtained. For this purpose it seems advisable to make experiments with isolated organs, according to the method of Schultz and Dale. While the simple substances failed to elicit direct reactions, they protected (as was foreseen by Doerr) against a subsequent injection of the active antigen. Similar compounds not containing the arsanilic acid group were considerably less active. The phenomenon is comparable to the inhibition of precipitin reactions already described. Considering the protection as a condition of antianaphylaxis, one would suppose that the simple substances mentioned are fixed by the cells in which the anaphylactic reaction takes place. It may be concluded that: 1. Animals can be sensitized through injections of one azoprotein-protein combined with diazotized para-arsanilic acid-against another compound containing the same azo-component but a different protein. 2. Injections of related simple compounds, as for instance para-arsanilic acid and phenyl-4-arsonic-acid-azo-tyrosine did not cause shock in the sensitized animals under the conditions of the present experiments. 3. The simple compounds mentioned and other related substances as well protect against the anaphylactic action of azoprotein, by inducing a state of antianaphylaxis.
PubMed: 19868873
DOI: 10.1084/jem.39.5.631