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Scientific Reports Jun 2023The aim of this study was to evaluate the effect of Lactobacillus delbrueckii subsp. lactis (L.del) on vaginal microbiota (VM) dysbiosis and vaginal radiation injury in... (Randomized Controlled Trial)
Randomized Controlled Trial
The aim of this study was to evaluate the effect of Lactobacillus delbrueckii subsp. lactis (L.del) on vaginal microbiota (VM) dysbiosis and vaginal radiation injury in gynecologic cancer patients. The inhibitory effects of L.del on cervical cancer cells were also studied in vitro. Gynecologic cancer patients receiving radiotherapy were randomized into control and L.del intervention groups. The control group received radiotherapy, while the intervention group received radiotherapy and L.del intervention (1 capsule/day placed into the deep vagina from the first day of radiotherapy until the end of treatment). Vaginal swab samples were collected on the first day pre-treatment and the last day post-treatment. DNA from 54 patients was extracted and assessed by the 16S rRNA sequencing method. Radiotherapy resulted in vaginal microbiome dysbiosis characterized by increased phylogenetic diversity and increased abundance of Brevundimonas, Streptococcus and Prevotella, but a decreased abundance of Lactobacillus. Level 2 vaginal radiation injury was positively associated with the abundance of Brevundimonas and gram-negative non-fermenting bacteria. Administration of L.del attenuated the reduction of Lactobacillus while also inhibiting the abundance of Streptococcus and Prevotella, thereby ameliorating radiotherapy-related vaginal microbiota dysbiosis. CLD inhibited the in vitro proliferation of SiHa cells by altering the expression of BCL2, HPV16-E6, HPV16-E7, IL6, MAP7, BAX, Caspase-3, Caspase-9 and LTF. In conclusion, L. del application can alleviate radiation-induced vaginal dysbiosis and restore Lactobacillus dominance of the vaginal microbiome. Moreover, CLD was found to inhibit cell growth and promote the apoptosis of SiHa cells in vitro. The registration number for this clinical trial is ChiCTR1900021784.
Topics: Female; Humans; Dysbiosis; RNA, Ribosomal, 16S; Phylogeny; Vagina; Lactobacillus; Streptococcus; Neoplasms
PubMed: 37344615
DOI: 10.1038/s41598-023-37241-7 -
Heliyon Jun 2023The roles of plant growth-promoting rhizobacteria in promoting plant growth and soil health, including alteration in plant metabolism and production of phytohormones...
The roles of plant growth-promoting rhizobacteria in promoting plant growth and soil health, including alteration in plant metabolism and production of phytohormones such as indole-3-acetic acid (IAA) and the enzyme 1-aminocyclopropane-1-carboxylate (ACC) deaminase, are indisputable. This study aimed to isolate and characterize beneficial bacteria isolated from the rhizosphere of pineapple from distinct stress-inducing habitats, including water excess-, herbicide-over-treated-, and pathogen-infected areas at PT Great Giant Foods located in Lampung, Indonesia. The isolated bacteria were screened based on IAA production and ACC deaminase activities. Six selected isolates produced IAA with concentrations of up to 36.93 mgL. The highest value belongs to sp. NCTB5I, followed by sp. CHTB 2C (13.13 mgL) and sp. CHTB 5B (6.65 mgL). All isolates were detected with ACC deaminase activities with sp. CHTJ 5H consuming 88% of ACC over 24 h, the highest among all. sp. CHTB 2C was detected with the highest ACC deaminase activity with the value of 13,370 nm α-ketobutyrate mgh. In another experiment, it was revealed that all selected isolates promote soybean growth. These bacteria are potential to be developed in the future as bioagents to promote plant growth, especially under stressful environmental conditions.
PubMed: 37292365
DOI: 10.1016/j.heliyon.2023.e16306 -
Microbiology Resource Announcements Jul 2023We report the genome sequence of sp. strain NIBR11. Strain NIBR11 was isolated from algae collected from the Nakdong River. The assembled contig contains 3,123 coding...
We report the genome sequence of sp. strain NIBR11. Strain NIBR11 was isolated from algae collected from the Nakdong River. The assembled contig contains 3,123 coding sequences (CDSs), 6 rRNA genes, 48 tRNA genes, 1,623 genes for hypothetical proteins, and 109 genes for proteins with putative functions.
PubMed: 37284755
DOI: 10.1128/mra.00087-23 -
Frontiers in Microbiology 2023This study aimed to explore the differences between Eucommiae cortex (EC) endophytic bacteria from different origins and their effects on the active ingredients of EC.
OBJECTIVE
This study aimed to explore the differences between Eucommiae cortex (EC) endophytic bacteria from different origins and their effects on the active ingredients of EC.
METHODS
A total of 10 samples of Oliv. () bark were collected from each of the following four regions, namely, Zunyi in Guizhou (GZ), Baokang in Hubei (HUB), Cili in Hunan (HUN), and Loyang in Shaanxi (SX). Subsequently, the contents of the main active ingredients of EC were determined by ultra-performance liquid chromatography (UPLC), and the endophytic bacteria of EC were detected by 16S rRNA sequencing. The relationship between the dominant endophytic bacteria and the active ingredients was investigated by correlation analysis.
RESULTS
A total of 4,551 different operational taxonomic units (OTUs) were delineated in the four groups of samples, of which 585, 439, 957, and 684 genera were annotated from GZ, HUB, HUN, and SX, respectively. The richness and diversity of endophytic bacteria from different origins were ranked as HUN > SX > GZ or HUB. The analysis demonstrated that there was no significant correlation between the diversity and richness of endophytic bacteria in EC and its active ingredients. Nevertheless, notable variations in the community structures of endophytic bacteria were observed across different origins, and they had a considerable impact on certain active ingredients in EC. and were the dominant genera. Characteristic bacteria of different origins could be clearly distinguished. Simultaneous, significant correlations had been identified between some characteristic endophytic bacteria derived from different origins and active ingredients of EC. For example, , a characteristic bacterium from GZ, showed a significant positive correlation with pinoresinol diglucoside. and , two characteristic bacteria from HUB, exhibited significant positive correlations with geniposidic acid. , a characteristic bacterium from HUN, demonstrated a significant positive correlation with geniposide. , a characteristic bacterium from SX, displayed a significant positive correlation with pinoresinol diglucoside.
CONCLUSION
There was a complex correlation between EC endophytic bacteria and active ingredient content, while EC endophytic bacteria from different origins had significant differences at the genus level.
PubMed: 37266017
DOI: 10.3389/fmicb.2023.1164674 -
Frontiers in Microbiology 2023One of the major variables affecting yield of the mushroom is the casing layer, which directly affects the productivity and mass. Here, volatile organic compounds were...
One of the major variables affecting yield of the mushroom is the casing layer, which directly affects the productivity and mass. Here, volatile organic compounds were extracted by headspace solid-phase microextraction and high-throughput sequencing was used to analyze the microbial community diversity. The relationship between mushroom yield at different cropping stages and the contents of volatile organic compounds and microorganisms in three different casing layers: peat, peat + soil and soil were systematically evaluated. The result shows that Benzaldehyde and (E)-2-octenal which stimulate yield, obviously increased as mushrooms grew, while 3-octanone, which inhibits yield, decreased over time in all three casing layers. However, there was not a strong correlation between the concentration of volatile compounds and yield. In addition, more than 3,000 bacterial operational taxonomic units (OTUs) by performing high throughput sequencing of the microbes were obtained in the three casing layers. Interestingly, the microbial community compositions were very similar between the three casing layers at a later cropping stage, but the community richness varied significantly in different casing layers and at different cropping stages. At the phylum level, the communities had similar structures but were quantitively very different, and this was even more obvious at the genus level. Principal component analysis revealed significant alterations in microbial community structure in different casing layers. , and were the dominant genera at cropping stage 1, and the stage 3 were abundant in , and , which was positively correlated with yield, while the abundance of at stage 1 and and at stage 3 was negatively correlated with yield. These results provide a guide for the development and agricultural application of microbial agents for yield improvement in the production of .
PubMed: 37266010
DOI: 10.3389/fmicb.2023.1154903 -
Environmental Science and Pollution... Jun 2023The aim of the study was to determine totoxicity of bisphenol A (BPA) and its derivatives (bisphenol S (BPS), bisphenol F (BPF), and tetrabromobisphenol A (TBBPA)) due...
The aim of the study was to determine totoxicity of bisphenol A (BPA) and its derivatives (bisphenol S (BPS), bisphenol F (BPF), and tetrabromobisphenol A (TBBPA)) due to its high accumulation in environment. The performed analysis revealed the toxicity of the BPA, BPF, and BPS against Kurthia gibsoni, Microbacterium sp., and Brevundimonas diminuta as the most sensitive, reaching microbial toxic concentrations in the range of 0.018-0.031 mg ∙ L. Moreover, the genotoxicity assay shows the ability of all tested compounds to increase in the β-galactosidase level at the concentration range 7.81-500 µM (in Escherichia coli, PQ37). In turn, the matbolic activation of tested bishpenols has caused the enhacement of the genotoxicity and cytotoxicity effect. Interestingely, the highest phytotoxicity effect was pointed for BPA and TBBPA at the concentrations of 10 mg ∙ L and 50 mg ∙ L, which cause the inhibition of root growth by 58% and 45%, respectively (especially for S. alba and S. saccharatum). Furthermore, the cytotoxicity analyses show the ability of BPA, BPS, and TBBPA to significantly decrease the metabolic activity of human keratynoctes in vitro after 24 h of treatment at the micromolar concentrations. Simialry, the impact of the certain bisphenols on proliferation-, apoptosis-, and inflammation-related mRNA expression was shown in tested cell line. Summarizing, the presented results have proved that BPA and its derrivatives are able to show high negative effect on certain living orgnisms such as bacteria, plants, and human cells, which is strict related to pro-apoptotic and genotoxic mechanism of action.
Topics: Humans; Cell Line; Benzhydryl Compounds; Phenols
PubMed: 37213006
DOI: 10.1007/s11356-023-27747-y -
BMC Microbiology May 2023Arsenic (As) with various chemical forms, including inorganic arsenic and organic arsenic, is the most prevalent water and environmental toxin. This metalloid occurs...
BACKGROUND
Arsenic (As) with various chemical forms, including inorganic arsenic and organic arsenic, is the most prevalent water and environmental toxin. This metalloid occurs worldwide and many of its forms, especially arsenite [As(III)], cause various diseases including cancer. Organification of arsenite is an effective way for organisms to cope with arsenic toxicity. Microbial communities are vital contributors to the global arsenic biocycle and represent a promising way to reduce arsenite toxicity.
METHODS
Brevundimonas sp. M20 with arsenite and roxarsone resistance was isolated from aquaculture sewage. The arsHRNBC cluster and the metRFHH operon of M20 were identified by sequencing. The gene encoding ArsR/methyltransferase fusion protein, arsR, was amplified and expressed in Escherichia coli BL21 (DE3), and this strain showed resistance to arsenic in the present of 0.25-6 mM As(III), aresenate, or pentavalent roxarsone. The methylation activity and regulatory action of ArsR were analyzed using Discovery Studio 2.0, and its functions were confirmed by methyltransferase activity analysis and electrophoretic mobility shift assays.
RESULTS
The minimum inhibitory concentration of the roxarsone resistant strain Brevundimonas sp. M20 to arsenite was 4.5 mM. A 3,011-bp arsenite resistance ars cluster arsHRNBC and a 5649-bp methionine biosynthesis met operon were found on the 3.315-Mb chromosome. Functional prediction analyses suggested that ArsR is a difunctional protein with transcriptional regulation and methyltransferase activities. Expression of ArsR in E. coli increased its arsenite resistance to 1.5 mM. The arsenite methylation activity of ArsR and its ability to bind to its own gene promoter were confirmed. The As(III)-binding site (ABS) and S-adenosylmethionine-binding motif are responsible for the difunctional characteristic of ArsR.
CONCLUSIONS
We conclude that ArsR promotes arsenite methylation and is able to bind to its own promoter region to regulate transcription. This difunctional characteristic directly connects methionine and arsenic metabolism. Our findings contribute important new knowledge about microbial arsenic resistance and detoxification. Future work should further explore how ArsR regulates the met operon and the ars cluster.
Topics: Arsenic; Arsenites; Base Sequence; Escherichia coli; Methylation; Roxarsone; Bacterial Proteins; Arsenicals; Operon; Methyltransferases; Methionine; Gene Expression Regulation, Bacterial; Trans-Activators
PubMed: 37193944
DOI: 10.1186/s12866-023-02876-z -
Polymers Apr 2023Polystyrene (PS) and microplastic production pose persistent threats to the ecosystem. Even the pristine Antarctic, which is widely believed to be pollution-free, was...
Polystyrene (PS) and microplastic production pose persistent threats to the ecosystem. Even the pristine Antarctic, which is widely believed to be pollution-free, was also affected by the presence of microplastics. Therefore, it is important to comprehend the extent to which biological agents such as bacteria utilise PS microplastics as a carbon source. In this study, four soil bacteria from Greenwich Island, Antarctica, were isolated. A preliminary screening of the isolates for PS microplastics utilisation in the Bushnell Haas broth was conducted with the shake-flask method. The isolate AYDL1 identified as sp. was found to be the most efficient in utilising PS microplastics. An assay on PS microplastics utilisation showed that the strain AYDL1 tolerated PS microplastics well under prolonged exposure with a weight loss percentage of 19.3% after the first interval (10 days of incubation). Infrared spectroscopy showed that the bacteria altered the chemical structure of PS while a deformation of the surface morphology of PS microplastics was observed via scanning electron microscopy after being incubated for 40 days. The obtained results may essentially indicate the utilisation of liable polymer additives or "leachates" and thus, validate the mechanistic approach for a typical initiation process of PS microplastics biodeterioration by the bacteria (AYDL1)-the biotic process.
PubMed: 37111988
DOI: 10.3390/polym15081841 -
Microorganisms Apr 2023is broadly distributed in terrestrial and aquatic environments and has various biological activities. In this study, we found that exhibited nematicidal activity...
is broadly distributed in terrestrial and aquatic environments and has various biological activities. In this study, we found that exhibited nematicidal activity against the plant root-knot nematode, . A total of 42 volatile organic compounds (VOCs) from were identified using gas chromatography-mass spectrometry (GC-MS). The nematicidal activity of the 10 main VOCs was tested against . Butyl butanoate (4 µL) caused the mortality of 80.13% of after 4 h. The nematicidal activity of an additional 38 butyl-butyrate-like volatile esters was also investigated. Of these, seven had strong nematicidal activity against , five of which showed egg-hatching inhibitory activity. This study is the first to report that butyl butanoate, ethyl 2-methylbutanoate, ethyl 4-methylpentanoate, ethyl pent-4-enoate, and methyl undecanoate have nematicidal activity against . The results indicated that could serve as a candidate microorganism for the biocontrol of plant root-knot nematodes, showing that volatile esters have great potential as nematicides.
PubMed: 37110389
DOI: 10.3390/microorganisms11040966 -
Microbiology Spectrum Jun 2023To increase knowledge on pathogens, we conducted in-depth genomic and phenotypic characterization of a strain isolated from the cerebrospinal fluid of a patient...
To increase knowledge on pathogens, we conducted in-depth genomic and phenotypic characterization of a strain isolated from the cerebrospinal fluid of a patient admitted in a neonatal intensive care unit. The strain was identified as a member of the genus based on Vitek 2 system results and 16S rRNA gene sequencing and presented a multidrug resistance profile (MDR). Several molecular and biochemical tests were used to characterize and identify the species for in-depth results. The draft genome assembly of the isolate has a total length of 3,261,074 bp and a G+C of 66.86%, similar to other species of the genus. Multilocus sequence analysis, Type (Strain) Genome Server, digital DNA-DNA hybridization, and average nucleotide identity confirmed that the sp. studied represents a distinct species, for which we propose the name sp. nov. analysis detected antimicrobial resistance genes (AMRGs) mediating resistance to β-lactams (, , and ) and aminoglycosides [, , , and ]. We also found AMRGs encoding the AcrAB efflux pump that confers resistance to a broad spectrum of antibiotics. Colistin and quinolone resistance can be attributed to mutation in and/or and GyrA/GyrB, respectively. The sp. nov. genome contained copies of type IV secretion system (T4SS)-type integrative and conjugative elements (ICEs); integrative mobilizable elements (IME); and Tn-type and IS, IS, IS, and IS families, suggesting an important role in the development and dissemination of antibiotic resistance. The isolate presented a range of virulence-associated genes related to biofilm formation, adhesion, and invasion that can be relevant for its pathogenicity. Our findings provide a wealth of data to hinder the transmission of MDR and highlight the need for monitoring and identifying new bacterial species in hospital environments. species is considered an opportunistic human pathogen that can cause multiple types of invasive and severe infections in patients with underlying pathologies. Treatment of these pathogens has become a major challenge because many isolates are resistant to most antibiotics used in clinical practice. Furthermore, there are no consistent therapeutic results demonstrating the efficacy of antibacterial agents. Although considered a rare pathogen, recent studies have provided evidence of the emergence of in clinical settings. Hence, we identified a novel pathogenic bacterium, sp. nov., that presented a multidrug resistance (MDR) profile and carried diverse genes related to drug resistance, virulence, and mobile genetic elements. Such data can serve as a baseline for understanding the genomic diversity, adaptation, evolution, and pathogenicity of MDR
Topics: Infant, Newborn; Humans; RNA, Ribosomal, 16S; Brazil; Anti-Bacterial Agents; Colistin; DNA
PubMed: 37067439
DOI: 10.1128/spectrum.04415-22