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Journal of Conservative Dentistry and... Jan 2024Bioceramic sealers have improved sealing ability by forming an interfacial apatite layer that chemically bonds the sealer and radicular dentin thus decrease apical...
CONTEXT
Bioceramic sealers have improved sealing ability by forming an interfacial apatite layer that chemically bonds the sealer and radicular dentin thus decrease apical leakage.
AIM
This study aims to evaluate and compare the apical leakage of Cerafill RCS bioceramic sealer and gutta percha when used with three different obturating techniques.
MATERIALS AND METHODS
Thirty-four extracted single-rooted premolars were decoronated and prepared up to size F3. Then, the specimens were randomly divided into 3 experimental groups ( = 10) cold lateral obturation technique, warm vertical obturation technique, single-cone obturation technique, positive and negative control groups ( = 2), according to the obturation technique used along with a bioceramic sealer. To evaluate apical leakage, all specimens were mounted in a glucose leakage model and assessed at 7 and 14 days using an ultraviolet-visible spectrophotometer.
STATISTICAL ANALYSIS
The results were subjected to ANOVA/Kruskal-Wallis ANOVA; followed by post hoc analysis using Bonferroni correction.
RESULTS
Significant differences were found in the cumulative leakage of all the three experimental groups. Significantly higher leakage was found in groups obturated using single-cone obturation technique as compared to warm vertical compaction technique at both 7 and 14 days.
CONCLUSIONS
Warm vertical compaction showed a better sealing result than single-cone obturation techniques at all observation periods.
PubMed: 38389733
DOI: 10.4103/jcd.jcd_303_23 -
BMC Oral Health Feb 2024Sodium hypochlorite (NaOCl) is the most efficient root canal irrigant to date. The aim of this study was to compare the effect of NaOCl used at different temperatures...
BACKGROUND
Sodium hypochlorite (NaOCl) is the most efficient root canal irrigant to date. The aim of this study was to compare the effect of NaOCl used at different temperatures and concentrations on the compressive strength of root dentin.
MATERIALS AND METHODS
Seventy-two extracted human single-canaled straight roots of comparable size and length were selected and randomly divided into six groups (n = 12): Group (A) served as a control with unprepared canals. The other groups were instrumented with rotary ProTaper Universal files up to size F3. Group (B) was irrigated with 1% NaOCl at room temperature, Group (C) with 1% NaOCl heated to 70 °C, Group (D) with 5.25% NaOCl at room temperature, and Group (E) with 5.25% NaOCl heated to 70 °C. Saline was used in Group (F). The roots were sectioned into 2-mm-thick disks that underwent compression testing using a universal testing machine. Data were analyzed using one-way ANOVA and post hoc Tukey tests. The significance level was set at p ≤ 0.05.
RESULTS
A total of 255 disks were tested. The control group showed the highest compressive strength (p = 0.0112). However, this did not differ significantly from that of heated (p = 0.259) or unheated (p = 0.548) 1% NaOCl. There were no statistically significant differences between the groups of instrumented teeth.
CONCLUSION
Within the conditions of this study, irrigation with NaOCl at different concentrations and temperatures during root canal preparation did not affect the compressive strength of root dentin.
CLINICAL RELEVANCE
This study demonstrates that the use of NaOCl as a root canal irrigant is not associated with a clinically relevant decrease in root compressive strength, especially when compared to saline.
Topics: Humans; Dental Pulp Cavity; Dentin; Root Canal Irrigants; Root Canal Preparation; Sodium Hypochlorite; Temperature; Tooth
PubMed: 38350980
DOI: 10.1186/s12903-024-03954-y -
Clinical and Experimental Dental... Feb 2024This study assessed whether combining photobiomodulation therapy (PBMT) with casein phosphopeptide-amorphous calcium phosphate fluoride (CPP-ACPF) paste can effectively... (Review)
Review
The effect of photobiomodulation therapy associated with casein phosphopeptide-amorphous calcium phosphate fluoride paste on the treatment of posthome whitening tooth sensitivity and color change: A randomized clinical trial.
OBJECTIVE
This study assessed whether combining photobiomodulation therapy (PBMT) with casein phosphopeptide-amorphous calcium phosphate fluoride (CPP-ACPF) paste can effectively reduce post-home whitening tooth sensitivity (TS) without compromising shade change.
METHODS
Fifty participants were selected and assigned to one of four groups: (1) PLACEBO group-received a placebo paste and PBMT simulation; (2) PBMT group-received a placebo paste + PBMT; (3) CPP-ACPF group-received CPP-ACPF paste and PBMT simulation; (4) CPP-ACPF + PBMT group-received both CPP-ACPF paste and PBMT. The participants used whitening trays containing 22% carbamide peroxide for 2 h a day for 21 days. TS was measured daily using a visual analog scale, while shade change was assessed using a spectrophotometer: before bleaching treatment (T0), after the first (T1), second (T2), and third (T3) weeks of treatment, and 30 days (T4) after completing the whitening treatment.
RESULTS
Intragroup analysis revealed that the PLACEBO group had the highest increase in sensitivity during the whitening treatment. The CPP-ACPF and PBMT groups showed no significant difference tooth whitening (TW) between weeks regarding aesthetic change. The CPP-ACPF and PBMT group exhibited a significant reduction in TS between the first and third and between the second and third weeks TW, but not between the first and second. Conversely, the PLACEBO group showed a higher sensitivity than the other groups (p < .05). The CPP-ACPF and PBMT groups did not differ from each other. Furthermore, the CPP-ACPF and PBMT group showed a greater decrease in sensitivity than the PLACEBO group at T1, T2, and T3 (p < .01), and was significantly differed from CPP-ACPF and PBMT groups only at T2 and T3. All groups confirmed TW effectiveness. Student's and paired t-test did not reveal any significant difference between groups (p > .05).
CONCLUSION
Therefore, PBMT associated with CPP-ACPF paste can reduce TS without compromising the efficacy of TW.
Topics: Humans; Fluorides; Dentin Sensitivity; Caseins; Phosphopeptides; Low-Level Light Therapy; Randomized Controlled Trials as Topic; Calcium Phosphates
PubMed: 38345511
DOI: 10.1002/cre2.817 -
Journal of Dental Sciences Jan 2024Type 2 diabetes mellitus (T2DM), characterized by hyperglycemia, is a systematic disease affecting structure and healing ability in various tissues. This study aimed to...
BACKGROUND/PURPOSE
Type 2 diabetes mellitus (T2DM), characterized by hyperglycemia, is a systematic disease affecting structure and healing ability in various tissues. This study aimed to investigate whether T2DM could impair the dental pulp healing and cause underlying pathological changes in the dental pulp before an injury occurred.
MATERIALS AND METHODS
Goto-Kakizaki rats were used as T2DM model animals and performed with direct pulp capping procedures on the first maxillary molars. The molars at 1, 2, 4 weeks after operation and non-injured molars were examined using hematoxylin and eosin staining, immunohistochemical staining, immunofluorescence staining, and Masson's trichrome staining. The fresh dental pulp of maxillary incisors was collected for transmission electron microscopy and glucose content evaluation.
RESULTS
The T2DM rats showed deficient reparative dentin formation compared with the healthy rats. Before the occurrence of an injury, underlying pathological changes of major components in the pulp tissue were observed in T2DM rats, including vasculopathy; collagen abnormalities; decreased proliferation, decreased odontogenetic differentiation and damaged ultrastructure of dental pulp cells. High glucose content and advanced glycation end products accumulation were further found in the pulp tissue in T2DM rats.
CONCLUSION
T2DM can impede pulp healing process in rats, which is associated with underlying pathological changes in the non-injured pulp caused by the advanced glycation end products accumulation under high-glucose conditions.
PubMed: 38303798
DOI: 10.1016/j.jds.2023.03.021 -
Cureus Jan 2024Introduction Dental erosion from acidogenic diets, vigorous tooth brushing, excessive tooth whitening, gingival recession, periodontal debridement, or surgery may induce...
Introduction Dental erosion from acidogenic diets, vigorous tooth brushing, excessive tooth whitening, gingival recession, periodontal debridement, or surgery may induce dentine hypersensitivity (DH). It manifests as a phenomenon observed in structurally intact teeth devoid of pathological or defective conditions. Hence, this study aimed to evaluate DH risk reduction after using Fluoraphat Pro (Neumunster, Germany) and VivaSens (Ivoclar Vivadent, Schaan, Liechtenstein, Switzerland) in-office desensitizing agents. Method Twenty-two participants with at least two hypersensitive teeth were randomly selected. Participants were divided into two groups with 22 teeth each, and a desensitizing agent (Fluoraphat Pro and Vivasens) was applied accordingly. Participants were recalled the next day, and a decrease in sensitivity (if any) was evaluated by self-reporting using a 5-point Likert-type scale. Relative Risk (RR) was used to compare the likelihood of sensitivity between two groups and Cohen's d to measure the effect size. A value of p<0.05 was considered significant for all statistical purposes. Result The relative risk (RR) results indicated that Fluoraphat Pro has significantly lower RR than Vivasens for patients with dental sensitivity (RR = 0.350, 95% CI 0.187 - 0.654, z = 3.28, p = 0.001). Fluoraphat Pro was significantly more effective concerning lower sensitivity in teeth located in the upper right (dCohen = 3.217, p = 0.038) and lower right (dCohen = 3.193, p = 0.017) of the mouth than VivaSens®. Conclusion The two commercially available desensitizing agents Fluoraphat Pro and Vivasens tested in this study were effective in controlling DH. Fluoraphat Pro was more efficient in relieving the risk of dental hypersensitivity than VivaSens®. Further research is needed to evaluate the long-term effects of the desensitizing agents and compare DH reduction with other marketed desensitizing products.
PubMed: 38298297
DOI: 10.7759/cureus.51463 -
European Review For Medical and... Jan 2024This study aimed to assess the antimicrobial effectiveness of a new disinfection regimen Curcumin photosensitizer (CP), Synchronized Microbubble Photodynamic Activation...
Extrusion bond strength and disinfection of E. faecalis from canal dentin using synchronized microbubble photodynamic activation and photon-induced photoacoustic streaming.
OBJECTIVE
This study aimed to assess the antimicrobial effectiveness of a new disinfection regimen Curcumin photosensitizer (CP), Synchronized Microbubble Photodynamic Activation (SYMPA), Photon-induced photoacoustic streaming (PIPS), and its impact on the extrusion bond strength (EBS) of root filling material to canal dentin.
MATERIALS AND METHODS
Root canal treatments of sixty mandibular incisors were performed, and an overnight culture of E. faecalis was cultivated in the BHI medium. A volume of 1 mL was introduced into twenty root canals. All the samples were randomly allocated into 4 groups based on the irrigation used: -Group 1: 2.5% NaOCl+17% EDTA (Control), Group 2: CP+17% EDTA, Group 3: SYMPA+17% EDTA, and Group 4: PIPS+17% EDTA. The viable bacterial count was estimated, and 15 specimens from each group were obturated, followed by sectioning in 1-mm thick slices. The EBS was measured using a universal testing machine, and failure modes were analyzed using a stereomicroscope at 40x magnification. Means and standard deviations (SD) of the survival rate of E. faecalis and EBS of root filling to the dentin were analyzed using ANOVA Tukey multiple comparison t-tests (p=0.05).
RESULTS
Group 1 (2.5% NaOCl+17% EDTA) demonstrated the highest survival rate of E. faecalis. Group 3, in which SYMPA+17% EDTA was used to sterilize the canal, unveiled the lowest survival rate (1.55±0.11 CFU/mL). Similarly, the coronal section of Group 3 specimens (8.67±0.43MPa) demonstrated the highest EBS. However, an apical section of Group 1 samples (2.81±0.11MPa) displayed the lowest outcome of bond integrity. Intergroup comparison analysis revealed that Group 4 (PIPS+17% EDTA) and Group 2 (CP+17% EDTA) samples demonstrated comparable values of bond integrity and bacterial survival.
CONCLUSIONS
Canal disinfection using the synchronized microbubble photodynamic activation (SYMPA) technique proved to be a promising alternative technique in decreasing the count of E. faecalis and improving extrusion bond strength of gutta percha to canal dentin.
Topics: Root Canal Filling Materials; Dental Pulp Cavity; Edetic Acid; Disinfection; Microbubbles; Root Canal Preparation; Root Canal Irrigants; Photosensitizing Agents; Dentin; Materials Testing; Photochemotherapy
PubMed: 38235856
DOI: 10.26355/eurrev_202401_34888 -
Nanoscale Advances Jan 2024Calcium carbonate (CaCO), possessing excellent biocompatibility, bioactivity, osteoconductivity and superior biodegradability, may serve as an alternative to...
Calcium carbonate (CaCO), possessing excellent biocompatibility, bioactivity, osteoconductivity and superior biodegradability, may serve as an alternative to hydroxyapatite (HAp), the natural inorganic component of bone and dentin. Intrafibrillar mineralization of collagen with CaCO was achieved through the polymer-induced liquid precursor (PILP) process for at least 2 days. This study aims to propose a novel pathway for rapid intrafibrillar mineralization with CaCO by sequential application of the carbonate-bicarbonate buffer and polyaspartic acid (pAsp)-Ca suspension. Fourier transform infrared (FTIR) spectroscopy, zeta potential measurements, atomic force microscopy/Kelvin probe force microscopy (AFM/KPFM), and three-dimensional stochastic optical reconstruction microscopy (3D STORM) demonstrated that the carbonate-bicarbonate buffer significantly decreased the surface potential of collagen and CO/HCO ions could attach to collagen fibrils hydrogen bonds. The electropositive pAsp-Ca complexes and free Ca ions are attracted to and interact with CO/HCO ions through electrostatic attractions to form amorphous calcium carbonate that crystallizes gradually. Moreover, like CaCO, strontium carbonate (SrCO) can deposit inside the collagen fibrils through this pathway. The CaCO-mineralized collagen gels exhibited better biocompatibility and cell proliferation ability than SrCO. This study provides a feasible strategy for rapid collagen mineralization with CaCO and SrCO, as well as elucidating the tissue engineering of CaCO-based biomineralized materials.
PubMed: 38235102
DOI: 10.1039/d3na00705g -
PloS One 2024This study aimed to evaluate the dislodgement resistance and structural changes of different mineral trioxide aggregate cements (MTA) like Pro-Root MTA, Ortho MTA, and...
This study aimed to evaluate the dislodgement resistance and structural changes of different mineral trioxide aggregate cements (MTA) like Pro-Root MTA, Ortho MTA, and Retro MTA after exposure to sodium hypochlorite (NaOCl), NaOCl-Ethylenediaminetetraacetic acid (EDTA), 1-hydroxyethylidene-1, 1-bisphosphonate (Dual Rinse HEDP), and NaOCl-Maleic acid (MA). The root canal spaces of 150 dentine slices were obturated using tricalcium silicate cements and divided into 3 groups (n = 50): Group1: ProRoot MTA, Group2: Retro MTA, and Group3: Ortho MTA. The samples in each group were further subdivided into four experimental (n = 10) and one control groups (n = 10): 2.5% NaOCl-17% EDTA, Dual Rinse HEDP, 2.5% NaOCl-7% Maleic acid, 2.5% NaOCl, distilled water (control). The dislodgement resistance and structural changes of cements were measured. Use of DR HEDP resulted in higher dislodgement resistance compared to17% EDTA and 7% MA in the samples obturated with Ortho MTA and Pro-Root MTA (p<0.001). In Retro MTA group, samples treated with DR HEDP and 17% EDTA had higher dislodgment resistance compared to 7% MA (p<0.001). On microstructural and elemental analysis of all the three MTA cements, samples treated with 17% EDTA and 7% MA were more amorphous and granular when compared to DR HEDP, which was pettle shaped. Calcium level was decreased more in samples treated with 17% EDTA and 7% MA when compared to DR HEDP.
Topics: Chelating Agents; Edetic Acid; Etidronic Acid; Calcium Compounds; Silicates; Drug Combinations; Oxides; Maleates
PubMed: 38232127
DOI: 10.1371/journal.pone.0296647 -
Journal of Prosthodontic Research Jan 2024This narrative review aimed to survey the clinical outcomes of laminate veneers (LVs), including their survival and success rates.
PURPOSE
This narrative review aimed to survey the clinical outcomes of laminate veneers (LVs), including their survival and success rates.
STUDY SELECTION
An electronic search of MEDLINE/PubMed, Web of Science, Cochrane Library, and Google Scholar from 2000 to April 2023 was conducted using the keywords "laminate" OR "veneer" OR "porcelain" OR "feldspathic" OR "lithium disilicate" OR "composite resin" OR "zirconia" OR "survival" OR "success." Case reports, case series, reviews, abstracts, in vitro studies, and observational studies were excluded. Five researchers independently evaluated the titles and abstracts of all identified studies.
RESULTS
A total of 55 studies were identified. None of the studies met the criteria for zirconia LVs. According to the studies in this review, LVs fabricated with feldspathic porcelain, leucite-reinforced glass ceramics (LRG), and lithium disilicate ceramics (LDS) exhibited satisfactory survival and success rates. Furthermore, hydrofluoric acid etching followed by silane priming of the surface of ceramic LVs is necessary for improved clinical outcomes. The extent of dentin exposure significantly decreases the success rate of ceramic LVs. An appropriate adhesive luting process is required to achieve the long-term success of ceramic LVs. Dentin exposure should be minimized or sealed during tooth preparation to achieve a reliable and durable bond between LVs and abutment teeth.
CONCLUSIONS
Based on this narrative review of the literature, the use of silica-based ceramic feldspathic porcelain, LRG, and LDS is recommended for LVs.
PubMed: 38220160
DOI: 10.2186/jpr.JPR_D_23_00151 -
Journal of Translational Medicine Jan 2024Epigenetic factors influence the odontogenic differentiation of dental pulp stem cells and play indispensable roles during tooth development. Some microRNAs can...
BACKGROUND
Epigenetic factors influence the odontogenic differentiation of dental pulp stem cells and play indispensable roles during tooth development. Some microRNAs can epigenetically regulate other epigenetic factors like DNA methyltransferases and histone modification enzymes, functioning as epigenetic-microRNAs. In our previous study, microarray analysis suggested microRNA-93-5p (miR-93-5p) was differentially expressed during the bell stage in human tooth germ. Prediction tools indicated that miR-93-5p may target lysine-specific demethylase 6B (KDM6B). Therefore, we explored the role of miR-93-5p as an epi-miRNA in tooth development and further investigated the underlying mechanisms of miR-93-5p in regulating odontogenic differentiation and dentin formation.
METHODS
The expression pattern of miR-93-5p and KDM6B of dental pulp stem cells (DPSCs) was examined during tooth development and odontogenic differentiation. Dual luciferase reporter and ChIP-qPCR assay were used to validate the target and downstream regulatory genes of miR-93-5p in human DPSCs (hDPSCs). Histological analyses and qPCR assays were conducted for investigating the effects of miR-93-5p mimic and inhibitor on odontogenic differentiation of hDPSCs. A pulpotomy rat model was further established, microCT and histological analyses were performed to explore the effects of KDM6B-overexpression and miR-93-5p inhibition on the formation of tertiary dentin.
RESULTS
The expression level of miR-93-5p decreased as odontoblast differentiated, in parallel with elevated expression of histone demethylase KDM6B. In hDPSCs, miR-93-5p overexpression inhibited the odontogenic differentiation and vice versa. MiR-93-5p targeted 3' untranslated region (UTR) of KDM6B, thereby inhibiting its protein translation. Furthermore, KDM6B bound the promoter region of BMP2 to demethylate H3K27me3 marks and thus upregulated BMP2 transcription. In the rat pulpotomy model, KDM6B-overexpression or miR-93-5p inhibition suppressed H3K27me3 level in DPSCs and consequently promoted the formation of tertiary dentin.
CONCLUSIONS
MiR-93-5p targets epigenetic regulator KDM6B and regulates H3K27me3 marks on BMP2 promoters, thus modulating the odontogenic differentiation of DPSCs and dentin formation.
Topics: Humans; Rats; Animals; Histones; Stem Cells; Cell Differentiation; MicroRNAs; Dentin; Cells, Cultured; Jumonji Domain-Containing Histone Demethylases
PubMed: 38218880
DOI: 10.1186/s12967-024-04862-z