-
ACS Omega Jun 2023Immobilization of enzymes has been widely reported due to their reusability, thermal stability, better storage abilities, and so on. However, there are still problems...
Immobilization of enzymes has been widely reported due to their reusability, thermal stability, better storage abilities, and so on. However, there are still problems that immobilized enzymes do not have free movements to react to substrates during enzyme reactions and their enzyme activity becomes weak. Moreover, when only the porosity of support materials is focused, some problems such as enzyme distortion can negatively affect the enzyme activity. Being a solution to these problems, a new function "floatability" of enzyme devices has been discussed. A "floatable" micron-sized enzyme device was fabricated to enhance the free movements of immobilized enzymes. Diatom frustules, natural nanoporous biosilica, were used to attach papain enzyme molecules. The floatability of the frustules, evaluated by macroscopic and microscopic methods, was significantly better than that of four other SiO materials, such as diatomaceous earth (DE), which have been widely used to fabricate micron-sized enzyme devices. The frustules were fully suspended at 30 °C for 1 h without stirring, although they settled at room temperature. When enzyme assays were performed at room temperature, 37, and 60 °C with or without external stirring, the proposed frustule device showed the highest enzyme activity under all conditions among papain devices similarly prepared using other SiO materials. It was confirmed by the free papain experiments that the frustule device was active enough for enzyme reactions. Our data indicated that the high floatability of the reusable frustule device, and its large surface area, is effective in maximizing enzyme activity due to the high probability to react to substrates.
PubMed: 37332799
DOI: 10.1021/acsomega.3c02104 -
Marine Drugs Apr 2023Colorectal cancer is among the most prevalent and lethal cancers globally. To address this emergency, countries have developed diffuse screening programs and innovative... (Review)
Review
Colorectal cancer is among the most prevalent and lethal cancers globally. To address this emergency, countries have developed diffuse screening programs and innovative surgical techniques with a consequent decrease in mortality rates in non-metastatic patients. However, five years after diagnosis, metastatic CRC is still characterized by less than 20% survival. Most patients with metastatic CRC cannot be surgically treated. For them, the only option is treatment with conventional chemotherapies, which cause harmful side effects in normal tissues. In this context, nanomedicine can help traditional medicine overcome its limits. Diatomite nanoparticles (DNPs) are innovative nano-based drug delivery systems derived from the powder of diatom shells. Diatomite is a porous biosilica largely found in many areas of the world and approved by the Food and Drug Administration (FDA) for pharmaceutical and animal feed formulations. Diatomite nanoparticles with a size between 300 and 400 nm were shown to be biocompatible nanocarriers capable of delivering chemotherapeutic agents against specific targets while reducing off-target effects. This review discusses the treatment of colorectal cancer with conventional methods, highlighting the drawbacks of standard medicine and exploring innovative options based on the use of diatomite-based drug delivery systems. Three targeted treatments are considered: anti-angiogenetic drugs, antimetastatic drugs, and immune checkpoint inhibitors.
Topics: Animals; Nanomedicine; Diatoms; Diatomaceous Earth; Drug Delivery Systems; Nanoparticles; Colorectal Neoplasms; Antineoplastic Agents
PubMed: 37233460
DOI: 10.3390/md21050266 -
ACS Omega May 2023Optofluidic sensors have accelerated the growth of smart sensor platforms with improved sensitivity, reliability, and innovation. In this article, we report the...
Optofluidic sensors have accelerated the growth of smart sensor platforms with improved sensitivity, reliability, and innovation. In this article, we report the integration of a surface-enhanced Raman scattering (SERS) material consisting of silver nanoparticle-decorated diatomaceous earth (AgNPs-DE) with a flow-through microfluidic device, building up a hierarchical structured micro-total analysis system (μ-TAS) capable of achieving part-per-quadrillion (ppq)-level sensitivity. By the synergic integration of millimeter-scale microfluidic devices and porous laboratory filter paper with a micrometer-sized crosslinked cellulosic network that carries SERS-active AgNPs-DE, which possesses submicron to nanometer regimes of photonic crystals and plasmonic nanostructures, we achieved enhanced mass-transfer efficiency and unprecedented detection sensitivity. In our experiment, fentanyl as the testing analyte at different concentrations was measured using a portable Raman spectrometer. The limit of detection (LOD) was estimated to be 10 ppq from a small detection volume of 10 mL with an ultrafast time of sensing (TOS) of 3 min. To attain comparable signals, the traditional soaking method took more than 90 min to detect 10 part-per-trillion fentanyl from a 10 mL sample. Compared with existing SERS sensing results of fentanyl, the limit-defying μ-TAS reduced the LOD-TOS product by almost 4 orders of magnitude, which represents a new stage of ultrafast sensing of extremely low concentration analytes.
PubMed: 37214736
DOI: 10.1021/acsomega.3c01519 -
Science & Justice : Journal of the... May 2023Environmental trace evidence offers useful circumstantial intelligence to link persons and scenes of forensic interest. An increasing empirical research base is...
Environmental trace evidence offers useful circumstantial intelligence to link persons and scenes of forensic interest. An increasing empirical research base is dedicated towards understanding the transfer and persistence dynamics of environmental indicators including pollen, soils, and diatoms, within a diverse range of experimental frameworks. This paper presents two discrete studies exploring transfer and persistence of soils and sediments on footwear and diatomaceous earth adhered to clothing in forensically pertinent scenarios. Variables including sediment type, foot position, clothing type, and body positioning were also explored throughout. Both experiments incorporated a field-based methodology during the sampling effort. Photographs were collected of an initial transfer sample and of a retained assemblage following hours, days, and up to one-week of wear, facilitating macroscopic assessment of trace evidence dynamics. All images were processed using accessible, open-source software before spatial analysis of evidence distribution within and temporal assessment (% retention) upon each evidential surface. The results highlighted consistent loss of transferred sediment from footwear with significantly greater retention of loamy clay soil than dune sand which was absent beyond 24 h of wear. Loss was not influenced by wearer gait but was more rapid from those areas of the shoe sole in direct contact with the ground. Diatomaceous earth was retrieved from all three clothing types tested after one week - significant losses of material occurred before 48 h with a consistent assemblage identified beyond this. Denim was significantly more effective than acrylic and fleece for diatomaceous earth retention and significantly more material was lost from clothing worn on the lower body. These findings highlight the value of using visual environmental markers and a macroscopic analytical approach during the investigation of environmental trace dynamics. The methodology offers a novel, non-destructive assessment of soil and diatom transfer and persistence, complementing more extensive laboratory-based examinations to ensure the development of a well-rounded research base within the forensic sciences.
Topics: Humans; Clothing; Diatomaceous Earth; Forensic Medicine; Shoes; Forensic Sciences; Soil
PubMed: 37169463
DOI: 10.1016/j.scijus.2023.03.004 -
Materials (Basel, Switzerland) Mar 2023The aim of this work is to investigate the possibility of reusing ashes obtained by the calcination of industrial sludge from the oil refining industry (ORSA) as a...
The aim of this work is to investigate the possibility of reusing ashes obtained by the calcination of industrial sludge from the oil refining industry (ORSA) as a secondary raw material in the manufacture of alkaline activated cements or geopolymers. The incorporation behavior of 5-20 wt.% of residue in binary mixtures with rice husk ash (RHA) or chamotte (CHM) was evaluated. The cements were activated with a sustainable alternative activating solution obtained from NaOH (10 M) and diatomaceous earth. The specimens were cured at room temperature. Physical and mechanical properties were determined, and the reaction products were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy-energy dispersive X-ray spectroscopy (SEM-EDX). The results indicate that the addition of ORSA (5-20 wt.%) to RHA and CHM improves the mechanical strength of alkaline activated cements with maximum compressive strengths of 30.6 MPa and 15.7 MPa, respectively, after 28 days of curing, with the incorporation of 20 wt.% waste. In these mixtures, the sludge acts as a source of aluminum, promoting the formation of a higher amount of geopolymer gel N-A-S-H in materials using RHA as a precursor and also (N)-(C)-A-S-H gel in cements using CHM.
PubMed: 37049095
DOI: 10.3390/ma16072801 -
Journal of Biotechnology Apr 2023Adeno-associated virus (AAV) based vectors have recently been gaining importance as DNA delivery systems. Efficient downstream processing of AAV remains a major...
Adeno-associated virus (AAV) based vectors have recently been gaining importance as DNA delivery systems. Efficient downstream processing of AAV remains a major challenge as serotypes differ in physicochemical properties, making it difficult to design uniform purification processes. Clarification of AAV is an especially critical step. Harvesting of AAV, like other viruses, often requires cell lysis, resulting in a difficult-to-filter cell lysate. In this study, we evaluated the applicability of diatomaceous earth (DE) as a filter aid for clarification of AAV crude cell lysates. DE filtration proved to be a viable clarification method for AAV2, AAV5 and AAV8. Based on a design of experiment approach, the DE concentration was identified as the main factor influencing AAV particle loss. The loss of AAV during DE filtration was limited to < 2% by maintaining the DE quantity below 0.181 mg DE/10 AAV. Use of DE reduced manual handling time 3-fold and increased the filter capacity 3.5-fold compared to filtration combined with a prior centrifugation step. Moreover, we showed that the DE type had only a minor influence on the filtration performance. This study demonstrated that filtration with DE as a filter aid is an efficient clarification method for different AAV serotypes.
Topics: Dependovirus; Genetic Vectors; DNA; Serogroup
PubMed: 36997072
DOI: 10.1016/j.jbiotec.2023.03.010 -
Polymers Mar 2023Amorphic diatomaceous earth is derived from natural sources, and polyamide 11 (PA11) is produced from materials of natural origin. Both of these materials show a low...
Amorphic diatomaceous earth is derived from natural sources, and polyamide 11 (PA11) is produced from materials of natural origin. Both of these materials show a low harmfulness to the environment and a reduced carbon footprint. This is why the combination of these two constituents is beneficial not only to improve the physicochemical and mechanical properties of polyamide 11 but also to produce a biocomposite. For the purpose of this paper, the test biocomposite was produced by combining polyamide 11, as well as basic and pre-fractionated diatomaceous earth, which had been subjected to silanization. The produced composites were used to carry out rheological (melt flow rate-MFR), mechanical (tensile strength, bending strength, impact strength), crystallographic (X-ray Diffraction-XRD), thermal and thermo-mechanical (differential scanning calorimetry-DSC, dynamic mechanical thermal analysis-DMTA) analyses, as well as a study of hydrophobic-hydrophilic properties of the material surface (wetting angle) and imaging of the surface of the composites and the fractured specimens. The tests showed that the additive 3-aminopropyltriethoxysilane (APTES) acted as an agent that improved the elasticity of composites and the melt flow rate. In addition, the produced composites showed a hydrophilic surface profile compared to pure polylactide and polyamide 11.
PubMed: 36987343
DOI: 10.3390/polym15061563 -
Forensic Science International May 2023Diatoms (Bacillariophyta) are unicellular photosynthetic organisms commonly occurring in aquatic habitats on Earth. Their autecology makes them almost perfect indicators...
Diatoms (Bacillariophyta) are unicellular photosynthetic organisms commonly occurring in aquatic habitats on Earth. Their autecology makes them almost perfect indicators of environmental conditions and so have high potential for use in forensics. Both eurytopic and stenotopic species are important in site identification: eurytopic forms due to their abundance, and stenotopic forms due to their narrow range of tolerance to environmental conditions. Their presence can hence provide a clear insight into an ecosystem and its microhabitats. The diatoms are useful as significant indicator in diagnosing of drowning. However, to definitively link a corpse with the place where it was found and to indicate whether this was also the crime scene, it is essential that any comparative diatomaceous analyses are performed correctly. The following study presents selected cases in which a comparative diatomaceous analysis was performed. In all cases, the biological samples secured during the autopsy were compared with environmental samples collected from the site of the cadaver disclosure. Our findings show both the possibilities and limitations of using this method in forensics. These forensic investigations need close collaboration between coroners and diatomology experts. It is crucially important to reveal whether the place of corpse finding is the same as the place of drowning.
Topics: Humans; Cadaver; Diatoms; Drowning; Ecosystem; Lung; Forensic Sciences; Environment
PubMed: 36958086
DOI: 10.1016/j.forsciint.2023.111644 -
Materials (Basel, Switzerland) Feb 2023The clarity of the beer is essential to its marketability and good consumer approval. Moreover, the beer filtration aims to remove the unwanted constituents that cause...
The clarity of the beer is essential to its marketability and good consumer approval. Moreover, the beer filtration aims to remove the unwanted constituents that cause beer haze formation. Natural zeolite, an inexpensive and widespread material, was tested as a substitute filter media for diatomaceous earth in removing the haze constituents in beer. The zeolitic tuff samples were collected from two quarries in Northern Romania: Chilioara, in which the zeolitic tuff has a clinoptilolite content of about 65%, and the Valea Pomilor quarry, containing zeolitic tuff with a clinoptilolite content of about 40%. Two-grain sizes, <40 and <100 µm, from each quarry were prepared and thermally treated at 450 °C in order to improve their adsorption properties and remove organic compounds and for physico-chemical characterization. The prepared zeolites were used for beer filtration in different mixtures with commercial filter aids (DIF BO and CBL3) in laboratory-scale experiments, and the filtered beer was characterized in terms of pH, turbidity, color, taste, flavor, and concentrations of the major and trace elements. The results showed that the taste, flavor, and pH of the filtered beer were generally not affected by filtration, while turbidity and color decreased with an increase in the zeolite content used in the filtration. The concentrations of Na and Mg in the beer were not significantly altered by filtration; Ca and K slowly increased, while Cd and Co were below the limits of quantification. Our results show that natural zeolites are promising aids for beer filtration and can be readily substituted for diatomaceous earth without significant changes in brewery industry process equipment and protocols for preparation.
PubMed: 36903029
DOI: 10.3390/ma16051914 -
Biosensors Feb 2023(MTB) is a communicable disease and still remains a threat to common health. Thus, early diagnosis and treatment are required to prevent the spread of infection....
(MTB) is a communicable disease and still remains a threat to common health. Thus, early diagnosis and treatment are required to prevent the spread of infection. Despite the recent advances in molecular diagnostic systems, the commonly used MTB diagnostic tools are laboratory-based assays, such as mycobacterial culture, MTB PCR, and Xpert MTB/RIF. To address this limitation, point-of-care testing (POCT)-based molecular diagnostic technologies capable of sensitive and accurate detection even in environments with limited sources are needed. In this study, we propose simple tuberculosis (TB) molecular diagnostic assay by combining sample preparation and DNA-detection steps. The sample preparation is performed using a syringe filter with amine-functionalized diatomaceous earth and homobifunctional imidoester. Subsequently, the target DNA is detected by quantitative PCR (polymerase chain reaction). The results can be obtained within 2 h from samples with large volumes, without any additional instruments. The limit of detection of this system is 10 times higher than those of conventional PCR assays. We validated the clinical utility of the proposed method in 88 sputum samples obtained from four hospitals in the Republic of Korea. Overall, the sensitivity of this system was superior to those of other assays. Therefore, the proposed system can be useful for MTB diagnosis in limited-resource settings.
Topics: Humans; Mycobacterium tuberculosis; Tuberculosis, Pulmonary; Pathology, Molecular; Sensitivity and Specificity; Sputum; Molecular Diagnostic Techniques
PubMed: 36832025
DOI: 10.3390/bios13020259