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Clinical and Experimental Immunology Jun 1980The Fischer rat develops an acquired resistance against circulating microfilariae. Macrophages from the peritoneal washings of normal rats preincubated at 37 degrees C...
The Fischer rat develops an acquired resistance against circulating microfilariae. Macrophages from the peritoneal washings of normal rats preincubated at 37 degrees C with the sera obtained from rats immune to circulating microfilariae adhered to and kill the microfilaria of Dipetalonema viteae in vitro within 16 to 24 hr. No significant adherence and cytotoxicity was mediated by sera collected from animals with microfilaraemia or from normal rats. Adherence of macrophages to microfilaria was associated with damage to the surface of the larva as revealed by ultrastructural studies. Neither adherence nor cytotoxicity was induced by preincubation of microfilariae, instead of macrophages with immune serum. The serum factor which mediated adherence and cytotoxicity was heat-labile, but was not a complement component. Immune absorption experiments showed that the relevant serum factor resided in the IgE class of antibody. The immune adherence to D. viteae by macrophages is stage-specific because adherence to infective larvae was not observed whether rate macrophages were preincubated in sera obtained from rats immune to microfilariae or in sera collected from animals after exposure to infective larvae.
Topics: Animals; Antibody-Dependent Cell Cytotoxicity; Ascitic Fluid; Cell Adhesion; Dipetalonema; Dipetalonema Infections; Immune Sera; Immunity, Active; Immunoglobulin E; Macrophages; Male; Microfilariae; Rats; Rats, Inbred Strains
PubMed: 7191359
DOI: No ID Found -
Clinical and Experimental Immunology Nov 1979Serum IgE levels were measured by radioimmunoassay in rats infected with various doses of L3 infective stage larvae of Dipetalonema viteae. A high stimulation in total...
Serum IgE levels were measured by radioimmunoassay in rats infected with various doses of L3 infective stage larvae of Dipetalonema viteae. A high stimulation in total serum IgE levels was found with minute doses as well as with large doses of parasite, and IgE levels remained elevated for several months. No further increase in IgE levels was induced by a secondary infection.
Topics: Animals; Dipetalonema; Dipetalonema Infections; Dose-Response Relationship, Immunologic; Immunoglobulin E; Larva; Male; Rats; Rats, Inbred WF; Time Factors
PubMed: 575082
DOI: No ID Found -
Bulletin of the World Health... 1979In coastal Tanzania, an area where the microfilariae (mf) of Wuchereria bancrofti exhibit nocturnal periodicity, the administration of 2 mg diethylcarbamazine (DEC) per...
In coastal Tanzania, an area where the microfilariae (mf) of Wuchereria bancrofti exhibit nocturnal periodicity, the administration of 2 mg diethylcarbamazine (DEC) per kg body weight in the daytime provoked mf to enter the peripheral blood. In persons on normal daily activities the daytime DEC provocative method proved to be as sensitive in detecting microfilaraemia as was the examination of night blood. Its use in routine surveys is therefore justified. Although mf densities by day and night were highly correlated (r = 0.83) they tended to be lower after provocative daytime DEC than in the corresponding night blood, except in very light infections. This method was also useful in assessing the parasitological response to mass chemotherapy with DEC, but, in comparison with the results of the night blood examinations, the sensitivity and magnitude of the counts in persons remaining positive progressively decreased as the period of DEC administration increased. A correction factor has to be calculated to take account of this, and/or additional night blood samples must be taken.The dose of 2 mg of DEC per kg body weight used was readily acceptable to the people in coastal East Africa, whose cooperation is difficult to obtain for night blood surveys. Apart from W. bancrofti, the only human filarial infection occasionally encountered in this area was Dipetalonema perstans. Because of the risk of a severe Mazzotti reaction the test is contraindicated in onchocerciasis endemic regions. Severe reactions may also occur in subjects with loaiasis.
Topics: Circadian Rhythm; Diethylcarbamazine; Filariasis; Humans; Microfilariae; Tanzania; Wuchereria bancrofti
PubMed: 396052
DOI: No ID Found -
Bulletin of the World Health... 1976
Review
Topics: Aedes; Animals; Anopheles; Brugia; Dipetalonema; Diptera; Feeding Behavior; Filarioidea; Insect Vectors; Microfilariae; Onchocerca; Stomach; Wuchereria
PubMed: 799952
DOI: No ID Found -
Journal of Clinical Pathology Oct 1970Five laboratory methods used for the recovery of microfilariae from the blood were compared for efficiency of recovery and time involved. The methods used were thin... (Comparative Study)
Comparative Study
Five laboratory methods used for the recovery of microfilariae from the blood were compared for efficiency of recovery and time involved. The methods used were thin blood films, thick blood films, wet preparations, the Polyvidone technique, and the microhaematocrit technique. The last proved superior in both efficiency and saving time.
Topics: Dipetalonema; Filariasis; Filarioidea; Hematocrit; Humans; Methods; Povidone; Time Factors; Wuchereria
PubMed: 5529998
DOI: 10.1136/jcp.23.7.632 -
The Canadian Veterinary Journal = La... Aug 1969
Topics: Animals; Caniformia; Female; Filariasis; Heart Diseases; Lung Diseases, Parasitic; Pseudomonas Infections
PubMed: 5388277
DOI: No ID Found -
British Medical Journal Jan 1969
Topics: Arthritis; Dipetalonema; Humans; Nematode Infections
PubMed: 5812535
DOI: No ID Found -
Bulletin of the World Health... 1969
[Evaluation of 2 immunological tests (skin test and complement fixation test) for the detection of filariasis in populations of Upper Volta where Wuchereria bancrofti, Onchocercă volvulus and Dipetalonema perstans occur together].
Topics: Antigens; Burkina Faso; Complement Fixation Tests; Dipetalonema; Filariasis; Humans; Onchocerca; Skin Tests; World Health Organization; Wuchereria
PubMed: 5307597
DOI: No ID Found