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Journal of Athletic Training Aug 2022For this case series, 4 student-athletes (age range = 20-22 years) participating in National Collegiate Athletic Association Division I ice hockey served as cases. They...
CONTEXT
For this case series, 4 student-athletes (age range = 20-22 years) participating in National Collegiate Athletic Association Division I ice hockey served as cases. They were free of injury and participated in all team activities without restrictions.
TREATMENT
A dry needling (DN) lower extremity recovery protocol was completed for all athletes during a single session. To administer the DN recovery treatment, we placed static needles in specific bilateral locations that consisted of 5 points on both the anterior and posterior aspects of the lower extremity and lumbopelvic complex. The Acute Recovery Stress Scale was used to evaluate the effect of the DN recovery treatment on each athlete's perception of recovery at 24 and 48 hours post-DN treatment.
RESULTS
Overall, total and average scores of Acute Recovery Stress Scale for all cases were closer to baseline at 48 hours post-DN than at the other time points.
CONCLUSIONS
Recovery techniques historically have been used postactivity because even normal training loads, which are considered positive, produce stress and fatigue in athletes and can lead to injury. Results from this case series suggest that ice hockey athletes who are experiencing postexercise stress, such as soreness and fatigue, may benefit from a lower extremity DN recovery treatment protocol.
Topics: Humans; Young Adult; Athletes; Athletic Injuries; Dry Needling; Fatigue; Hockey; Universities
PubMed: 36356615
DOI: 10.4085/1062-6050-0096.21 -
Current Microbiology Nov 2022Shipment of COVID-19 specimens within the country or overseas at long distances requires cold chain facility using dry ice and triple packing to prevent the risk of...
Shipment of COVID-19 specimens within the country or overseas at long distances requires cold chain facility using dry ice and triple packing to prevent the risk of COVID-19 infection to the personnel involved in sample transport. The present study aimed to utilize FTA card technology as an alternate means of sample transport and storage across the country. Twenty-one SARS-CoV-2 lab confirmed samples with different Ct value (High, medium & low) were used to detect viral load in samples loaded on FTA card and further compared with VTM samples. The SARS-CoV-2 RNA was detected by rRT-PCR after storing for 14 days at 4 °C and 37 °C. The present study evaluated the utility of FTA cards for preserving the SARS CoV-2 RNA for 14-day period. A significant difference (P < 0.05) was observed in the cycle threshold (ΔCt 4-5) values obtained from FTA and VTM viral samples but it did not affect the positivity. The SARS-CoV-2 RNA could be recovered efficiently from FTA sample stored at 4 °C and 37 °C for 14 days. Thus, FTA cards could be an alternate option for transporting the samples at ambient temperature for a long time.
Topics: Humans; Specimen Handling; RNA, Viral; SARS-CoV-2; COVID-19; Refrigeration
PubMed: 36352332
DOI: 10.1007/s00284-022-03079-x -
Cell and Tissue Banking Jun 2023Availability of molecularly intact biospecimens is essential in genetic diagnostics to obtain credible results. Integrity of nucleic acids (particularly RNA) may be...
Availability of molecularly intact biospecimens is essential in genetic diagnostics to obtain credible results. Integrity of nucleic acids (particularly RNA) may be compromised at various steps of tissue handling, and affected by factors such as time to freeze, freezing technique and storing temperature. At the same time, freezing and storing of the biological material should be feasible and safe for the operator. Here, we compared quality of DNA and RNA from biospecimens derived from different organs (breast, colon, adrenal glands, testes, rectum and uterus) frozen either using dry ice-cooled isopentane or with FlashFREEZE unit, in order to verify if the latter is suitable for routine use in biobanking. Implementing FlashFREEZE device would enable us to limit the use of isopentane, which is potentially toxic and environmentally harmful, whilst facilitate standardization of sample freezing time. We considered factors such RNA and DNA yield and purity. Furthermore, RNA integrity and RNA/DNA performance in routine analyses, such as qPCR, next generation sequencing or microarray, were also assessed. Our results indicate that freezing of tissue samples either with FlashFREEZE unit or isopentane ensures biological material with comparable expression profiles and DNA mutation status, indicating that RNA and DNA of similar quality can be extracted from both. Therefore, our findings support the use of the FlashFREEZE device in routine use for biobanking purposes.
Topics: Humans; Biological Specimen Banks; Cryopreservation; Biopsy; Neoplasms; RNA; DNA
PubMed: 36309911
DOI: 10.1007/s10561-022-10045-1 -
Proceedings of the National Academy of... Oct 2022The Earth's climate has been warming rapidly since the beginning of the industrial era, forcing terrestrial organisms to adapt. Migration constitutes one of the most...
The Earth's climate has been warming rapidly since the beginning of the industrial era, forcing terrestrial organisms to adapt. Migration constitutes one of the most effective processes for surviving and thriving, although the speed at which tree species migrate as a function of climate change is unknown. One way to predict latitudinal movement of trees under the climate of the twenty-first century is to examine past migration since the Last Glacial Maximum. In this study, radiocarbon-dated macrofossils were used to calculate the velocity of past migration of jack pine () and black spruce (), two important fire-adapted conifers of the North American boreal forest. Jack pine migrated at a mean rate of 19 km per century (km) from unglaciated sites in the central and southeastern United States to the northern limit of the species in subarctic Canada. However, the velocity increased between unglaciated and early deglaciated sites in southern Quebec and slowed from early to mid-Holocene in central and eastern Quebec. Migration was at its lowest speed in late-Holocene times, when it stopped about 3,000 y ago. Compared with jack pine, black spruce migrated at a faster mean rate of 25 km from the ice border at the last interstadial (Bølling/Allerød) to the species tree limit. The modern range of both species was nearly occupied about 6,000 y ago. The factors modulating the changing velocity of jack pine migration were closely associated with the warm-dry climate of the late Pleistocene-Holocene transition and the more humid climate of the mid- and late-Holocene.
Topics: Canada; Fires; Ice; Picea; Pinus
PubMed: 36252032
DOI: 10.1073/pnas.2210496119 -
Frontiers in Molecular Biosciences 2022The freezing process of tissue samples is crucial for the preservation of morphological and molecular features. Several biobanking guidelines describe freezing...
The freezing process of tissue samples is crucial for the preservation of morphological and molecular features. Several biobanking guidelines describe freezing techniques for optimal outcomes. As the Vetbiobank standard freezing protocol does not comply with those recommendations in detail, a process validation was performed to demonstrate that samples are suitable for downstream applications. Here we give a formal example of a process validation in the biobanking setting, as required by the biobanking guideline ISO 20387 (2018). Three different freezing protocols, freezing in liquid nitrogen, freezing isopentane precooled on dry ice and freezing liquid nitrogen vapor, were assessed based on morphological integrity of mouse liver and muscle tissue samples. Samples were either frozen in cryotubes (without Optimal Cutting Temperature compound, OCT) or in cryomolds (with OCT). The protocol providing the best results was validated for reproducibility and robustness in terms of defined acceptance criteria for morphological evaluability, A260/A280 ratio, and RNA integrity number values (RIN). In addition, performance tests were run by gene expression analyzes of selected, tissue specific biomarkers to confirm that processed samples are fit for purpose. From the three applied freezing protocols, freezing in liquid nitrogen generated best results. Reproducibility acceptance criteria were met for both, morphological integrity and RNA quality. The freezing method was robust for the tested tissue types and the application of OCT, with exception of liver tissue, where it led to a significant decrease of the RIN value. Gene expression analyzes showed good comparability of results regardless of the applied freezing method. Freezing of tissue samples in liquid nitrogen provides samples of adequate quality for subsequent RNA investigations. A negative impact of OCT on the RIN value of liver samples was observed, which was independent from the applied freezing protocol and showed no impact on subsequent gene expression analysis.
PubMed: 36250023
DOI: 10.3389/fmolb.2022.876670 -
Forensic Toxicology 2023
Topics: Humans; Dry Ice; Carbon Dioxide; Ambulances; Preservation, Biological; Cadaver
PubMed: 36217319
DOI: 10.1007/s11419-022-00644-8 -
Heliyon Sep 2022Non-astringent persimmon ( Thunb.) paste is typically produced by treating astringent persimmon fruit with alcohol or dry ice (to remove tannins) followed by abrasion....
Non-astringent persimmon ( Thunb.) paste is typically produced by treating astringent persimmon fruit with alcohol or dry ice (to remove tannins) followed by abrasion. However, considering the large yield of astringent persimmons harvested in a short time, this long, laborious method has hindered the use of persimmon paste in food processing. Herein, the addition of polysaccharides was used to produce a non-astringent persimmon paste while maintaining its quality. Among the nine evaluated polysaccharides, high- (HM) and low-methoxyl (LM) pectins, carrageenan, xanthan gum, and sodium alginate exhibited high astringency removal efficiencies. No astringency recurrence was observed after freezing when HM or LM pectin, guar gum, carrageenan, or sodium alginate were added. Moreover, the addition of HM pectin, or LM pectin, or sodium alginate prevented astringency upon heating. Additionally, guar, xanthan, tara gum, or carrageenan effectively inhibited syneresis. Thus, high-quality pastes could be easily and efficiently produced using a combination of polysaccharides.
PubMed: 36185145
DOI: 10.1016/j.heliyon.2022.e10716 -
Environmental Science and Pollution... Jan 2023The aim of the research work was to explain the possibilities of application of waste activated sludge (WAS) pretreatment processes prior to anaerobic digestion...
The aim of the research work was to explain the possibilities of application of waste activated sludge (WAS) pretreatment processes prior to anaerobic digestion (mesophilic fermentation). Hydrodynamic disintegration and freezing/thawing disintegration methods were used. Based on the microbiological and parasitological analyses, a significant decrease in pathogenic bacteria, coliphages, and parasite eggs was observed. The number of bacteria analyzed (Salmonella sp., Escherichia coli, Clostridium perfringens) and coliphages were reduced from 19.3to 42.3% after hydrodynamic cavitation. A similar effect was achieved for destruction by freezing/thawing with dry ice between 7.8 and 14.9%. The effectiveness of parasite eggs reduction (Ascaris sp., Trichuris sp., Toxocara sp.) for these disintegration methods ranged from 10.7 to 29.3%. The highest results were observed for the hybrid disintegration method (hydrodynamic cavitation + dry ice disintegration) caused by a synergistic effect. Salmonella sp. in 1 g decrease about 69.7%, E. coli by 70.0%, Clostridium perfringens by 38.4%, and coliphages by 48.2%. Disruption of WAS by a hybrid method led to a reduction in the number of helminth eggs Ascaris sp. (63.8%), Trichuris sp. (64.3%), and Toxocara sp. (66.4%). After anaerobic digestion under mesophilic conditions, an additional reduction of analyzed bacterial pathogens and helminth eggs were observed. The introduction of hybrid disintegrated WAS to the fermentation chamber resulted in higher efficiency in decrease (from 1 to 23%) in comparison to the control sample (70%WAS + 30%DS (inoculum-digested sludge)).
Topics: Animals; Sewage; Anaerobiosis; Escherichia coli; Dry Ice; Toxocara; Ascaris; Trichuris; Helminths; Bacteria
PubMed: 36149557
DOI: 10.1007/s11356-022-23164-9 -
Carboxylated graphene oxide nanosheets as efficient electrodes for high-performance supercapacitors.Frontiers in Chemistry 2022In the presence of dry ice, a series of graphitic materials with carboxylated edges (ECGs) were synthesized by ball milling graphite for varied times (24, 36, and...
In the presence of dry ice, a series of graphitic materials with carboxylated edges (ECGs) were synthesized by ball milling graphite for varied times (24, 36, and 46 h). The influence of carboxylation on the physiochemical characteristics and electrochemical performance as effective electrodes for supercapacitors were assessed and compared with pure graphite. Several characterization techniques were employed to investigate into the morphology, texture, microstructure, and modification of the materials. Due to its interconnected micro-mesoporous carbon network, which is vital for fast charge-discharge at high current densities, storing static charges, facilitating electrolyte transport and diffusion, and having excellent rate performance, the ECG-46 electrode among the investigated samples achieved the highest specific capacitance of 223 F g at 0.25 A g current density and an outstanding cycle stability, with capacitance retention of 90.8% for up to 10,000 cycles. Furthermore, the symmetric supercapacitor device based on the ECG-46 showed a high energy density of 19.20 W h kg at 450.00 W kg power density. With these unique features, ball milling of graphitic material in dry ice represents a promising approach to realize porous graphitic material with oxygen functionalities as active electrodes.
PubMed: 36105311
DOI: 10.3389/fchem.2022.944793 -
Polymers Aug 2022Pd-containing precursor has been synthesized from palladium acetate and poly(vinly)silazane (Durazane 1800) in an ice bath under an argon atmosphere. The results of...
Pd-containing precursor has been synthesized from palladium acetate and poly(vinly)silazane (Durazane 1800) in an ice bath under an argon atmosphere. The results of ATR-FTIR and NMR characterizations reveal the chemical reaction between palladium acetate and vinyl groups in poly(vinyl)silazane and the hydrolyzation reaction between -Si-H and -Si-CH=CH groups in poly(vinyl)silazane. The palladium nanoparticles are in situ formed in the synthesized precursors as confirmed by XRD, XPS, and TEM. Pd- and PdSi-containing SiOCN ceramic nanocomposites are obtained by pyrolysis of the synthesized precursors at 700 °C, 900 °C-1100 °C in an argon atmosphere. The pyrolyzed nanocomposites display good catalytic activity towards the dry reforming of methane. The sample pyrolyzed at 700 °C possesses the best catalytic performance, which can be attributed to the in situ formed palladium nanoparticles and high BET surface area of about 233 m g.
PubMed: 36080545
DOI: 10.3390/polym14173470