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Biomedicines Feb 2024Wound healing requires the coordinated interaction of dermis cells, the proper deposition of extracellular matrix, re-epithelialization, and angiogenesis. Extracorporeal...
Wound healing requires the coordinated interaction of dermis cells, the proper deposition of extracellular matrix, re-epithelialization, and angiogenesis. Extracorporeal shock wave (ESW) is a promising therapeutic modality for chronic wounds. This study determined the biological mechanisms activated under ESW, facilitating the healing of pressure ulcers (PUs). A group of 10 patients with PUs received two sessions of radial ESW (300 + 100 pulses, 2.5 bars, 0.15 mJ/mm, 5 Hz). Histomorphological and immunocytochemical assessments were performed on tissue sections obtained from the wound edges before the ESW (M0) and after the first (M1) and second (M2) ESW. The proliferation index of keratinocytes and fibroblasts (Ki-67), the micro-vessels' density (CD31), and the number of myofibroblasts (α-SMA) were evaluated. The involvement of the yes-associated protein (YAP1) in sensing mechanical strain, and whether the nuclear localization of YAP1, was shown. The increased proliferative activity of epidermal cells and skin fibroblasts and the increased number of myofibroblasts, often visible as integrated cell bands, were also demonstrated as an effect of wound exposure to an ESW. The results indicate that the major skin cells, keratinocytes, and fibroblasts are mechanosensitive. They intensify proliferation and extracellular matrix remodeling in response to mechanical stress. A significant improvement in clinical wound parameters was also observed.
PubMed: 38397961
DOI: 10.3390/biomedicines12020359 -
Food Research International (Ottawa,... Mar 2024Low-temperature (9-12 °C) pulsed electric field (PEF) was investigated in milk before cream separation at different intensities (9-27 kV/cm, 66 μs, 16-28 kJ/L)...
Low-temperature (9-12 °C) pulsed electric field (PEF) was investigated in milk before cream separation at different intensities (9-27 kV/cm, 66 μs, 16-28 kJ/L) regarding its potential to render processing more sustainable, retain a high physico-chemical quality, enhance functional properties, and gently modify the structure of the milk fat globule membrane (MFGM). Cream volume per L milk were most efficiently increased by 31 % at the lowest PEF intensity in comparison to untreated milk and cream (P < 0.05). Untreated and PEF-treated milk and obtained cream were assessed with compositional (fat, protein, casein, lactose, and total solids content) and particle size distribution analyses, showing no significant differences (P ≥ 0.05) and, thus, indicating retention of 'native-like' product quality. Overrun and stability of cream, whipped for 20 and 60 s at 15000 rpm using a high-shear mixer, were improved most notably by the lowest and the highest PEF intensities, achieving up to 69 % enlarged overrun and up to 22 % higher stability, respectively (P < 0.05), than in untreated whipped cream. Protein component analyses for milk and cream were carried out by sodium dodecylsulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Noticeable differences between untreated and PEF-treated milk were not observed, but the SDS-PAGE results for cream showed noticeably different bands for some of the protein components, indicating structural changes in MFGM-, whey-, and phospho-proteins due to PEF and/or separator processing effects. More intense bands of xanthine oxidase, xanthine dehydrogenase, butyrophilin, bovine serum albumine, adipophilin (ADPH), and glycoproteins PAS6/7 were observed specifically at 21 kV/cm. Gentle electroporation of both MFGM layers by PEF was determined based on the changes in MFGM monolayer components, such as ADPH and PAS 6/7, exhibiting intensified bands. PEF intensity-dependent impact on the structure of MFGM and casein, leading to a reconfiguration of the cream matrix due to different structuring interactions among proteins, among milk fat globules, and between fat and protein components, was suggested. Overall, low-temperature PEF applied at different intensities showed great potential for gentle, efficient, and functional properties-tailored dairy processing and may also enable effective extraction of highly bioactive ingredients from dairy sources.
Topics: Animals; Caseins; Milk; Whey Proteins; Membranes; Whey
PubMed: 38395577
DOI: 10.1016/j.foodres.2024.114074 -
Journal of Conservative Dentistry and... Jan 2024Restoring an ideal proximal contact in direct Class II composite resin restorations is challenging due to polymerization shrinkage, absence of condensability of... (Review)
Review
BACKGROUND
Restoring an ideal proximal contact in direct Class II composite resin restorations is challenging due to polymerization shrinkage, absence of condensability of composite materials, thickness of matrix bands, and the use of various separation techniques, retainers, and bands.
AIMS
The aim of this study was to evaluate the proximal contact tightness that is achieved by various matrix systems used to restore a direct Class II cavity with composite resin restoration.
METHODS
A systematic review was carried out according to the PRISMA 2020 statement guidelines. The online search for the articles was done in electronic databases of MEDLINE/PubMed, Cochrane, and Google Scholar. The articles comparing different matrix systems for restoration were selected. Out of 146 articles, a total of 6 articles met the selection criteria and were included. The QUIN risk-of-bias (RoB) tool was used for assessing the study quality. The data extracted from full text articles selected for inclusion, using a standardized software (Office Excel 2013 Software, Microsoft Corporation, Redmond, WA, USA).
RESULTS AND CONCLUSION
Combination of sectional matrices and separation rings resulted in tighter proximal contact compared to other matrix systems.
PubMed: 38389748
DOI: 10.4103/JCDE.JCDE_203_23 -
Antiviral Research Apr 2024The COVID-19 pandemic has shown the need to develop effective therapeutics in preparedness for further epidemics of virus infections that pose a significant threat to...
The COVID-19 pandemic has shown the need to develop effective therapeutics in preparedness for further epidemics of virus infections that pose a significant threat to human health. As a natural compound antiviral candidate, we focused on α-dystroglycan, a highly glycosylated basement membrane protein that links the extracellular matrix to the intracellular cytoskeleton. Here we show that the N-terminal fragment of α-dystroglycan (α-DGN), as produced in E. coli in the absence of post-translational modifications, blocks infection of SARS-CoV-2 in cell culture, human primary gut organoids and the lungs of transgenic mice expressing the human receptor angiotensin I-converting enzyme 2 (hACE2). Prophylactic and therapeutic administration of α-DGN reduced SARS-CoV-2 lung titres and protected the mice from respiratory symptoms and death. Recombinant α-DGN also blocked infection of a wide range of enveloped viruses including the four Dengue virus serotypes, influenza A virus, respiratory syncytial virus, tick-borne encephalitis virus, but not human adenovirus, a non-enveloped virus in vitro. This study establishes soluble recombinant α-DGN as a broad-band, natural compound candidate therapeutic against enveloped viruses.
Topics: Mice; Animals; Humans; SARS-CoV-2; COVID-19; Dystroglycans; Pandemics; Escherichia coli; Mice, Transgenic; Antiviral Agents
PubMed: 38387750
DOI: 10.1016/j.antiviral.2024.105837 -
Scientific Reports Feb 2024In the current work, cuprous oxide (CuO) nanoparticles coated with Tween 80 were successfully synthesized via the chemical reduction method. Nanocomposites composed of...
In the current work, cuprous oxide (CuO) nanoparticles coated with Tween 80 were successfully synthesized via the chemical reduction method. Nanocomposites composed of low-density polyethylene (LDPE) and different ratios of CuO nanoparticles were fabricated by the melt mixing process. 10% of ethyl vinyl acetate (EVA) as a compatibilizing agent was added to the molten LDPE matrix and the mixing process continued until homogenous nanocomposites were fabricated. To study the influence of ionizing radiation on the fabricated samples, the prepared species were exposed to 50 and 100 kGy of gamma rays. The synthesized CuO nanoparticles were investigated by transmission electron microscopy (TEM) and X-ray diffraction (XRD). XRD and TEM analysis illustrated the successful formation of spherical CuO nanoparticles with an average size of 16.8 nm. The as-prepared LDPE/CuO nanocomposites were characterized via different techniques such as mechanical, thermal, morphological, XRD, and FTIR. Electromagnetic interference shielding (EMI) of the different nanocomposite formulations was performed as a promising application for these materials in practical life. The electromagnetic shielding effectiveness (SE) of the produced samples was measured in the X-band of the radio frequency range from 8 to 12 GHz using the vector network analyzer (VNA) and a proper waveguide. All the samples were studied before and after gamma-ray irradiation under the same conditions of pressure and temperature. The shielding effectiveness increased significantly from 25 dB for unirradiated samples to 35 dB with samples irradiated with 100 kGy, which reflects 40% enhancement in the effectiveness of the shielding.
PubMed: 38378718
DOI: 10.1038/s41598-024-54426-w -
ACS Omega Feb 2024Nonfullerene-based organic solar cells can be utilized as favorable photovoltaic and optoelectronic devices due to their enhanced life span and efficiency. In this...
Nonfullerene-based organic solar cells can be utilized as favorable photovoltaic and optoelectronic devices due to their enhanced life span and efficiency. In this research, seven new molecules were designed to improve the working efficiency of organic solar cells by utilizing a terminal acceptor modification approach. The perceived configuration-based molecules possess a lower band gap ranging from 1.95 to 2.21 eV compared to the pre-existing reference molecule (), which has a band gap of 2.23 eV. The modified molecules also exhibit higher λ values ranging from 672 to 768 nm in the gaseous and 715-839 nm in solvent phases, respectively, as compared to the () molecule, which has λ values at 673 and 719 nm in gas and chloroform medium, respectively. The ground state geometries, molecular planarity parameter, and span of deviation from the plane were analyzed to study the planarity of all of the molecules. The natural transition orbitals, the density of state, molecular electrostatic potential, noncovalent interactions, frontier molecular orbitals, and transition density matrix analysis of all studied molecules were executed to validate the optoelectronic properties of these molecules. Improved charge mobilities and dipole moments were observed, as newly designed molecules possessed lower internal reorganization energies. The open circuit voltage () of W4, W5, W6, and W7 among newly designed molecules was improved as compared to the reference molecule. These results elaborate on the superiority of these novel-designed molecules over the pre-existing () molecule as potential blocks for better organic solar cell applications.
PubMed: 38375499
DOI: 10.1021/acsomega.3c04970 -
Biophysical Reports Mar 2024The phonon spectral density plays a key role in probing the dynamical and spectral behavior of molecular aggregates. One may utilize the intimate connection between the...
The phonon spectral density plays a key role in probing the dynamical and spectral behavior of molecular aggregates. One may utilize the intimate connection between the one-phonon profile and the phonon spectral density to extract a plausible form of the spectral density of media with rich structure using advanced optical spectroscopy. The excitonic transition is normally accompanied by a broad, asymmetric phonon-side band due to the coupling to the phonons in the surrounding protein matrix present in photosynthetic complexes. The asymmetry in the one-phonon profile of a homogeneous absorption spectrum and other experiments performed on photosynthetic bacterial reaction centers (BRCs) led the Small group to employ a half-Gaussian distribution function on the red side and half-Lorentzian distribution function on the blue side of the absorption lineshape to account for the one-phonon profile asymmetrical shape and relaxation effects contributing to spectroscopy and dynamics of BRCs at hand. Different research groups successfully employed the theory of Small to simulate their photosynthetic spectral data so they could calculate the homogeneous absorption and hole-burned spectra of photosynthetic complexes. Although this report does not directly use the formulae of homogeneous absorption, hole-burning, and fluorescence line-narrowed spectra of BRCs, and photosynthetic complexes, developed by Hayes-Small, it builds on their idea of the phonon sideband asymmetric shape in deriving an accurate and computationally efficient linear electronic transition dipole moment time correlation function. Besides the compelling tractability and efficiency of this correlation function, it accounts for excitonic coupling and eliminates all the inconsistencies arising in the Hayes-Small theory.
PubMed: 38375357
DOI: 10.1016/j.bpr.2024.100146 -
ACS Organic & Inorganic Au Feb 2024Spinel oxides with the general formula ABO comprise a large family of compounds covering a very wide range of band-gap values (1 eV < < 8 eV) as a function of the...
Spinel oxides with the general formula ABO comprise a large family of compounds covering a very wide range of band-gap values (1 eV < < 8 eV) as a function of the nature of the metallic cations A and B. Owing to this, the physical properties of these materials have been largely exploited both from a fundamental point of view, for their variable electronic properties, and for their possible use in numerous engineering applications. Herein, the modeling of ZnAlO, ZnGaO, MgAlO, and MgGaO cubic spinel oxides has been carried out by using the semiempirical approach based on the difference of electronegativity between oxygen and the average electronegativity of cations present in the oxides. The results of recent theoretical extensions of our semiempirical approach to ternary and quaternary oxides have been tested for spinel oxides with metallic ions occupying both octahedrally and tetrahedrally coordinated sites in different ratios. A detailed analysis of the experimental band-gap values and comparison with the theoretically estimated values has been carried out for ternary ZnAlO, ZnGaO, MgAlO, and MgGaO spinels as well as for double spinels Mg(AlGa)O and Zn(AlGa)O, and quaternary mixed oxides (ZnMg)AlO and (ZnMg)GaO. The wide range of band-gap values reported in the literature for simple or double spinels has been related to the different preparation methods affecting the grain dimension of crystalline spinel samples as well as to the presence of crystallographic defects and/or impurities in the spinel matrix. The good agreement between experimental band-gap values and the theoretical ones strongly supports the use of our semiempirical approach in the area of band-gap engineering of new materials.
PubMed: 38344014
DOI: 10.1021/acsorginorgau.3c00030 -
Cancers Jan 2024Esophageal carcinoma (EC) is a prominent contributor to cancer-related mortality since it lacks discernible features in its first phases. Multiple studies have shown...
Assessment of Narrow-Band Imaging Algorithm for Video Capsule Endoscopy Based on Decorrelated Color Space for Esophageal Cancer: Part II, Detection and Classification of Esophageal Cancer.
Esophageal carcinoma (EC) is a prominent contributor to cancer-related mortality since it lacks discernible features in its first phases. Multiple studies have shown that narrow-band imaging (NBI) has superior accuracy, sensitivity, and specificity in detecting EC compared to white light imaging (WLI). Thus, this study innovatively employs a color space linked to décor to transform WLIs into NBIs, offering a novel approach to enhance the detection capabilities of EC in its early stages. In this study a total of 3415 WLI along with the corresponding 3415 simulated NBI images were used for analysis combined with the YOLOv5 algorithm to train the WLI images and the NBI images individually showcasing the adaptability of advanced object detection techniques in the context of medical image analysis. The evaluation of the model's performance was based on the produced confusion matrix and five key metrics: precision, recall, specificity, accuracy, and F1-score of the trained model. The model underwent training to accurately identify three specific manifestations of EC, namely dysplasia, squamous cell carcinoma (SCC), and polyps demonstrates a nuanced and targeted analysis, addressing diverse aspects of EC pathology for a more comprehensive understanding. The NBI model effectively enhanced both its recall and accuracy rates in detecting dysplasia cancer, a pre-cancerous stage that might improve the overall five-year survival rate. Conversely, the SCC category decreased its accuracy and recall rate, although the NBI and WLI models performed similarly in recognizing the polyp. The NBI model demonstrated an accuracy of 0.60, 0.81, and 0.66 in the dysplasia, SCC, and polyp categories, respectively. Additionally, it attained a recall rate of 0.40, 0.73, and 0.76 in the same categories. The WLI model demonstrated an accuracy of 0.56, 0.99, and 0.65 in the dysplasia, SCC, and polyp categories, respectively. Additionally, it obtained a recall rate of 0.39, 0.86, and 0.78 in the same categories, respectively. The limited number of training photos is the reason for the suboptimal performance of the NBI model which can be improved by increasing the dataset.
PubMed: 38339322
DOI: 10.3390/cancers16030572 -
Foods (Basel, Switzerland) Jan 2024The main objective of this work was to evaluate the combined effect of a biotechnology process, based on selected yeast strains, and a high-pressure homogenization (HPH)...
The main objective of this work was to evaluate the combined effect of a biotechnology process, based on selected yeast strains, and a high-pressure homogenization (HPH) treatment on the microbiological quality, structural organization of proteins, chitin content, and antioxidant activity of a mixture of cricket powder () and water. Compared to untreated samples, the cricket matrix treated with HPH four times at 180 MPa promoted the growth of the inoculated and strains. HPH did not affect the concentration of chitin; however, the combination with microorganisms tended to reduce the content. Although the antioxidant activity increased from 0.52 to 0.68 TAC mM/TE after a 48 h incubation in the control, it was further improved by the combination of HPH and metabolism, reaching a value of 0.77 TAC mM/TE. The combination of the two approaches also promoted a reduction in the intensity of bands with molecular weights between 31 and 21.5 kDa in favor of bands with a lower molecular weight. In addition, HPH treatment reduced the number of accessible thiols, suggesting protein structure changes that may further impact the technological properties of cricket powder.
PubMed: 38338584
DOI: 10.3390/foods13030449