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Veterinary Research Forum : An... 2023is a Gram-negative, facultative anaerobic bacillus of family, and it is an uncommon pathogen in domestic animals. To date, five cases were reported including two dogs,...
is a Gram-negative, facultative anaerobic bacillus of family, and it is an uncommon pathogen in domestic animals. To date, five cases were reported including two dogs, two cattle, and a goat. is the second common bacterial agent after the . subsp in equine pneumonia cases. The present report describes the isolation of . from lungs and spleen of a 10-year-old Arabian horse (May 08, 2022) at post-mortem examination being co-infected with Clinical and pathological findings included bilateral nasal discharge, conjunctivitis, sternal recumbency, severe diffuse necrosuppurative rhinitis, multi-focal fibrinopurulent pneumonia and purulent lymphadenitis. Polymerase chain reaction assays showed no viral nucleic acids of equid alphaherpesvirus (EHV) 1, EHV-4, equine arteritis virus and equine papilloma virus. The antibiogram test revealed that the isolate was sensitive to several antibiotics except colistin. Taken together, the present report documents the first isolation of . from lungs and spleen of a horse; hence, experimental studies are needed to clarify the pathogenity and pathogenesis of . .
PubMed: 38174095
DOI: 10.30466/vrf.2023.1987898.3760 -
Euro Surveillance : Bulletin Europeen... Dec 2022IntroductionMeat can be a vehicle for food-borne transmission of antimicrobial resistant bacteria and antimicrobial resistance genes. The occurrence of extended-spectrum...
Finding of extended-spectrum beta-lactamase (ESBL)-producing Enterobacterales in wild game meat originating from several European countries: predominance of producing CTX-M-1, November 2021.
IntroductionMeat can be a vehicle for food-borne transmission of antimicrobial resistant bacteria and antimicrobial resistance genes. The occurrence of extended-spectrum beta-lactamase (ESBL) producing Enterobacterales has been observed in meat from livestock production but has not been well studied in meat from wild game.AimWe aimed to investigate, particularly in central Europe, to what extent ESBL-producing Enterobacterales may be present in wild game meat.MethodsA total of 111 samples of different types of game meat supplied by butchers, hunters, retail stores and a large game-processing establishment in Europe were screened for ESBL-producing Enterobacterales using a selective culture medium. Isolates were genotypically and phenotypically characterised.ResultsThirty-nine samples (35% of the total) yielded ESBL-producing Enterobacterales, with most (35/39) supplied by the game-processing establishment. Isolates included 32 , 18 and one . PCR screening identified (n = 31), (n = 8), (n = 4), (n = 3), (n = 1), (n = 1), (n = 1), and (n = 2). Most belonged to phylogenetic group A (n = 7) or B1 (n = 9), but several isolates belonged to extraintestinal pathogenic (ExPEC) sequence types (ST)58 (n = 4), ST68 (n = 1) and ST540 (n = 1). Whole genome sequencing of six selected isolates localised on megaplasmids in four and on IncN_1 plasmids in one and one . Forty-eight isolates (94%) exhibited a multidrug-resistance phenotype.ConclusionWe found a high occurrence of ESBL-producing Enterobacterales in wild game meat, suggesting wildlife habitat pollution and possible microbial contamination events occurring during skinning or cutting carcasses.
Topics: Humans; Escherichia coli; Escherichia coli Infections; Phylogeny; beta-Lactamases; Enterobacteriaceae; Meat; Europe
PubMed: 36695441
DOI: 10.2807/1560-7917.ES.2022.27.49.2200343 -
Microorganisms Apr 2022, a member of the family of , although isolated widely in nature, rarely causes infections in humans. Herein, we report a case of isolation of from pigtail end culture,...
BACKGROUND
, a member of the family of , although isolated widely in nature, rarely causes infections in humans. Herein, we report a case of isolation of from pigtail end culture, urine culture and blood culture in a 76-year-old patient.
OBJECTIVE
To systematically address all the relevant information regarding through literature.
METHODS
We searched PubMed and Scopus databases up to January 2022 and performed a qualitative synthesis of published articles reporting infection from in humans.
RESULTS
We identified 25 records on PubMed and 43 additional records on Scopus. After removing duplicates, we examined in detail 15 articles. Ten studies with a total of 17 cases were included in our systematic review. Nine studies described isolated case reports, while 1 study described 8 cases. The origin of the infection was the alimentary tract in 9 cases, gallbladder in 4 cases, peritoneal cavity in 2 cases, respiratory tract in 1 case and hemodialysis catheter insertion site in 1 case. In 3 of the aforementioned cases was also isolated in blood cultures.
CONCLUSION
Physicians should be aware that can be present in multiple clinical specimens and that the antibiotic resistance profile of the isolates may pose significant challenges.
PubMed: 35630337
DOI: 10.3390/microorganisms10050892 -
Frontiers in Microbiology 2022Sliced cooked ham packaged in a modified atmosphere is a popular ready-to-eat product, subjected to abundant microbial contamination throughout its shelf life that can...
Sliced cooked ham packaged in a modified atmosphere is a popular ready-to-eat product, subjected to abundant microbial contamination throughout its shelf life that can lead to deterioration of both sensorial properties and safety. In this study, the microbial load and the chemical-physical features of cooked ham of five producers were monitored for a period of 12 days after the opening of the packages (i.e., the secondary shelf life), during which the products were stored in a domestic refrigerator at 5.2 ± 0.6°C. The sensorial properties presented a perceivable decay after 8 days and became unacceptable after 12 days. High-performance liquid chromatography analysis and solid-phase microextraction coupled with gas chromatography profiling of volatile metabolites indicated that lactic acid, ethanol, acetic acid, acetoin, 3-methyl-1-butanol, and 2-3 butanediol were the main metabolites that characterized the evolution of the analyzed cooked ham. The microbiota was monitored by 16S ribosomal RNA gene profiling and culture-dependent techniques. Already at the opening of packages, all the products presented high microbial load, generally dominated by lactic acid bacteria, with evident differences among the products. The increase of lactic acid bacteria somehow protected samples from abundant contamination by other bacteria, concurring with the evolution of more safe products. This role was exerted by numerous , , and species, among which the most frequently detected were , , , and Some products presented more complex communities that encompassed Proteobacteria such as , , , and less frequently , , and . Opportunistic pathogenic bacteria such as and V sp. were found in small quantities. The yeasts and occurred already at 0 days, whereas various species of (, , , and ) were abundant only after 12 days. These results indicated that the microbiological contaminants overgrowing during the secondary shelf life did not derive from environmental cross-contamination at the opening of the tray but were already present when the packages were opened, highlighting the phases of production up to the packaging as those crucial in managing the safety risk associated to this product.
PubMed: 35350621
DOI: 10.3389/fmicb.2022.842390 -
Animals : An Open Access Journal From... Jan 2022is an Enterobacteriaceae with unclarified dispersion and pathogenicity. During an ongoing investigation about antimicrobial resistance in Greece, the occurrence of was...
is an Enterobacteriaceae with unclarified dispersion and pathogenicity. During an ongoing investigation about antimicrobial resistance in Greece, the occurrence of was evaluated among wild birds and humans. A total of 445 wild bird and 2000 human fecal samples were collected and screened for the presence of the organism. Subsequently, all strains were phenotypically and molecularly characterized regarding their antimicrobial resistance characteristics. Four were isolated from a common pheasant (), two Eurasian magpies () and a great white-fronted goose (). Among these four strains, the three latter presented resistance to 3rd generation cephalosporins, were phenotypically confirmed to produce ESBLs and were found to harbor . The three ESBL isolates additionally exhibited resistance to tetracyclines, while resistance to aminoglycosides was detected in two of them and to trimethoprim/sulfamethoxazole in one. No strains were retrieved from the human samples tested. This is the first report that provides evidence of dissemination among wild birds in Greece, describing CTX-M-1 production in multidrug resistant wild birds' isolates of this bacterial species.
PubMed: 35158664
DOI: 10.3390/ani12030340 -
Veterinaria Italiana May 2021Bacterial urinary tract infection (UTI) is a common condition affecting dogs. Urine culture and antimicrobial susceptibility test, associated with the identification of...
Bacterial urinary tract infection (UTI) is a common condition affecting dogs. Urine culture and antimicrobial susceptibility test, associated with the identification of underlying cause, are of primary importance in order to select a correct treatment, especially in presence of comorbidities. Two cases of immunecompromised dogs affected by urinary tract infection (UTI) have been described: the first, probably immunosuppressed due to old age, was in poor body condition, with severe odontolithiasis and periodontitis; the second was affected by chronic kidney disease in advanced stage. Urine cultures isolated two rare and atypical pathogens, Moellerella wisconsensis and Brevundimonas vesicularis, both showing sensitivity versus floroquinolones which were selected for the treatment. After a 4 weeks treatment, a second culture demonstrated the resolution of infection in both cases, in absence of clinical signs.To date neither of the two bacteria have been reported as cause of UTI in dog.
Topics: Animals; Anti-Bacterial Agents; Caulobacteraceae; Diagnosis, Differential; Dog Diseases; Dogs; Female; Gammaproteobacteria; Immunocompromised Host; Male; Pedigree; Urinalysis; Urinary Tract Infections
PubMed: 34313102
DOI: 10.12834/VetIt.2110.12149.1 -
Journal of Infection in Developing... Feb 2020Infections caused by carbapenemase-producing Enterobacteriaceae have become a major threat to public health, worldwide. Here we report clinically significant NDM-1 and...
Infections caused by carbapenemase-producing Enterobacteriaceae have become a major threat to public health, worldwide. Here we report clinically significant NDM-1 and VIM-1 producing Moellerella wisconsensis which has not yet been described in the literature; this is the first report of M. wisconsensis strain harbouring blaNDM-1 and blaVIM-1, recovered from the rectal swab of a low birth weight female child admitted in NICU of the north Indian tertiary care hospital. A plasmid of IncW incompatibility with size of 154 kb was observed in AK-92 strain.
Topics: Bacterial Proteins; Drug Resistance, Multiple, Bacterial; Enterobacteriaceae Infections; Female; Gammaproteobacteria; Humans; India; Infant, Newborn; Intensive Care Units, Neonatal; Plasmids; beta-Lactamases
PubMed: 32146459
DOI: 10.3855/jidc.10969 -
PloS One 2018Social wasps are a major pest in many countries around the world. Pathogens may influence wasp populations and could provide an option for population management via...
Social wasps are a major pest in many countries around the world. Pathogens may influence wasp populations and could provide an option for population management via biological control. We investigated the pathology of nests of apparently healthy common wasps, Vespula vulgaris, with nests apparently suffering disease. First, next-generation sequencing and metatranscriptomic analysis were used to examine pathogen presence. The transcriptome of healthy and diseased V. vulgaris showed 27 known microbial phylotypes. Four of these were observed in diseased larvae alone (Aspergillus fumigatus, Moellerella wisconsensis, Moku virus, and the microsporidian Vavraia culicis). Kashmir Bee Virus (KBV) was found to be present in both healthy and diseased larvae. Moellerella wisconsensis is a human pathogen that was potentially misidentified in our wasps by the MEGAN analysis: it is more likely to be the related bacteria Hafnia alvei that is known to infect social insects. The closest identification to the putative pathogen identified as Vavraia culicis was likely to be another microsporidian Nosema vulgaris. PCR and subsequent Sanger sequencing using published or our own designed primers, confirmed the identity of Moellerella sp. (which may be Hafnia alvei), Aspergillus sp., KBV, Moku virus and Nosema. Secondly, we used an infection study by homogenising diseased wasp larvae and feeding them to entire nests of larvae in the laboratory. Three nests transinfected with diseased larvae all died within 19 days. No pathogen that we monitored, however, had a significantly higher prevalence in diseased than in healthy larvae. RT-qPCR analysis indicated that pathogen infections were significantly correlated, such as between KBV and Aspergillus sp. Social wasps clearly suffer from an array of pathogens, which may lead to the collapse of nests and larval death.
Topics: Animals; Gene Expression Profiling; Host-Pathogen Interactions; Larva; Metagenome; Metagenomics; Microbiota; Phylogeny; Wasps
PubMed: 30596703
DOI: 10.1371/journal.pone.0209589 -
Clinical Microbiology and Infection :... Dec 2009
Topics: Aged, 80 and over; Bacteriological Techniques; Blood; Cholecystitis, Acute; Culture Media; Enterobacteriaceae; Enterobacteriaceae Infections; Humans; Male
PubMed: 19732083
DOI: 10.1111/j.1469-0691.2009.03046.x -
Journal of Clinical Microbiology Apr 1984The name Moellerella wisconsensis is proposed for a group of the family Enterobacteriaceae previously called enteric group 46. The species name, wisconsensis, was coined...
The name Moellerella wisconsensis is proposed for a group of the family Enterobacteriaceae previously called enteric group 46. The species name, wisconsensis, was coined because six of the nine strains were isolated in Wisconsin. M. wisconsensis strains were negative for indole production, Voges-Proskauer, H2S production, urea, phenylalanine deaminase, lysine and ornithine decarboxylases, arginine dihydrolase, gas production from D-glucose, acid production from trehalose, and motility; the strains were positive for methyl red, citrate (Simmons), and acid production from lactose and raffinose and resistant to colistin. DNAs from five strains of M. wisconsensis were highly related (80 to 93% in reactions assayed on hydroxyapatite at 60 degrees C and 78 to 97% at 75 degrees C) to 32P-labeled DNA of the proposed type strain (CDC 2896-78, ATCC 35017). Labeled DNA from this type strain was only 2 to 32% related (at 60 degrees C) to DNA from 49 strains of named and unnamed species of Enterobacteriaceae. Eight of nine M. wisconsensis strains were isolated from human stool samples. Clinical information on one strain was available, and it was found to be associated with a case of diarrhea. On MacConkey agar, colonies of M. wisconsensis were bright red with precipitated bile around them and thus were indistinguishable from Escherichia coli colonies. Future studies should focus on the isolation of this new organism and its relationship to human disease.
Topics: Adult; Anti-Bacterial Agents; Child, Preschool; DNA, Bacterial; Diarrhea; Enterobacteriaceae; Feces; Female; Gastroenteritis; Humans; Male; Microbial Sensitivity Tests; Middle Aged; Nucleic Acid Hybridization
PubMed: 6715516
DOI: 10.1128/jcm.19.4.460-463.1984