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Frontiers in Medicine 2024Prior research underscores the significance of paraspinal muscles in maintaining spinal stability. This study aims to investigate the predictive value of paraspinal...
OBJECTIVE
Prior research underscores the significance of paraspinal muscles in maintaining spinal stability. This study aims to investigate the predictive value of paraspinal muscle parameters for the occurrence of new vertebral compression fractures (NVCF) following percutaneous vertebroplasty (PVP) or percutaneous kyphoplasty (PKP) in patients with osteoporotic vertebral compression fractures (OVCF).
METHODS
Retrospectively collected data from October 2019 to February 2021 (internal validation, = 235) and March 2021 to November 2021 (external validation, = 105) for patients with OVCF treated with PVP/PKP at our institution. They were randomly divided into training (188 cases) and validation groups (47 cases) at an 8:2 ratio. Lasso regression and multivariable logistic regression identified independent risk factors in the training set, and a Nomogram model was developed. Accuracy was assessed using receiver operating characteristic curves (ROC), calibration was evaluated with calibration curves and the Hosmer-Lemeshow test, and clinical utility was analyzed using decision curve analysis (DCA) and clinical impact curve (CIC).
RESULTS
Surgical approach, spinal computed tomography (CT) values, and multifidus skeletal muscle index (SMI) are independent predictors of postoperative NVCF in OVCF patients. A Nomogram model, based on the identified predictors, was developed and uploaded online. Internal validation results showed area under the curve (AUC) values of 0.801, 0.664, and 0.832 for the training set, validation set, and external validation, respectively. Hosmer-Lemeshow goodness-of-fit tests ( = 7.311-14.474, = 0.070-0.504) and calibration curves indicated good consistency between observed and predicted values. DCA and CIC demonstrated clinical net benefit within risk thresholds of 0.06-0.84, 0.12-0.23, and 0.01-0.27. At specificity 1.00-0.80, the partial AUC (0.106) exceeded that at sensitivity 1.00-0.80 (0.062).
CONCLUSION
Compared to the spinal CT value, the multifidus SMI has certain potential in predicting the occurrence of NVCF. Additionally, the Nomogram model of this study has a greater negative predictive value.
PubMed: 38813387
DOI: 10.3389/fmed.2024.1379078 -
Sports Medicine International Open 2024Mean propulsive velocity (MPV) has been associated with neuromuscular fatigue; however, its suitability for strength training in Paralympic powerlifting (PP) remains...
Mean propulsive velocity (MPV) has been associated with neuromuscular fatigue; however, its suitability for strength training in Paralympic powerlifting (PP) remains uncertain. The objective of this work was to evaluate the MPV in two training methods (traditional-TRAD and eccentric-ECC). Eleven PP athletes were evaluated pre, during the intervention and post intervention at a load of 80% of the 1RM for TRAD and 110%-80% of 1 RM for ECC. The results demonstrated that there was no significant neuromuscular fatigue for the TRAD (~5% performance loss), as well as no significant decline in MPV during the intervention. For the ECC, there is a significant reduction in MPV before and after training (~12% loss of performance). A difference between TRAD and ECC after the intervention was also identified (0.87 m/s±0.22, 95% CI 0.72-1.02 vs. 0.72±0.20, 95% CI 0.59-0.86 p=0.042, F(3.30)=10.190, η2p=0.505 - very high effect). During the intervention for ECC, no significant decline in MPV was observed. The results of this study suggest that the mechanical indices of MPV do not seem to be effective indicators of neuromuscular fatigue in the sample studied or in the context of this specific training regime, being more indicated as a control of training volume.
PubMed: 38812958
DOI: 10.1055/a-2207-7922 -
Frontiers in Sports and Active Living 2024The objective of our study was to examine, in addition to using the TOCA Football System tool and training method, the effect of a 10-week intervention on elite youth...
INTRODUCTION
The objective of our study was to examine, in addition to using the TOCA Football System tool and training method, the effect of a 10-week intervention on elite youth athletes in terms of their sport-specific motor skills and anthropometric variables.
METHODS
The study covered a group of 32 young players practicing football (U14) (13.45 ± 0.64 years). The junior U14 footballers were randomly assigned to an intervention or TOCA group (TG, = 15, 13.25 ± 0.58 years) and a control group (CG, = 17, 13.63 ± 0.66 years). Before starting the test, we performed full anthropometric measurements and assessed the sample's agility with and without the ball and their sport-specific endurance. The measurements were then repeated after the 10-week intervention.
RESULTS
Within-group analysis showed significant improvements in muscle mass ( < 0.001), sport-specific endurance ( < 0.001), ( < 0.004) and agility (in TG) both with and without the ball ( = 0.002), ( = 0.004) however, we did not find a significant change in body fat percentage in either group ( = 0.988, = 0.288). In the CG, "agility with the ball" changed significantly only ( = 0.023). In the between-group analysis with a repeated-measures analysis of variance (mixed-design ANOVA), there was no significant interaction in any performance variables. The main findings of this study indicate that a TOCA Football training program in addition to normal training during the in-season period does not produce additional effects in anthropometric factors, sport-specific endurance and agility performance with the ball (dribbling) and without the ball in comparison with the control condition.
DISCUSSION
From a practical point of view, the presented anthropometric and physical profiles of players can be useful for football coaches in optimizing soccer training. Overall, it also can be concluded that the device can be safely used in the sensitive age group in terms of the development of motor skills since we did not find any negative effects during the use of the device in terms of the parameters we examined. In addition to the expansion of the number of elements and the inclusion of other age groups, it is advisable to carry out further complex tests, as the TOCA Football System offers many research opportunities.
PubMed: 38812892
DOI: 10.3389/fspor.2024.1339768 -
Frontiers in Cardiovascular Medicine 2024Vascular endothelial cells play an important role in regulating peripheral circulation by modulating arterial tone in the microvasculature. Elevated intracellular Ca... (Review)
Review
Vascular endothelial cells play an important role in regulating peripheral circulation by modulating arterial tone in the microvasculature. Elevated intracellular Ca levels are required in endothelial cells to induce smooth muscle relaxation via endothelium-dependent mechanisms such as nitric oxide production, prostacyclin, and endothelial cell hyperpolarization. It is well established that exogenous administration of acetylcholine can increase intracellular Ca concentrations, followed by endothelium-dependent vasodilation. Although endogenous acetylcholine's regulation of vascular tone remains debatable, recent studies have reported that endogenously derived acetylcholine, but not neuronal cell-derived acetylcholine, is a key modulator of endothelial cell function. In this minireview, we summarize the current knowledge of the non-neuronal cholinergic system (NNCS) in vascular function, particularly vascular endothelial cell function, which contributes to blood pressure regulation. We also discuss the possible pathophysiological impact of endothelial NNCS, which may induce the development of vascular diseases due to endothelial dysfunction, and the potential of endothelial NNCS as a novel therapeutic target for endothelial dysfunction in the early stages of metabolic syndrome, diabetes, and hypertension.
PubMed: 38812748
DOI: 10.3389/fcvm.2024.1388528 -
Turkish Journal of Medical Sciences 2024Calpainopathy, also known as limb-girdle muscular dystrophy recessive type 1, is a progressive muscle disorder that impacts the muscles around the hips and shoulders....
BACKGROUND AND AIM
Calpainopathy, also known as limb-girdle muscular dystrophy recessive type 1, is a progressive muscle disorder that impacts the muscles around the hips and shoulders. The disease is caused by defects in the gene and can be inherited in both recessive and dominant forms. In this retrospective study, we aimed to evaluate the clinical and molecular results of our patients with calpainopathy and to examine the variants in Turkish and global populations.
MATERIALS AND METHODS
Molecular analyses were performed using the next-generation sequencing (NGS) method. variants were identified through the examination of various databases.
RESULTS
In this retrospective study, the cohort consisted of seven patients exhibiting the (NM_000070.3) mutation and a phenotype compatible with calpainopathy at a single center in Türkiye. All patients displayed high CK levels and muscle weakness. We report a novel missense c.2437G>A variant that causes the autosomal dominant form of calpainopathy. Interestingly, the muscle biopsy report for the patient with the novel mutation indicated sarcoglycan deficiency. Molecular findings for the remaining individuals in the cohort included a compound heterozygous variant (frameshift and missense), one homozygous nonsense, one homozygous intronic deletion, and three homozygous missense variants. The most common variant in the Turkish population was c.550del. In both populations, pathogenic variants were most frequently located in exon 21, according to exon length. Variants were stochastically distributed based on consequences in CAPN3 domains.
CONCLUSION
Therefore, the NGS method proves highly effective in diagnosing rare diseases characterized by clinical heterogeneity. Assessing variants based on ethnicity holds significance in the development of precise therapies.
Topics: Humans; Retrospective Studies; Muscular Dystrophies, Limb-Girdle; Turkey; Male; Calpain; Female; Muscle Proteins; Adult; Young Adult; Adolescent; Mutation; Middle Aged; Child; Cohort Studies; High-Throughput Nucleotide Sequencing
PubMed: 38812636
DOI: 10.55730/1300-0144.5769 -
Turkish Journal of Medical Sciences 2024Isokinetic strength assessment of the rotator cuff muscle is frequently applied in a variety of shoulder postures, but none of these consider muscular architecture,...
BACKGROUND/AIM
Isokinetic strength assessment of the rotator cuff muscle is frequently applied in a variety of shoulder postures, but none of these consider muscular architecture, which is one of the most important aspects of improving strength development. This study aimed to examine the test and retest reliability and validity of the muscle architecture-based position (MABP), which is 25° abduction and 20° external rotation, in healthy subjects to be able to select a better isokinetic assessment position for shoulder rotator cuff muscles.
MATERIALS AND METHODS
A total of 54 healthy males with a mean age of 21.0 ± 1.2 years and mean body mass index of 22.8 ± 1.7 kg/m completed an isokinetic measurement session. All of the tests were performed on an IsoMed 2000 isokinetic dynamometer concentrically and eccentrically for both upper limbs at 60°/s angular velocity. All of the participants completed 3 measurement sessions: the first represented the isokinetic testing and was performed in the scapular neutral position (SNP) (45° shoulder flexion and abduction), the second represented the MABP (25° abduction and 20° ER) for shoulder rotator cuff muscles, and the third represented the test and retest of the MABP.
RESULTS
The correlations between the 2 techniques for assessing concurrent validity ranged from 0.908 to 0.994. The values obtained from the MABP were higher than those obtained in the SNP. There was no systematic bias for any measurements between the MABP and the retest of the MABP (p > 0.05). The intraclass correlation coefficients representing the test and retest reliability results for each variable measured with the MABP was higher than 0.98 and this value was considered as excellent reliability.
CONCLUSION
In conclusion, the MABP can be used to assess the isokinetic strength of the rotator cuff muscles safely and confidently, with increased quantities of force being released and measurement at optimal muscle tension.
Topics: Humans; Male; Rotator Cuff; Reproducibility of Results; Muscle Strength; Young Adult; Muscle Strength Dynamometer; Range of Motion, Articular; Adult; Healthy Volunteers; Posture
PubMed: 38812618
DOI: 10.55730/1300-0144.5774 -
Frontiers in Immunology 2024Bladder cancer is a common type of cancer around the world, and the majority of patients are diagnosed with non-muscle-invasive bladder cancer (NMIBC). Although low-risk...
Bladder cancer is a common type of cancer around the world, and the majority of patients are diagnosed with non-muscle-invasive bladder cancer (NMIBC). Although low-risk NMIBC has a good prognosis, the disease recurrence rate and development of treatment-refractory disease remain high in intermediate- to high-risk NMIBC patients. To address these challenges for the treatment of NMIBC, a novel combination therapy composed of an oncolytic adenovirus (oAd) co-expressing interleukin (IL)-12, granulocyte-macrophage colony-stimulating factor (GM-CSF), and relaxin (RLX; HY-oAd) and a clinical-stage glycogen synthase kinase (GSK)-3β inhibitor (9-ING-41; elraglusib) was investigated in the present report. Our findings demonstrate that HY-oAd and 9-ING-41 combination therapy (HY-oAd+9-ING-41) exerted superior inhibition of tumor growth compared with respective monotherapy in a syngeneic NMIBC tumor model. HY-oAd+9-ING-41 induced high-level tumor extracellular matrix (ECM) degradation and a more potent antitumor immune response than the respective monotherapy. In detail, HY-oAd+9-ING-41 induced superior accumulation of intratumoral T cells, prevention of immune cell exhaustion, and induction of tumor-specific adaptive immune response compared to either monotherapy. Collectively, these results demonstrate that the combination of HY-oAd and 9-ING-41 may be a promising approach to elicit a potent antitumor immune response against bladder cancer.
Topics: Urinary Bladder Neoplasms; Tumor Microenvironment; Animals; Adenoviridae; Oncolytic Virotherapy; Oncolytic Viruses; Mice; Humans; Glycogen Synthase Kinase 3 beta; Cell Line, Tumor; Combined Modality Therapy; Female
PubMed: 38812516
DOI: 10.3389/fimmu.2024.1360436 -
Skeletal Muscle May 2024Intramuscular fat (IMAT) infiltration, pathological adipose tissue that accumulates between muscle fibers, is a shared hallmark in a diverse set of diseases including... (Comparative Study)
Comparative Study
Intramuscular fat (IMAT) infiltration, pathological adipose tissue that accumulates between muscle fibers, is a shared hallmark in a diverse set of diseases including muscular dystrophies and diabetes, spinal cord and rotator cuff injuries, as well as sarcopenia. While the mouse has been an invaluable preclinical model to study skeletal muscle diseases, they are also resistant to IMAT formation. To better understand this pathological feature, an adequate pre-clinical model that recapitulates human disease is necessary. To address this gap, we conducted a comprehensive in-depth comparison between three widely used mouse strains: C57BL/6J, 129S1/SvlmJ and CD1. We evaluated the impact of strain, sex and injury type on IMAT formation, myofiber regeneration and fibrosis. We confirm and extend previous findings that a Glycerol (GLY) injury causes significantly more IMAT and fibrosis compared to Cardiotoxin (CTX). Additionally, females form more IMAT than males after a GLY injury, independent of strain. Of all strains, C57BL/6J mice, both females and males, are the most resistant to IMAT formation. In regard to injury-induced fibrosis, we found that the 129S strain formed the least amount of scar tissue. Surprisingly, C57BL/6J of both sexes demonstrated complete myofiber regeneration, while both CD1 and 129S1/SvlmJ strains still displayed smaller myofibers 21 days post injury. In addition, our data indicate that myofiber regeneration is negatively correlated with IMAT and fibrosis. Combined, our results demonstrate that careful consideration and exploration are needed to determine which injury type, mouse model/strain and sex to utilize as preclinical model especially for modeling IMAT formation.
Topics: Animals; Male; Female; Mice, Inbred C57BL; Regeneration; Muscle, Skeletal; Mice; Adipose Tissue; Fibrosis; Disease Models, Animal; Sex Characteristics; Species Specificity; Glycerol; Mice, 129 Strain
PubMed: 38812056
DOI: 10.1186/s13395-024-00344-4 -
Scientific Reports May 2024Intramuscular fat (IMF) in pork holds significant importance for economic performance within the pig industry and dietary calcium supplementation enhances the...
Intramuscular fat (IMF) in pork holds significant importance for economic performance within the pig industry and dietary calcium supplementation enhances the accumulation of intramuscular fat. Additionally, calcium ions inhibit translation and reduce protein synthesis. However, the mechanism by which calcium regulates IMF deposition in muscle through translation remains largely unknown. In this study, we compared the ribosome profiles of the longissimus dorsi muscles of Duroc Landrace Large white pigs from the normal calcium (NC) group or calcium supplement (HC) group by Ribo-seq, and RNA-seq. By integrating multiple-omics analysis, we further discovered 437 genes that were transcriptionally unchanged but translationally altered and these genes were significantly enriched in the oxidative phosphorylation signaling pathway. Furthermore, experimental data showed that inhibiting the expression of COX10 and mtND4L increased triglyceride accumulation in C2C12 cells, providing new targets for intramuscular fat deposition. Finally, this work links dietary calcium, translation regulation and IMF deposition, providing a new strategy for both meat quality and economic performance within the pig industry.
Topics: Animals; Muscle, Skeletal; Swine; Calcium, Dietary; Adipose Tissue; Dietary Supplements; Mice; Protein Biosynthesis; Triglycerides; Calcium
PubMed: 38811812
DOI: 10.1038/s41598-024-62986-0 -
Scientific Reports May 2024The aim of the present study was to investigate the variations in individual muscle stiffness across different maturation stages (i.e., peak height velocity [PHV]) in...
The aim of the present study was to investigate the variations in individual muscle stiffness across different maturation stages (i.e., peak height velocity [PHV]) in elite youth soccer players and to explore the associations between lower limb muscle stiffness and performance in sprinting (10, 20, and 40 m sprint), maneuverability (9-3-6-3-9 m sprint test), and jumping (countermovement jump [CMJ]). A total of 131 elite youth soccer players aged 12-18 years, volunteered to participate in the study and were divided into pre-PHV (n = 21), mid-PHV (n = 33), and post-PHV (n = 80). Muscle stiffness of the rectus femoris (RF) and biceps femoris (BF) muscles was assessed using a MyotonPRO. Results showed that players in the pre-PHV stage had lower stiffness in the BF and RF muscles compared to mid-PHV (p < 0.001; effect size [ES] = moderate to large) and post-PHV players (p < 0.001; ES = moderate to large). It was also observed that the mid-PHV group had lower stiffness levels in their RF muscle compared to the post-PHV group (p < 0.001; ES = small). Significant correlations were found between BF and RF stiffness and sprint (p < 0.001) and maneuverability (p < 0.001) performance. RF stiffness showed a significant positive correlation with CMJ (p < 0.05), suggesting that greater lower body stiffness is beneficial for athletic performance in youth soccer players. The findings highlighting the importance of considering training methods that increase muscular stiffness, particularly in relation to the RF muscle, to optimize athletic performance.
Topics: Humans; Soccer; Adolescent; Athletic Performance; Child; Male; Muscle, Skeletal; Athletes; Running
PubMed: 38811805
DOI: 10.1038/s41598-024-63224-3