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Wellcome Open Research 2024We present a genome assembly from an individual male (the Summer Chafer; Arthropoda; Insecta; Coleoptera; Scarabaeidae). The genome sequence is 1,584.1 megabases in...
We present a genome assembly from an individual male (the Summer Chafer; Arthropoda; Insecta; Coleoptera; Scarabaeidae). The genome sequence is 1,584.1 megabases in span. Most of the assembly is scaffolded into 11 chromosomal pseudomolecules, including the X and Y sex chromosomes. The mitochondrial genome has also been assembled and is 19.29 kilobases in length.
PubMed: 38784435
DOI: 10.12688/wellcomeopenres.21100.1 -
ZooKeys 2024(Marshall, 1888) is redescribed and illustrated based on the holotype of Marshall, 1888 and on recently collected material from its type locality (Sicily, Italy)....
(Marshall, 1888) is redescribed and illustrated based on the holotype of Marshall, 1888 and on recently collected material from its type locality (Sicily, Italy). Previous host records for this species are unreliable. Here, the host of , the rare ptinid beetle Fairmaire, 1875, is recorded for the first time, having been reared in a historic library in Palermo, Italy. is compared with the similar species (= ) Marshall, 1888, which was described in the same monograph from Corsica (France), and it is also redescribed and illustrated. Docavo Alberti, 1960, is synonymised under (Marshall, 1888), and (= ) Giraud var. flava Fahringer, 1931, is a junior synonym of Rohwer, 1925. A key for determination of the Western Palaearctic species with a striate vertex is provided and the distributions of and are discussed.
PubMed: 38779585
DOI: 10.3897/zookeys.1201.118549 -
ZooKeys 2024A new genus of malachiine Melyridae, , is described based on several male-specific characters, along with description of its type species, , from China. A new...
A new genus of malachiine Melyridae, , is described based on several male-specific characters, along with description of its type species, , from China. A new combination, (Champion, 1921), , is proposed after examining the type specimen; this species is newly recorded from China. A key to the genera of Chinese Apalochrini is provided for the first time.
PubMed: 38779583
DOI: 10.3897/zookeys.1201.123141 -
Wellcome Open Research 2024We present a genome assembly from an individual female (metallic wood-boring beetle; Arthropoda; Insecta; Coleoptera; Buprestidae). The genome sequence is 292.3...
We present a genome assembly from an individual female (metallic wood-boring beetle; Arthropoda; Insecta; Coleoptera; Buprestidae). The genome sequence is 292.3 megabases in span. Most of the assembly is scaffolded into 10 chromosomal pseudomolecules, including the X sex chromosome. The mitochondrial genome has also been assembled and is 15.91 kilobases in length.
PubMed: 38779150
DOI: 10.12688/wellcomeopenres.20877.1 -
Wellcome Open Research 2023We present a genome assembly from an individual female (a rove beetle; Arthropoda; Insecta; Coleoptera; Staphylinidae). The genome sequence is 870.5 megabases in span....
We present a genome assembly from an individual female (a rove beetle; Arthropoda; Insecta; Coleoptera; Staphylinidae). The genome sequence is 870.5 megabases in span. Most of the assembly is scaffolded into 10 chromosomal pseudomolecules, including the X sex chromosome. The mitochondrial genome has also been assembled and is 20.71 kilobases in length.
PubMed: 38779053
DOI: 10.12688/wellcomeopenres.20338.1 -
BMC Ecology and Evolution May 2024Dorcus stag beetles in broad sense are one of the most diverse group in Lucanidae and important saproxylic insects playing a crucial role in nutrient recycling and...
BACKGROUND
Dorcus stag beetles in broad sense are one of the most diverse group in Lucanidae and important saproxylic insects playing a crucial role in nutrient recycling and forest biomonitoring. However, the dazzling morphological differentiations have caused numerous systematic confusion within the big genus, especially the puzzlingly generic taxonomy. So far, there is lack of molecular phylogenetic study to address the chaotic situation. In this study, we undertook mitochondrial genome sequencing of 42 representative species including 18 newly-sequenced ones from Eastern Asia and reconstructed the phylogenetic framework of stag beetles in Dorcus sensu lato for the first time.
RESULTS
The mitogenome datasets of Dorcus species have indicated the variable mitogenomic lengths ranged from 15,785 to 19,813 bp. Each mitogenome contained 13 PCGs, 2 rRNAs, 22 tRNAs, and a control region, and all PCGs were under strong purifying selection (Ka/Ks < 1). Notably, we have identified the presence of a substantial intergenic spacer (IGS) between the trnA and NAD1 genes, with varying lengths ranging from 129 bp (in D. hansi) to 158 bp (in D. tityus). The mitogenomic phylogenetic analysis of 42 species showed that Eastern Asia Dorcus was monophyletic, and divided into eight clades with significant genetic distance. Four of them, Clade VIII, VII, VI and I are clustered by the representative species of Serrognathus Motschulsky, Kirchnerius Schenk, Falcicornis Séguy and Dorcus s.s. respectively, which supported their fully generic positions as the previous morphological study presented. The topology also showed the remaining clades were distinctly separated from the species of Dorcus sensu lato, which implied that each of them might demonstrate independent generic status. The Linnaeus nomenclatures were suggested as Eurydorcus Didier stat. res., Eurytrachellelus Didier stat. res., Hemisodorcus Thomson stat. res. and Velutinodorcus Maes stat. res. For Clade V, IV, III and II respectively.
CONCLUSION
This study recognized the monophyly of Dorcus stag beetles and provided a framework for the molecular phylogeny of this group for the first time. The newly generated mitogenomic data serves as a valuable resource for future investigations on lucanid beetles. The generic relationship would facilitate the systematics of Dorcus stag beetles and thus be useful for exploring their evolutionary, ecological, and conservation aspects.
Topics: Animals; Coleoptera; Phylogeny; Genome, Mitochondrial; Asia, Eastern
PubMed: 38773381
DOI: 10.1186/s12862-024-02225-2 -
ZooKeys 2024In this study, all species of the leaf-beetle genus Gressitt & Kimoto, 1963 from China are redescribed based on the reexamination of type specimens, and a new species,...
In this study, all species of the leaf-beetle genus Gressitt & Kimoto, 1963 from China are redescribed based on the reexamination of type specimens, and a new species, from Nanling Mountains, is described. A key to the three Chinese species of is provided, as well as photographs of the habiti and aedeagi of these species.
PubMed: 38766408
DOI: 10.3897/zookeys.1200.116337 -
ZooKeys 2024The group is the second species group of the New Guinean representatives of the recently described genus Shaverdo et al., 2023. The group is mainly defined by distinct...
The group is the second species group of the New Guinean representatives of the recently described genus Shaverdo et al., 2023. The group is mainly defined by distinct scale- and/or spinula-like surface structures of the dorsal sclerite of the median lobe. The species group already contains four described species and 42 new species and one subspecies treated here: , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , A checklist and identification key to New Guinean species of the group are provided and important diagnostic characters are illustrated. Data on the species distributions and habiat preferences are given.
PubMed: 38765729
DOI: 10.3897/zookeys.1201.116131 -
Journal of Insect Physiology Jun 2024The impacts of climate change on the development of insects are of great concern due to potential alterations in population dynamics and pest pressure. The carrot...
The impacts of climate change on the development of insects are of great concern due to potential alterations in population dynamics and pest pressure. The carrot weevil, Listronotus oregonensis, is a major agricultural pest, and its development is influenced by temperature and photoperiod. In this study, our aim was to investigate the impact of temperature increases on the voltinism and reproductive diapause of the carrot weevil under field conditions and bioclimatic models. Field observations were conducted over two growing seasons using structures that allowed for temperature increases. The developmental stages of the carrot weevil, including female reproductive status, oviposition and larval stage, were monitored weekly to measure the proportion of individuals undergoing an additional generation. Concurrently, bioclimatic models were used to simulate the probability of a second generation under current (1981-2010) and future (2041-2070) climates, considering a lower and a higher change in emission scenarios. Results showed that rising temperatures led to an increase in the proportion of carrot weevils undergoing inhibition of the reproductive diapause and a higher number of eggs laid in the field. The models indicated a substantial rise in the probability of a second generation developing, from 24% to 37% to 62%-99% under current and future climates, respectively. These findings demonstrate the potential for significant alterations in carrot weevil population dynamics, resulting in increased pest pressure on crops. Further research is needed to fully understand the implications of these findings and to develop effective adaptation measures to mitigate the negative impacts of global warming on insect populations and agriculture.
Topics: Animals; Weevils; Climate Change; Diapause, Insect; Female; Reproduction; Oviposition; Temperature; Larva
PubMed: 38763361
DOI: 10.1016/j.jinsphys.2024.104653 -
Journal of Chromatography. B,... Jun 2024Depending on the respective research question, LC-MS/MS based bottom-up proteomics poses challenges from the initial biological sample all the way to data evaluation....
Depending on the respective research question, LC-MS/MS based bottom-up proteomics poses challenges from the initial biological sample all the way to data evaluation. The focus of this study was to investigate the influence of sample preparation techniques and data analysis parameters on protein identification in Tribolium castaneum by applying free software proteomics platform Max Quant. Multidimensional protein extraction strategies in combination with electrophoretic or chromatographic off-line protein pre-fractionation were applied to enhance the spectrum of isolated proteins from T. castaneum and reduce the effect of co-elution and ion suppression effects during nano-LC-MS/MS measurements of peptides. For comprehensive data analysis, MaxQuant was used for protein identification and R for data evaluation. A wide range of parameters were evaluated to gain reproducible, reliable, and significant protein identifications. A simple phosphate buffer, pH 8, containing protease and phosphatase inhibitor cocktail and application of gentle extraction conditions were used as a first extraction step for T.castaneum proteins. Furthermore, a two-dimensional extraction procedure in combination with electrophoretic pre-fractionation of extracted proteins and subsequent in-gel digest resulted in almost 100% increase of identified proteins when compared to chromatographic fractionation as well as one-pot-analysis. The additionally identified proteins could be assigned to new molecular functions or cell compartments, emphasizing the positive effect of extended sample preparation in bottom-up proteomics. Besides the number of peptides during post-processing, MaxQuant's Match between Runs exhibited a crucial effect on the number of identified proteins. A maximum relative standard deviation of 2% must be considered for the data analysis. Our work with Tribolium castaneum larvae demonstrates that sometimes - depending on matrix and research question - more complex and time-consuming sample preparation can be advantageous for isolation and identification of additional proteins in bottom-up proteomics.
Topics: Animals; Proteomics; Tribolium; Tandem Mass Spectrometry; Insect Proteins; Chromatography, Liquid; Computational Biology; Proteome
PubMed: 38759531
DOI: 10.1016/j.jchromb.2024.124128