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Sensors (Basel, Switzerland) May 2023In order to advance the development of sensors fabricated with monofunctional sensation systems capable of a versatile response to tactile, thermal, gustatory,...
In order to advance the development of sensors fabricated with monofunctional sensation systems capable of a versatile response to tactile, thermal, gustatory, olfactory, and auditory sensations, mechanoreceptors fabricated as a single platform with an electric circuit require investigation. In addition, it is essential to resolve the complicated structure of the sensor. In order to realize the single platform, our proposed hybrid fluid (HF) rubber mechanoreceptors of free nerve endings, Merkel cells, Krause end bulbs, Meissner corpuscles, Ruffini endings, and Pacinian corpuscles mimicking the bio-inspired five senses are useful enough to facilitate the fabrication process for the resolution of the complicated structure. This study used electrochemical impedance spectroscopy (EIS) to elucidate the intrinsic structure of the single platform and the physical mechanisms of the firing rate such as slow adaption (SA) and fast adaption (FA), which were induced from the structure and involved the capacitance, inductance, reactance, etc. of the HF rubber mechanoreceptors. In addition, the relations among the firing rates of the various sensations were clarified. The adaption of the firing rate in the thermal sensation is the opposite of that in the tactile sensation. The firing rates in the gustation, olfaction, and auditory sensations at frequencies of less than 1 kHz have the same adaption as in the tactile sensation. The present findings are useful not only in the field of neurophysiology, to research the biochemical reactions of neurons and brain perceptions of stimuli, but also in the field of sensors, to advance salient developments in sensors mimicking bio-inspired sensations.
Topics: Smell; Rubber; Touch; Mechanoreceptors; Thermosensing
PubMed: 37430506
DOI: 10.3390/s23104593 -
BioRxiv : the Preprint Server For... Mar 2023Specialized mechanosensory end organs within mammalian skin-hair follicle-associated lanceolate complexes, Meissner corpuscles, and Pacinian corpuscles-enable our...
Specialized mechanosensory end organs within mammalian skin-hair follicle-associated lanceolate complexes, Meissner corpuscles, and Pacinian corpuscles-enable our perception of light, dynamic touch . In each of these end organs, fast-conducting mechanically sensitive neurons, called Aβ low-threshold mechanoreceptors (Aβ LTMRs), associate with resident glial cells, known as terminal Schwann cells (TSCs) or lamellar cells, to form complex axon ending structures. Lanceolate-forming and corpuscle-innervating Aβ LTMRs share a low threshold for mechanical activation, a rapidly adapting (RA) response to force indentation, and high sensitivity to dynamic stimuli . How mechanical stimuli lead to activation of the requisite mechanotransduction channel Piezo2 and Aβ RA-LTMR excitation across the morphologically dissimilar mechanosensory end organ structures is not understood. Here, we report the precise subcellular distribution of Piezo2 and high-resolution, isotropic 3D reconstructions of all three end organs formed by Aβ RA-LTMRs determined by large volume enhanced Focused Ion Beam Scanning Electron Microscopy (FIB-SEM) imaging. We found that within each end organ, Piezo2 is enriched along the sensory axon membrane and is minimally or not expressed in TSCs and lamellar cells. We also observed a large number of small cytoplasmic protrusions enriched along the Aβ RA-LTMR axon terminals associated with hair follicles, Meissner corpuscles, and Pacinian corpuscles. These axon protrusions reside within close proximity to axonal Piezo2, occasionally contain the channel, and often form adherens junctions with nearby non-neuronal cells. Our findings support a unified model for Aβ RA-LTMR activation in which axon protrusions anchor Aβ RA-LTMR axon terminals to specialized end organ cells, enabling mechanical stimuli to stretch the axon in hundreds to thousands of sites across an individual end organ and leading to activation of proximal Piezo2 channels and excitation of the neuron.
PubMed: 36993253
DOI: 10.1101/2023.03.17.533188 -
Sensors (Basel, Switzerland) Jan 2023In order to advance engineering applications of robotics such as wearable health-monitoring devices, humanoid robots, etc., it is essential to investigate the tactile...
In order to advance engineering applications of robotics such as wearable health-monitoring devices, humanoid robots, etc., it is essential to investigate the tactile sensations of artificial haptic sensors mimicking bioinspired human cutaneous mechanoreceptors such as free nerve endings, Merkel's cells, Krause end bulbs, Meissner corpuscles, Ruffini endings, and Pacinian corpuscles. The generated receptor's potential response to extraneous stimuli, categorized as slow adaption (SA) or fast adaption (FA), is particularly significant as a typical property. The present study addressed the estimation of SA and FA by utilizing morphologically fabricated mechanoreceptors made of our proposed magnetically responsive intelligent fluid, hybrid fluid (HF), and by applying our proposed electrolytic polymerization. Electric circuit models of the mechanoreceptors were generated using experimental data on capacitance and inductance on the basis of the electric characteristics of impedance. The present results regarding equivalent firing rates based on FA and SA are consistent with the FA and SA findings of vital mechanoreceptors by biomedical analysis. The present investigative process is useful to clarify the time of response to a force on the fabricated artificial mechanoreceptor.
PubMed: 36772367
DOI: 10.3390/s23031327 -
Tomography (Ann Arbor, Mich.) Sep 2022Anatomically accurate models of a human finger can be useful in simulating various disorders. In order to have potential clinical value, such models need to include a...
Anatomically accurate models of a human finger can be useful in simulating various disorders. In order to have potential clinical value, such models need to include a large number of tissue types, identified by an experienced professional, and should be versatile enough to be readily tailored to specific pathologies. Magnetic resonance images were acquired at ultrahigh magnetic field (7 T) with a radio-frequency coil specially designed for finger imaging. Segmentation was carried out under the supervision of an experienced radiologist to accurately capture various tissue types (TTs). The final segmented model of the human index finger had a spatial resolution of 0.2 mm and included 6,809,600 voxels. In total, 15 TTs were identified: subcutis, Pacinian corpuscle, nerve, vein, artery, tendon, collateral ligament, volar plate, pulley A4, bone, cartilage, synovial cavity, joint capsule, epidermis and dermis. The model was applied to the conditions of arthritic joint, ruptured tendon and variations in the geometry of a finger. High-resolution magnetic resonance images along with careful segmentation proved useful in the construction of an anatomically accurate model of the human index finger. An example illustrating the utility of the model in biomedical applications is shown. As the model includes a number of tissue types, it may present a solid foundation for future simulations of various musculoskeletal disease processes in human joints.
Topics: Humans; Magnetic Resonance Imaging; Fingers; Radio Waves; Tendons; Tendon Injuries
PubMed: 36287795
DOI: 10.3390/tomography8050196 -
Proceedings of the National Academy of... Oct 2022Low-threshold mechanoreceptors (LTMRs) and their cutaneous end organs convert light mechanical forces acting on the skin into electrical signals that propagate to the...
Low-threshold mechanoreceptors (LTMRs) and their cutaneous end organs convert light mechanical forces acting on the skin into electrical signals that propagate to the central nervous system. In mouse hairy skin, hair follicle-associated longitudinal lanceolate complexes, which are end organs comprising LTMR axonal endings that intimately associate with terminal Schwann cell (TSC) processes, mediate LTMR responses to hair deflection and skin indentation. Here, we characterized developmental steps leading to the formation of Aβ rapidly adapting (RA)-LTMR and Aδ-LTMR lanceolate complexes. During early postnatal development, Aβ RA-LTMRs and Aδ-LTMRs extend and prune cutaneous axonal branches in close association with nascent TSC processes. Netrin-G1 is expressed in these developing Aβ RA-LTMR and Aδ-LTMR lanceolate endings, and ablation experiments indicate that Netrin-G1 functions in sensory neurons to promote lanceolate ending elaboration around hair follicles. The Netrin-G ligand (NGL-1), encoded by , is expressed in TSCs, and ablation of partially phenocopied the lanceolate complex deficits observed in mutants. Moreover, NGL-1-Netrin-G1 signaling is a general mediator of LTMR end organ formation across diverse tissue types demonstrated by the fact that Aβ RA-LTMR endings associated with Meissner corpuscles and Pacinian corpuscles are also compromised in the and mutant mice. Thus, axon-glia interactions, mediated in part by NGL-1-Netrin-G1 signaling, promote LTMR end organ formation.
Topics: Animals; Mice; Axons; Ligands; Mechanoreceptors; Netrins; Schwann Cells; Skin
PubMed: 36252008
DOI: 10.1073/pnas.2210421119 -
Biomedical Research (Tokyo, Japan) 2022Peripheral nerves are provided with a blood-nerve barrier which prevents the invasion of harmful substances and pathogens, and also regulates metabolic and ionic... (Review)
Review
Peripheral nerves are provided with a blood-nerve barrier which prevents the invasion of harmful substances and pathogens, and also regulates metabolic and ionic homeostasis within nerve fascicles. The barrier functions are attributed to both the concentric layer of flattened cells in the perineurium and blood vessels running in the endoneurium. The perineurial cells develop continuous tight junctions as a diffusion barrier. In order to take up a predominant nutrient, glucose, the perineurium as well as endoneurial capillaries expresses GLUT1, a glucose transporter. An axon-Schwann cell complex within peripheral nerves utilizes glucose as a major energy source via the GLUT1, as does the brain. Under conditions of a reduced utilization of glucose, only the perineurial cells can transfer other nutrients, namely monocarboxylates such as ketone bodies and lactate via MCT1. Thus, MCT1 colocalizes with GLUT1 in the perineurium but not in endoneurial capillaries. To identify the cellular origins of the nerve sheath, marker proteins such as glial specific S100 protein, GLUT1, endoneurial CD34, and EMA (epithelial membrane antigen) are useful. Immunohistochemical findings for these markers are reviewed in this paper, focusing on the perineurium and endoneurium and their derivatives, Pacinian and Meissner corpuscles. Growing evidence throws light on the critical involvement of the nerve sheaths in the development, maintenance, and diseases of peripheral nerves.
Topics: Glucose; Glucose Transporter Type 1; Ketone Bodies; Lactates; Mucin-1; Peripheral Nerves; S100 Proteins
PubMed: 36244793
DOI: 10.2220/biomedres.43.145 -
Sensors (Basel, Switzerland) Sep 2022The development of gustatory sensors is essential for the development of smart materials for use in robotics, and in the food, beverage, and pharmaceutical industries....
The development of gustatory sensors is essential for the development of smart materials for use in robotics, and in the food, beverage, and pharmaceutical industries. We therefore designed a prototype of a rubber tongue embedded with a gustatory receptor mimicking a human tongue using our previously proposed hybrid fluid rubber (HF rubber) and an electrolytic polymerization technique. The fabricated gustatory receptor was composed of Pacinian corpuscles, which are well known and have already been elucidated as effective haptic and auditory receptors in previous studies. Moreover, the receptor has self-powered voltage generated as built-in electricity as a result of the ionized particles and molecules in the HF rubber. The utilization of a layered structure for the Pacinian corpuscles induced a typical response not only to normal and shear forces but to thermal variations. Typical gustatory characteristics, including the initial response voltage and the cyclic voltammogram form, were clearly varied by five tastes: saltiness, sourness, sweetness, bitterness, and umami. These results were due to ORP, pH, and conductivity.
Topics: Electrolytes; Humans; Polymerization; Rubber; Smart Materials; Taste; Tongue
PubMed: 36146328
DOI: 10.3390/s22186979 -
Sensors (Basel, Switzerland) Jul 2022The development of auditory sensors and systems is essential in smart materials of robotics and is placed at the strategic category of mutual communication between...
The development of auditory sensors and systems is essential in smart materials of robotics and is placed at the strategic category of mutual communication between humans and robots. We designed prototypes of the rubber-made equilibrium and auditory sensors, mimicking hair cells in the saccule and the cochlea at the vestibule of the human ear by utilizing our previously proposed technique of electrolytic polymerization on the hybrid fluid rubber (HF rubber). The fabricated artificial hair cells embedded with mimicked free nerve endings and Pacinian corpuscles, which are well-known receptors in the human skin and have already been elucidated effective in the previous study, have the intelligence of equilibrium and auditory sensing. Moreover, they have a voltage that is generated from built-in electricity caused by the ionized particles and molecules in the HF rubber due to piezoelectricity. We verified the equilibrium and auditory characteristics by measuring the changes in voltage with inclination, vibration over a wide frequency range, and sound waves. We elucidated experimentally that the intelligence has optimum morphological conditions. This work has the possibility of advancing the novel technology of state-of-the-art social robotics.
Topics: Humans; Polymerization; Robotics; Rubber; Skin, Artificial; Smart Materials
PubMed: 35891135
DOI: 10.3390/s22145447