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Antimicrobial Agents and Chemotherapy Oct 1987We examined 741 urinary tract isolates of the Morganella-Proteus-Providencia group, including the recently defined species Proteus penneri, for susceptibilities to... (Comparative Study)
Comparative Study
We examined 741 urinary tract isolates of the Morganella-Proteus-Providencia group, including the recently defined species Proteus penneri, for susceptibilities to aminoglycosides, semisynthetic penicillins, and cephalosporins. The data emphasize the importance of identification to the species level on the basis of marked species differences in patterns of susceptibility.
Topics: Anti-Bacterial Agents; Drug Resistance, Microbial; Enterobacteriaceae; Humans; Microbial Sensitivity Tests; Proteus; Providencia; Urinary Tract Infections
PubMed: 3435110
DOI: 10.1128/AAC.31.10.1644 -
Journal of Clinical Microbiology Jun 1987Twenty strains of Proteus penneri obtained from the Centers for Disease Control, Atlanta, Ga., were tested for their ability to hemolyze sheep and human erythrocytes, a...
Twenty strains of Proteus penneri obtained from the Centers for Disease Control, Atlanta, Ga., were tested for their ability to hemolyze sheep and human erythrocytes, a property that is thought to be connected with the invasiveness and virulence of Proteus species. In the logarithmic phase of growth, P. penneri cultures are hemolytic for such erythrocytes. This ability is comparable to the hemolysis exhibited by nearly 100% of P. vulgaris and P. mirabilis strains, which is due to the production of a cell-bound hemolytic factor; is demonstrated only in broth cultures; and seems to facilitate the penetration of P. penneri and other Proteus species into the cells without cytotoxic effects. In contrast, a filterable alpha-hemolysin, which is produced transiently by a very few strains of P. mirabilis, was present in 4 of 20 P. penneri strains. This property, which is expressed at a high level over a long period, suggests a chromosomal origin. The penetration of an alpha-hemolytic P. penneri strain into Vero cells was accompanied by a drastic cytotoxic effect.
Topics: Animals; Culture Media; Hemolysin Proteins; Hemolysis; Humans; Proteus; Sheep; Vero Cells; Virulence
PubMed: 3597752
DOI: 10.1128/jcm.25.6.1094-1096.1987 -
Journal of Clinical Microbiology Mar 1987Strains of Proteus penneri from seven abdominal wounds (after bowel resection), five urine samples, and eight other sites were isolated in mixed cultures. Seven urine...
Strains of Proteus penneri from seven abdominal wounds (after bowel resection), five urine samples, and eight other sites were isolated in mixed cultures. Seven urine isolates were in pure cultures. All infections were nosocomially acquired, indicating that complete identification of P. penneri in the clinical laboratory is warranted.
Topics: Aged; Aged, 80 and over; Bacteriuria; Child, Preschool; Cross Infection; Female; Humans; Male; Middle Aged; Proteus; Proteus Infections
PubMed: 3571463
DOI: 10.1128/jcm.25.3.578-579.1987 -
The Journal of Antibiotics Jul 1986A cephalosporin-hydrolyzing enzyme from strains of Proteus penneri resistant to beta-lactam antibiotics was purified and characterized. The enzyme gave a single protein...
A cephalosporin-hydrolyzing enzyme from strains of Proteus penneri resistant to beta-lactam antibiotics was purified and characterized. The enzyme gave a single protein band on SDS-polyacrylamide gel electrophoresis with a molecular weight of 30,000. This cephalosporinase has an isoelectric point of 6.8, a pH optimum of 6.5 and a temperature optimum of 45 degrees C. The enzyme hydrolyzed cephaloridine, cephalothin, cefuroxime, and cefotaxime more rapidly than penicillins. The relative rate, with cephaloridine as 100, were: cephalothin, 50; cefuroxime, 93; cefotaxime, 48; ceftriaxone, 23; cefoperazone, 11; benzylpenicillin, 3; ampicillin, 9; and carbenicillin, less than 1. Cephamycins had low affinities for the enzyme. However, clavulanic acid and sulbactam, with high affinities for the enzyme, were inhibitors of this enzyme.
Topics: Anti-Bacterial Agents; Molecular Weight; Proteus; beta-Lactamases; beta-Lactams
PubMed: 3489701
DOI: 10.7164/antibiotics.39.938 -
Journal of Clinical Microbiology Feb 1986A total of 2,693 fecal specimens, with 1,422 from healthy persons and 1,271 from patients suffering from enteric diseases, was investigated to isolate species of the...
A total of 2,693 fecal specimens, with 1,422 from healthy persons and 1,271 from patients suffering from enteric diseases, was investigated to isolate species of the Morganella-Proteus-Providencia group and to evaluate the role of these bacteria in intestinal disorders. Most strains were isolated from two media, i.e., blood agar and tryptophan agar. Two of the species were more frequently found in diarrheal cases than in healthy controls. These species were Morganella morganii and Proteus mirabilis. Two new species of Enterobacteriaceae, i.e., Proteus penneri and Providencia rustigianii, were found in 33 and 5 people, respectively. However, these two species were not found more frequently in the diarrheal cases.
Topics: Culture Media; Diarrhea; Enterobacteriaceae; Enterobacteriaceae Infections; Feces; Germany, West; Humans; Intestinal Diseases; Proteus; Proteus Infections; Proteus mirabilis; Providencia
PubMed: 3517057
DOI: 10.1128/jcm.23.2.404-405.1986 -
Antimicrobial Agents and Chemotherapy Sep 1984Patterns of susceptibility of 45 Proteus penneri clinical isolates to 14 antimicrobial agents were evaluated by a macrobroth dilution method. All strains were highly...
Patterns of susceptibility of 45 Proteus penneri clinical isolates to 14 antimicrobial agents were evaluated by a macrobroth dilution method. All strains were highly susceptible to ceftizoxime, ceftazidime, moxalactam, cefoxitin, gentamicin, tobramycin, netilmicin, and, with few exceptions, to amikacin, piperacillin, and cefoperazone. Most strains were susceptible to cefotaxime and ceftriaxone. All strains were resistant to cefazolin and cefsulodin.
Topics: Anti-Bacterial Agents; Microbial Sensitivity Tests; Proteus
PubMed: 6508270
DOI: 10.1128/AAC.26.3.419 -
Journal of Clinical Microbiology Apr 1984The clinical significance of Proteus penneri, a newly described species, is unknown. A case report is presented, which is to the best of our knowledge the first...
The clinical significance of Proteus penneri, a newly described species, is unknown. A case report is presented, which is to the best of our knowledge the first description of this organism causing a urinary tract infection and bladder calculi.
Topics: Aged; Hematuria; Humans; Male; Proteus; Proteus Infections; Urinary Bladder Calculi; Urinary Tract Infections
PubMed: 6715521
DOI: 10.1128/jcm.19.4.541-542.1984 -
Journal of Clinical Microbiology Jun 1982The name Proteus penneri sp. nov. is proposed for a group of organisms previously called Proteus vulgaris indole negative or P. vulgaris biogroup 1. All of these strains...
The name Proteus penneri sp. nov. is proposed for a group of organisms previously called Proteus vulgaris indole negative or P. vulgaris biogroup 1. All of these strains were salicin negative, esculin negative, and chloramphenicol resistant (zone size, less than 14 mm). DNA relatedness studies indicated that when DNA from P. penneri strain 1808-73 was labeled and tested against unlabeled DNA from 13 other P penneri strains, a highly related group was formed (88 to 99% relatedness at 60 degrees C and 67 to 99% relatedness at 75 degrees C). Strain 1808-73 (ATCC 33519) is proposed as the type strain of P. penneri. In this study, two distinct groups of indole-positive P. vulgaris strains were also apparent. The first group (defined as P. vulgaris biogroup 2) was indole positive, salicin positive, and esculin positive, and the second group (defined as P. vulgaris biogroup 3) was indole positive, salicin negative, and esculin negative. The current type strain of P. vulgaris (ATCC 13315) belongs to biogroup 3. The DNA from P. penneri strains was not highly related to labeled DNA from the type strain of P. vulgaris (14 to 30% relatedness at 75 degrees C) or from P. vulgaris strain PR 1 (ATCC 29905), which belongs to biogroup 2 (27 to 33% relatedness at 75 degrees C). Strains of biogroup 2 were sensitive to chloramphenicol (zone size, greater than 19mm), and 10 of these strains formed a highly related group by DNA hybridization when DNA from PR 1 was labeled (64 to 100% relatedness at 60 degrees C and 70 to 100% relatedness at 75 degrees C), but they were not highly relatedness to the type strain of P. vulgaris (51 to 68% relatedness at 60 degrees C and 14 to 44% relatedness at 75 degrees C). Further DNA relatedness studies are needed on strains of biogroup 3 before a definitive taxonomic proposal can be made for these two indole-positive biogroups.
Topics: Anti-Bacterial Agents; Base Composition; Cytosine; DNA, Bacterial; Guanine; Indoles; Nucleic Acid Hybridization; Proteus; Proteus vulgaris; Terminology as Topic
PubMed: 7050147
DOI: 10.1128/jcm.15.6.1097-1102.1982