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Medicine Jan 2022Infections with Sphingomonas paucimobilis are rarely described in the literature and can be community-acquired or associated with healthcare, especially in patients with...
RATIONALE
Infections with Sphingomonas paucimobilis are rarely described in the literature and can be community-acquired or associated with healthcare, especially in patients with chronic conditions (e.g., diabetes mellitus), malignancies, or other causes of immunosuppression, except in people without comorbidities. We present the case of a patient with diabetes mellitus and hypertension diagnosed during a routine evaluation, with splenic abscess caused by S paucimobilis. Our literature search revealed no other case report of splenic abscess caused only by S paucimobilis.
PATIENT CONCERNS
We present the case of a 55-year-old Caucasian man with type 2 diabetes mellitus and hypertension.
DIAGNOSIS
Thoraco-abdominal computed tomography revealed splenomegaly of 20X16X18 cm, with a homogeneous subcapsular hypodense collection, with a mass effect on the left hemidiaphragm.
INTERVENTIONS
The patient underwent surgical intervention and S paucimobilis was isolated on blood agar.
OUTCOME
The patient received treatment with ciprofloxacin (500 mg twice daily) for 14 days, with favorable outcomes.
LESSONS
S paucimobilis, a low-virulence bacterium, can cause community-acquired or nosocomial infections. Visceral localizations, usually symptomatic, can evolve rapidly, and the diagnosis is associated with complications or, as in our case, with careful investigation of some changes in laboratory investigations.
Topics: Abdominal Abscess; Diabetes Mellitus, Type 2; Gram-Negative Bacterial Infections; Humans; Hypertension; Intraabdominal Infections; Male; Middle Aged; Sphingomonas; Splenic Diseases; Splenomegaly; Tomography, X-Ray Computed
PubMed: 35029916
DOI: 10.1097/MD.0000000000028522 -
Microbiology Spectrum Feb 2022In an ongoing microbial tracking investigation of the International Space Station (ISS), several strains were isolated. Based on the 16S rRNA gene sequence,...
In an ongoing microbial tracking investigation of the International Space Station (ISS), several strains were isolated. Based on the 16S rRNA gene sequence, phylogenetic analysis identified the ISS strains as Sphingomonas sanguinis ( = 2) and one strain isolated from the Kennedy Space Center cleanroom (used to assemble various Mars mission spacecraft components) as Sphingomonas paucimobilis. Metagenomic sequence analyses of different ISS locations identified 23 species. An abundance of shotgun metagenomic reads were detected for S. sanguinis in the location from where the ISS strains were isolated. A complete metagenome-assembled genome was generated from the shotgun reads metagenome, and its comparison with the whole-genome sequences (WGS) of the ISS S. sanguinis isolates revealed that they were highly similar. In addition to the phylogeny, the WGS of these strains were compared with the WGS of the type strains to elucidate genes that can potentially aid in plant growth promotion. Furthermore, the WGS comparison of these strains with the well-characterized sp. LK11, an arid desert strain, identified several genes responsible for the production of phytohormones and for stress tolerance. Production of one of the phytohormones, indole-3-acetic acid, was further confirmed in the ISS strains using liquid chromatography-mass spectrometry. Pathways associated with phosphate uptake, metabolism, and solubilization in soil were conserved across all the S. sanguinis and S. paucimobilis strains tested. Furthermore, genes thought to promote plant resistance to abiotic stress, including heat/cold shock response, heavy metal resistance, and oxidative and osmotic stress resistance, appear to be present in these space-related S. sanguinis and strains. Characterizing these biotechnologically important microorganisms found on the ISS and harnessing their key features will aid in the development of self-sustainable long-term space missions in the future. is ubiquitous in nature, including the anthropogenically contaminated extreme environments. Members of the genus have been identified as potential candidates for space biomining beyond earth. This study describes the isolation and identification of members from the ISS, which are capable of producing the phytohormone indole-3-acetic acid. Microbial production of phytohormones will help future studies, grow plants beyond low earth orbit, and establish self-sustainable life support systems. Beyond phytohormone production, stable genomic elements of abiotic stress resistance, heavy metal resistance, and oxidative and osmotic stress resistance were identified, rendering the ISS isolate a strong candidate for biotechnology-related applications.
Topics: Genomics; Indoleacetic Acids; Metagenome; Metagenomics; Phylogeny; Plant Development; Plant Growth Regulators; RNA, Ribosomal, 16S; Spacecraft; Sphingomonas; Whole Genome Sequencing
PubMed: 35019675
DOI: 10.1128/spectrum.01994-21 -
Iranian Journal of Microbiology Oct 2021is an opportunistic pathogen and was rarely encountered in clinical specimens previously. This study aimed to investigate the clinical features, associated...
BACKGROUND AND OBJECTIVES
is an opportunistic pathogen and was rarely encountered in clinical specimens previously. This study aimed to investigate the clinical features, associated co-morbidities, and antimicrobial susceptibility patterns of infection in a tertiary hospital in Uttarakhand.
MATERIALS AND METHODS
isolates cultured from various sections of hospital and OPDs were identified and analyzed for their antibiograms in the microbiology laboratory for a duration of one year from January 2020 to December 2020.
RESULTS
was isolated from 49 samples (0.01%) out of 3792 samples processed in VITEK 2 Compact automated ID/AST instrument. The maximum number of isolates were obtained from urine samples (31%), followed by blood (24%). Septicemia (41%), meningitis (17%), lower respiratory tract infections and ventilator associated pneumonia (14%) constituted a major portion of infections caused by this organism. Diabetes mellitus (22%) and steroid usage (16%) were major associated co-morbid conditions. Third and Fourth generation cephalosporins like ceftriaxone (81%) and cefepime (86%) were found to be the most susceptible drugs whereas 61% of isolates were resistant to colistin.
CONCLUSION
This organism is an up-and-coming pathogen and should not be simply labeled as a contaminant. Although the organism is not grossly virulent and still might not be associated with serious life-threatening infections; however their evolving resistance patterns and increased spectrum of infections should be seriously taken into account.
PubMed: 34900159
DOI: 10.18502/ijm.v13i5.7425 -
SAGE Open Nursing 2021Knowledge management-based nursing care has a positive effect in preventing healthcare associated infections (HAIs). Therefore, nursing professionals can utilize key...
INTRODUCTION
Knowledge management-based nursing care has a positive effect in preventing healthcare associated infections (HAIs). Therefore, nursing professionals can utilize key strategies of knowledge management to support clinical decision making, reorganize nursing actions, and maximize patient outcomes.
OBJECTIVES
The aim of this study was to determine the effect of knowledge management-based nursing care educational training on HAI prevention behavior at the High Care Unit (HCU) of Saiful Anwar Hospital Malang.
METHODS
A quasiexperimental design with a pretest, educational training intervention, and posttest were conducted on 15 nurses in the HCU of Saiful Anwar Hospital Malang, which lasted for 16 days. Furthermore, observation of nursing care documentation, nurses' handwashing compliance, and presence of infection-causing bacteria in the HCU staff and environment (hands rub handle, medical record, and patient's bed) was carried out pre (day 1-7) and post training (day 10-16). Subsequently, educational training related to knowledge management-based nursing care was conducted for 2 days (day 8-9) by the Doktor Mengabdi Team of Universitas Brawijaya.
RESULTS
The knowledge level and completeness of the nursing care documentation in the HCU room significantly increased after the training (< .05). Also, compliance to the six steps five moments of nurses' handwashing increased after the training (> .05). Infection-causing bacteria were found in the HCU environment and staff before and after the training involving , , , , , , and . Therefore, increased knowledge of HCU nurses and complete documentation ( = .890; = .054), increased knowledge of HCU nurses and handwashing compliance ( = .770; = .086), and handwashing compliance and bacterial presence ( = .816; = .084) all had a positive correlation.
CONCLUSION
Knowledge management-based nursing care educational training increased infection prevention behavior in the HCU of Saiful Anwar Hospital Malang.
PubMed: 34869859
DOI: 10.1177/23779608211044601 -
Revista Brasileira de Ginecologia E... Oct 2021Breast surgery is considered a clean surgery; however, the rates of infection range between 3 and 15%. The objective of the present study was to intraoperatively...
OBJECTIVE
Breast surgery is considered a clean surgery; however, the rates of infection range between 3 and 15%. The objective of the present study was to intraoperatively investigate the presence of autochthonous microbiota in the breast.
METHODS
Pieces of breast tissue collected from 49 patients who underwent elective breast surgery (reconstructive, diagnostic, or oncologic) were cultured. The pieces of breast tissue were approximately 1 cm in diameter and were removed from the retroareolar area, medial quadrant, and lateral quadrant. Each piece of tissue was incubated in brain heart infusion (BHI) broth for 7 days at 37°C, and in cases in which the medium became turbid due to microorganism growth, the samples were placed in Petri dishes for culturing and isolating strains and for identifying species using an automated counter.
RESULTS
Microorganism growth was observed in the samples of 10 of the 49 patients (20.4%) and in 11 of the 218 pieces of tissue (5%). The detected species were , and No patient with positive samples had clinical infection postoperatively.
CONCLUSION
The presence of these bacteria in breast tissue in approximately 20% of the patients in this series suggests that breast surgery should be considered a potential source of contamination that may have implications for adverse reactions to breast implants and should be studied in the near future for their oncological implications in breast implant-associated large-cell lymphoma etiology.
Topics: Bacteria; Breast; Breast Implants; Humans; Microbiota
PubMed: 34784632
DOI: 10.1055/s-0041-1736300 -
Anais Da Academia Brasileira de Ciencias 2021Biosynthesis of titanium dioxide nanoparticles (TiO2NPs) by Sphingomonas paucimobilis B34 bacteria was successfully achieved and followed by UV-Vis spectroscopy. The...
Biosynthesis of titanium dioxide nanoparticles (TiO2NPs) by Sphingomonas paucimobilis B34 bacteria was successfully achieved and followed by UV-Vis spectroscopy. The nanoparticles were characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM) and fourier transform infrared spectroscopy (FTIR) techniques. The biosynthesized TiO2NPs were spherical in shape with an average particle size of 15.6 nm. These TiO2NPs were used as nono-catalyst for removing of malachite green (MG) dye (at 103 mol/L) from wastewater solution. As indicated by the results, the biosynthesized TiO2NPs represented a capable approach for MG removal with up to 83 % efficiency. The removal process was found to follow a pseudo-first-order kinetics. Furthermore, the developed TiO2NPs-MG hybrid nanocomposite was efficiently removed from the medium by using Spirulina platensis cyanobacterial biomass after wastewater treatment. S. platensis biomass was able to remove up to 89.43 % of the hybrid nanocomposite by a biosorption process. The resultant water effluent, after TiO2NPs-MG removal, showed no toxicity towards Vigna radiate L. seedlings implying its safety for agriculture purposes. According to the obtained results, S. platensis living biomass could play a dual re-cycling role, as natural biosorbent for removing both nanoparticles and dye (TiO2NPs-MG hybrid nano-composite) from solution after wastewater treatment for healthier environmental management.
Topics: Biomass; Nanocomposites; Nanoparticles; Rosaniline Dyes; Sphingomonas; Spirulina; Titanium; Water Purification
PubMed: 34730741
DOI: 10.1590/0001-3765202120201669 -
Children (Basel, Switzerland) Oct 2021Pediatric patients are more susceptible and vulnerable to nosocomial infections, in part because of their nascent and developing immune system and in part due to certain...
Pediatric patients are more susceptible and vulnerable to nosocomial infections, in part because of their nascent and developing immune system and in part due to certain congenital conditions. Consequently, we found limited literature that investigated and reported children's toys in hospital playrooms as potential reservoirs of pathogenic microbes. Hence, in the present study, we aimed to investigate toys as potential vectors for nosocomial infections in children's hospitals. Microbiological samples from 120 toys were collected between April 2018 and November 2018. The specimens were cultivated on suitable cultivation agars for 24-72 h at 37 °C and CFU/cm (colony forming units) was determined. Antibiotic susceptibility testing was performed using disc diffusion and E-tests. Our results indicate that 84% of samples were contaminated with different microbes. Four distinct genera and thirty-seven species of bacteria were identified. The most frequently isolated pathogen was (>603 CFU/cm). Most of the identified microorganisms were members of normal human microbiota. Although and were identified, CFU/cm was relatively low and they were found to be sensitive to antibiotics. Additionally, plastic toys showed the highest average CFU/cm of 91.9. Our results bolster the need for adoption and strict enforcement of proper disinfection techniques for toys in the hospital playrooms.
PubMed: 34682179
DOI: 10.3390/children8100914 -
Saudi Journal of Ophthalmology :... 2021With increased availability of sophisticated microbiological techniques for isolation, growth and identification of micro-organisms the spectrum of organisms is rapidly....
With increased availability of sophisticated microbiological techniques for isolation, growth and identification of micro-organisms the spectrum of organisms is rapidly. Herein we report four cases of canaliculitis with unusual organisms and highlight their clinical significance. To the best of our knowledge, there are no reports of isolation of Brucella melitensis and Leuconostoc species reported in English literature; and only one report of isolation of Myroides species from canaliculitis exists. Sphingomonas paucimobilis, is an uncommon isolate in canaliculitis. Extremes of age and occupational exposure may be possible risk factors for infection with uncommon organisms. Clinical features at presentation do not vary greatly with uncommon or multi drug resistant organisms' hence sampling and microbiological assessment is warranted. The benefit of curettage in canaliculitis is manifold. Unusual organisms and opportunistic pathogens can be multi-drug resistant and determination of antibiotic susceptibility is important to initiate targeted therapy to ensure disease cure and prevent recurrences.
PubMed: 34667936
DOI: 10.4103/1319-4534.325778 -
Journal of Applied Glycoscience 2020α-L-Rhamnosidases (α-L-Rha-ases, EC 3.2.1.40) are glycosyl hydrolases (GHs) that hydrolyze a terminal α-linked L-rhamnose residue from a wide spectrum of substrates...
α-L-Rhamnosidases (α-L-Rha-ases, EC 3.2.1.40) are glycosyl hydrolases (GHs) that hydrolyze a terminal α-linked L-rhamnose residue from a wide spectrum of substrates such as heteropolysaccharides, glycosylated proteins, and natural flavonoids. As a result, they are considered catalysts of interest for various biotechnological applications. α-L-rhamnose (6-deoxy-L-mannose) is structurally similar to the rare sugar α-L-mannose. Here we have examined whether microbial α-L-Rha-ases possess α-L-mannosidase activity by synthesizing the substrate 4-nitrophenyl α-L-mannopyranoside. Four α-L-Rha-ases from GH78 and GH106 families were expressed and purified from cells. All four enzymes exhibited both α-L-rhamnosyl-hydrolyzing activity and weak α-L-mannosyl-hydrolyzing activity. SpRhaM, a GH106 family α-L-Rha-ase from FP2001, was found to have relatively higher α-L-mannosidase activity as compared with three GH78 α-L-Rha-ases. The α-L-mannosidase activity of SpRhaM showed pH dependence, with highest activity observed at pH 7.0. In summary, we have shown that α-L-Rha-ases also have α-L-mannosidase activity. Our findings will be useful in the identification and structural determination of α-L-mannose-containing polysaccharides from natural sources for use in the pharmaceutical and food industries.
PubMed: 34354534
DOI: 10.5458/jag.jag.JAG-2020_0005 -
Journal of Orthopaedic Case Reports Apr 2021Sphingomonas paucimobilis is an opportunistic pathogen and a rare cause of human infection. This case report shows bacteremia with pyogenic spondylodiscitis in...
INTRODUCTION
Sphingomonas paucimobilis is an opportunistic pathogen and a rare cause of human infection. This case report shows bacteremia with pyogenic spondylodiscitis in lumbosacral spine caused by Sphingomonas and discusses its clinical diagnosis, treatment, and literature reviews.
CASE REPORT
Patient presented with severe low back pain, inability to walk and fever following a L5-S1 decompression elsewhere, which corresponded clinico-radiologically with a picture of a spondylodiscitis on radiographs, magnetic resonance imaging, and computed tomography. His blood culture was positive for S. paucimobilis. He was treated successfully by surgical debridement, stabilization, and fusion along with intravenous Linezolid followed by oral therapy, based on the antibiotic susceptibility profile. Repeat blood culture was negative after 7 weeks of antibiotic therapy. Patient improved symptomatically with radiographs showing good fusion at 1 year follow-up. S. paucimobilis, though a low virulence organism, is an emerging pathogen and should be dealt with cautiously.
CONCLUSION
This reiterates the importance of culture as unusual organisms may be isolated and appropriate antibiotics form the mainstay of treatment.
PubMed: 34327174
DOI: 10.13107/jocr.2021.v11.i04.2166