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Journal of Yeungnam Medical Science Jan 2022This report describes a case of coinfection of Sphingomonas paucimobilis meningitis and Listeria monocytogenes bacteremia in a 66-year-old immunocompetent female...
This report describes a case of coinfection of Sphingomonas paucimobilis meningitis and Listeria monocytogenes bacteremia in a 66-year-old immunocompetent female patient. The patient had undergone traditional procedures, including acupuncture, which possibly caused the coinfection. During treatment with susceptible antibiotics for bacterial meningitis, she developed hydrocephalus on the third day. Consequently, the patient recovered with a mild neurological deficit of grade 4 motor assessment in both upper and lower extremities at discharge. S. paucimobilis and L. monocytogenes are rare pathogens in developed countries, occurring only during environmental outbreaks. S. paucimobilis meningitis is rarely reported. Hence, the various presentations of S. paucimobilis meningitis and the antibiotic regimen for its treatment are hereby reported, in addition to a review of other similar reported cases. This case is a possible traditional procedure-related infection. Appropriate oversight and training should be emphasized regarding preventive measures of this kind of infection. A team approach with neurologists and neurosurgeons is imperative in treating patients with hydrocephalus-complicated meningitis.
PubMed: 34092053
DOI: 10.12701/yujm.2021.01074 -
IDCases 2021() is a low-pathogenicity, gram-negative bacilli (GNB) that are previously known as an opportunist microorganism. Recent studies have shown that is an emerging...
() is a low-pathogenicity, gram-negative bacilli (GNB) that are previously known as an opportunist microorganism. Recent studies have shown that is an emerging pathogen causing various infections. Multidrug-resistant GNB has emerged as a major clinical and therapeutic dilemma in various hospital-associated infections. Although rare, could be associated with infective endocarditis (IE). Prosthetic valve endocarditis (PVE) is the most severe type of IE, which has high mortality rates despite diagnostic and treatment advances. We report a fatal case of early PVE associated with multidrug-resistant (MDR) - complicated with perivalvular abscess, complete heart block, valve detachment, and septic arthritis.
PubMed: 34026540
DOI: 10.1016/j.idcr.2021.e01152 -
Microorganisms Apr 2021An aerobic, yellow-pigmented, bacteriochlorophyll -producing strain, designated AAP5 (=DSM 111157=CCUG 74776), was isolated from the alpine lake Gossenköllesee located...
An aerobic, yellow-pigmented, bacteriochlorophyll -producing strain, designated AAP5 (=DSM 111157=CCUG 74776), was isolated from the alpine lake Gossenköllesee located in the Tyrolean Alps, Austria. Here, we report its description and polyphasic characterization. Phylogenetic analysis of the 16S rRNA gene showed that strain AAP5 belongs to the bacterial genus and has the highest pairwise 16S rRNA gene sequence similarity with (98.3%), (96.8%), and (96.5%). Its genomic DNA G + C content is 65.9%. Further, in silico DNA-DNA hybridization and calculation of the average nucleotide identity speaks for the close phylogenetic relationship of AAP5 and . The high percentage (76.2%) of shared orthologous gene clusters between strain AAP5 and NCTC 11030, the type species of the genus, supports the classification of the two strains into the same genus. Strain AAP5 was found to contain C7 (64.6%) as a predominant fatty acid (>10%) and the polar lipid profile contained phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, sphingoglycolipid, six unidentified glycolipids, one unidentified phospholipid, and two unidentified lipids. The main respiratory quinone was ubiquinone-10. Strain AAP5 is a facultative photoheterotroph containing type-2 photosynthetic reaction centers and, in addition, contains a xathorhodopsin gene. No CO-fixation pathways were found.
PubMed: 33917603
DOI: 10.3390/microorganisms9040768 -
Microbiology Resource Announcements Feb 2021Because of its small size, Gram-negative can pose a risk of nosocomial infection. We report the complete circular genome sequence of strain Kira, which was isolated...
Because of its small size, Gram-negative can pose a risk of nosocomial infection. We report the complete circular genome sequence of strain Kira, which was isolated from retinoic acid-supplemented SH-SY5Y human cell cultures, to be 3,917,410 bp (G+C content, 65.7%; 3,672 protein-coding sequences), with two plasmids (79,575 bp and 44,333 bp).
PubMed: 33574107
DOI: 10.1128/MRA.01156-20 -
Case Reports in Infectious Diseases 2020A 47-year-old male with no significant medical history was hospitalized for bacteremia and diagnosed with endocarditis. The organism isolated was a Gram-negative...
A 47-year-old male with no significant medical history was hospitalized for bacteremia and diagnosed with endocarditis. The organism isolated was a Gram-negative bacillus-. There are only a few reported cases of endocarditis caused by , and to our knowledge, this is the first in the United States.
PubMed: 33101743
DOI: 10.1155/2020/7185834 -
Journal of Biomolecular Techniques : JBT Oct 2020Microbial monitoring on the International Space Station (ISS) is essential to keep astronauts healthy. Current practice involves culture-based methods, but future...
Microbial monitoring on the International Space Station (ISS) is essential to keep astronauts healthy. Current practice involves culture-based methods, but future directives by the National Aeronautics and Space Administration (NASA) will require the use of molecular-based approaches, such as quantitative PCR (qPCR). However, in order to successfully and reliably detect the allowable limit of 5 × 10 colony forming units (CFUs) of bacteria per liter in potable water on the ISS with qPCR, water concentration must first be performed. This report presents the data from a validation study of a NASA-sponsored small business research initiative to develop a microgravity-compatible, automated water concentrator to be used on the ISS, which has been named the ISS Smart Sample Concentrator (iSSC). Efficiency and reproducibility of the iSSC were compared with a ground-based automated water concentrator and the standard Millipore manual filtration. Using 10 CFU/L of , , and and a mixed microbial community, we have shown, through culture and qPCR, that the iSSC is comparable, if not better, at recovering and concentrating bacteria from large volumes of water, with good reproducibility.
PubMed: 33100920
DOI: 10.7171/jbt.20-3104-005 -
Journal of Conservative Dentistry : JCD 2019Plant extracts are used in folklore medicine from time immemorial to treat different oral diseases. Chemical constituents extracted from these natural resources are...
CONTEXT
Plant extracts are used in folklore medicine from time immemorial to treat different oral diseases. Chemical constituents extracted from these natural resources are gifted with huge opportunities.
AIM
The aim of this study is to assess the antibacterial property of (Neem), (Tulsi), and (Pochotia) against oral microorganisms.
MATERIALS AND METHODS
Plant extract was prepared with hot continuous extraction method by the Soxhlet Apparatus. Microorganisms isolated from the oral cavity and identified by Vitek-2. Bacterial inoculums poured and spread into Mueller Hinton plates. Plant extract was poured into prepared wells taking ciprofloxacillin as the positive control and dimethyl sulfoxide as the negative control. The experiment was performed in duplicates with two different concentrations of the extract and mean value of inhibition zone was calculated.
STATISTICAL ANALYSIS
Paired -test, analysis of variance, and regression analysis.
RESULTS
Isolated microorganisms were , and . Higher zone of inhibition was observed against by followed by . Among the aqueous and acetone group, in the aqueous group, the regression models of and have been found to be statistically significant (= < 0.05), whereas, in the acetone group, the regression model of has been found to be statistically significant ( = < 0.05).
CONCLUSION
All the three plants showed antibacterial potency against the isolated organisms. Acetone group showed better efficacy than the aqueous extract group.
PubMed: 33088074
DOI: 10.4103/JCD.JCD_268_19 -
Indian Journal of Nephrology 2020is an aerobic gram-negative bacillus, widely distributed in the water and soil. It has also been found in nosocomial environments causing nosocomial infections. is a...
is an aerobic gram-negative bacillus, widely distributed in the water and soil. It has also been found in nosocomial environments causing nosocomial infections. is a rare cause of peritoneal dialysis (PD)-related peritonitis. Here, we report the 14 case, with a literature review. Our case is unique as this is the first reported case of intra-abdominal abscess associated with PD-related peritonitis.
PubMed: 33013071
DOI: 10.4103/ijn.IJN_271_19 -
New Microbes and New Infections Sep 2020Multiple studies have established the contamination of hospital sinks and transmission to hospital personnel. Few studies have assessed the contamination and...
Multiple studies have established the contamination of hospital sinks and transmission to hospital personnel. Few studies have assessed the contamination and transmission of microorganisms from the faucets of operating bay scrub sinks to operating room (OR) personnel, a potential route of infection for patients. This study aimed to investigate if there was pathogenic contamination of scrub sinks and possible transmission of those pathogens to the hands of OR personnel after preoperative hand disinfection. Swabs were taken from the hands of 50 OR personnel and from the faucets of 24 scrubs sinks at two different hospital sites, and were cultured. Hands were swabbed after completing a surgical hand scrub. Results were reported in colony-forming units per millilitre. There was significant scrub sink contamination with primarily Gram-negative organisms, such as and . There was no overlap in bacterial species between the cultures from hands and scrub sinks. Cultures from the sinks and the hands of the OR personnel from one site had significantly higher bacterial growth compared with the other site (p < 0.0001 and p < 0.0118, respectively). The data showed significant contamination on the faucets of operating bay scrub sinks. However, there was no observed transmission of pathogens from the scrub sinks to OR personnel, shown by the lack of overlap in bacterial species. Routine hygienic maintenance of scrub sinks is recommended.
PubMed: 32995014
DOI: 10.1016/j.nmni.2020.100754 -
BMC Infectious Diseases Sep 2020Vogesella species are common aquatic, Gram-negative rod-shaped bacteria, originally described in 1997. Vogesella perlucida was first isolated from spring water in 2008....
BACKGROUND
Vogesella species are common aquatic, Gram-negative rod-shaped bacteria, originally described in 1997. Vogesella perlucida was first isolated from spring water in 2008. Furthermore, bacterial pathogenicity of Vogesella perlucida has never been reported. Here, we report the first case of rare Vogesella perlucida-induced bacteremia in an advanced-age patient with many basic diseases and history of dexamethasone abuse.
CASE PRESENTATION
A 71-year-old female was admitted with inflamed upper and lower limbs, rubefaction, pain and fever (about 40 °C). She had been injured in a fall at a vegetable market and then touched river snails with her injury hands. A few days later, soft tissue infection of the patient developed and worsened. Non-pigmented colonies were isolated from blood cultures of the patient. Initially, Vogesella perlucida was wrongly identified as Sphingomonas paucimobilis by Vitek-2 system with GN card. Besides, we failed to obtain an acceptable identification by the MALDI-TOF analysis. Finally, the isolated strain was identified as Vogesella perlucida by 16S rRNA gene sequences. In addition, the patient recovered well after a continuous treatment of levofloxacin for 12 days.
CONCLUSION
Traditional microbiological testing system may be inadequate in the diagnosis of rare pathogenic bacteria. Applications of molecular diagnostics techniques have great advantages in clinical microbiology laboratory. By using 16S rRNA gene sequence analysis, we report the the first case of rare Vogesella perlucida-induced bacteremia.
Topics: Aged; Anti-Bacterial Agents; Bacteremia; Bacterial Typing Techniques; Betaproteobacteria; Female; Humans; Levofloxacin; RNA, Ribosomal, 16S; Soft Tissue Infections; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Vancomycin
PubMed: 32948147
DOI: 10.1186/s12879-020-05420-w