-
Heliyon May 2024In metal-organic frameworks (MOFs), confined space as a chemical nanoreactor is as essential as coordinatively unsaturated metal site catalysis. The properties of MOFs...
In metal-organic frameworks (MOFs), confined space as a chemical nanoreactor is as essential as coordinatively unsaturated metal site catalysis. The properties of MOFs can be adjusted through the incorporation of functional groups and open metal sites in frameworks that can modify the catalytic performance. In this regard, a set of defect-engineered MOFs, Ex-MOF-808(NH, NO, H) and Mix-MOF-808(NH, NO, H), were synthesized by ultrasonic-assisted linker exchange approach (Ex-MOFs) and solvothermal mixing ligand method (Mix-MOFs), respectively. Further, the relationship between the preparation method, structural properties, and catalytic efficiency of the prepared materials in the selective oxidation of methyl phenyl sulfide (MPS) has been investigated. By analyzing zeta potential, it was found that in the exchange method, the amount of defect and functional groups on the surface of MOFs are more than in the mixing method, which also affects the catalytic activity. In our contribution, mix-MOF-808(NO) carrying nitro groups at their organic linkers, which has a well-dispersion of nitro groups at the framework exhibits selective conversion of MPS to sulfone (91 %). Furthermore, the performance of stable heterogeneous catalysts was investigated for three cycles, which demonstrated their great potential for advanced catalytic oxidation.
PubMed: 38813201
DOI: 10.1016/j.heliyon.2024.e31254 -
Scientific Reports May 2024Cryopreservation of sperm can cause oxidative stress and damage, leading to decreased different functional parameters and fertilization potential. In this study, we...
Cryopreservation of sperm can cause oxidative stress and damage, leading to decreased different functional parameters and fertilization potential. In this study, we evaluated two types of HS donors: NaHS, a fast-releasing donor, and GYY4137, a slow-releasing donor during cryopreservation of goat sperm. Initially, we determined that 1.5 and 3 μM NaHS, and 15 and 30 μM GYY4137 are optimal concentrations that improved different sperm functional parameters including motility, viability, membrane integrity, lipid peroxidation, and ROS production during incubation at 38.5 °C for 90 min. We subsequently evaluated the impact of the optimal concentration of NaHS and GYY4137 supplementation on various functional parameters following thawing during cryopreservation. Our data revealed that supplementation of extender improved different parameters including post-thaw sperm motility, viability, membrane integrity, and reduced DNA damage compared to the frozen-thawed control group. The supplementation also restored the redox state, decreased lipid peroxidation, and improved mitochondrial membrane potential in the thawed sperm. Finally, we found that supplementation of the extender with NaHS and GYY4137 enhanced IVF outcomes in terms of blastocyst rate and quality of blastocysts. Our results suggest that both donors can be applied for cryopreservation as antioxidants to improve sperm quality and IVF outcomes of frozen-thawed goat sperm.
Topics: Male; Cryopreservation; Animals; Oxidative Stress; Fertilization in Vitro; Spermatozoa; Goats; Sperm Motility; Semen Preservation; Organothiophosphorus Compounds; Lipid Peroxidation; Hydrogen Sulfide; Cryoprotective Agents; Cell Survival; Female; Reactive Oxygen Species; Membrane Potential, Mitochondrial; Semen Analysis; Morpholines; Sulfides
PubMed: 38811647
DOI: 10.1038/s41598-024-62485-2 -
Oncology Letters Jul 2024Esophagogastric junction adenocarcinoma (EJA) has increased in recent years, and it exhibits a poor prognosis and a short survival period for patients. Hydrogen sulfide...
Esophagogastric junction adenocarcinoma (EJA) has increased in recent years, and it exhibits a poor prognosis and a short survival period for patients. Hydrogen sulfide (HS) plays an important role in the pathogenesis of cancer and has been studied as a diagnostic factor in some tumor diseases. However, few studies have explored the diagnostic value of HS for EJA. In the present study, a total of 56 patients with early-stage EJA were enrolled while 57 healthy individuals were selected as the healthy control group. Clinical features were recorded, and exhaled HS and blood samples were collected from both groups. Exhaled HS and serum interleukin-8 (IL-8) expression levels were detected in both groups. The correlation between exhaled HS and serum IL-8 levels was analyzed using Pearson's correlation method. Receiver operating characteristic (ROC) curve was used to evaluate the diagnostic value of exhaled HS combined with IL-8 detection in EJA. The results showed that patients with EJA exhaled more HS than healthy individuals. In addition, exhaled HS was positively correlated with increased IL-8 expression. The ROC curve revealed that the exhaled HS test had an acceptable diagnostic effect and could be used to diagnose EJA. The increase in HS exhaled by patients with EJA indicated that HS may be related to the occurrence and development of EJA; however, the mechanism needs to be further explored. Collectively, it was determined in the present study that exhaled HS was significantly higher in patients with early-stage EJA than in healthy controls and combined diagnosis with patient serum IL-8 could improve diagnostic accuracy, which has potential diagnostic value for early diagnosis and screening of EJA.
PubMed: 38807679
DOI: 10.3892/ol.2024.14454 -
Journal of Neuroinflammation May 2024
Correction: Hydrogen sulfide-releasing cyclooxygenase inhibitor ATB-346 enhances motor function and reduces cortical lesion volume following traumatic brain injury in mice.
PubMed: 38807141
DOI: 10.1186/s12974-024-03131-6 -
Microbial Cell Factories May 2024This study aimed to investigate the operation of three parallel biotrickling filters (BTFs) in removing HS at different pH conditions (haloalkaliphilic, neutrophilic,...
This study aimed to investigate the operation of three parallel biotrickling filters (BTFs) in removing HS at different pH conditions (haloalkaliphilic, neutrophilic, and acidophilic) and their associated microbial population in the biodesulfurization process. BTF columns were inoculated with enriched inoculum and experiments were performed by gradually reducing Empty Bed Retention Time (EBRT) and increasing inlet concentration in which the maximum removal efficiency and maximum elimination capacity in EBRT 60 s reached their maximum level in haloalkaline condition (91% and 179.5 g S-HS m h). For visualizing the attached microbial biofilms on pall rings, Scanning Electron Microscopy (SEM) was used and microbial community structure analysis by NGS showed that the most abundant phyla in haBTF, nBTF, and aBTF belong to Gammaproteobacteria, Betaproteobacteria, and Acidithiobacillia, respectively. Shannon and Simpson indexes evaluation showed a lower diversity of bacteria in the aBTF reactor than that of nBTF and haBTF and beta analysis indicated a different composition of bacteria in haBTF compared to the other two filters. These results indicated that the proper performance of BTF under haloalkaliphilic conditions is the most effective way for HS removal from air pollutants of different industries.
Topics: Hydrogen-Ion Concentration; Hydrogen Sulfide; Biofilms; Bioreactors; Filtration; Bacteria; Air Pollutants; Biodegradation, Environmental; Betaproteobacteria
PubMed: 38807121
DOI: 10.1186/s12934-024-02427-9 -
Plant Cell Reports May 2024Sodium nitroprusside mediates drought stress responses in tomatoes by modulating nitrosative and oxidative pathways, highlighting the interplay between nitric oxide,...
Sodium nitroprusside mediates drought stress responses in tomatoes by modulating nitrosative and oxidative pathways, highlighting the interplay between nitric oxide, hydrogen sulfide, and antioxidant systems for enhanced drought tolerance. While nitric oxide (NO), a signalling molecule, enhances plant tolerance to abiotic stresses, its precise contribution to improving tomato tolerance to drought stress (DS) through modulating oxide-nitrosative processes is not yet fully understood. We aimed to examine the interaction of NO and nitrosative signaling, revealing how sodium nitroprusside (SNP) could mitigate the effects of DS on tomatoes. DS-seedlings endured 12% polyethylene glycol (PEG) in a 10% nutrient solution (NS) for 2 days, then transitioned to half-strength NS for 10 days alongside control plants. DS reduced total plant dry weight, chlorophyll a and b, Fv/Fm, leaf water potential (Ψ), and relative water content, but improved hydrogen peroxide (HO), proline, and NO content. The SNP reduced the DS-induced HO generation by reducing thiol (-SH) and the carbonyl (-CO) groups. SNP increased not only NO but also the activity of L-cysteine desulfhydrase (L-DES), leading to the generation of HS. Decreases in S-nitrosoglutathione reductase (GSNOR) and NADPH oxidase (NOX) suggest a potential regulatory mechanism in which -nitrosylation [formation of S-nitrosothiol (SNO)] may influence protein function and signaling pathways during DS. Moreover, SNP improved ascorbate (AsA) and glutathione (GSH) and reduced oxidized glutathione (GSSG) levels in tomato plants under drought. Furthermore, the interaction of NO and HS, mediated by L-DES activity, may serve as a vital cross-talk mechanism impacting plant responses to DS. Understanding these signaling interactions is crucial for developing innovative drought-tolerance strategies in crops.
Topics: Nitroprusside; Solanum lycopersicum; Hydrogen Peroxide; Droughts; Nitric Oxide; Glutathione; Antioxidants; Oxidation-Reduction; Oxidative Stress; Stress, Physiological; Seedlings; Plant Leaves; Nitrosation; Chlorophyll
PubMed: 38806834
DOI: 10.1007/s00299-024-03238-3 -
Nature Communications May 2024Herein, we disclose a highly efficient cobalt-catalyzed cross-electrophile alkynylation of a broad range of unactivated chlorosilanes with alkynyl sulfides as a stable...
Herein, we disclose a highly efficient cobalt-catalyzed cross-electrophile alkynylation of a broad range of unactivated chlorosilanes with alkynyl sulfides as a stable and practical alkynyl electrophiles. Strategically, employing easily synthesized alkynyl sulfides as alkynyl precursors allows access to various alkynylsilanes in good to excellent yields. Notably, this method avoids the utilization of strong bases, noble metal catalysts, high temperature and forcing reaction conditions, thus presenting apparent advantages, such as broad substrate scope (72 examples, up to 97% yield), high Csp-S chemo-selectivity and excellent functional group compatibility (Ar-X, X = Cl, Br, I, OTf, OTs). Moreover, the utilities of this method are also illustrated by downstream transformations and late-stage modification of structurally complex natural products and pharmaceuticals. Mechanistic studies elucidated that the cobalt catalyst initially reacted with alkynyl sulfides, and the activation of chlorosilanes occurred via an S2 process instead of a radical pathway.
PubMed: 38802390
DOI: 10.1038/s41467-024-48873-2 -
Saudi Journal of Biological Sciences Jul 2024The inorganic colour layer based on iron oxide is affected by microorganisms (fungi) and leads to its deterioration due to feeding on the mineral elements through the...
The inorganic colour layer based on iron oxide is affected by microorganisms (fungi) and leads to its deterioration due to feeding on the mineral elements through the chemical composition of the colour in the presence of a suitable environment (medium). Damage occurs as a result of heavy metal elements being removed from the colour, leading to a defect in the chemical composition and the fading of the colours. The current study showed the effect of the different types of the most common fungi on oil paintings ( and ) after cultivating the different types of fungi and obtaining pure colonies for each fungus separately and conducting a fungal infection on experimental samples with preparing the old techniques, coloured with hematite red and goethite yellow. Each colour is mixed with different proportions of linseed oil (1, 2, 3). They were aged artificially and incubated at a temperature of 26 degrees and examined periodically until the fungi appeared on the surface in the form of colour spots ranging from very dark (severe infestation) to light (low infestation). The change in chemical composition was measured by Raman and EDX analyses of the samples before and after infection. Fungi showed the appearance of spoilage products from metal sulfides and metal carboxylates. The iron oxide ion decreased in both the red and the yellow colours, leading to a change after the fungal infection. Examination of the morphological surface using SEM, USB and measurement of colour change showed the change in the red colour more than the yellow and scattering of green and black colour dots on the surface of the sample. Correlation and Simple Linear Regression were applied for each colour before and after besides both colours together. It was found that these colours appeared around some of the fungal colonies as a result of the activities. Fungal species of some strains reduced Fe to Fe . This provides new insights into the role of microorganisms in the deterioration of painted surfaces.
PubMed: 38799718
DOI: 10.1016/j.sjbs.2024.104004 -
Frontiers in Immunology 2024The new topical formula is urgent needed to meet clinical needs for majority mild patients with psoriasis. Deucravacitinib exerts outstanding anti-psoriatic capacity as...
Reactive oxygen species-responsive supramolecular deucravacitinib self-assembly polymer micelles alleviate psoriatic skin inflammation by reducing mitochondrial oxidative stress.
INTRODUCTION
The new topical formula is urgent needed to meet clinical needs for majority mild patients with psoriasis. Deucravacitinib exerts outstanding anti-psoriatic capacity as an oral TYK2 inhibitor; however, single therapy is insufficient to target the complicated psoriatic skin, including excessive reactive oxygen species (ROS) and persistent inflammation. To address this need, engineered smart nano-therapeutics hold potential for the topical delivery of deucravacitinib.
METHODS
hydrophobic Deucravacitinib was loaded into polyethylene glycol block-polypropylene sulphide (PEG-b-PPS) for transdermal delivery in the treatment of psoriasis. The oxidative stress model of HaCaT psoriasis was established by TNF-α and IL-17A . JC-1 assay, DCFH-DA staining and mtDNA copy number were utilized to assess mitochondrial function. 0.75% Carbopol934 was incorporated into SPMs to produce hydrogels and Rhb was labeled to monitor penetration by Immunofluorescence. , we established IMQ-induced psoriatic model to evaluate therapeutic effect of Car@Deu@PEPS.
RESULTS
Deu@PEPS exerted anti-psoriatic effects by restoring mitochondrial DNA copy number and mitochondrial membrane potential in HaCaT. , Car@Deu@PEPS supramolecular micelle hydrogels had longer retention time in the dermis in the IMQ-induced ROS microenvironment. Topical application of Car@Deu@PEPS significantly restored the normal epidermal architecture of psoriatic skin with abrogation of splenomegaly in the IMQ-induced psoriatic dermatitis model. Car@Deu@PEPS inhibited STAT3 signaling cascade with a corresponding decrease in the levels of the differentiation and proliferative markers Keratin 17 and Cyclin D1, respectively. Meanwhile, Car@Deu@PEPS alleviated IMQ-induced ROS generation and subsequent NLRP3 inflammasome-mediated pyroptosis.
CONCLUSION
Deu@PEPS exerts prominent anti-inflammatory and anti-oxidative effects, which may offers a more patient-acceptable therapy with fewer adverse effects compared with oral deucravacitinib.
Topics: Reactive Oxygen Species; Psoriasis; Humans; Oxidative Stress; Mitochondria; Micelles; Animals; Mice; Skin; Polymers; HaCaT Cells; Administration, Cutaneous; Male
PubMed: 38799436
DOI: 10.3389/fimmu.2024.1407782 -
ACS Omega May 2024The synthesis of InVO-CdS heterojunction photocatalysts has been achieved by a novel two-step approach, including a microwave-assisted technique, followed by a moderate...
The synthesis of InVO-CdS heterojunction photocatalysts has been achieved by a novel two-step approach, including a microwave-assisted technique, followed by a moderate hydrothermal method, marking the first successful instance of such a synthesis. X-ray diffraction, field-emission scanning electron microscopy, elemental color mapping, high-resolution transmission electron microscopy, UV-vis diffuse reflectance spectroscopy, Raman analysis, photoluminescence, X-ray photoelectron spectroscopy, and Brunauer-Emmett-Teller were employed to investigate the crystal structures, surface morphologies and particle sizes, chemical compositions, and optical characteristics of the as-synthesized materials. The research results indicated that the heterojunction InVO-CdS, as synthesized, consisted of InVO microrods with an average size of around 15 nm and cadmium sulfide (CdS) microflowers with a diameter of 1.5 μm. Furthermore, all of the heterojunctions had favorable photoabsorption properties throughout the visible-light spectrum. The photocatalytic efficiency of the samples obtained was thoroughly assessed by the degradation of acid violet 7 (AV 7) under visible light irradiation with a wavelength greater than 420 nm. The photocatalytic efficiency for the decomposition of AV 7 was greatly enhanced in the InVO-CdS (IVCS) heterojunctions when compared to prepared bare InVO and CdS. Additionally, it was observed that the composite material consisting of IVCS 3 wt % InVO combined with CdS exhibited the most significant enhancement in catalytic effectiveness for the photodegradation of AV 7 dye. Specifically, the catalytic performance of this composite material was found to be around 69.4 and 76.2 times greater than that of pure InVO and CdS, respectively. Furthermore, the experimental procedure including active species trapping provided evidence that h and •O radicals were the primary active species involved in the photocatalytic reaction process. Additionally, a potential explanation for the improved photocatalytic activity of the InVO-CdS heterojunction was presented, taking into account the determination of band positions.
PubMed: 38799365
DOI: 10.1021/acsomega.3c08850