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Clinical Microbiology and Infection :... Oct 2017
Topics: Acinetobacter; Acinetobacter Infections; Adult; Bacterial Proteins; Carbapenems; Child; Drug Resistance, Bacterial; Female; Humans; Male; Middle Aged; Netherlands; Sequence Analysis, DNA; beta-Lactamases
PubMed: 28487169
DOI: 10.1016/j.cmi.2017.04.024 -
Journal of Microbiology, Immunology,... Dec 2019Acinetobacter is an aerobic, gram-negative coccobacillus, which causes nosocomial infections including bacteremia. Recent development of molecular techniques has made... (Comparative Study)
Comparative Study
The clinical characteristics of Acinetobacter bacteremia differ among genomospecies: A hospital-based retrospective comparative analysis of genotypically identified strains.
BACKGROUND/PURPOSE
Acinetobacter is an aerobic, gram-negative coccobacillus, which causes nosocomial infections including bacteremia. Recent development of molecular techniques has made classification of the Acinetobacter genomospecies possible, but there are still only a few studies comparing clinical features of the subspecies. We investigated bacteremia caused by Acinetobacter, isolated subspecies, and compared clinical features for each group.
METHODS
A retrospective analysis of Acinetobacter bacteremia cases was made in a 900-bed hospital in Japan. In addition to conventional procedures, subspecies identification based on rpoB sequence was made, and comparison of clinical characteristics between each subspecies were analyzed.
RESULTS
We collected 35 cases (Acinetobacter baumannii 14, A. nosocomialis 12, Acinetobacter ursingii 6, and A. seifertii 3). All of the A. seifertii bacteremia cases were blood stream infection occurring in cerebrovascular disease patients, showing particularly higher incidence of shock (100%) and high Pitt bacteremia score (PBS) (6.33 ± 2.52) in comparison to A. baumannii (43% and 2.86 ± 2.25, respectively). Sequential Organ Failure Assessment (SOFA) score and the PBS were slightly higher in A. nosocomialis in comparison to A. baumannii, and the 7 day mortality rate was higher in A. nosocomialis (25%) than in A. baumannii (7%), though this difference was not found to be significant.
CONCLUSIONS
A.seifertii, the recently defined novel species, showed distinctive clinical features of bacteremia. And, in contrast to previous studies, the severity of A. nosocomialis infection was not lower than that of A. baumannii, which might suggest the influence of local epidemiology. Further characterization of these subspecies should be continued.
Topics: Acinetobacter; Acinetobacter Infections; Acinetobacter baumannii; Adult; Aged; Aged, 80 and over; Anti-Bacterial Agents; Bacteremia; Bacterial Proteins; Cross Infection; Female; Genotype; Hospitals; Humans; Japan; Male; Microbial Sensitivity Tests; Middle Aged; Retrospective Studies; Young Adult
PubMed: 31813733
DOI: 10.1016/j.jmii.2019.09.007 -
New Microbes and New Infections Mar 2018species can be important opportunistic pathogens in humans, especially in healthcare settings. We report here the first isolation of from an animal species; it was...
species can be important opportunistic pathogens in humans, especially in healthcare settings. We report here the first isolation of from an animal species; it was isolated from a canine urinary tract infection, and phenotypic identification proved unreliable.
PubMed: 29556400
DOI: 10.1016/j.nmni.2017.11.007 -
Microbial Pathogenesis Feb 2019The study aims to explore the characteristic microorganisms of the common tongue coatings in patients with gastric cancer (GC).
PURPOSE
The study aims to explore the characteristic microorganisms of the common tongue coatings in patients with gastric cancer (GC).
METHODS
A total of 115 GC patients were assigned to four groups: White-thin coating (W-thin) group, White-thick coating (W-thick) group, Yellow-thin coating (Y-thin) group and Yellow-thick coating (Y-thick) group. Thirty-five healthy volunteers with White-thin coating were recruit as controls. High-throughput sequencing was used to describe the microbial community of the tongue coatings based on 16S rRNA and 18S rRNA genes. Multi-factors statistical analysis was carried out to present the microbial biomarkers of the tongue coating in GC patients.
RESULTS
At bacterial phylum level, Saccharibacteria had higher relative abundance in W-thick group than W-thin group, Proteobacteria was more abundant in W-thin group than Y-thick group and less abundant in Y-thick group than Y-thin group. At fungal genus level, Guehomyces and Aspergillus presented to be significantly different among the common tongue coatings. Forteen significantly increased taxa were sorted out as the microbial biomarkers of common tongue coatings by LEfSe and ROC analysis. At species level, bacterial Capnocytophaga leadbetteri and fungal Ampelomyces_sp_IRAN_1 may be the potential biomarkers of W-thin coating, four bacterial species (Megasphaera micronuciformis, Selenomonas sputigena ATCC 35185, Acinetobacter ursingii, Prevotella maculosa) may be the potential biomarkers of W-thick coating. In general, the white coatings held more complex commensal relationship than the yellow coatings.
CONCLUSION
The common tongue coating owned characteristic microorganisms and special commensal relationship in the GC patients.
Topics: Aged; Bacteria; Cluster Analysis; DNA, Bacterial; DNA, Fungal; DNA, Ribosomal; Female; Fungi; Humans; Male; Microbiota; Middle Aged; Phylogeny; RNA, Ribosomal, 16S; RNA, Ribosomal, 18S; ROC Curve; Sequence Analysis, DNA; Stomach Neoplasms; Tongue
PubMed: 30508628
DOI: 10.1016/j.micpath.2018.11.051 -
Journal of Clinical Microbiology Aug 2008
Topics: Acinetobacter; Acinetobacter Infections; Bacteremia; Bacterial Typing Techniques; Cross Infection; DNA, Bacterial; DNA, Ribosomal; Humans; Nucleic Acid Hybridization; RNA, Ribosomal, 16S; Sequence Analysis, DNA
PubMed: 18678654
DOI: 10.1128/JCM.01003-08 -
Emerging Infectious Diseases Jan 2016We report a community-acquired bloodstream infection with Acinteobacter ursingii in an HIV-negative woman who injected drugs. The infection was successfully treated with...
We report a community-acquired bloodstream infection with Acinteobacter ursingii in an HIV-negative woman who injected drugs. The infection was successfully treated with meropenem. Species identification was performed by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Improved identification of Acinetobacter spp. by using this method will help identify clinical effects of this underdiagnosed pathogen.
Topics: Acinetobacter; Acinetobacter Infections; Bacteremia; Community-Acquired Infections; Female; Humans; Meropenem; Middle Aged; Thienamycins
PubMed: 26689082
DOI: 10.3201/eid2201.151298 -
The Pediatric Infectious Disease Journal Mar 2020
Topics: Acinetobacter; Acinetobacter Infections; Communicable Diseases, Emerging; Critical Care; Cross Infection; Humans; Infant; Infant, Newborn; Intensive Care, Neonatal; Premature Birth; Spain
PubMed: 32032315
DOI: 10.1097/INF.0000000000002562 -
Applied and Environmental Microbiology Nov 2014Many Gram-negative bacteria use N-acyl-l-homoserine lactones (AHLs) as quorum-sensing signal molecules. We have reported that Acinetobacter strains isolated from...
Many Gram-negative bacteria use N-acyl-l-homoserine lactones (AHLs) as quorum-sensing signal molecules. We have reported that Acinetobacter strains isolated from activated sludge have AHL-degrading activity. In this study, we cloned the amiE gene as an AHL-degradative gene from the genomic library of Acinetobacter sp. strain Ooi24. High-performance liquid chromatography analysis revealed that AmiE functions as an AHL acylase, which hydrolyzes the amide bond of AHL. AmiE showed a high level of degrading activity against AHLs with long acyl chains but no activity against AHLs with acyl chains shorter than eight carbons. AmiE showed homology with a member of the amidases (EC 3.5.1.4) but not with any known AHL acylase enzymes. An amino acid sequence of AmiE from Ooi24 showed greater than 99% identities with uncharacterized proteins from Acinetobacter ursingii CIP 107286 and Acinetobacter sp. strain CIP 102129, but it was not found in the draft or complete genome sequences of other Acinetobacter strains. The presence of transposase-like genes around the amiE genes of these three Acinetobacter strains suggests that amiE is transferred by a putative transposon. Furthermore, the expression of AmiE in Pseudomonas aeruginosa PAO1 reduced AHL accumulation and elastase activity, which were regulated by AHL-mediated quorum sensing.
Topics: Acinetobacter; Acyl-Butyrolactones; Amidohydrolases; Bacterial Proteins; Cloning, Molecular; Gene Expression Regulation, Bacterial; Molecular Sequence Data; Phylogeny; Sewage; Transposases
PubMed: 25172868
DOI: 10.1128/AEM.02190-14