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Clinical Microbiology and Infection :... Apr 2014Accurate and timely identification of anaerobic bacteria is critical to successful treatment. Classic phenotypic methods for identification require long turnaround times...
Accurate and timely identification of anaerobic bacteria is critical to successful treatment. Classic phenotypic methods for identification require long turnaround times and can exhibit poor species level identification. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is an identification method that can provide rapid identification of anaerobes. We present a multi-centre study assessing the clinical performance of the VITEK(®) MS in the identification of anaerobic bacteria. Five different test sites analysed a collection of 651 unique anaerobic isolates comprising 11 different genera. Multiple species were included for several of the genera. Briefly, anaerobic isolates were applied directly to a well of a target plate. Matrix solution (α-cyano-4-hydroxycinnamic acid) was added and allowed to dry. Mass spectra results were generated with the VITEK(®) MS, and the comparative spectral analysis and organism identification were determined using the VITEK(®) MS database 2.0. Results were confirmed by 16S rRNA gene sequencing. Of the 651 isolates analysed, 91.2% (594/651) exhibited the correct species identification. An additional eight isolates were correctly identified to genus level, raising the rate of identification to 92.5%. Genus-level identification consisted of Actinomyces, Bacteroides and Prevotella species. Fusobacterium nucleatum, Actinomyces neuii and Bacteroides uniformis were notable for an increased percentage of no-identification results compared with the other anaerobes tested. VITEK(®) MS identification of clinically relevant anaerobes is highly accurate and represents a dramatic improvement over other phenotypic methods in accuracy and turnaround time.
Topics: Bacteria, Anaerobic; Bacterial Infections; Bacteriological Techniques; Humans; Sensitivity and Specificity; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
PubMed: 23927597
DOI: 10.1111/1469-0691.12317 -
BMJ Case Reports May 2014We present the first reported case of ventriculoperitoneal shunt infection secondary to Actinomyces neuii in a paediatric patient. Our patient was managed with temporary...
We present the first reported case of ventriculoperitoneal shunt infection secondary to Actinomyces neuii in a paediatric patient. Our patient was managed with temporary shunt removal, intrathecal antibiotics and a prolonged course of intravenous and then oral antibiotics. She went on to make a complete recovery. Subsequent cerebrospinal fluid analysis at 5 months post-treatment demonstrated no evidence of residual infection.
Topics: Actinomyces; Actinomycosis; Anti-Bacterial Agents; Device Removal; Drainage; Female; Humans; Infant; Injections, Spinal; Penicillins; Prosthesis-Related Infections; Ventriculoperitoneal Shunt
PubMed: 24859562
DOI: 10.1136/bcr-2014-204576 -
Peritoneal Dialysis International :... 2014
Topics: Actinomyces; Aged, 80 and over; Anti-Bacterial Agents; Device Removal; Drug Therapy, Combination; Female; Follow-Up Studies; Humans; Infusions, Parenteral; Peritoneal Dialysis, Continuous Ambulatory; Peritonitis; Rare Diseases; Treatment Outcome
PubMed: 25520492
DOI: 10.3747/pdi.2013.00146 -
The ISME Journal May 2019Bacterial vaginosis (BV) is characterized by a highly structured polymicrobial biofilm, which is strongly adhered to the vaginal epithelium and primarily consists of the...
Bacterial vaginosis (BV) is characterized by a highly structured polymicrobial biofilm, which is strongly adhered to the vaginal epithelium and primarily consists of the bacterium Gardnerella vaginalis. However, despite the presence of other BV-associated bacteria, little is known regarding the impact of other species on BV development. To gain insight into BV progress, we analyzed the ecological interactions between G. vaginalis and 15 BV-associated microorganisms using a dual-species biofilm model. Bacterial populations were quantified using a validated peptide nucleic acid fluorescence in situ hybridization approach. Furthermore, biofilm structure was analyzed by confocal laser scanning microscopy. In addition, bacterial coaggregation ability was determined as well as the expression of key virulence genes. Remarkably, our results revealed distinct biofilm structures between each bacterial consortium, leading to at least three unique dual-species biofilm morphotypes. Furthermore, our transcriptomic findings seem to indicate that Enterococcus faecalis and Actinomyces neuii had a higher impact on the enhancement of G. vaginalis virulence, while the other tested species had a lower or no impact on G. vaginalis virulence. This study casts a new light on how BV-associated species can modulate the virulence aspects of G. vaginalis, contributing to a better understanding of the development of BV-associated biofilms.
Topics: Bacterial Proteins; Biofilms; Enterococcus faecalis; Female; Gardnerella vaginalis; Humans; In Situ Hybridization, Fluorescence; Microbiota; Vagina; Vaginosis, Bacterial; Virulence Factors
PubMed: 30670827
DOI: 10.1038/s41396-018-0337-0 -
Prosthetic valve endocarditis due to Actinomyces neuii successfully treated with antibiotic therapy.Journal of Clinical Microbiology Mar 2010Endocarditis due to Actinomyces neuii is a rare disease, with only 14 reported cases. Recently, A. neuii was added to the list of species implicated in endocarditis of...
Endocarditis due to Actinomyces neuii is a rare disease, with only 14 reported cases. Recently, A. neuii was added to the list of species implicated in endocarditis of native valves. We now report the first case of prosthetic valve endocarditis and the first successful control of endocarditis caused by this organism without surgical intervention.
Topics: Actinomyces; Actinomycosis; Aged; Anti-Bacterial Agents; Echocardiography, Transesophageal; Endocarditis, Bacterial; Humans; Male; Prosthesis-Related Infections; Treatment Outcome
PubMed: 20032249
DOI: 10.1128/JCM.01106-09 -
Le Infezioni in Medicina Jun 2004We report data concerning our experience during three years (1998-2001) about isolation, identification and susceptibility towards antimicrobial agents of coryneform...
We report data concerning our experience during three years (1998-2001) about isolation, identification and susceptibility towards antimicrobial agents of coryneform bacteria in infections of hospitalized/at risk patients. We isolated 54 Corynebacterium spp., with prevalence of C. striatum (8 strains) and C. amycolatum (7 strains), and 1 strain of Oerskovia spp. and 1 strain of Actinomyces neuii. 31 strains were isolated from the "exit-site" and 6 from peritoneal fluid of CAPD patients. Vancomycin and teicoplanin were always "in vitro" efficacious. Resistance rates towards other antibiotics were the following: 11% for minocycline, 12.5% for tetracycline, 20% for gentamicin and netilmicin, 61% for erythromycin and chloramphenicol, 66% for penicillin.
Topics: Actinomycetales; Actinomycetales Infections; Drug Resistance; Hospitals, University; Humans; Italy; Retrospective Studies; Species Specificity
PubMed: 15316299
DOI: No ID Found -
Microbial Ecology in Health and Disease 2014Coryneform bacteria constitute an important segment of male urogenital microbiota. They have been generally considered as saprophytes, although some species have been...
BACKGROUND
Coryneform bacteria constitute an important segment of male urogenital microbiota. They have been generally considered as saprophytes, although some species have been associated with prostatitis as well. At the same time, biofilm infections have been suspected as a cause of prostatitis.
OBJECTIVE
To identify a set of coryneform bacteria isolated from semen of either healthy men or prostatitis patients applying different methods to reveal inter-assay variability and to determine their ability of adhesion and biofilm production.
DESIGN
Coryneform bacteria were identified by API Coryne 2.0 biochemical identification system and 16S rDNA sequencing using different primer sets. Quantitative assessment of biofilm production was performed using crystal violet binding assay method.
RESULTS
The most common species were Corynebacterium seminale, C. minutissimum, and Dermabacter hominis. Altogether 14 species and related genera were found. We observed the best inter-assay agreement when identifying C. seminale. Biofilm was observed in 7 out of 24 strains. The biofilm-producing strains belonged to Arthrobacter cumminsii, Dermabacter hominis, C. minutissimum, and Actinomyces neuii. No differences were found between the strains originating from prostatitis patients and healthy men. Dermabacter hominis strains were more potent biofilm producers than C. seminale strains (p=0.048).
CONCLUSIONS
We can conclude that a wide variety of coryneform bacteria can be found from the male genital tract, although their exact identification is problematic due to insufficient representation in databases. Nearly one third of the strains are able to form biofilm that may give them an advantage for surviving several host- and treatment-related conditions.
PubMed: 24563649
DOI: 10.3402/mehd.v25.22701 -
International Journal of Infectious... Jun 2015Breast implant infections are usually caused by Staphylococcus aureus and coagulase-negative staphylococci. Gram-negative bacilli are rarely reported to be involved in...
BACKGROUND
Breast implant infections are usually caused by Staphylococcus aureus and coagulase-negative staphylococci. Gram-negative bacilli are rarely reported to be involved in breast implant infections.
METHODS
Thirty-seven cases of microbiologically confirmed breast implant infection managed from January 2008 to June 2012 in the study centre were reviewed, including 10 cases from the study centre itself and 27 cases from private clinics in the region.
RESULTS
The prevalence of breast implant infection in the study centre was 0.74% of breast implantation, i.e., 3.23% in breast reconstruction for breast cancer and 0.27% in aesthetic breast augmentation (p=0.0002). Of the 37 cases, 30% had undergone radiotherapy and 11% had undergone a lymph node dissection. S. aureus was identified in 18 cases, Gram-negative bacilli in 10 cases, coagulase-negative staphylococci in eight cases, anaerobic bacteria in eight cases, and streptococci in three cases. Pseudomonas aeruginosa was the second most commonly identified pathogen. Staphylococcus epidermidis was the most frequent coagulase-negative Staphylococcus species. In addition to Propionibacterium acnes and Actinomyces neuii, other facultative and strict anaerobic bacteria have not been reported before, e.g., Bacteroides thetaiotaomicron, Corynebacterium simulans, Dermabacter hominis, Finegoldia magna, and Peptoniphilus harei. Seventy-percent of cases were treated by immediate implant removal. All cases treated only with antibiotics were treated with surgery at the second visit.
CONCLUSIONS
The microbiological epidemiology was noted by an increasing the proportion of Gram-negative bacteria and anaerobic bacteria detected with the advent of MALDI-TOF MS and molecular identification for diagnosis.
Topics: Adult; Aged; Bacteria; Bacterial Infections; Breast Diseases; Breast Implants; Female; France; Gram-Negative Bacteria; Humans; Mammaplasty; Middle Aged; Postoperative Complications; Pseudomonas aeruginosa; Referral and Consultation; Staphylococcus; Staphylococcus aureus; Young Adult
PubMed: 25910855
DOI: 10.1016/j.ijid.2015.04.010 -
Journal de Gynecologie, Obstetrique Et... Feb 2010Primary actinomycosis of the breast is a rare disease, with only 32 cases reported in the literature since 1893. The diagnosis is done in front of a recurrent abscess...
Primary actinomycosis of the breast is a rare disease, with only 32 cases reported in the literature since 1893. The diagnosis is done in front of a recurrent abscess with fistulas and purulent discharge. Bacteriologic analysis only can confirm the diagnosis. We report the first case of primary actinomycosis of the breast caused by Actinomyces neuii, in a 46-year-old premenopausal woman. No cause of this condition was reported. Targeted antibiotic therapy did not improve the condition. A tumorectomy of the breast was decided. Six months after surgery, no recurrence has been observed.
Topics: Abscess; Actinomyces; Actinomycosis; Anti-Bacterial Agents; Breast Diseases; Female; Humans; Middle Aged; Recurrence
PubMed: 19819646
DOI: 10.1016/j.jgyn.2009.09.005 -
Journal of Clinical Microbiology Apr 2010
Topics: Actinomyces; Actinomycosis; Adult; Bacterial Typing Techniques; DNA, Bacterial; DNA, Ribosomal; Female; Humans; Male; Middle Aged; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Soft Tissue Infections
PubMed: 20181910
DOI: 10.1128/JCM.02139-09