-
The Journal of Experimental Medicine Mar 1964The electrophoretic mobilities of guinea pig gamma(1) and gamma(2) antibodies bearing different specificities were compared in agar gel at pH 8.2. Specifically purified...
The electrophoretic mobilities of guinea pig gamma(1) and gamma(2) antibodies bearing different specificities were compared in agar gel at pH 8.2. Specifically purified antibodies bearing the same immunological specificity showed the same electrophoretic mobility, but significant differences in mobility were observed when antibodies with certain selected different specificities were compared. The specificity of the carrier protein appeared not to affect the mobilities of antihapten antibodies. Differences in mobility have also been shown between gamma(1) antihapten antibodies produced by individual guinea pigs immunized concomitantly with 2,4-dinitrophenyl bovine gamma globulin and arsanilic acid azo guinea pig albumin. The differences in the net electrophoretic charge between antibodies with different specificities roughly paralleled that of their S fragments produced by papain digestion.
Topics: Animals; Antibodies; Blood Protein Electrophoresis; Cattle; Guinea Pigs; Immunoelectrophoresis; Papain; Research; gamma-Globulins
PubMed: 14129712
DOI: 10.1084/jem.119.3.409 -
The Journal of Experimental Medicine Oct 1983We describe a sequence of reciprocal interactions between cloned inducer T cells and antigen-presenting cells (APC) that results in selective depletion of the...
We describe a sequence of reciprocal interactions between cloned inducer T cells and antigen-presenting cells (APC) that results in selective depletion of the antigen-reactive inducer cells. We show that corecognition of antigen and I-A by hapten-reactive inducer T cell clones results in (a) release of macrophage-activating factor (MAF) and other lymphokines, (b) expression of lytic activity by a subset of MAF-sensitized APC after triggering, and (3) lysis (mediated by the activated and triggered macrophage) of the inducer T cell clone and other cells in the vicinity. We suggest that this sequence of steps may limit the extent of macrophage-mediated tissue destruction by depleting the specific inducer T cell clones that initiate the response.
Topics: Animals; Arsanilic Acid; Cell Line; Clone Cells; Cytotoxicity, Immunologic; Epitopes; Female; Lymphocyte Cooperation; Lymphocyte Depletion; Lymphokines; Macrophage Activation; Macrophage-Activating Factors; Macrophages; Mice; Mice, Inbred A; Mice, Inbred BALB C; Mice, Inbred C57BL; Spleen; T-Lymphocytes
PubMed: 6194244
DOI: 10.1084/jem.158.4.1243 -
Canadian Journal of Veterinary Research... Jul 1986Swine dysentery did not recur during a nine week period after withdrawal of medication in swine fed ronidazole at a level of 60 parts per million of feed for ten weeks...
Swine dysentery did not recur during a nine week period after withdrawal of medication in swine fed ronidazole at a level of 60 parts per million of feed for ten weeks or fed either carbadox at 55 ppm or lincomycin at 110 ppm of feed for six weeks. During this period swine dysentery was neither transmitted to accompanying sentinels after the withdrawal of the above medication or was Treponema hyodysenteriae isolated and cultured or observed in stained smears from rectal swabs and feces or from colonic scrapings at necropsy. Beginning three weeks after the withdrawal of medication, all swine were fed sodium arsanilate at a concentration of 220 ppm of feed for three weeks in an attempt to excite the carrier of swine dysentery into developing a swine dysentery diarrhea. A swine dysentery diarrhea did recur during the feeding of sodium arsanilate in swine previously fed ronidazole at a level of 60 ppm of feed for only six weeks. It was concluded: that swine dysentery was probably eliminated with the feeding of ronidazole for the longer duration and with the feeding of carbadox and lincomycin and that sodium arsanilate was of value in identifying the carrier state.
Topics: Animals; Arsanilic Acid; Arsenicals; Carbadox; Carrier State; Dysentery; Lincomycin; Nitroimidazoles; Quinoxalines; Ronidazole; Swine; Swine Diseases; Treponemal Infections
PubMed: 3742373
DOI: No ID Found -
Microbiology and Immunology 1978it was shown in our previous paper that mice primed with chemically modified bacterial alpha-amylase (BaA), which was neither cross-reactive with anti-BaA antibody nor... (Comparative Study)
Comparative Study
it was shown in our previous paper that mice primed with chemically modified bacterial alpha-amylase (BaA), which was neither cross-reactive with anti-BaA antibody nor able to induce a humoral anti-BaA response, developed enhanced responses to a subsequent challenge with native BaA and that the magnitude of the immunological memory was closely related to the priming dose of modified BaA. This paper describes the experimental conditions for induction of delayed hypersensitivity (DH) by modified BaA in relation to the development of immunological memory for antibody response to native BaA. Mice primed with either an intraperitoneal (i.p.) or subcutaneous (s.c.) injection of modified BaA in complete Freunds adjuvant (CFA) developed enhanced anti-BaA as the immunogen and modified BaA as the eliciting antigen, the relationship of anti-BaA responses to a subsequent challenge with BaA. In contrast, when mice were immunized with an s.c. injection of the modified BaA only, a significant level of DH to native BaA could be induced, as measured by the footpad reaction (FPR). The highest degree of DH was observed in mice given 50 micrograms of modified BaA. DH was detectable within 5 days and persisted for 25 days after immunization. In the reciprocal combination of native BaA as the immunogen and modified BaA as the eliciting antigen, the relationship of anti-BaA responses to DH was examined. The primary anti-BaA responses induced by an i.p. injection of large doses of BaA was markedly higher than those induced by an s.c. injection, while DH was exhibited only in mice given s.c. injection of BaA in CFA. With respect to DH to native BaA induced by the modified BaA, it was shown that C3H/He mice were high and C57BL/6 mice were low responders.
Topics: Amylases; Animals; Antibodies, Bacterial; Antigens, Bacterial; Arsanilic Acid; Bacillus subtilis; Female; Hypersensitivity, Delayed; Immunologic Memory; Methylation; Mice; alpha-Amylases
PubMed: 99645
DOI: 10.1111/j.1348-0421.1978.tb00365.x -
Infection and Immunity Nov 1970The immunogenicity, in rabbits, of homologous encephalitogenic brain basic protein (BP) can be enhanced by conjugating BP with three to five diazotized arsanilic or...
The immunogenicity, in rabbits, of homologous encephalitogenic brain basic protein (BP) can be enhanced by conjugating BP with three to five diazotized arsanilic or sulfanilic acid groups per molecule.
PubMed: 16557896
DOI: 10.1128/iai.2.5.676-678.1970 -
The Journal of Experimental Medicine May 1981Painting mice on the skin with the diazonium salt of p-arsanilic acid elicited two types of T cell activity. One was restricted by the I region of the major...
Painting mice on the skin with the diazonium salt of p-arsanilic acid elicited two types of T cell activity. One was restricted by the I region of the major histocompatibility complex and was responsible for the transfer of azobenzenearsonate (ABA) sensitivity to naive mice. The other was H-2K restricted and could be demonstrated by its ability to interact specifically with ABA-coupled cells in vitro and to inhibit nonspecifically the transfer of sensitivity by cells sensitized either to ABA or to another antigen. Free antigen, or antibody directed against the cross-reactive idiotype on the anti-ABA antibodies of A/J mice, could inhibit the H-2K-restricted suppressive activity induced in the ABA immune A/J cells.
Topics: Animals; Antigens; Azo Compounds; Cross Reactions; H-2 Antigens; Haptens; Hypersensitivity, Delayed; Mice; Spleen; T-Lymphocytes; T-Lymphocytes, Regulatory; p-Azobenzenearsonate
PubMed: 6454743
DOI: 10.1084/jem.153.5.1124 -
The Indian Medical Gazette Feb 1950
Topics: Arsanilic Acid; Arsenic; Arsenicals
PubMed: 15415143
DOI: No ID Found -
Sheng Li Xue Bao : [Acta Physiologica... Apr 2006In order to understand whether some special amino acids in the medial vestibular nucleus (MVN) of rats are involved in the regulation of blood pressure, we used...
In order to understand whether some special amino acids in the medial vestibular nucleus (MVN) of rats are involved in the regulation of blood pressure, we used microdialysis technique and high performance liquid chromatography (HPLC) to measure the changes of glutamate and taurine in this central area. Acute hypotension was induced by hemorrhage from the femoral artery. It was observed that the basal release of glutamate and taurine in the MVN was stable about 90 min after the beginning of microdialysis. The basal release of glutamate was (18.96 +/- 0.27) pmol/sample (8 mul), and that of taurine was (7.73 +/- 0.05) pmol/sample (8 mul). Glutamate release increased (P<0.05) and taurine release reduced (P<0.05) in the MVN in the hemorrhage-induced acute hypotensive rats. Nevertheless, these changes were not observed in the hemorrhage-induced acute hypotensive rats which were pretreated by infusing 2% lidocaine into the middle ear or 100 mg arsanilic acid into the tympanic cavity. These results suggest that the hemorrhage-induced acute hypotention can influence the activity of the neurons in the MVN by the afferent impulses from vestibular organ, and that some special amino acid transmitters in the MVN are involved in this process.
Topics: Animals; Blood Pressure; Glutamic Acid; Hypotension; Male; Microdialysis; Rats; Rats, Wistar; Taurine; Vestibular Nuclei
PubMed: 16628366
DOI: No ID Found -
Poultry Science Jul 1966
Topics: Aminobenzoates; Animals; Arsenicals; Body Weight; Dietary Proteins; Eggs; Poultry
PubMed: 6007821
DOI: 10.3382/ps.0450838 -
The Journal of Biological Chemistry Dec 1979
A peptidase-inactive derivative of carboxypeptidase A modified specifically at tyrosine 248. Cobalt(III) (ethylenediamine-N,N'-diacetato) (arsanilazotyrosinato 248 carboxypeptidase A).
Topics: Animals; Arsanilic Acid; Binding, Competitive; Carboxypeptidases; Cattle; Cobalt; Ethylenediamines; Kinetics; Pancreas; Structure-Activity Relationship; Tyrosine
PubMed: 574142
DOI: No ID Found