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The Biochemical Journal Jul 1940
Studies in the biochemistry of micro-organisms: The molecular constitutions of catenarin and erythroglaucin, metabolic products respectively of Helminthosporium catenarium Drechsler and of species in the Aspergillus glaucus series.
PubMed: 16747256
DOI: 10.1042/bj0341124 -
Annals of Agricultural and... 2006The concentration and composition of fungal flora in dental unit waterlines (DUWL) were evaluated. For this purpose, water samples from unit reservoirs and high-speed...
The concentration and composition of fungal flora in dental unit waterlines (DUWL) were evaluated. For this purpose, water samples from unit reservoirs and high-speed handpieces, and biofilm samples from the waterline walls from units were collected. Subsequently, analogous samples from DUWL were taken before and after disinfection using agent containing hydrogen peroxide. In the examined samples, the yeast-like fungi Candida albicans and Candida curvata were found. The following species of mould were also identified: Aspergillus amstelodami, Aspergillus fumigatus, Aspergillus glaucus group, Aspergillus (=Eurotium herbariorum) repens, Citromyces spp., Geotrichum candidum, Penicillium (glabrum) frequentans, Penicillium pusillum, Penicillium turolense and Sclerotium sclerotiorum (Sclerotinia sclerotiorum). Before disinfection, Candida curvata and Candida albicans constituted the greatest proportion of the total fungi in the reservoirs water; in the water of handpieces--Candida albicans and Aspergillus glaucus group; and in the biofilm samples--Aspergillus glaucus group and Candida albicans. After disinfection, in all 3 kinds of samples, Candida albicans prevailed, constituting from 31.2-85.7 % of the total fungi. The application of agent containing hydrogen peroxide caused a significant decrease both in the number of total fungi and individual fungal species, which confirms the product effectiveness in fungal decontamination of DUWL.
Topics: Antifungal Agents; Colony Count, Microbial; Dental Disinfectants; Dental Equipment; Dental Instruments; Disinfectants; Humans; Hydrogen Peroxide; Infection Control, Dental; Mitosporic Fungi; Poland; Water Microbiology; Water Purification
PubMed: 17196007
DOI: No ID Found -
TheScientificWorldJournal 2014The halotolerant fungus Aspergillus glaucus CCHA was isolated from the surface of wild vegetation around a saltern with the salinity range being 0-31%. Here, a...
The halotolerant fungus Aspergillus glaucus CCHA was isolated from the surface of wild vegetation around a saltern with the salinity range being 0-31%. Here, a full-length cDNA library of A. glaucus under salt stress was constructed to identify genes related to salt tolerance, and one hundred clones were randomly selected for sequencing and bioinformatics analysis. Among these, 82 putative sequences were functionally annotated as being involved in signal transduction, osmolyte synthesis and transport, or regulation of transcription. Subsequently, the cDNA library was transformed into E. coli cells to screen for putative salt stress-related clones. Five putative positive clones were obtained from E. coli cells grown on LB agar containing 1 M NaCl, on which they showed rapid growth compared to the empty vector control line. Analysis of transgenic Arabidopsis thaliana lines overexpressing CCHA-2142 demonstrated that the gene conferred increased salt tolerance to plants as well by protecting the cellular membranes, suppressing the inhibition of chlorophyll biosynthesis. These results highlight the utility of this A. glaucus cDNA library as a tool for isolating and characterizing genes related to salt tolerance. Furthermore, the identified genes can be used for the study of the underlying biology of halotolerance.
Topics: Arabidopsis; Aspergillus; Carbohydrates; Caseins; Escherichia coli; Gene Expression Regulation, Fungal; Gene Library; Lipids; Plant Proteins, Dietary; Plants, Genetically Modified; Salinity; Salt Tolerance; Signal Transduction; Sodium Chloride; Stress, Physiological
PubMed: 25383373
DOI: 10.1155/2014/620959 -
Medical Mycology Case Reports 2012Aspergillus are ubiquitous and more than 30 species have been reported to be involved in human infection. Most of the cases occur in immunocompromised patients and are...
Aspergillus are ubiquitous and more than 30 species have been reported to be involved in human infection. Most of the cases occur in immunocompromised patients and are disseminated in the blood. Primary cutaneous aspergillosis in immunocompetent hosts is rare. We report a unique case of primary cutaneous aspergillosis in an immunocompetent patient diagnosed by fine needle aspiration cytology. The characteristic ascocarp and ascospores of Aspergillus species were found in the aspirate and Aspergillus glaucus was isolated in pure culture. The case is presented to increase the awareness of the usefulness of fine needle aspiration cytology for diagnosing fungal infections.
PubMed: 24371752
DOI: 10.1016/j.mmcr.2012.09.004 -
International Journal of Molecular... Jan 2015High salt in soils is one of the abiotic stresses that significantly reduces crop yield, although saline lands are considered potential resources arable for agriculture....
High salt in soils is one of the abiotic stresses that significantly reduces crop yield, although saline lands are considered potential resources arable for agriculture. Currently, genetic engineering for enhancing salt tolerance is being tested as an efficient and viable strategy for crop improvement. We previously characterized a large subunit of the ribosomal protein RPL44, which is involved in osmotic stress in the extremely halophilic fungus Aspergillus glaucus. Here, we screened another ribosomal protein (AgRPS3aE) that also produced high-salt tolerance in yeast. Bioinformatics analysis indicated that AgRPS3aE encodes a 29.2 kDa small subunit of a ribosomal protein belonging to the RPS3Ae family in eukaryotes. To further confirm its protective function against salinity, we expressed AgRPS3aE in three heterologous systems, the filamentous fungus Magnaporthe oryzae and two model plants Arabidopsis and tobacco. Overexpression of AgRPS3aE in all tested transformants significantly alleviated stress symptoms compared with controls, suggesting that AgRPS3aE functions not only in fungi but also in plants. Considering that ribosomal proteins are housekeeping components in organisms from prokaryotes to eukaryotes, we propose that AgRPS3aE is one of the optimal genes for improving high-salt tolerance in crops.
Topics: Amino Acid Sequence; Animals; Arabidopsis; Aspergillus; Cloning, Molecular; Evolution, Molecular; Fungal Proteins; Humans; Molecular Sequence Data; Osmotic Pressure; Phylogeny; Plants, Genetically Modified; Ribosomal Proteins; Salt Tolerance; Seedlings; Sequence Alignment; Nicotiana
PubMed: 25642759
DOI: 10.3390/ijms16023058 -
International Journal of Molecular... Feb 2023Scientific examination of the heart of Blessed Pauline Jaricot-a French missionary figure-was carried out in 2022. As tandem mass spectrometry proteotyping has proven to...
Scientific examination of the heart of Blessed Pauline Jaricot-a French missionary figure-was carried out in 2022. As tandem mass spectrometry proteotyping has proven to be valuable to obtain the broad taxonomic repertoire of a given sample without any a priori information, we aimed at exploring the conditions of preservation of the relics and possible conditions of death. Metaproteomics and high-resolution microtomography imaging approaches were combined. A dataset comprising 6731 high-resolution MS/MS spectra was acquired and 968 of these spectra could be assigned to specific peptidic biomolecules. Based on the taxonomical information encompassed by the identified peptide sequences, 5 phyla were identified amongst eukaryota (94% of the biomass): Ascomycota (55%), with the species , and , corresponding to expected cadaverous fungal flora; Chordata (42%), represented by a unique species, ; Streptophyta (3%); and Arthropoda (traces). Bacteria (6% of the biomass) were poorly represented. No trace of embalming substance could be retrieved, nor any pathogens. Imaging evidenced no heart defect nor embalming traces. No evidence that was inconsistent with natural and spontaneous conservation could be retrieved. This study prefigures the power of modern molecular techniques such as paleoproteotyping coupled to microtomography to gain insight into historical relics.
Topics: Humans; Embalming; Tandem Mass Spectrometry; Heart; Heart Defects, Congenital; Bacteria
PubMed: 36769339
DOI: 10.3390/ijms24033011 -
Molecules (Basel, Switzerland) Mar 2024A fungal isolate PDB-B (accession number: MT774567.1), which could tolerate up to 500 mg/L of cypermethrin, was isolated from the lake sediments of Kulamangalam...
A fungal isolate PDB-B (accession number: MT774567.1), which could tolerate up to 500 mg/L of cypermethrin, was isolated from the lake sediments of Kulamangalam tropical lake, Madurai, and identified by internal transcribed spacer (ITS) sequencing followed by phylogenetic analysis. The biotransformation potential of the strain was compared with five other strains (A, J, UN2, M1 and SM108) as a consortium, which were tentatively identified as , , , , and , respectively. Batch culture and soil microcosm studies were conducted to explore biotransformation using plate-based enzymatic screening and GC-MS. A mycotransformation pathway was predicted based on a comparative analysis of the transformation products (TPs) obtained. The cytotoxicity assay revealed that the presence of (3-methylphenyl) methanol and isopropyl ether could be relevant to the high rate of lethality.
Topics: Lakes; Phylogeny; India; Aspergillus niger; Aspergillus; Pyrethrins
PubMed: 38611726
DOI: 10.3390/molecules29071446 -
Journal of Clinical Microbiology Nov 1995We have developed 21 specific nucleic acid probes which target the large subunit rRNA genes from Aspergillus flavus, Aspergillus fumigatus, Aspergillus glaucus,... (Comparative Study)
Comparative Study
We have developed 21 specific nucleic acid probes which target the large subunit rRNA genes from Aspergillus flavus, Aspergillus fumigatus, Aspergillus glaucus, Aspergillus niger, Aspergillus terreus, Blastomyces dermatitidis, Candida albicans, Candida (Torulopsis) glabrata, Candida guilliermondii, Candida kefyr, Candida krusei, Candida lusitaniae, Candida parapsilosis, Candida tropicalis, Coccidioides immitis, Cryptococcus neoformans var. gattii, Cryptococcus neoformans var. neoformans, Filobasidiella neoformans var. bacillispora, Filobasidiella neoformans var. neoformans, Histoplasma capsulatum, Pseudallescheria boydii, and Sporothrix schenckii. A section of the 28S rRNA gene from approximately 100 fungi, representing about 50 species of pathogens and commonly encountered saprophytes, was sequenced to develop universal PCR primers and species-specific oligonucleotide probes. Each step in the process of detection and identification was standardized to a common set of conditions applicable without modification to all fungi of interest and all types of clinical specimens. These steps consist of DNA extraction by boiling specimens in an alkaline guanidine-phenol-Tris reagent, amplification of a variable region of the 28S rRNA gene with universal primers, and amplicon identification by probe hybridization or DNA sequencing performed under conditions identical for all fungi. The results obtained by testing a panel of fungal isolates and a variety of clinical specimens indicate a high level of specificity.
Topics: Base Sequence; DNA Primers; DNA, Fungal; DNA, Ribosomal; Fungi; Humans; Molecular Sequence Data; Mycoses; Oligonucleotide Probes; Polymerase Chain Reaction; RNA, Ribosomal, 28S; Retrospective Studies; Sensitivity and Specificity; Sequence Analysis, DNA; Species Specificity
PubMed: 8576345
DOI: 10.1128/jcm.33.11.2913-2919.1995 -
Foods (Basel, Switzerland) Sep 2023In order to analyze the changes in the microbial community structure during the pile fermentation of Qingzhuan tea and their correlation with the formation of quality...
In order to analyze the changes in the microbial community structure during the pile fermentation of Qingzhuan tea and their correlation with the formation of quality compounds in Qingzhuan tea, this study carried out metagenomic and metabolomic analyses of tea samples during the fermentation process of Qingzhuan tea. The changes in the expression and abundance of microorganisms during the pile fermentation were investigated through metagenomic assays. During the processing of Qingzhuan tea, there is a transition from a bacterial dominated ecosystem to an ecosystem enriched with fungi. The correlation analyses of metagenomics and metabolomics showed that amino acids and polyphenol metabolites with relatively simple structures exhibited a significant negative correlation with target microorganisms, while the structurally complicated B-ring dihydroxy puerin, B-ring trihydroxy galloyl puerlin, and other compounds showed a significant positive correlation with target microorganisms. , , in the family, and and in were the key microorganisms involved in the formation of the characteristic qualities of Qingzhuan tea.
PubMed: 37835190
DOI: 10.3390/foods12193537 -
Toxins Mar 2011Thesafety characteristics of feed used in fish and crustacean aquaculture systems are an essential tool to assure the productivity of those animal exploitations. Safety...
Thesafety characteristics of feed used in fish and crustacean aquaculture systems are an essential tool to assure the productivity of those animal exploitations. Safety of feed may be affected by different hazards, including biological and chemical groups. The aim of this preliminary study was to evaluate fungi contamination and the presence of aflatoxins in 87 samples of feed for sea bass, collected in Portugal. Molds were found in 35 samples (40.2%) in levels ranging from 1 to 3.3 log10 CFU∙g(-1). Six genera of molds were found. Aspergillus flavus was the most frequent, found in all positive samples, with a range from 2 to 3.2 log(10) CFU∙g(-1). Aspergillus niger was found in 34 samples (39.1%), ranging from 1 to 2.7 log(10) CFU∙g(-1). Aspergillus glaucus was found in 26 samples (29.9%) with levels between 1 and 2.4 log(10) CFU∙g(-1). Penicillium spp. and Cladosporium spp. were both found in 25 samples (28.7%). Fusarium spp. was found in 22 samples (25.3%), ranging from 1 to 2.3 log(10) CFU∙g(-1). All feed samples were screened for aflatoxins using a HPLC technique, with a detection limit of 1.0 μg∙kg(-1). All samples were aflatoxin negative.
Topics: Aflatoxin B1; Animal Feed; Animals; Aspergillus; Bass; Fisheries; Food Contamination; Portugal
PubMed: 22069703
DOI: 10.3390/toxins3030163