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Clinical Orthopaedics and Related... Oct 2012Cis-2 decenoic acid (C2DA) disperses biofilm in many strains of microorganisms. However, whether C2DA inhibits bacterial growth or has potential to boost the actions of...
BACKGROUND
Cis-2 decenoic acid (C2DA) disperses biofilm in many strains of microorganisms. However, whether C2DA inhibits bacterial growth or has potential to boost the actions of antibiotics is unknown.
QUESTIONS/PURPOSES
We asked whether (1) C2DA inhibited MRSA growth and biofilm, (2) antibiotics increased inhibitory effects, (3) inhibitory concentrations of C2DA were cytotoxic to human cells, and (4) effective concentrations could be delivered from a chitosan sponge drug delivery device.
METHODS
Broth containing seven concentrations of C2DA and six concentrations of either daptomycin, vancomycin, or linezolid was inoculated with a clinical isolate of MRSA and added to a total of 504 coated microtiter plate wells in triplicate (n = 3) for turbidity bacterial growth and crystal violet biofilm mass quantification. We used fibroblast cell viability assays of six C2DA concentrations (n = 4) to evaluate preliminary biocompatibility. We measured the elution of C2DA from a chitosan sponge drug delivery device with two representative loading concentrations (n = 3).
RESULTS
C2DA at concentrations of 500 μg/mL and above inhibited growth, while 125 μg/mL C2DA inhibited biofilm. Combination with antibiotics increased these effects. At concentrations up to 500 μg/mL, there were no cytotoxic effects on fibroblasts. Chitosan sponges loaded with 100 mg of C2DA eluted concentrations at or above biofilm-inhibitory concentrations for 5 days.
CONCLUSIONS
C2DA inhibited biofilm formation by MRSA at biocompatible concentrations, with increasing biofilm reduction with added antibiotics. Elution of C2DA from a chitosan sponge can be modified through adjusting loading concentration.
CLINICAL RELEVANCE
By inhibiting biofilm formation on implant surfaces, C2DA may reduce the number of infections in musculoskeletal trauma.
Topics: Biofilms; Fatty Acids, Monounsaturated; Humans; Methicillin-Resistant Staphylococcus aureus; Microbial Sensitivity Tests; Pilot Projects
PubMed: 22585351
DOI: 10.1007/s11999-012-2388-2 -
Animals : An Open Access Journal From... Jul 2022The purposes of this study were to explore the potential possibility of 10-hydroxy-trans-2-decenoic acid (10-HDA) use in feeding broiler chickens. A total of 216 healthy...
The purposes of this study were to explore the potential possibility of 10-hydroxy-trans-2-decenoic acid (10-HDA) use in feeding broiler chickens. A total of 216 healthy 1-day-old chicks were divided into 2 treatments at random (diets supplemented with 0 or 40 mg/kg 10-HDA, respectively) with 6 replicates of 18 birds each, and were then reared for 42 days. The results found that a diet supplemented with 10-HDA significantly increased average daily gain of broiler chickens in d 22~42 and d 0~42. Compared with the control group, dietary inclusion of 10-HDA markedly increased the serum concentrations of immunoglobulin (Ig) G at d 21, as well as IgM and interleukin (IL)-10 at d 42, while decreasing the levels of tumor necrosis factor (TNF)-α at d 21, as well as IL-6, TNF-α, and IL-1β at d 42. Furthermore, broiler chickens fed a diet with 10-HDA had a higher (p < 0.05) serum activity of superoxide dismutase at d 42. Additionally, serum malondialdehyde content also decreased notably at d 21 and d 42. These results made it clear that 10-HDA increased the growth performance of broiler chickens, possibly by enhancing immune function and antioxidant capacity.
PubMed: 35883394
DOI: 10.3390/ani12141846 -
Cell Biology and Toxicology Jun 2023Autophagy is a conserved intracellular catabolic pathway that removes cytoplasmic components to contribute to neuronal homeostasis. Accumulating evidence has...
Autophagy is a conserved intracellular catabolic pathway that removes cytoplasmic components to contribute to neuronal homeostasis. Accumulating evidence has increasingly shown that the induction of autophagy improves neuronal health and extends longevity in several animal models. Therefore, there is a great interest in the identification of effective autophagy enhancers with potential nutraceutical or pharmaceutical properties to ameliorate age-related diseases, such as neurodegenerative disorders, and/or promote longevity. Queen bee acid (QBA, 10-hydroxy-2-decenoic acid) is the major fatty acid component of, and is found exclusively in, royal jelly, which has beneficial properties for human health. It is reported that QBA has antitumor, anti-inflammatory, and antibacterial activities and promotes neurogenesis and neuronal health; however, the mechanism by which QBA exerts these effects has not been fully elucidated. The present study investigated the role of the autophagic process in the protective effect of QBA. We found that QBA is a novel autophagy inducer that triggers autophagy in various neuronal cell lines and mouse and fly models. The beclin-1 (BECN1) and mTOR pathways participate in the regulation of QBA-induced autophagy. Moreover, our results showed that QBA stimulates sirtuin 1 (SIRT1), which promotes autophagy by the deacetylation of critical ATG proteins. Finally, QBA-mediated autophagy promotes neuroprotection in Parkinson's disease in vitro and in a mouse model and extends the lifespan of Drosophila melanogaster. This study provides detailed evidences showing that autophagy induction plays a critical role in the beneficial health effects of QBA.
Topics: Mice; Humans; Bees; Animals; Neuroprotection; Drosophila melanogaster; Autophagy; Cell Line; Parkinson Disease; Neuroprotective Agents
PubMed: 34448959
DOI: 10.1007/s10565-021-09625-w -
Scientific Reports May 2021In bees from genus Melipona, differential feeding is not enough to fully explain female polyphenism. In these bees, there is a hypothesis that in addition to the...
In bees from genus Melipona, differential feeding is not enough to fully explain female polyphenism. In these bees, there is a hypothesis that in addition to the environmental component (food), a genetic component is also involved in caste differentiation. This mechanism has not yet been fully elucidated and may involve epigenetic and metabolic regulation. Here, we verified that the genes encoding histone deacetylases HDAC1 and HDAC4 and histone acetyltransferase KAT2A were expressed at all stages of Melipona scutellaris, with fluctuations between developmental stages and castes. In larvae, the HDAC genes showed the same profile of Juvenile Hormone titers-previous reported-whereas the HAT gene exhibited the opposite profile. We also investigated the larvae and larval food metabolomes, but we did not identify the putative queen-fate inducing compounds, geraniol and 10-hydroxy-2E-decenoic acid (10HDA). Finally, we demonstrated that the histone deacetylase inhibitor 10HDA-the major lipid component of royal jelly and hence a putative regulator of honeybee caste differentiation-was unable to promote differentiation in queens in Melipona scutellaris. Our results suggest that epigenetic and hormonal regulations may act synergistically to drive caste differentiation in Melipona and that 10HDA is not a caste-differentiation factor in Melipona scutellaris.
Topics: Acyclic Monoterpenes; Animals; Bees; Epigenesis, Genetic; Fatty Acids; Fatty Acids, Monounsaturated; Feeding Behavior; Female; Gene Expression Regulation, Developmental; Hierarchy, Social; Histone Acetyltransferases; Histone Deacetylases; Insect Proteins; Juvenile Hormones
PubMed: 33972627
DOI: 10.1038/s41598-021-89212-5 -
The Open Orthopaedics Journal 2014The demand for infection prevention therapies has led to the discovery of several biofilm inhibitors. These inhibiting signals are released by bacteria, fungi, or marine...
The demand for infection prevention therapies has led to the discovery of several biofilm inhibitors. These inhibiting signals are released by bacteria, fungi, or marine organisms to signal biofilm dispersal or disruption in Gram-positive, Gram-negative, and fungal microorganisms. The purpose of this study was to test the biocompatibility of five different naturally-produced biofilm chemical dispersal and inhibition signals with osteoblast-like cells: D-amino acids (D-AA), lysostaphin (LS), farnesol, cis-2-decenoic acid (C2DA), and desformyl flustrabromine (dFBr). In this preliminary study, compatibility of these anti-biofilm agents with differentiating osteoblasts was examined over a 21 days period at levels above and below concentrations active against bacterial biofilm. Anti-biofilm compounds listed above were serially diluted in osteogenic media and added to cultures of MC3T3 cells. Cell viability and cytotoxicity, after exposure to each anti-biofilm agent, were measured using a DNA assay. Differentiation characteristics of osteoblasts were determined qualitatively by observing staining of mineral deposits and quantitatively with an alkaline phosphatase assay. D-AA, LS, and C2DA were all biocompatible within the reported biofilm inhibitory concentration ranges and supported osteoblast differentiation. Farnesol and dFBr induced cytotoxic responses within the reported biofilm inhibitory concentration range and low doses of dFBr were found to inhibit osteoblast differentiation. At high concentrations, such as those that may be present after local delivery, many of these biofilm inhibitors can have effects on cellular viability and osteoblast function. Concentrations at which negative effects on osteoblasts occur should serve as upper limits for delivery to orthopaedic trauma sites and guide development of these potential therapeutics for orthopaedics.
PubMed: 25505496
DOI: 10.2174/1874325001408010442 -
Mediators of Inflammation 2016-10-hydroxy-2-decenoic acid (10-H2DA), 10-hydroxydecanoic acid (10-HDAA), and sebacic acid (SEA) are the three major fatty acids in royal jelly (RJ). Previous studies...
-10-hydroxy-2-decenoic acid (10-H2DA), 10-hydroxydecanoic acid (10-HDAA), and sebacic acid (SEA) are the three major fatty acids in royal jelly (RJ). Previous studies have revealed several pharmacological activities of 10-H2DA and 10-HDAA, although the anti-inflammatory effects and underlying mechanisms by which SEA acts are poorly understood. In the present study, we evaluated and compared the anti-inflammatory effects of these RJ fatty acids in lipopolysaccharide-stimulated RAW 264.7 macrophages. The results showed that 10-H2DA, 10-HDAA, and SEA had potent, dose-dependent inhibitory effects on the release of the major inflammatory-mediators, nitric oxide, and interleukin-10, and only SEA decreased TNF- production. Several key inflammatory genes have also been modulated by these RJ fatty acids, with 10-H2DA showing distinct modulating effects as compared to the other two FAs. Furthermore, we found that these three FAs regulated several proteins involved in MAPK and NF-B signaling pathways. Taken together, these findings provide additional references for using RJ against inflammatory diseases.
Topics: Animals; Anti-Inflammatory Agents; Cell Survival; Cytokines; Decanoic Acids; Dicarboxylic Acids; Fatty Acids; Fatty Acids, Monounsaturated; Inflammation; Lipopolysaccharides; MAP Kinase Signaling System; Macrophages; Mice; NF-kappa B; Nitric Oxide; RAW 264.7 Cells; Signal Transduction
PubMed: 27847405
DOI: 10.1155/2016/3583684 -
Advanced Pharmaceutical Bulletin Sep 2021Microbial biofilms are one of the main causes of persistent human infections. Encapsulation of an antibiotic and a biofilm dispersal agent within a nano-carrier has...
Microbial biofilms are one of the main causes of persistent human infections. Encapsulation of an antibiotic and a biofilm dispersal agent within a nano-carrier has been recognized as a novel approach to combat the problem of biofilm-related infections. Here, we develop the nanoliposomal formulation for delivery of vancomycin in combination with cis-2- decenoic acid (C2DA), to biofilm. The effects of the formulations were studied at two stages: biofilm growth inhabitation and biofilm eradication. Liposomal formulations were prepared by the solvent evaporation dehydration-rehydration method and were evaluated for size, zeta potential, and encapsulation efficacy. The ability of different agents in free and encapsulated forms were assessed to evaluate the anti-biofilm activities. Vancomycin and C2DA were successfully co-encapsulated in the same nanoliposome (liposomal combination). The zeta potential values of the liposomal formulations of vancomycin, C2DA, and the liposomal combination were 37.2, 40.2, 51.5 mV, and the mean sizes of these liposomal formulations were 167.8±1.5, 215.5±8.8, 235.5±0.01, respectively. Encapsulation efficacy of C2DA was 65% and about 40% for vancomycin. The results indicated that liposomal combination exerted strong anti-biofilm activities, slightly exceeding those observed by the free form of a combination of vancomycin and C2DA, but higher than either agent used alone in their free forms. The anti-biofilm activity of formulations followed concentration and time-dependent manner. The combination of vancomycin and C2DA could inhibit biofilm formation. Employing the liposomal combination is a considerable method to remove bacterial biofilm.
PubMed: 34888215
DOI: 10.34172/apb.2021.077 -
The Journal of Biological Chemistry Aug 2020The supplementation of royal jelly (RJ) is known to provide a variety of health benefits, including anti-inflammatory and anti-obesity effects. RJ treatment also...
The supplementation of royal jelly (RJ) is known to provide a variety of health benefits, including anti-inflammatory and anti-obesity effects. RJ treatment also reportedly protects against bone loss, but no single factor in RJ has yet been identified as an anti-osteoporosis agent. Here we fractionated RJ and identified 10-hydroxy-2-decenoic acid (10H2DA) as a key component involved in inhibiting osteoclastogenesis based on mass spectrometric analysis. We further demonstrated free fatty acid receptor 4 (FFAR4) as directly interacting with 10H2DA; binding of 10H2DA to FFAR4 on osteoclasts inhibited receptor activator of nuclear factor-κB (NF-κB) ligand (RANKL)-induced activation of NF-κB signaling, thereby attenuating the induction of nuclear factor of activated T cells (NFAT) c1, a key transcription factor for osteoclastogenesis. Oral administration of 10H2DA attenuated bone resorption in ovariectomized mice. These results suggest a potential therapeutic approach of targeting osteoclast differentiation by the supplementation of RJ, and specifically 10H2DA, in cases of pathological bone loss such as occur in postmenopausal osteoporosis.
Topics: Animals; Cell Differentiation; Disease Models, Animal; Fatty Acids; Fatty Acids, Monounsaturated; Female; Mice; NF-kappa B; NFATC Transcription Factors; Osteoclasts; Osteoporosis; RANK Ligand; Receptors, G-Protein-Coupled; Signal Transduction
PubMed: 32647011
DOI: 10.1074/jbc.RA120.013821 -
Zhongguo Yao Li Xue Bao = Acta... Mar 1997To study the effect of 10-hydroxy-2-decenoic acid (HDA) on the macrophages activity of rats in vitro.
AIM
To study the effect of 10-hydroxy-2-decenoic acid (HDA) on the macrophages activity of rats in vitro.
METHODS
To measure the effects of HDA on phagocytosis, the production of antitumor cytokines tumor necrosis factor (TNF) and interleukin-1 (IL-1) in vitro.
RESULTS
HDA 50, 100 mg.L-1 promoted phagocytic activity from 0.353 +/- 0.017 to 0.39 +/- 0.04 and 0.382 +/- 0.017 A, increased TNF and IL-1 production from 0.23 +/- 0.07% to 0.43 +/- 0.04%, 0.47 +/- 0.04% and from 2384 +/- 180 to 2943 +/- 295, 3825 +/- 450 dpm, respectively.
CONCLUSION
The upregulating effects of HDA on phagocytosis of PMO and production of TNF, IL-1 contribute to the host antitumor and immunomodulating mechanism.
Topics: Adjuvants, Immunologic; Animals; Antineoplastic Agents; Fatty Acids, Monounsaturated; Female; Interleukin-1; Macrophages, Peritoneal; Male; Mice; Mice, Inbred C57BL; Phagocytosis; Rats; Rats, Sprague-Dawley; Tumor Necrosis Factor-alpha
PubMed: 10072977
DOI: No ID Found -
Biomedical Research (Tokyo, Japan) Dec 2010We presently found that medium-chain fatty acids (MCFAs) with 8-12 carbons and their esters facilitated activation (phosphorylation) of mitogen-activated protein kinases...
2-Decenoic acid ethyl ester possesses neurotrophin-like activities to facilitate intracellular signals and increase synapse-specific proteins in neurons cultured from embryonic rat brain.
We presently found that medium-chain fatty acids (MCFAs) with 8-12 carbons and their esters facilitated activation (phosphorylation) of mitogen-activated protein kinases (MAPK)/extracellular signal-regulated kinases (ERK) 1/2 of cultured embryonic cortical/hippocampal neurons. In particular, trans-2-decenoic acid ethyl ester (DAEE) had the most potent activity. Additionally, DAEE activated phosphatidylinositol 3-kinase and cAMP-response element binding protein (CREB), suggesting that DAEE generates similar intracellular signal as neurotrophins. Therefore, details of the signal elicited by DAEE were examined in comparison with those of a neurotrophin, brain-derived neurotrophic factor (BDNF). We found that 1) DAEE phosphorylated MAPK/ERK1/2 via MEK activation without the involvement of tyrosine kinases of neurotrophin Trk receptors; 2) DAEE activated CREB predominantly through MAPK/ERK1/2 activation, not through other pathways such as cAMP/protein kinase A; and 3) DAEE increased the expression of RNAs of BDNF and neurotrophin-3 and the protein content of synapse-specific proteins such as synaptophysin, synapsin-1, and syntaxin. Based on these observations we propose that DAEE and some other derivatives of MCFAs having neurotrophin-like neurotrophic activities may become therapeutic tools for certain neurological or psychiatric disorders.
Topics: Animals; Brain-Derived Neurotrophic Factor; Cells, Cultured; Cyclic AMP Response Element-Binding Protein; Embryo, Mammalian; Extracellular Signal-Regulated MAP Kinases; Fatty Acids, Monounsaturated; Hippocampus; Neurons; Neurotrophin 3; Phosphorylation; Rats; Rats, Wistar; Receptors, Nerve Growth Factor; Signal Transduction; Synapses
PubMed: 21187649
DOI: 10.2220/biomedres.31.379