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International Dental Journal Feb 2023The aim of this study was to estimate disease pattern clusters and co-occurrences of oral signs and symptoms in a Colombian population.
OBJECTIVE
The aim of this study was to estimate disease pattern clusters and co-occurrences of oral signs and symptoms in a Colombian population.
METHODS
A cross-sectional study was carried out through a telephone survey amongst 1155 people registered in the telephone directory from Pasto, Colombia. The calls were made from July to November 2019. A 14-item self-report questionnaire about signs and symptoms related to oral diseases that included sociodemographic characteristics was employed. Descriptive and multivariable analyses such as hierarchical clustering, multidimensional scaling, and generalized linear models were used to determine co-occurrences in different sex and age strata.
RESULTS
Age- and condition-specific clusters of signs and symptoms were identified, while sex differences were limited. Calculus and denture sore mouth were related in 18- to 34-year-olds; tooth loss and calculus in 35- to 54-year-olds, and teeth holes or pits (dental caries) and dental abscess in those aged 55 years and older. We found stronger associations between periodontal disease (bleeding gums) and dental caries (odds ratio [OR], 2.484; 95% confidence interval [CI], 1.812-3.405; P < .001) as well as grinding/clenching and facial tension (OR, 7.162; 95% CI, 5.227-9.814; P < .001).
CONCLUSIONS
Age-specific clustering of signs and symptoms and diagnostic patterns wer present in ths Colombian cohort.
Topics: Humans; Male; Female; Dental Caries; Colombia; Cross-Sectional Studies; Mouth Diseases; Periodontal Diseases; Cluster Analysis; Calculi; Oral Health
PubMed: 35851446
DOI: 10.1016/j.identj.2022.06.007 -
American Journal of Physical... Jun 2016Archaeological dental calculus is a rich source of host-associated biomolecules. Importantly, however, dental calculus is more accurately described as a calcified...
OBJECTIVES
Archaeological dental calculus is a rich source of host-associated biomolecules. Importantly, however, dental calculus is more accurately described as a calcified microbial biofilm than a host tissue. As such, concerns regarding destructive analysis of human remains may not apply as strongly to dental calculus, opening the possibility of obtaining human health and ancestry information from dental calculus in cases where destructive analysis of conventional skeletal remains is not permitted. Here we investigate the preservation of human mitochondrial DNA (mtDNA) in archaeological dental calculus and its potential for full mitochondrial genome (mitogenome) reconstruction in maternal lineage ancestry analysis.
MATERIALS AND METHODS
Extracted DNA from six individuals at the 700-year-old Norris Farms #36 cemetery in Illinois was enriched for mtDNA using in-solution capture techniques, followed by Illumina high-throughput sequencing.
RESULTS
Full mitogenomes (7-34×) were successfully reconstructed from dental calculus for all six individuals, including three individuals who had previously tested negative for DNA preservation in bone using conventional PCR techniques. Mitochondrial haplogroup assignments were consistent with previously published findings, and additional comparative analysis of paired dental calculus and dentine from two individuals yielded equivalent haplotype results. All dental calculus samples exhibited damage patterns consistent with ancient DNA, and mitochondrial sequences were estimated to be 92-100% endogenous. DNA polymerase choice was found to impact error rates in downstream sequence analysis, but these effects can be mitigated by greater sequencing depth.
DISCUSSION
Dental calculus is a viable alternative source of human DNA that can be used to reconstruct full mitogenomes from archaeological remains. Am J Phys Anthropol 160:220-228, 2016. © 2016 The Authors American Journal of Physical Anthropology Published by Wiley Periodicals, Inc.
Topics: Anthropology, Physical; Archaeology; DNA, Mitochondrial; Dental Calculus; Genome, Mitochondrial; History, 15th Century; Humans; Sequence Analysis, DNA
PubMed: 26989998
DOI: 10.1002/ajpa.22960 -
Journal (Canadian Dental Association) 2012As implant treatment becomes part of mainstream dental therapy, dental offices should implement protocols for individualized, systematic and continuous supportive care... (Review)
Review
As implant treatment becomes part of mainstream dental therapy, dental offices should implement protocols for individualized, systematic and continuous supportive care of the peri-implant tissues. This review article suggests guidelines for maintenance care of dental implants. The preliminary assessment should begin with updating the patient's medical and dental histories. The clinical implant should be examined to evaluate the following: condition of the soft tissues, plaque index, clinical probing depth, bleeding on probing, suppuration, stability of soft-tissue margins, keratinized tissue, mobility and occlusion. If the clinical signs suggest the presence of peri-implantitis, radiography of the site is advisable, to confirm the diagnosis. Appropriate treatment should be pursued according to any diagnosis reached during the examination, including (but not limited to) instructions on oral hygiene, removal of supra- and sub-gingival plaque and calculus, occlusal adjustment, relining of a removable prosthesis or surgery.
Topics: Clinical Protocols; Dental Care; Dental Implants; Dental Plaque Index; Humans; Medical History Taking; Peri-Implantitis; Periodontal Diseases; Periodontal Index
PubMed: 23306040
DOI: No ID Found -
Brazilian Oral Research Dec 2016Fetuin-A is a potent inhibitor of calcium-phosphate precipitation and of the calcification process, therefore it can also be related with dental calculus. Thus, we aimed...
Fetuin-A is a potent inhibitor of calcium-phosphate precipitation and of the calcification process, therefore it can also be related with dental calculus. Thus, we aimed to investigate a possible relationship between fetuin-A gene polymorphism and the presence of dental calculus. A possible relationship between serum, saliva and gingival crevicular fluid (GCF) levels of fetuin-A was also investigated. Fetuin-A c.742C > T and c.766C > G polymorphisms were investigated in 103 patients with or without dental calculus. Additionally, serum, saliva and GCF fetuin-A levels of patients were compared according to dental calculus presence. A significant difference was not observed in the distribution of the fetuin-A c.742C > T and c.766C > G polymorphisms between patients with or without dental calculus. Saliva and GCF fetuin-A concentrations of patients with dental calculus were statistically higher than those without dental calculus (P=0.001, P=0.036 respectively). According to our results, fetuin-A c.742C > T and c.766C > G polymorphisms were not associated with presence of dental calculus. However, higher GCF and saliva fetuin-A levels were detected in patients with dental calculus than in patients without dental calculus, which may result from an adaptive mechanism to inhibit mineral precipitation and eventually calculus formation.
Topics: Adult; Analysis of Variance; Case-Control Studies; Dental Calculus; Dental Plaque; Enzyme-Linked Immunosorbent Assay; Female; Genetic Association Studies; Genotype; Gingival Crevicular Fluid; Humans; Male; Middle Aged; Polymorphism, Genetic; Reference Values; Saliva; Statistics, Nonparametric; Young Adult; alpha-2-HS-Glycoprotein
PubMed: 28001239
DOI: 10.1590/1807-3107BOR-2016.vol30.0129 -
Bioengineering (Basel, Switzerland) Nov 2023Dental caries on the crown's surface is caused by the interaction of bacteria and carbohydrates, which then gradually alter the tooth's structure. In addition, calculus...
Dental caries on the crown's surface is caused by the interaction of bacteria and carbohydrates, which then gradually alter the tooth's structure. In addition, calculus is the root of periodontal disease. Optical coherence tomography (OCT) has been considered to be a promising tool for identifying dental caries; however, diagnosing dental caries in the early stage still remains challenging. In this study, we proposed an ultrahigh-resolution OCT (UHR-OCT) system with axial and transverse resolutions of 2.6 and 1.8 μm for differentiating the early-stage dental caries and calculus. The same teeth were also scanned by a conventional spectral-domain OCT (SD-OCT) system with an axial resolution of 7 μm. The results indicated that early-stage carious structures such as small cavities can be observed using UHR-OCT; however, the SD-OCT system with a lower resolution had difficulty identifying it. Moreover, the estimated surface roughness and the scattering coefficient of enamel were proposed for quantitatively differentiating the different stages of caries. Furthermore, the thickness of the calculus can be estimated from the UHR-OCT results. The results have demonstrated that UHR-OCT can detect caries and calculus in their early stages, showing that the proposed method for the quantitative evaluation of caries and calculus is potentially promising.
PubMed: 38002442
DOI: 10.3390/bioengineering10111317 -
Quaternary International : the Journal... Apr 2023The study of ancient human dental calculus (mineralized dental plaque, also known as tartar) is becoming increasingly important in osteoarchaeology, human palaeoecology...
The study of ancient human dental calculus (mineralized dental plaque, also known as tartar) is becoming increasingly important in osteoarchaeology, human palaeoecology and environmental archaeology. Microremains of different origin (e.g. starch granules, pollen, phytoliths, feather barbules) as well as biomolecules and chemical compounds retrieved from its mineral matrix may represent an important link between past humans and their physical, biological and social environment, but they are rarely fully linked to the evidence from skeletal remains. This paper critically reviews the lines of evidence retrieved from dental calculus in relation to osteoarchaeological parameters, employing macroscopic, microscopic and biomolecular approaches, assessing synergy potential and limitations. The scope of this paper is also to contribute to the building of a much needed theoretical framework in this emerging subfield.
PubMed: 37089908
DOI: 10.1016/j.quaint.2022.03.003 -
Journal of Clinical Medicine Apr 2023Supervised deep learning requires labelled data. On medical images, data is often labelled inconsistently (e.g., too large) with varying accuracies. We aimed to assess...
Supervised deep learning requires labelled data. On medical images, data is often labelled inconsistently (e.g., too large) with varying accuracies. We aimed to assess the impact of such label noise on dental calculus detection on bitewing radiographs. On 2584 bitewings calculus was accurately labeled using bounding boxes (BBs) and artificially increased and decreased stepwise, resulting in 30 consistently and 9 inconsistently noisy datasets. An object detection network (YOLOv5) was trained on each dataset and evaluated on noisy and accurate test data. Training on accurately labeled data yielded an mAP50: 0.77 (SD: 0.01). When trained on consistently too small BBs model performance significantly decreased on accurate and noisy test data. Model performance trained on consistently too large BBs decreased immediately on accurate test data (e.g., 200% BBs: mAP50: 0.24; SD: 0.05; < 0.05), but only after drastically increasing BBs on noisy test data (e.g., 70,000%: mAP50: 0.75; SD: 0.01; < 0.05). Models trained on inconsistent BB sizes showed a significant decrease of performance when deviating 20% or more from the original when tested on noisy data (mAP50: 0.74; SD: 0.02; < 0.05), or 30% or more when tested on accurate data (mAP50: 0.76; SD: 0.01; < 0.05). In conclusion, accurate predictions need accurate labeled data in the training process. Testing on noisy data may disguise the effects of noisy training data. Researchers should be aware of the relevance of accurately annotated data, especially when testing model performances.
PubMed: 37176499
DOI: 10.3390/jcm12093058 -
Molecular Biology and Evolution Dec 2022Host-associated microbiomes are essential for a multitude of biological processes. Placed at the contact zone between external and internal environments, the...
Host-associated microbiomes are essential for a multitude of biological processes. Placed at the contact zone between external and internal environments, the little-studied oral microbiome has important roles in host physiology and health. Here, we investigate the roles of host evolutionary relationships and ecology in shaping the oral microbiome in three closely related gorilla subspecies (mountain, Grauer's, and western lowland gorillas) using shotgun metagenomics of 46 museum-preserved dental calculus samples. We find that the oral microbiomes of mountain gorillas are functionally and taxonomically distinct from the other two subspecies, despite close evolutionary relationships and geographic proximity with Grauer's gorillas. Grauer's gorillas show intermediate bacterial taxonomic and functional, and dietary profiles. Altitudinal differences in gorilla subspecies ranges appear to explain these patterns, suggesting a close connection between dental calculus microbiomes and the environment, likely mediated through diet. This is further supported by the presence of gorilla subspecies-specific phyllosphere/rhizosphere taxa in the oral microbiome. Mountain gorillas show a high abundance of nitrate-reducing oral taxa, which may promote adaptation to a high-altitude lifestyle by modulating blood pressure. Our results suggest that ecology, rather than evolutionary relationships and geographic distribution, shape the oral microbiome in these closely related species.
Topics: Animals; Gorilla gorilla; Phylogeny; Dental Calculus; Hominidae; Microbiota
PubMed: 36472532
DOI: 10.1093/molbev/msac263 -
Proceedings of the Royal Society of... Oct 1963
Topics: Animals; Dental Calculus; Dental Enamel; Ferritins; Iron; Pigmentation; Research; Rodentia
PubMed: 14068152
DOI: No ID Found -
Animals : An Open Access Journal From... Aug 2021Dental problems are increasingly recognised in cats although many problems are preventable by tooth brushing. However, the knowledge level and preventive measures...
Dental problems are increasingly recognised in cats although many problems are preventable by tooth brushing. However, the knowledge level and preventive measures performed by owners are unclear. Additionally, there is a shortage of information on the communication by veterinary care staff to owners on dental health and prophylaxis in cats. The aim was to describe the knowledge and perceptions among Swedish cat owners and the communication by veterinary care staff on dental problems and prevention in cats. We distributed two electronic surveys; to cat owners and care staff, respectively. Of the cat owners, = 407, 2/3 stated that they have knowledge about dental disease, listing modified eating behaviour as the most common sign followed by gingivitis, halitosis, pain and dental calculus. The main source of information was the internet followed by veterinary care staff, and 47% of the owners reported that they perform preventive oral health measures. The single most frequently stated preventive measure was tooth brushing, which was applied by 28% of the cat owners but with low frequency (daily = 15, every second day = 14). Veterinary care staff, = 179, reported that they inform (47%) or sometimes inform (42%) cat owners on prophylactic dental care, daily or 3-7 times a week (combined 39%) most frequently by oral communication, with tooth brushing as the most common measure recommended. This study suggests that cat owners have relevant knowledge on dental health in cats, but the performed preventive measures are too infrequent to support good oral health in cats. There is room to develop strategies such as improvement in education and communication to increase welfare in cats. This includes consideration of the motivation and practical options for owners of cats who may not tolerate tooth brushing.
PubMed: 34573537
DOI: 10.3390/ani11092571