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Journal of Applied Oral Science :... 2013To evaluate the biocompatibility and the setting time of Portland cement clinker with or without 2% or 5% calcium sulfate and MTA-CPM.
OBJECTIVE
To evaluate the biocompatibility and the setting time of Portland cement clinker with or without 2% or 5% calcium sulfate and MTA-CPM.
MATERIAL AND METHODS
Twenty-four mice (Rattus norvegicus) received subcutaneously polyethylene tubes filled with Portland cement clinker with or without 2% or 5% calcium sulfate and MTA. After 15, 30 and 60 days of implantation, the animals were killed and specimens were prepared for microscopic analysis. For evaluation of the setting time, each material was analyzed using Gilmore needles weighing 113.5 g and 456.5 g, according to the ASTM specification Number C266-08 guideline. Data were analyzed by ANOVA and Tukey's test for setting time and Kruskal-Wallis and Dunn test for biocompatibility at 5% significance level.
RESULTS
Histologic observation showed no statistically significant difference of biocompatibility (p>0.05) among the materials in the subcutaneous tissues. For the setting time, clinker without calcium sulfate showed the shortest initial and final setting times (6.18 s/21.48 s), followed by clinker with 2% calcium sulfate (9.22 s/25.33 s), clinker with 5% calcium sulfate (10.06 s/42.46 s) and MTA (15.01 s/42.46 s).
CONCLUSIONS
All the tested materials showed biocompatibility and the calcium sulfate absence shortened the initial and final setting times of the white Portland cement clinker.
Topics: Aluminum Compounds; Animals; Biocompatible Materials; Calcium Compounds; Calcium Sulfate; Dental Cements; Drug Combinations; Male; Materials Testing; Oxides; Rats; Rats, Wistar; Silicates; Subcutaneous Tissue; Surface Properties; Time Factors
PubMed: 23559109
DOI: 10.1590/1678-7757201302200 -
BMC Oral Health Nov 2023To evaluate the effect of zirconia priming with MDP-Salt before MDP containing primers and self-adhesive cement on the shear bond strength.
PURPOSE
To evaluate the effect of zirconia priming with MDP-Salt before MDP containing primers and self-adhesive cement on the shear bond strength.
MATERIALS AND METHODS
Fully sintered high translucent zirconia specimens (n = 120) were assigned into 2 groups (n = 60 each): Control (No Pretreatment) and Methacryloyloxydecyl dihydrogen phosphate salt (MDP-Salt) pretreated. Each group was divided into 3 subgroups (n = 20) according to cementation protocol: 1) MDP + Silane primer and conventional resin cement, 2) MDP+ Bisphenyl dimethacrylate (BPDM) primer and conventional resin cement, and 3) MDP containing self-adhesive resin cement. Shear bond strength (SBS) was measured after 10,000 thermocycling. Contact angle was measured for tested groups. Surface topography was assessed using a 3D confocal laser scanning microscope (CLSM). Weibull analysis was performed for SBS and one-way ANOVA for contact angle and surface topography measurements (α = 0.05).
RESULTS
The use of MDP-Salt significantly improved the SBS (p < .05) for all tested subgroups. Self-adhesive cement showed an insignificant difference with MDP + Silane group for both groups (p > .05). MDP + BPDM showed a significantly lower characteristic strength compared to self-adhesive resin cement when both were pretreated with MDP-Salt. No difference between all tested groups in the surface topographic measurements while MDP-Salt showed the highest contact angle.
CONCLUSION
MDP-Salt pretreatment can improve bonding performance between zirconia and MDP containing products.
Topics: Humans; Resin Cements; Cementation; Dental Cements; Salts; Dental Bonding; Silanes; Methacrylates; Shear Strength; Materials Testing; Surface Properties; Dental Stress Analysis
PubMed: 37993834
DOI: 10.1186/s12903-023-03663-y -
Indian Journal of Dental Research :... 2010To assess the influence of silane evaporation procedures on bond strength between a dental ceramic and a chemically activated resin cement.
AIM
To assess the influence of silane evaporation procedures on bond strength between a dental ceramic and a chemically activated resin cement.
MATERIALS AND METHODS
Eighteen blocks (6 mm Chi 14 mm Chi 14 mm) of ceramic IPS Empress 2 were cemented (C and B) to composite resin (InTen-S) blocks using a chemical adhesive system (Lok). Six groups were analyzed, each with three blocks divided according to ceramic surface treatment: two control groups (no treatment, NT; 10% hydrofluoric acid plus silane Monobond-S dried at room temperature, HFS); the other four groups comprised different evaporation patterns (silane rinsed and dried at room temperature, SRT; silane rinsed in boiling water and dried as before, SBRT; silane rinsed with boiling water and heat dried at 50 degrees C, SBH; silane dried at 50 +/- 5 degrees C, rinsed in boiling water and dried at room temperature, SHBRT). The cemented blocks were sectioned to obtain specimens for microtensile test 7 days after cementation and were stored in water for 30 days prior to testing. Fracture patterns were analyzed by optical and scanning electron microscopy. Statistics and
RESULTS
All blocks of NT debonded during sectioning. One way ANOVA tests showed higher bond strengths for HFS than for the other groups. SBRT and SBH were statistically similar, with higher bond strengths than SRT and SHBRT. Failures were 100% adhesive in SRT and SHBRT. Cohesive failures within the "adhesive zone" were detected in HFS (30%), SBRT (24%) and SBH (40%).
CONCLUSION
Silane treatment enhanced bond strength in all conditions evaluated, showing best results with HF etching.
Topics: Bisphenol A-Glycidyl Methacrylate; Dental Bonding; Dental Cements; Dental Etching; Dental Porcelain; Dental Stress Analysis; Hydrofluoric Acid; Lithium Compounds; Microscopy, Electron, Scanning; Resin Cements; Self-Curing of Dental Resins; Silanes; Surface Properties; Tensile Strength; Volatilization
PubMed: 20657094
DOI: 10.4103/0970-9290.66645 -
European Journal of Oral Sciences Oct 2022Glass ionomer (GI) cements and self-etch (SE) or universal adhesives after etching (ER) adapt variably with dentine. Dentine characteristics vary with depth...
Glass ionomer (GI) cements and self-etch (SE) or universal adhesives after etching (ER) adapt variably with dentine. Dentine characteristics vary with depth (deep/shallow), location (central/peripheral), and microscopic site (intertubular/peritubular). To directly compare adhesion to dentine, non-destructive imaging and testing are required. Here, GI, ER, and SE adapted at different dentine depths, locations, and sites were investigated using micro-CT, xenon plasma focused ion beam scanning electron microscopy (Xe PFIB-SEM), and energy dispersive X-ray spectroscopy (EDS). Extracted molars were prepared to deep or shallow slices and treated with the three adhesives. Micro-CT was used to compare changes to air volume gaps, following thermocycling, and statistically analysed using a quantile regression model and Fisher's exact test. The three adhesives performed similarly across dentine depths and locations, yet no change or overall increases and decreases in gaps at all dentine depths and locations were measured. The Xe PFIB-SEM-milled dentine-adhesive interfaces facilitated high-resolution characterization, and element profiling revealed variations across the tooth-material interfaces. Dentine depth and location had no impact on adhesive durability, although microscopic differences were observed. Here we demonstrate how micro-CT and Xe PFIB-SEM can be used to compare variable dental materials without complex multi-stage specimen preparation to minimize artefacts.
Topics: Dental Bonding; Dental Cements; Dental Materials; Dentin; Dentin-Bonding Agents; Glass Ionomer Cements; Materials Testing; Microscopy, Electron; Microscopy, Electron, Scanning; Resin Cements; Surface Properties; X-Rays; Xenon
PubMed: 35959863
DOI: 10.1111/eos.12890 -
Dental Materials Journal Jun 2020Copper nanoparticles (NCu) were synthetized and added to commercial glass ionomer cement, to evaluate in vitro its antibacterial activity against oral cavity strains....
Copper nanoparticles (NCu) were synthetized and added to commercial glass ionomer cement, to evaluate in vitro its antibacterial activity against oral cavity strains. The NCu were synthesized by copper acetate reduction with L-ascorbic acid and characterized by FTIR, Raman, XPS, XRD and TEM. Then, commercial glass ionomer cement (GIC) was modified (MGIC) with various concentrations of NCu and physicochemically characterized. Cell viability was tested against human dental pulp fibroblasts (HDPFs) by Alamar-Blue assay and antibacterial test was performed against S. mutans and S. sanguinis by colony forming unit (CFU) growth method. Synthesized NCu rendered a mixture of both metallic copper and cuprous oxide (CuO). HDPF viability reduces with exposure time to the extracts (68-72% viability) and MGIC with 2-4 wt% NCu showed antimicrobial activity against the two tested strains.
Topics: Anti-Bacterial Agents; Copper; Glass Ionomer Cements; Humans; Materials Testing; Nanoparticles; Streptococcus mutans
PubMed: 32213765
DOI: 10.4012/dmj.2019-046 -
Dental Materials Journal Aug 2023To improve the cytocompatibility of mineral trioxide aggregate (MTA) cement and its ability for reparative dentin formation, the effect of adding choline dihydrogen...
To improve the cytocompatibility of mineral trioxide aggregate (MTA) cement and its ability for reparative dentin formation, the effect of adding choline dihydrogen phosphate (CDHP), which is reported to be biocompatible, to MTA cement was investigated. The L929 cell proliferation showed that the addition of CDHP improved cell viability. The addition of CDHP shortened the setting time of MTA cement, with a significant decrease in consistency above 0.4 g/mL. Diametral tensile strength of the set cement was improved by the addition of 0.4 g/mL CDHP. Solubility was judged to be within the range of clinical application. The spontaneous precipitation of low crystalline hydroxyapatite was examined by immersing the set cement in phosphate buffer saline, and it was found that the ability of the cement with 0.4 g/mL of CDHP was significantly improved compared with that of the cement without CDHP.
Topics: Materials Testing; Root Canal Filling Materials; Calcium Compounds; Oxides; Dental Cements; Silicates; Glass Ionomer Cements; Aluminum Compounds; Drug Combinations; Phosphates; Choline
PubMed: 37121734
DOI: 10.4012/dmj.2022-283 -
Dental Materials Journal 2015Influences of contamination and cleaning methods on the bonding of resin cement to zirconia ceramics were examined. Airborne particle-abraded zirconia (IPS e.max ZirCAD)...
Influences of contamination and cleaning methods on the bonding of resin cement to zirconia ceramics were examined. Airborne particle-abraded zirconia (IPS e.max ZirCAD) specimens were contaminated with saliva and cleaned with tap water (SC) or by application of 37% phosphoric acid (PA), Ivoclean (IC), or additional airborne particle abrasion (AB). Specimens without contamination served as controls. After application of Monobond Plus to the surface of the specimens, resin cement was mixed and inserted into a mold. Surface free energies of the specimens were determined by measuring contact angles. Surface treatment and storage conditions significantly influenced bond strength, while there was no significant interaction between the two factors. Surface free energies of the SC and IC groups were significantly lower than those of the other groups. Additional AB of saliva-contaminated zirconia increased the strength of bonding with the resin cement as well as increased surface free energy.
Topics: Air Abrasion, Dental; Decontamination; Dental Bonding; Dental Cements; In Vitro Techniques; Materials Testing; Phosphoric Acids; Resin Cements; Saliva; Surface Properties; Water; Zirconium
PubMed: 25748464
DOI: 10.4012/dmj.2014-066 -
Journal of Indian Prosthodontic Society 2021To evaluate the microleakage of metallic copings luted with three different commercially available luting cements.
AIM
To evaluate the microleakage of metallic copings luted with three different commercially available luting cements.
SETTING AND DESIGN
Comparative evaluation in vitro study.
MATERIALS AND METHODS
Thirty replicas of abutment analog were milled and divided into 3 groups. Nickel chromium copings were fabricated; marginal gap was evaluated with optical microscope and luted with Zinc Oxide Non Eugenol cement, Zinc Polycarboxylate cement and Zinc Phosphate cement. After Thermocycling, cemented specimens were placed into 0.5% aqueous solution of basic fuchsin solution for 24 hours for dye penetration. Copings were longitudinally sectioned and microleakage scores were evaluated.
STATISTICAL ANALYSIS USED
Kruskal-Wallis analysis of variance (ANOVA) followed by Chi-Square test. Pairwise comparison of groups with Mann Whitney U test.
RESULT
Mean microleakage score was least for Zinc Phosphate cement (1.075 ± 0.85) followed by Zinc Polycarboxylate cement (1.80± 0.23) and most for Zinc Oxide Non Eugenol (2.1± 0.37). The results of the study were statistically significant, P < 0.05.
CONCLUSIONS
Within the limitations of the study, it was found that all cements exhibited certain amount of microleakage. Zinc Phosphate cement exhibited a mean microleakage score that was significantly lower than Zinc Oxide Non Eugenol cement and Zinc Polycarboxylate cement. When microleakage scores of Zinc Oxide Non Eugenol cement and Zinc Polycarboxylate cement were compared, the difference was found to be insignificant indicating that microleakage in these cements is similar.
Topics: Cementation; Glass Ionomer Cements; Polycarboxylate Cement; Resin Cements; Zinc Phosphate Cement
PubMed: 33835069
DOI: 10.4103/jips.jips_19_20 -
BMC Oral Health Nov 2023This study aimed to investigate different surface treatments thought to increase the bond strength between zirconia ceramic and adhesive resin cement.
BACKGROUND
This study aimed to investigate different surface treatments thought to increase the bond strength between zirconia ceramic and adhesive resin cement.
METHODS
The samples were prepared in 15 × 10 × 2 mm dimensions by cutting off monolithic zirconia ceramic blocks (Incoris TZI; Sirona, Germany). Surface roughness measurements were made with a profilometer, the average surface roughness (Ra1) was recorded, and five different surface treatments were applied. Group 1: Control group. No surface treatment was applied. Group 2: Sandblasted with AlO under pressure of 50 μm. Group 3: Sandblasted with 30 μm AlO - SiOx under pressure, then tribochemical silica coating, silane bonding agent, and ceramic primer were applied. Group 4: Samples were etched in a hot acid solution containing methanol, HCl, and chloride at 100 °C. Group 5: Samples were coated in a solution containing Grade C Aluminum Nitrite at 75 °C for 15 Sects. 12,000 thermal aging was carried out to all samples. Then, samples were bonded to a composite surface (Filtek Z250) with two different types of adhesive cement (Panavia F 2.0, Rely X U200) (n = 10). A load was applied to the samples attached to the Universal Test Device for the SBS, and the SBS was recorded. The surface roughness measurements of all samples were made again, and the average surface roughness Ra2 was recorded. The data was analyzed with a two-way ANOVA test. Bonferroni correction was used for multiple comparisons of the groups. p = 0.005 was accepted as the statistically significant value.
RESULTS
There was no statistically significant difference between the groups in the Ra1 measurements (p = 0.031). There was a statistically significant difference between the Ra2 values of Groups 4 and 5 and the Ra2 values of Groups 1,2 and 3 in the Ra2 measurements (p < 0.001). There was no statistically significant difference between the SBS values of the groups (p > 0.005). Also, there was no statistically significant difference in the SBS values of all groups for the two different cements tested (p > 0.005).
CONCLUSIONS
None of the surface treatments applied to monolithic zirconia ceramic samples increased the SBS between ceramic and adhesive resin cement.
Topics: Humans; Resin Cements; Dental Cements; Dental Bonding; Shear Strength; Ceramics; Surface Properties; Materials Testing; Dental Stress Analysis
PubMed: 37957657
DOI: 10.1186/s12903-023-03630-7 -
Journal of Materials Science. Materials... Jan 2021Adhesive resin-cements are increasingly used in modern dentistry. Nevertheless, released substances from resin materials have been shown to cause cellular toxic effects....
Adhesive resin-cements are increasingly used in modern dentistry. Nevertheless, released substances from resin materials have been shown to cause cellular toxic effects. Disc-shaped specimens from 12 different resin cements and one conventional zinc phosphate cement were prepared and used for direct stimulation of five different human cell lines via transwell cell culture system or in an indirect way using conditioned cell culture media. Cytotoxicity was determined using LDH and BCA assays. All tested cements led to a decrease of cell viability but to a distinct extent depending on cell type, luting material, and cytotoxicity assay. In general, cements exhibited a more pronounced cytotoxicity in direct stimulation experiments compared to stimulations using conditioned media. Interestingly, the conventional zinc phosphate cement showed the lowest impact on cell viability. On cellular level, highest cytotoxic effects were detected in osteoblastic cell lines. All resin cements reduced cell viability of human cells with significant differences depending on cell type and cement material. Especially, osteoblastic cells demonstrated a tremendous increase of cytotoxicity after cement exposure. Although the results of this in vitro study cannot be transferred directly to a clinical setting, it shows that eluted substances from resin cements may disturb osteoblastic homeostasis that in turn could lead to conditions favoring peri-implant bone destruction. Thus, the wide use of resin cements in every clinical situation should be scrutinized. A correct use with complete removal of all cement residues and a sufficient polymerization should be given the utmost attention in clinical usage.
Topics: A549 Cells; Cell Line; Cell Survival; Culture Media, Conditioned; Dental Cements; Glass Ionomer Cements; Humans; In Vitro Techniques; Keratinocytes; L-Lactate Dehydrogenase; Materials Testing; Osteoblasts; Phosphates; Polymerization; Resin Cements; Resins, Synthetic; Zinc Compounds; Zinc Phosphate Cement
PubMed: 33471194
DOI: 10.1007/s10856-020-06471-w