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The Biochemical Journal Oct 1970
Topics: Centrifugation, Density Gradient; Chromatography; DNA; Microscopy, Electron; Mitochondria; Molecular Weight; Tetrahymena
PubMed: 5492839
DOI: 10.1042/bj1190061pb -
Journal of Virology Jan 1971The products of the deoxyribonucleic acid (DNA) polymerase associated with Rous sarcoma virus and avian myeloblastosis virus were characterized by correlative analyses...
The products of the deoxyribonucleic acid (DNA) polymerase associated with Rous sarcoma virus and avian myeloblastosis virus were characterized by correlative analyses with equilibrium centrifugation and stepwise elution from hydroxyapatite. The initial enzymatic product consists of nascent DNA chains which are hydrogen-bonded to 70S viral ribonucleic acid (RNA), whereas the final enzymatic product is double-stranded DNA. Appreciable amounts of free single-stranded DNA were not detected at any point during the course of the enzymatic reaction, but the data in this regard are not decisive. The time course of synthesis of DNA:RNA hybrids and double-stranded DNA has been analyzed. It is concluded that the synthesis of double-stranded DNA is a sequel to and is probably dependent upon the synthesis of DNA:RNA hybrid.
Topics: Animals; Avian Leukosis Virus; Cattle; Centrifugation, Density Gradient; Centrifugation, Zonal; Cesium; Chick Embryo; Chromatography; DNA; DNA Nucleotidyltransferases
PubMed: 4322606
DOI: 10.1128/JVI.7.1.77-86.1971 -
Chemical Research in Toxicology Mar 2019Understanding the toxicological implications of deoxyribonucleic acid (DNA) oxidation arising from cellular oxidative stress depends on identifying DNA oxidation...
Understanding the toxicological implications of deoxyribonucleic acid (DNA) oxidation arising from cellular oxidative stress depends on identifying DNA oxidation products, their location in the genome, and their interaction with repair, replication, and gene expression.
Topics: DNA; Genomics; Oxidation-Reduction; Oxidative Stress
PubMed: 30807111
DOI: 10.1021/acs.chemrestox.9b00046 -
Infection and Immunity Jun 1979The fate of lymphogranuloma venereum strain Chlamydia-infected HeLa 229 cells was examined by determining the rate of deoxyribonucleic acid synthesis and the kinetics of...
The fate of lymphogranuloma venereum strain Chlamydia-infected HeLa 229 cells was examined by determining the rate of deoxyribonucleic acid synthesis and the kinetics of entry into and progression through S phase and by time-lapse cinemicrography. At an input multiplicity of 5 or less, Chlamydia-infected cells showed no inhibition of host deoxyribonucleic acid synthesis or cell cycle progression. Cinemicrography showed division of inclusion-containing cells, with one or both daughters receiving chlamydial inclusions. Analysis of the family trees indicated that the generation times of infected HeLa 229 were not altered relative to those of the uninfected cells.
Topics: Cell Cycle; Cell Division; Chlamydia trachomatis; DNA; HeLa Cells; Inclusion Bodies, Viral; Kinetics
PubMed: 468381
DOI: 10.1128/iai.24.3.953-957.1979 -
The Journal of Biophysical and... Nov 1961Microspores isolated from Lilium longiflorum and Trillium erectum were studied with respect to their capacities for phosphorylating deoxyribosides in vitro. It was found...
Microspores isolated from Lilium longiflorum and Trillium erectum were studied with respect to their capacities for phosphorylating deoxyribosides in vitro. It was found that such capacities are manifest only during brief intervals of time adjacent to periods of DNA synthesis, and that none of the neighboring cells in the anther acquire them. The observed patterns of behavior are interpreted in terms of enzyme induction as a device for regulating DNA synthesis.
Topics: DNA; Lilium; Phosphorylases; Phosphorylation; Trillium
PubMed: 14449225
DOI: 10.1083/jcb.11.2.311 -
Indian Journal of Ophthalmology Feb 2023This study aimed to assess the severity of deoxyribonucleic acid (DNA) damage in lens epithelial cells (LECs) of senile cortical, nuclear, and posterior subcapsular...
PURPOSE
This study aimed to assess the severity of deoxyribonucleic acid (DNA) damage in lens epithelial cells (LECs) of senile cortical, nuclear, and posterior subcapsular cataracts.
METHODS
LECs were obtained from senile cortical, nuclear, and subcapsular types of cataracts after surgery. DNA damage in the cells was immediately assessed quantitatively using the CometScore software.
RESULTS
Comets were found in cataractous LECs. The formation of "comets" in the DNA of LECs can be visualized using single-cell gel electrophoresis and indicates DNA strand breaks because the damaged DNA migrates at a different rate than the nondamaged DNA. Maximal damage was observed in Grade 3 cortical, nuclear, and subcapsular forms of cataracts. Statistically significant DNA damage was seen between grades 1 and 3 of cortical type of cataract, grades 1 and 3 of nuclear type of cataract, and grades 2 and 3 and grades 1 and 3 of posterior subcapsular type of cataract.
CONCLUSION
In patients with senile cataract, DNA of LECs was randomly damaged, and this type of damage was possibly caused by reactive oxygen species (ROS). Maximum DNA damage was found in patients with Grade 3 senile cortical, nuclear, and subcapsular type cataracts. The pathogenesis of senile cataracts is multifactorial and includes continuous molecular stress resulting from photooxidative stress, UV irradiation, and oxidative reactions.
Topics: Humans; Cataract; Aging; Reactive Oxygen Species; DNA; Epithelial Cells; Lens, Crystalline
PubMed: 36727354
DOI: 10.4103/ijo.IJO_1730_22 -
The Journal of Biological Chemistry Aug 1956
Topics: DNA; DNA Replication; Digestion; Liver
PubMed: 13357466
DOI: No ID Found -
Journal of Bacteriology Sep 1962Firshein, William (Wesleyan University, Middletown, Conn.). Effect of manganese and enzymatic deoxyribonucleic acid digests on population changes and respiration of...
Firshein, William (Wesleyan University, Middletown, Conn.). Effect of manganese and enzymatic deoxyribonucleic acid digests on population changes and respiration of pneumococci. J. Bacteriol. 84:478-484. 1962.-Deoxyribonucleic acid (DNA; Na salt) treated with deoxyribonuclease and Mn(++) (as MnSO(4)) stimulated R (avirulent) to S (virulent) population changes in vitro. Under certain environmental conditions and with certain strains, Mn(++) alone elicited these changes, but, in most cases, both digested DNA and Mn(++) were necessary for maximal effect. These population changes were due to a selective stimulation of the multiplication of S cells and a delay in S-cell lysis, with either a slight inhibitory effect or no effect against the multiplication of R cells. The magnitude of the population change depended upon the presence of digested DNA, the level of Mn(++), the strain, and the concentration of basal medium. Mn(++) enhanced respiration of S cells while inhibiting that of R cells.
Topics: DNA; Deoxyribonucleases; Ions; Manganese; Streptococcus pneumoniae
PubMed: 13945241
DOI: 10.1128/jb.84.3.478-484.1962 -
Scientific Reports Mar 2024The present research investigates the double-chain deoxyribonucleic acid model, which is important for the transfer and retention of genetic material in biological...
The present research investigates the double-chain deoxyribonucleic acid model, which is important for the transfer and retention of genetic material in biological domains. This model is composed of two lengthy uniformly elastic filaments, that stand in for a pair of polynucleotide chains of the deoxyribonucleic acid molecule joined by hydrogen bonds among the bottom combination, demonstrating the hydrogen bonds formed within the chain's base pairs. The modified extended Fan sub equation method effectively used to explain the exact travelling wave solutions for the double-chain deoxyribonucleic acid model. Compared to the earlier, now in use methods, the previously described modified extended Fan sub equation method provide more innovative, comprehensive solutions and are relatively straightforward to implement. This method transforms a non-linear partial differential equation into an ODE by using a travelling wave transformation. Additionally, the study yields both single and mixed non-degenerate Jacobi elliptic function type solutions. The complexiton, kink wave, dark or anti-bell, V, anti-Z and singular wave shapes soliton solutions are a few of the creative solutions that have been constructed utilizing modified extended Fan sub equation method that can offer details on the transversal and longitudinal moves inside the DNA helix by freely chosen parameters. Solitons propagate at a consistent rate and retain their original shape. They are widely used in nonlinear models and can be found everywhere in nature. To help in understanding the physical significance of the double-chain deoxyribonucleic acid model, several solutions are shown with graphics in the form of contour, 2D and 3D graphs using computer software Mathematica 13.2. All of the requisite constraint factors that are required for the completed solutions to exist appear to be met. Therefore, our method of strengthening symbolic computations offers a powerful and effective mathematical tool for resolving various moderate nonlinear wave problems. The findings demonstrate the system's potentially very rich precise wave forms with biological significance. The fundamentals of double-chain deoxyribonucleic acid model diffusion and processing are demonstrated by this work, which marks a substantial development in our knowledge of double-chain deoxyribonucleic acid model movements.
Topics: Nonlinear Dynamics; Base Pairing; Biological Science Disciplines; Hydrogen Bonding; DNA
PubMed: 38494490
DOI: 10.1038/s41598-024-55786-z -
Journal of Bacteriology May 1966Anderson, D. L. (University of Minnesota, Minneapolis), D. D. Hickman, and B. E. Reilly. Structure of Bacillus subtilis bacteriophage phi29 and the length of phi29...
Anderson, D. L. (University of Minnesota, Minneapolis), D. D. Hickman, and B. E. Reilly. Structure of Bacillus subtilis bacteriophage phi29 and the length of phi29 deoxyribonucleic acid. J. Bacteriol. 91:2081-2089. 1966-Bacillus subtilis bacteriophage phi29 were negatively stained with phosphotungstic acid. The head of phi29 has a hexagonal outline with a flattened base, and is about 315 A wide and 415 A in length. The virus has an intricate tail about 325 A in length. Twelve spindle-shaped appendages are attached to the lower of two collars which comprise the proximal portion of the tail. The distal 130 A of the tail axis has a diameter of about 60 A and is larger in diameter than the axis of the upper portion of the tail. Comparison of electron microscopic counts of phi29 with plaque-forming units indicated that about 50% of the microscopic entities were infective. Phenol-extracted phi29 deoxyribonucleic acid (DNA) molecules were prepared for electron microscopy by the cytochrome c film technique of Kleinschmidt et al. Measurement of contour lengths of DNA molecules from three preparations gave skewed distributions of lengths with observed modal class values ranging from 5.7 to 5.9 mu. Assuming that phi29 DNA is a double helix in the B form, the corresponding molecular weights would be 10.9 x 10(6) to 11.3 x 10(6) daltons. The largest DNA molecules would have a volume of 1.9 x 10(7) A(3) which is about 25% greater than the estimated 1.4 x 10(7) A(3) internal volume of the phage head.
Topics: Bacillus subtilis; Bacteriophages; DNA, Viral; In Vitro Techniques; Microscopy, Electron; Molecular Weight
PubMed: 4957028
DOI: 10.1128/jb.91.5.2081-2089.1966