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Journal of Bacteriology Nov 1979Plasmid and phage deoxyribonucleic acid (DNA) harboring bacterial insertion sequence (IS) elements IS1, IS2, and IS5 were characterized and used as probes to detect...
Plasmid and phage deoxyribonucleic acid (DNA) harboring bacterial insertion sequence (IS) elements IS1, IS2, and IS5 were characterized and used as probes to detect homologous sequences in various procaryotic and eucaryotic genomes. The hybridization method used permits the detection of sequences partially homologous to the elements. Hybridization of the IS-containing probes to each other revealed a region of limited homology between IS1 and IS2. Homologous sequences were then detected by computer analysis of the published IS1 and IS2 nucleotide sequences. The homologous sequence contains a tandemly repeated tetranucleotide sequence which resembles the repeated sequence at the hot spot for spontaneous mutations in the lacI gene (P. J. Farabaugh, U. Schmeissner, M. Hofer, and J. Miller, J. Mol. Biol. 126:847-863, 1978). Homology between the IS elements and various genomes was determined by hybridizing labeled DNA containing IS1, IS2, and IS5 sequences to Southern blots of chromosomal DNA cleaved with restriction endonucleases. IS1 and IS5 appear limited to the enteric bacteria, whereas IS2 sequences can also be detected in Pseudomonas putida, Pseudomonas aeruginosa, and Serratia marcescens. Bacteria which appear not to possess extrachromosomal elements, e.g., Caulobacter crescentus, did not show homology with any insertion sequences tested. In addition, sequences homologous to IS1, IS2, or IS5 were not detected in Saccharomyces cerevisiae, Dictyostelium discoideum, or calf thymus DNA.
Topics: Bacteria; Bacteriophage lambda; Base Sequence; Computers; DNA; DNA Transposable Elements; DNA, Bacterial; DNA, Fungal; Nucleic Acid Hybridization; Plasmids
PubMed: 159291
DOI: 10.1128/jb.140.2.588-596.1979 -
Journal of Dairy Science Oct 1985Growth of the mammary gland is measured by several indices including total wet weight, dry fat-free tissue, and deoxyribonucleic acid. The latter is a superior measure... (Comparative Study)
Comparative Study
Growth of the mammary gland is measured by several indices including total wet weight, dry fat-free tissue, and deoxyribonucleic acid. The latter is a superior measure of true growth because it represents changes of cell numbers. Sufficient data have been generated to determine the relationship among species of mammals between gestation length and differences in rates of mammary growth. Exponential growth equations were estimated for eight mammalian species with gestation lengths from 16.5 d for the hamster to 280 d for the cow. The form of the most appropriate equation was Y = AeBx, where Y is mammary deoxyribonucleic acid or dry fat-free tissue, x is day of gestation, e is the base of natural logs, and A and B are constants. The A term was related to body weight (W) and the B-term to gestation length (G). Resulting equations were deoxyribonucleic acid (mg) = .0547W.803 e1.98 G-.98x and dry fat-free tissue (mg) = 2.35W.779 e.719 G-.77x. First-order rate constants of mammary growth ranged in a reverse order from a high of .141 d-1 in hamsters to a low of .008 d-1 in cows; in other words, mammary deoxyribonucleic acid in hamsters doubled in 4.9 d but in the bovine it took 87 d to double.
Topics: Animals; Cattle; Cricetinae; DNA; Female; Gestational Age; Goats; Guinea Pigs; Mammary Glands, Animal; Mice; Pregnancy; Rabbits; Rats; Sheep; Species Specificity
PubMed: 4067035
DOI: 10.3168/jds.S0022-0302(85)81139-5 -
Proceedings of the National Academy of... Feb 1964
Topics: DNA; DNA Replication; DNA, Bacterial; Escherichia coli; Metabolism; Nucleotides; Oligonucleotides; Research; Spectrophotometry; Temperature
PubMed: 14124330
DOI: 10.1073/pnas.51.2.315 -
The Journal of Experimental Medicine May 1958Spherical particles, 1 to 10 microns in diameter, resulted from the incubation at 37 degrees C. of distilled water lysates of erythrocytes with deoxyribonucleic acid...
Spherical particles, 1 to 10 microns in diameter, resulted from the incubation at 37 degrees C. of distilled water lysates of erythrocytes with deoxyribonucleic acid (DNA). The particles consisted of 88 per cent hemoglobin and 12 per cent DNA (dry weight basis). An unknown factor, presumably an enzyme, present only in fresh red cell lysates, was required for particle development. Particle size was a function of the pH of the reaction mixture. The pH range was 4.8-5.8. It was possible to trap extraneous proteins and polysaccharides in pockets within the hemoglobin particles during their development to the exclusion of some of the hemoglobin. The amount of any one substance so trapped was proportional to its concentration in the reaction mixture. Some practical applications of the particles, as a means for making particulate various soluble substances (enzymes, antigens, antibiotics), are suggested.
Topics: Cytoplasm; DNA; Erythrocytes; Hemoglobins; In Vitro Techniques; Water
PubMed: 13525584
DOI: 10.1084/jem.107.5.769 -
Journal of Bacteriology Aug 1974Labeled ribonucleic acid (RNA) complementary to Agrobacterium tumefaciens DNA and PS8 bacteriophage DNA (cRNA) were used in a systematic study of the sensitivity of...
Attempts to detect deoxyribonucleic acid from Agrobacterium tumefaciens and bacteriophage PS8 in crown gall tumors by complementary ribonucleic acid-deoxyribonucleic acid-filter hybridization.
Labeled ribonucleic acid (RNA) complementary to Agrobacterium tumefaciens DNA and PS8 bacteriophage DNA (cRNA) were used in a systematic study of the sensitivity of cRNA/deoxyribonucleic acid (DNA)-filter hybridization for detection of small amounts of phage or bacterial DNA immobilized on filters. A. tumefaciens cRNA of specific activity 10(6) to 2 x 10(6) counts per min per mug reacted to a significant extent when the DNA-filter contained 1% A. tumefaciens DNA in a salmon DNA background, but 0.1% A. tumefaciens DNA was not detectable. PS8 phage cRNA of the same specific activity reacted to a significant extent when the DNA-filter contained as little as 0.01% PS8 DNA in a salmon DNA background. Both kinds of cRNA were found to bind to tobacco crown gall tumor DNA-filters. Similar reaction was found with control normal callus DNA-filters but not with tobacco seedling DNA-filters. The "hybrids" formed by cRNA with normal callus and tumor DNA-filters had low thermal stability. Attempts to purify the tumor and normal callus DNA prior to immobilization on the filter resulted in elimination of this spurious binding. No evidence was found for bacterial or phage DNA in crown gall tumor DNA.
Topics: Bacteriophages; DNA Viruses; DNA, Bacterial; DNA, Viral; Methods; Nucleic Acid Hybridization; Plant Diseases; Plants; RNA, Bacterial; Rhizobium; Temperature; Tritium; Ultrafiltration
PubMed: 4850689
DOI: 10.1128/jb.119.2.547-553.1974 -
Journal of Bacteriology Dec 1980A nick-labeling method has been used to localize the relaxation complex nick sites in three plasmids (pSC101, RSF1010, and R6K) that differ markedly in their host range,...
Location of two relaxation nick sites in R6K and single sites in pSC101 and RSF1010 close to origins of vegetative replication: implication for conjugal transfer of plasmid deoxyribonucleic acid.
A nick-labeling method has been used to localize the relaxation complex nick sites in three plasmids (pSC101, RSF1010, and R6K) that differ markedly in their host range, deoxyribonucleic acid replication, and conjugal transfer properties. Single specific relaxation sites were located in pSC101 and RSF1010, but surprisingly two distinct sites could be identified in the bi-origin plasmid R6K. In all cases, relaxation nick sites, which are thought to be origins of plasmid conjugal transfer, were shown to be located near origins of vegetative replication. This result suggests a functional interaction between these two types of deoxyribonucleic acid loci, and we speculate here that application events initiated at origins of replication may constitute an integral part of the process of conjugal transfer of small plasmids among bacteria. Consistent with this proposal is the finding that inhibition of vegetative replication of the pSC101 and ColE1 plasmids results in a severe inhibition of their conjugal transfer ability.
Topics: Conjugation, Genetic; DNA Replication; DNA Restriction Enzymes; DNA, Bacterial; Escherichia coli; Models, Genetic; Plasmids
PubMed: 6254952
DOI: 10.1128/jb.144.3.923-932.1980 -
Journal of Dairy Science Mar 1981Nine groups of five dairy goats from four breeds, with each breed represented at least once in each group, were in our experiment to measure mammary gland growth. Groups...
Nine groups of five dairy goats from four breeds, with each breed represented at least once in each group, were in our experiment to measure mammary gland growth. Groups were virgins, days 90 to 100 pregnancy, 5 days prepartum (145 days pregnancy), 1 or 2 days prepartum, and 1 or 2, 5, 10, 15, and 30 days in lactation. Virgins were 19 mo old while all others had one pregnancy and lactation prior to experiment. Indices of mammary growth included untrimmed and trimmed wet weights of udders, dried fat-free tissue weights, deoxyribonucleic acid and ribonucleic acid contents. Each index was in a regression equation best to describe the pattern of mammary growth. These included linear, quadratic, cubic, and exponential model Y = AebX in which Y was the index of mammary growth and X was the day of pregnancy. Total deoxyribonucleic acid was the best index of mammary growth with a correlation of .95 in the exponential model. The predictive equation for total deoxyribonucleic acid in milligram was Y = 167e.019X on a daily basis, and the rate of growth on a monthly basis was .57. Mammary growth in goats continued into early lactation, peaking at day 5. Ribonucleic acid doubled on the day after parturition, which reflected the rapid increase in protein synthesis at this time.
Topics: Animals; DNA; Female; Goats; Lactation; Mammary Glands, Animal; Pregnancy; Pregnancy, Animal; RNA
PubMed: 6167600
DOI: 10.3168/jds.S0022-0302(81)82589-1 -
The Journal of Biological Chemistry Jan 1964
Topics: Alkaline Phosphatase; Chromatography; DNA; DNA, Bacterial; Escherichia coli; Exonucleases; Phosphoric Monoester Hydrolases; Phosphorus Isotopes; Research
PubMed: 14114850
DOI: No ID Found -
Journal of the American College of... Apr 2014
Topics: DNA; Genotype; Graft Rejection; Heart Transplantation; Humans; Predictive Value of Tests; Prospective Studies; Sensitivity and Specificity; Single-Blind Method; Tissue Donors
PubMed: 24140666
DOI: 10.1016/j.jacc.2013.09.029 -
The Journal of Biological Chemistry May 1972
Topics: Adenosine Triphosphate; Alkaline Phosphatase; Catalysis; Chromatography, Gel; Cytosine Nucleotides; DNA Nucleotidyltransferases; DNA Repair; DNA, Bacterial; DNA, Single-Stranded; Deoxyribonucleases; Deoxyribonucleotides; Drug Stability; Escherichia coli; Hot Temperature; Isotope Labeling; Micrococcal Nuclease; Nucleotides; Phosphoric Diester Hydrolases; Phosphorus Isotopes; Polynucleotides; Templates, Genetic; Thymine; Tritium
PubMed: 4337512
DOI: No ID Found