-
Bioscience, Biotechnology, and... Oct 2000Virgin females of M. confusa, A. peponis, and C. eriosoma secrete (Z)-7-dodecenyl acetate as a common main pheromone component. Their pheromone titers decreased after...
Virgin females of M. confusa, A. peponis, and C. eriosoma secrete (Z)-7-dodecenyl acetate as a common main pheromone component. Their pheromone titers decreased after decapitation, and increased in the decapitated females after injection of a synthetic hormone, pheromone biosynthetic activating neuropeptide (PBAN) of Bombyx mori. In addition, an extract of brain-subesophageal ganglion complexes of each Plusiinae species activated pheromone biosynthesis in decapitated females of not only the corresponding species, but also that of Mamestra brassicae. These results indicate that pheromone biosynthesis of the three Plusiinae species is also controlled by a PBAN-like substance. However, the Plusiinae females exceptionally contained remarkable amounts of the pheromone even 1 day after decapitation. Since it has been reported that pheromones completely disappear at least 1 day after decapitation in females of many other lepdidoptran species including B. mori and M. brassicae, a different mechanism is likely regarding the regulation of the studied Plusiinae pheromone biosynthesis. Furthermore, an incorporation experiment with a labeled pheromone precursor, D9-(Z)-7-dodecenoic acid, showed that moderate biosynthesis still proceeded in the pheromone glands of M. confusa females 1 day after decapitation, providing an evidence why complete disappearance of the pheromone was not observed in the females which otherwise lacked a source of the pheromonotropic neuropeptide.
Topics: Animals; Deuterium; Female; Mass Spectrometry; Moths; Neuropeptides; Sex Attractants; Species Specificity
PubMed: 11129587
DOI: 10.1271/bbb.64.2145 -
Journal of Lipid Research Nov 1968Positional isomers of decenoic, dodecenoic, and tetradecenoic acids of human adipose tissue have been separated by gas-liquid chromatography and their amounts determined...
Positional isomers of decenoic, dodecenoic, and tetradecenoic acids of human adipose tissue have been separated by gas-liquid chromatography and their amounts determined by oxidative cleavage. The following acids have been shown to be present: 7-decenoic, 8-decenoic, 9-decenoic, 7-, 8-, and 9-dodecenoic, and 7-, 8-, 9-, and 11-tetradecenoic acids. Among all isomers of the even-numbered acids from C(10) to C(18) the cis-9-isomer predominates. With increasing chain length, however, the content of 9-isomer decreases and the number of isomers increases. No 3- or 5-enoic acids could be detected. The origin and biosynthesis of all isomers are discussed.
Topics: Adipose Tissue; Aged; Alkenes; Chemical Phenomena; Chemistry; Chromatography, Gas; Fatty Acids; Female; Humans; Periodic Acid
PubMed: 4300983
DOI: No ID Found -
The Biochemical Journal May 1995The metabolism of cis-5 unsaturated fatty acids was studied in intact rat liver mitochondria to assess the operation of a reduction pathway. By using direct...
The metabolism of cis-5 unsaturated fatty acids was studied in intact rat liver mitochondria to assess the operation of a reduction pathway. By using direct quantification of metabolites with a capillary-column gas chromatography, 3-hydroxydodecanoate was identified among other metabolites when cis-5-dodecenoate was metabolized in intact rat liver mitochondria. The formation of 3-hydroxydodecanoate supports the existence of a reduction pathway in the metabolism of cis-5-unsaturated fatty acids. This metabolite cannot be produced from the conventional isomerase-mediated pathway. However, the data also indicated the possible operation of the conventional isomerase-mediated pathway in intact rat liver mitochondria. The reduction pathway appears to account for at least 61% of the pathway for cis-5-dodecenoate. This reduction pathway was likely to proceed from the dehydrogenation to trans-2,cis-5-dodecadienoyl-CoA, which was isomerized to delta 3, delta 5-dodecadienoyl-CoA, then to trans-2,trans-4-dodecadienoate. The reduction was mediated by 2,4-dienoyl-CoA reductase by the conversion of trans-2,trans-4-dodecadienoyl-CoA into trans-3-dodecenoyl-CoA. However, direct reduction of the cis-5 double bond was also shown to be operating, although to a lesser extent.
Topics: Animals; Fatty Acids, Monounsaturated; Fatty Acids, Unsaturated; In Vitro Techniques; Mitochondria, Liver; NADP; Oxidation-Reduction; Oxygen Consumption; Rats; Rats, Sprague-Dawley
PubMed: 7755586
DOI: 10.1042/bj3080039 -
FEBS Letters May 1987The metabolism of the double bonds at the delta 3 position in fatty acids was studied in rat liver. Infusion of delta 3-trans-dodecenoic acid into isolated perfused...
The metabolism of the double bonds at the delta 3 position in fatty acids was studied in rat liver. Infusion of delta 3-trans-dodecenoic acid into isolated perfused liver and subcellular fractionation studies showed the presence of both peroxisomal and mitochondrial delta 3,delta 2-enoyl-CoA isomerase activity (EC 5.3.3.8). These findings together with the previous demonstration of peroxisomal 2,4-dienoyl-CoA reductase (EC 1.3.1.34) [(1981) J. Biol. Chem. 256, 8259-8262] and D-3-OH-acyl-CoA epimerase (EC 5.1.2.3) [(1985) FEBS Lett. 185, 129-134] activities show that peroxisomes possess all the auxiliary enzymes required for the beta-oxidation of unsaturated fatty acids.
Topics: Animals; Carbon-Carbon Double Bond Isomerases; Centrifugation; Dodecenoyl-CoA Isomerase; Fatty Acids, Unsaturated; Isomerases; Liver; Male; Microbodies; Mitochondria, Liver; Oxidation-Reduction; Rats; Rats, Inbred Strains; Subcellular Fractions
PubMed: 3582650
DOI: 10.1016/0014-5793(87)80151-5