-
Microbiology Resource Announcements Feb 2024We report here the genome sequence of moderately halophilic ASL-17, isolated from hypersaline sediment from the Yellow Sea, Korea. The bacterium was Gram variable, oval...
We report here the genome sequence of moderately halophilic ASL-17, isolated from hypersaline sediment from the Yellow Sea, Korea. The bacterium was Gram variable, oval or coccoid, and mesophilic. The genome of ASL-17 has 3.8 Mbp, with 3,910 coding sequences, 76 RNAs, and 41.3% G + C content.
PubMed: 38206017
DOI: 10.1128/mra.00692-23 -
Environmental Microbiome Mar 2023The adaptability of halophytes to increased soil salinity is related to complex rhizosphere interactions. In this study, an integrative approach, combining...
The adaptability of halophytes to increased soil salinity is related to complex rhizosphere interactions. In this study, an integrative approach, combining culture-independent and culture-dependent techniques was used to analyze the bacterial communities in the endorizosphere of indigenous succulent halophytes Salicornia europaea, Suaeda maritima, and Camphorosma annua from the natural salt marshes of Slano Kopovo (Serbia). The 16 S rDNA analyses gave, for the first time, an insight into the composition of the endophytic bacterial communities of S. maritima and C. annua. We have found that the composition of endophyte microbiomes in the same habitat is to some extent influenced by plant species. A cultivable portion of the halophyte microbiota was tested at different NaCl concentrations for the set of plant growth promoting (PGP) traits. Through the mining of indigenous halotolerant endophytes, we obtained a collection representing a core endophyte microbiome conferring desirable PGP traits. The majority (65%) of the selected strains belonged to the common halotolerant/halophilic genera Halomonas, Kushneria, and Halobacillus, with representatives exhibiting multiple PGP traits, and retaining beneficial traits in conditions of the increased salinity. The results suggest that the root endosphere of halophytes is a valuable source of PGP bacteria supporting plant growth and fitness in salt-affected soils.
PubMed: 36934265
DOI: 10.1186/s40793-023-00477-x -
New Microbes and New Infections Sep 2020Strain Marseille-Q1234 is a new species from the genus that was isolated in 2019 from a stool sample in a healthy Malian child <5 years old. Cells are Gram-positive and...
Strain Marseille-Q1234 is a new species from the genus that was isolated in 2019 from a stool sample in a healthy Malian child <5 years old. Cells are Gram-positive and strictly halophilic bacilli. Strain Marseille-Q1234 exhibits 98.46% 16S rRNA gene sequence similarity to strain JSM 071068 (NR_116505.1), the phylogenetically closely related species with standing in nomenclature. Based on the phenotypic and phylogenetic evidence, OrthoANI values and results of the biochemical tests, the new species is named sp. nov., for which strain Marseille-Q1234 (= CSURQ1234) is proposed as the type strain.
PubMed: 32793353
DOI: 10.1016/j.nmni.2020.100708 -
FEMS Microbiology Ecology Apr 2004We investigated the precipitation of carbonates by Halobacillus trueperi in both solid and liquid media at different salt concentrations and different magnesium/calcium...
We investigated the precipitation of carbonates by Halobacillus trueperi in both solid and liquid media at different salt concentrations and different magnesium/calcium ratios. H. trueperi precipitated at all assayed salt concentrations. When salt concentration increased, the quantity and the size of bioliths decreased and the time required increased. The precipitated minerals (determined by X-ray diffraction) were calcite, magnesium calcite and monohydrocalcite in variable proportions depending on the salinity and the physical state of the medium; the magnesium content of the magnesium calcites also varied with regard to the culture type. According to the saturation indices other minerals could also precipitate. Scanning electron microscopy showed that dominant morphologies of the bioliths were spherulitic with fibrous radiated interiors. We show that H. trueperi plays an active role in the precipitation of carbonates and we hypothesize about this process of biomineralization.
Topics: Bacillaceae; Calcium; Calcium Carbonate; Carbonates; Culture Media; Magnesium; Microscopy, Electron, Scanning; Salts
PubMed: 19712429
DOI: 10.1016/j.femsec.2003.12.008 -
Applied and Environmental Microbiology Oct 2001Several moderately halophilic gram-positive, spore-forming bacteria have been isolated by conventional enrichment cultures from damaged medieval wall paintings and...
Several moderately halophilic gram-positive, spore-forming bacteria have been isolated by conventional enrichment cultures from damaged medieval wall paintings and building materials. Enrichment and isolation were monitored by denaturing gradient gel electrophoresis and fluorescent in situ hybridization. 16S ribosomal DNA analysis showed that the bacteria are most closely related to Halobacillus litoralis. DNA-DNA reassociation experiments identified the isolates as a population of hitherto unknown Halobacillus species.
Topics: Austria; Construction Materials; Culture Media; Electrophoresis; Gram-Positive Bacteria; In Situ Hybridization, Fluorescence; Molecular Sequence Data; Paintings; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA
PubMed: 11571198
DOI: 10.1128/AEM.67.10.4891-4895.2001 -
Frontiers in Microbiology 2018Arginine-aspartate-aspartate (RDD) family, representing a category of transmembrane proteins containing one highly conserved arginine and two highly conserved...
Arginine-aspartate-aspartate (RDD) family, representing a category of transmembrane proteins containing one highly conserved arginine and two highly conserved aspartates, has been functionally uncharacterized as yet. Here we present the characterization of a member of this family designated RDD from the moderate halophile NEAU-ST10-40 and report for the first time that RDD should function as a novel Na(Li, K)/H antiporter. It's more interesting whether the highly conserved arginine/aspartate residues among the whole family or between RDD and its selected homologs are related to the protein function. Therefore, we analyzed their roles in the cation-transporting activity through site-directed mutagenesis and found that D154, R124, R129, and D158 are indispensable for Na(Li, K)/H antiport activity whereas neither R35 nor D42 is involved in Na(Li, K)/H antiport activity. As a dual representative of Na(Li, K)/H antiporters and RDD family proteins, the characterization of RDD and the analysis of its important residues will positively contribute to the knowledge of the cation-transporting mechanisms of this novel antiporter and the roles of highly conserved arginine/aspartate residues in the functions of RDD family proteins.
PubMed: 29922240
DOI: 10.3389/fmicb.2018.00807 -
Heliyon Nov 2018Cultivable halophilic microorganisms were isolated and identified from saline and alkaline-sodic soils: Cuatro Cienegas, Sayula and San Marcos lakes. Physicochemical...
Cultivable halophilic microorganisms were isolated and identified from saline and alkaline-sodic soils: Cuatro Cienegas, Sayula and San Marcos lakes. Physicochemical characteristics of soils were determined to understand the relationship between those and the microorganisms isolated. The Cuatro Cienegas soils had a neutral pH, EC of 2.3-8 dS cm, classified as moderately saline. Whereas, the soils from Sayula and San Marcos lakes, had an alkaline pH, EC 15 to 65 dS m, typical of saline-sodic. We identified 23 cultivable halophilic bacteria using 16s rDNA, being sp., sp., and sp. the predominant genus by culture dependent approach. We found a correlation between the soils anion and cation content with the occurrence of different genus of halophilic bacteria in each studied site. sp. was predominant in Sayula and San Marcos lakes and was related to the high Na content; while sp. and sp. were predominant in Cuatro Cienegas, their occurrence was related to a high content of Ca, Mg, and SO .
PubMed: 30519656
DOI: 10.1016/j.heliyon.2018.e00954 -
Microbes and Environments Jun 2017The aim of the present study was to obtain insights into the relationship between the chemical (salt content and pH) and physico-mechanical (humidity and compressive...
The aim of the present study was to obtain insights into the relationship between the chemical (salt content and pH) and physico-mechanical (humidity and compressive strength) properties of mineral-based materials from historic buildings with salt efflorescence and the growth and biodiversity of halophilic microorganisms. Samples were mainly characterized by pH 6.5-8.5 and a moisture content of between 0.12 and 3.3%. Significant variations were also found in the salt content (sulfates, chlorides, and nitrates) of the materials. An SEM/EDS analysis of material surfaces revealed the presence of halite, calcite, gypsum, sodium sulfate, and potassium-sodium sulfate. Culture-dependent and culture-independent (clone library construction) approaches were both applied to detect halophilic microorganisms. Results derived from culturable methods and the materials analysis revealed a correlation between the total halophile count and pH value as well as sulfate content. A correlation was not observed between the concentration of chlorides or nitrates and the number of halophilic microorganisms. The materials studied were inhabited by the culturable halophilic bacteria Halobacillus sp., Virgibacillus sp., and Marinococcus sp. as well as the yeast Sterigmatomyces sp., which was isolated for the first time from mineral materials. Culture-independent techniques revealed the following bacterial species: Salinibacterium, Salinisphaera, Rubrobacter, Rubricoccus, Halomonas, Halorhodospira, Solirubrobacter, Salinicoccus, and Salinibacter. Biodiversity was the highest in materials with high or moderate salinity.
Topics: Bacteria; Biodiversity; Compressive Strength; Construction Materials; Humidity; Hydrogen-Ion Concentration; Salts
PubMed: 28592721
DOI: 10.1264/jsme2.ME16159 -
Brazilian Journal of Microbiology :... Jan 2019Microbes from hypersaline environments are useful in biotechnology as sources of novel enzymes and proteins. The current study aimed to characterize halophilic bacteria...
Microbes from hypersaline environments are useful in biotechnology as sources of novel enzymes and proteins. The current study aimed to characterize halophilic bacteria from the rhizosphere of halophytes (Salsola stocksii and Atriplex amnicola), non-rhizospheric, and brine lake-bank soils collected from Khewra Salt Mine and screening of these bacterial strains for industrially important enzymes. A total of 45 bacterial isolates from the rhizosphere of Salsola, 38 isolates from Atriplex, 24 isolates from non-rhizospheric, and 25 isolates from lake-bank soils were identified by using 16S rRNA gene analysis. Phylogenetic analysis showed that bacterial strains belonging to Bacillus, Halobacillus, and Kocuria were dominant in the rhizosphere of halophytes (Salsola and Atriplex), and Halobacillus and Halomonas were dominating genera from non-rhizospheric and lake-bank soils. Mostly identified strains were moderately halophilic bacteria with optimum growth at 1.5-3.0 M salt concentrations. Most of the bacterial exhibited lipase, protease, cellulase, amylase, gelatinase, and catalase activities. Halophilic and halotolerant Bacilli (AT2RP4, HL1RS13, NRS4HaP9, and LK3HaP7) identified in this study showed optimum lipase, protease, cellulase, and amylase activities at 1.0-1.5 M NaCl concentration, pH 7-8, and temperature 37 °C. These results indicated that halophilic and halotolerant bacteria can be used for bioconversion of organic compounds to useful products under extreme conditions.
Topics: Atriplex; Bacteria; Bacterial Proteins; Biodiversity; Cellulases; Hydrolases; Lakes; Lipase; Peptide Hydrolases; Phylogeny; Rhizosphere; Sodium Chloride; Soil Microbiology
PubMed: 30623303
DOI: 10.1007/s42770-019-00044-y -
Life (Basel, Switzerland) Feb 2013The capability of osmoadaptation is a prerequisite of organisms that live in an environment with changing salinities. Halobacillus halophilus is a moderately halophilic... (Review)
Review
The capability of osmoadaptation is a prerequisite of organisms that live in an environment with changing salinities. Halobacillus halophilus is a moderately halophilic bacterium that grows between 0.4 and 3 M NaCl by accumulating both chloride and compatible solutes as osmolytes. Chloride is absolutely essential for growth and, moreover, was shown to modulate gene expression and activity of enzymes involved in osmoadaptation. The synthesis of different compatible solutes is strictly salinity- and growth phase-dependent. This unique hybrid strategy of H. halophilus will be reviewed here taking into account the recently published genome sequence. Based on identified genes we will speculate about possible scenarios of the synthesis of compatible solutes and the uptake of potassium ion which would complete our knowledge of the fine-tuned osmoregulation and intracellular osmolyte balance in H. halophilus.
PubMed: 25371341
DOI: 10.3390/life3010234