-
Progress in Neuro-psychopharmacology &... Jan 2023Methylglyoxal (MGO) is a reactive dicarbonyl compound formed as a byproduct of glycolysis. MGO is a major cell-permeant precursor of advanced glycation end products... (Review)
Review
Methylglyoxal (MGO) is a reactive dicarbonyl compound formed as a byproduct of glycolysis. MGO is a major cell-permeant precursor of advanced glycation end products (AGEs), since it readily reacts with basic phospholipids and nucleotides, as well as amino acid residues of proteins, such as arginine, cysteine, and lysine. The AGEs production induced by MGO are widely associated with several pathologies, including neurodegenerative diseases. However, the impact of MGO metabolism and AGEs formation in the central nervous system (particularly in neurons, astrocytes and oligodendrocytes) on behavior and psychiatric diseases is not fully understood. Here, we briefly present background information on the biological activity of MGO in the central nervous system. It was gathered the available information on the role of MGO metabolism at the physiological processes, as well as at the neurobiology of psychiatry diseases, especially pain-related experiences, anxiety, depression, and cognition impairment-associated diseases. To clarify the role of MGO on behavior and associated diseases, we reviewed primarily the main findings at preclinical studies focusing on genetic and pharmacological approaches. Since monoamine neurotransmitter systems are implicated as pivotal targets on the pathophysiology and treatment of psychiatry and cognitive-related diseases, we also reviewed how MGO affects these neurotransmission systems and the implications of this phenomenon for nociception and pain; learning and cognition; and mood. In summary, this review highlights the pivotal role of glyoxalase 1 (Glo1) and MGO levels in modulating behavioral phenotypes, as well as related cellular and molecular signaling. Conclusively, this review signals dopamine as a new neurochemical MGO target, as well as highlights how MGO metabolism can modulate the pathophysiology and treatment of pain, psychiatric and cognitive-related diseases.
Topics: Humans; Pyruvaldehyde; Glycation End Products, Advanced; Cysteine; Dopamine; Lysine; Magnesium Oxide; Mental Disorders; Pain; Arginine; Nucleotides
PubMed: 36103947
DOI: 10.1016/j.pnpbp.2022.110635 -
Molecules (Basel, Switzerland) Nov 2022Advances in molecular biology technology have piqued tremendous interest in glycometabolism and bioenergetics in homeostasis and neural development linked to ageing and... (Review)
Review
Advances in molecular biology technology have piqued tremendous interest in glycometabolism and bioenergetics in homeostasis and neural development linked to ageing and age-related diseases. Methylglyoxal (MGO) is a by-product of glycolysis, and it can covalently modify proteins, nucleic acids, and lipids, leading to cell growth inhibition and, eventually, cell death. MGO can alter intracellular calcium homeostasis, which is a major cell-permeant precursor to advanced glycation end-products (AGEs). As side-products or signalling molecules, MGO is involved in several pathologies, including neurodevelopmental disorders, ageing, and neurodegenerative diseases. In this review, we demonstrate that MGO (the metabolic side-product of glycolysis), the GLO system, and their analogous relationship with behavioural phenotypes, epigenetics, ageing, pain, and CNS degeneration. Furthermore, we summarise several therapeutic approaches that target MGO and the glyoxalase (GLO) system in neurodegenerative diseases.
Topics: Pyruvaldehyde; Lactoylglutathione Lyase; Magnesium Oxide; Glycolysis; Brain
PubMed: 36432007
DOI: 10.3390/molecules27227905 -
Scientific Reports Feb 2020Asian citrus psyllid (Diaphorina citri) vectors the bacterium Candidatus Liberibacter asiaticus, the causal pathogen of citrus greening disease that is devastating...
Asian citrus psyllid (Diaphorina citri) vectors the bacterium Candidatus Liberibacter asiaticus, the causal pathogen of citrus greening disease that is devastating citrus industries worldwide. Suppressing psyllid populations is crucial to prevent disease spread. An attract-and-kill trap based on psyllid behavior would fill a niche for monitoring and control. To optimize visual attraction of psyllids, the ultraviolet (UV) reflective properties of magnesium oxide (MgO) and/or barium sulfate (BaSO) were assessed for potential application to a trap surface. Under low UV, high UV and natural sunlight conditions, the reflectance, attraction, and probing behaviors of psyllids were evaluated on surfaces containing magnesium oxide or barium sulfate. Magnesium oxide added to yellow sticky traps enhanced visual response of D. citri. Probing assays demonstrated that magnesium oxide alone or as a mixture with a phagostimulant blend, increased the UV reflectance of substrates, as well as, attraction and probing by psyllids. Results demonstrated that psyllids respond to both short (UV) and long (yellow) wavelengths during orientation, and that these inert compounds can increase UV reflectance and improve attractiveness of an attract-and-kill device.
Topics: Animals; Behavior, Animal; Biological Assay; Citrus; Crop Protection; Cues; Hemiptera; Insect Control; Insect Vectors; Magnesium Oxide; Plant Diseases; Rhizobiaceae; Ultraviolet Rays; Vision, Ocular
PubMed: 32024952
DOI: 10.1038/s41598-020-58593-4 -
Ecotoxicology and Environmental Safety Sep 2021Lead (Pb) and cadmium (Cd) are considered as a typical heavy metals in aqueous solution, which may pose adverse health effects on human beings. For the removal of these...
Lead (Pb) and cadmium (Cd) are considered as a typical heavy metals in aqueous solution, which may pose adverse health effects on human beings. For the removal of these two pollutants, magnesium oxide (MgO) was successfully immobilized onto eucalyptus biochar (BC) matrix via simple and cost-effective pyrolysis process of MgCl-pretreated eucalyptus biomass under high temperature (500 °C). Synthesized MgO nanoparticles-biochar composites (MBC) exhibited superior removal performance for target pollutants, and achieve 99.9% removal efficiency for Pb(II) and Cd(II) at optimum conditions (0.02 g, pH in range of 4-7, and reaction time 120, 240 min). Furthermore, the maximum theoretical adsorbing amount of MBC was 829.11 mg/g for Pb(II) and 515.17 mg/g for Cd(II). Pseudo-second-order model and Langmuir models were well-determined for isotherm and adsorption kinetics. FTIR, XRD, and XPS analysis revealed that precipitation and ion exchange was of great importance for the removal of contaminants. Besides, cation-π interaction and complexation from the carbon-containing functional groups should not be neglected. Considering the advantage of low-cost, facile preparation, and brilliant adsorption capacity, it is anticipated that MBC has a promising prospect for the broad application in Pb(II)/Cd(II)-containing wastewater treatment.
Topics: Adsorption; Cadmium; Charcoal; Eucalyptus; Lead; Magnesium Oxide; Solutions; Water Pollutants, Chemical; Water Purification
PubMed: 34166940
DOI: 10.1016/j.ecoenv.2021.112426 -
International Journal of Molecular... Apr 2022The accurate segregation of sister chromatids is complex, and errors that arise throughout this process can drive chromosomal instability and tumorigenesis. We recently...
The accurate segregation of sister chromatids is complex, and errors that arise throughout this process can drive chromosomal instability and tumorigenesis. We recently showed that methylglyoxal (MGO), a glycolytic by-product, can cause chromosome missegregation events in lymphocytes. However, the underlying mechanisms of this were not explored. Therefore, in this study, we utilised shotgun proteomics to identify MGO-modified proteins, and label-free quantitation to measure changes in protein abundance following exposure to MGO. We identified numerous mitotic proteins that were modified by MGO, including those involved in the separation and cohesion of sister chromatids. Furthermore, the protein abundance of Securin, an inhibitor of sister chromatid separation, was increased following treatment with MGO. Cytological examination of chromosome spreads showed MGO prevented sister chromatid separation, which was associated with the formation of complex nuclear anomalies. Therefore, results from this study suggest MGO may drive chromosomal instability by preventing sister chromatid separation.
Topics: Cell Cycle Proteins; Chromatids; Chromosomal Instability; Chromosome Segregation; Humans; Lymphocytes; Magnesium Oxide; Pyruvaldehyde
PubMed: 35456956
DOI: 10.3390/ijms23084139 -
Materials (Basel, Switzerland) Jan 2023A series of calcium oxide-magnesium oxide (CaO-MgO) catalysts were prepared under the effects of different precipitating agents and using varied Mg/Ca ratios. The...
A series of calcium oxide-magnesium oxide (CaO-MgO) catalysts were prepared under the effects of different precipitating agents and using varied Mg/Ca ratios. The physiochemical characteristics of the prepared catalysts were analyzed using XRD, FE-SEM, BET, FTIR, and TG/DTA techniques. Quantification of basic active sites present on the surface of the CaO-MgO catalysts was carried out using the Hammett indicator method. The as-prepared mixed oxide samples were tested for propylene carbonate (PC) synthesis through the alcoholysis of urea with propylene glycol (PG). The effects of the catalyst composition, catalyst dose, reaction temperature, and contact time on the PC yield and selectivity were investigated. The maximum PC yield of 96%, with high PC selectivity of 99% and a urea conversion rate of 96%, was attained at 160 °C using CaO-MgO catalysts prepared using a Mg/Ca ratio of 1 and NaCO as a precipitating agent. The best-performing catalysts also exhibited good reusability without any significant loss in PC selectivity. It is expected that the present study will provide useful information on the suitability of different precipitating agents with respect to the catalytic properties of the oxides of Ca and Mg and their application in the synthesis of organic carbonates.
PubMed: 36676471
DOI: 10.3390/ma16020735 -
Cells Apr 2023Glioblastoma (GBM) is a highly aggressive and invasive brain tumor with a poor prognosis despite extensive treatment. The switch to aerobic glycolysis, known as the...
Glioblastoma (GBM) is a highly aggressive and invasive brain tumor with a poor prognosis despite extensive treatment. The switch to aerobic glycolysis, known as the Warburg effect, in cancer cells leads to an increased production of methylglyoxal (MGO), a potent glycation agent with pro-tumorigenic characteristics. MGO non-enzymatically reacts with proteins, DNA, and lipids, leading to alterations in the signaling pathways, genomic instability, and cellular dysfunction. In this study, we investigated the impact of MGO on the LN229 and U251 (WHO grade IV, GBM) cell lines and the U343 (WHO grade III) glioma cell line, along with primary human astrocytes (hA). The results showed that increasing concentrations of MGO led to glycation, the accumulation of advanced glycation end-products, and decreasing cell viability in all cell lines. The invasiveness of the GBM cell lines increased under the influence of physiological MGO concentrations (0.3 mmol/L), resulting in a more aggressive phenotype, whereas glycation decreased the invasion potential of hA. In addition, glycation had differential effects on the ECM components that are involved in the invasion progress, upregulating TGFβ, brevican, and tenascin C in the GBM cell lines LN229 and U251. These findings highlight the importance of further studies on the prevention of glycation through MGO scavengers or glyoxalase 1 activators as a potential therapeutic strategy against glioma and GBM.
Topics: Humans; Glioblastoma; Maillard Reaction; Magnesium Oxide; Glioma; Brain Neoplasms; Neoplastic Processes
PubMed: 37174618
DOI: 10.3390/cells12091219 -
Scientific Reports Jul 2022In this study, we investigated the process of preconcentrate and determine trace amounts of Auramine O (AO) and methylene blue (MB) dyes in environmental water samples....
In this study, we investigated the process of preconcentrate and determine trace amounts of Auramine O (AO) and methylene blue (MB) dyes in environmental water samples. For this purpose, the ultrasound-assisted dispersive-magnetic nanocomposites-solid-phase microextraction (UA-DMNSPME) method was performed to extract AO and MB from aqueous samples by applying magnesium oxide nanoparticles (MgO-NPs). The proposed technique is low-cost, facile, fast, and compatible with many existing instrumental methods. Parameters affecting the extraction of AO and MB were optimized using response surface methodology (RSM). Short extraction time, low experimental tests, low consumption of organic solvent, low limits of detection (LOD), and high preconcentration factor (PF) was the advantages of method. The PF was 44.5, and LOD for AO and MB was 0.33 ng mL and 1.66 ng mL, respectively. The linear range of this method for AO and MB were 1-1000 ng mL and 5-2000 ng mL, respectively. In addition, the relative standard deviation (RSD; n = 5) of the mentioned analytes was between 2.9% and 3.1%. The adsorption-desorption studies showed that the efficiency of adsorbent extraction had not declined significantly up to 6 recycling runs, and the adsorbent could be used several times. The interference studies revealed that the presence of different ions did not interfere substantially with the extraction and determination of AO and MB. Therefore, UA-DMNSPME-UV/Vis method can be proposed as an efficient method for preconcentration and extraction of AO and MB from water and wastewater samples.
Topics: Benzophenoneidum; Magnesium Oxide; Methylene Blue; Nanoparticles; Solid Phase Extraction; Solid Phase Microextraction; Water
PubMed: 35896658
DOI: 10.1038/s41598-022-16948-z -
Journal of Dairy Science May 1982Two reagent and two feed grade magnesium oxides and reagent grade magnesium carbonate, sodium bicarbonate, and calcium carbonate were evaluated to ascertain their... (Comparative Study)
Comparative Study
Two reagent and two feed grade magnesium oxides and reagent grade magnesium carbonate, sodium bicarbonate, and calcium carbonate were evaluated to ascertain their ability to neutralize acid in the rumen. Rumen fluid pH was increased in vitro, compared to the control, by antacid compounds, and their increased ranked: calcium carbonate less than feed grade magnesium oxide A less than light magnesium oxide and feed grade magnesium oxide B less than heavy magnesium oxide less than magnesium carbonate less than sodium bicarbonate. Titrations at constant pH's ranging from 3.0 to 7.5 indicated that these magnesium compounds were reactive at pH's normally in the rumen although reactivity decreased with increasing pH. Intraruminal doses of feed grade magnesium oxide A and calcium carbonate did not change rumen fluid pH for other compounds ranked: feed grade magnesium oxide B less than magnesium carbonate less than heavy magnesium oxide. Feeding of heavy magnesium oxide or magnesium carbonate increased rumen fluid pH as compared to the control diet. Feeding feed grade magnesium oxide B in three quantities to cattle resulted in proportional increased in fecal pH and fluidity of feces. Two feed grade magnesium oxides differed in their ability to neutralize acid in the rumen.
Topics: Animals; Bicarbonates; Calcium Carbonate; Cattle; Feces; Female; Hydrogen-Ion Concentration; Magnesium; Magnesium Oxide; Rumen; Sodium Bicarbonate
PubMed: 6286737
DOI: 10.3168/jds.S0022-0302(82)82260-1 -
Scientific Reports Nov 2015Nanomaterial-based photoluminescence (PL) diagnostic devices offer fast and highly sensitive detection of pesticides, DNA, and toxic agents. Here we report a label-free...
Nanomaterial-based photoluminescence (PL) diagnostic devices offer fast and highly sensitive detection of pesticides, DNA, and toxic agents. Here we report a label-free PL genosensor for sensitive detection of Vibrio cholerae that is based on a DNA hybridization strategy utilizing nanostructured magnesium oxide (nMgO; size >30 nm) particles. The morphology and size of the synthesized nMgO were determined by transmission electron microscopic (TEM) studies. The probe DNA (pDNA) was conjugated with nMgO and characterized by X-ray photoelectron and Fourier transform infrared spectroscopic techniques. The target complementary genomic DNA (cDNA) isolated from clinical samples of V. cholerae was subjected to DNA hybridization studies using the pDNA-nMgO complex and detection of the cDNA was accomplished by measuring changes in PL intensity. The PL peak intensity measured at 700 nm (red emission) increases with the increase in cDNA concentration. A linear range of response in the developed PL genosensor was observed from 100 to 500 ng/μL with a sensitivity of 1.306 emi/ng, detection limit of 3.133 ng/μL and a regression coefficient (R(2)) of 0.987. These results show that this ultrasensitive PL genosensor has the potential for applications in the clinical diagnosis of cholera.
Topics: Biosensing Techniques; Cholera; DNA Probes; DNA, Bacterial; DNA, Complementary; Humans; Limit of Detection; Luminescent Measurements; Magnesium Oxide; Nanostructures; Nucleic Acid Hybridization; Photochemical Processes; Spectroscopy, Fourier Transform Infrared; Vibrio cholerae
PubMed: 26611737
DOI: 10.1038/srep17384