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The Korean Journal of Parasitology Dec 1993This study investigate the epidemiological feature of Metagonimus infection in Kangwon-do (province). The average infection rate of the surveyed inhabitants was 7.8% (83...
This study investigate the epidemiological feature of Metagonimus infection in Kangwon-do (province). The average infection rate of the surveyed inhabitants was 7.8% (83 positives out of 1, 067 examinees) by stool examination; male, 11.4% and female, 3.2%, respectively. The egg positive rate in residents in the Som river area was 7.3%, that of the Chuchon river area 6.3%, the Pyongchang river area 12.8%, the Tong river area 3.8%, the Hongchon river area 9.8%, and the Ohsip stream area of Samchok 8.0%, respectively. The average metacercarial infection rate of genus Metagonimus in the fish was 81.0% (256 positives out of 318 fish). The infected fishes were Zacco platypus, Zacco temmincki, Opsariichthys bidens, Squalidis sp., Carassius carassius, etc. in western Kangwon-do Meanwhile, in the Ohsip stream area of Samchok-gun, eastern costal Kangwon-do, the infected fish were Plecoglossus altivelis and Tribolodon hakonensis. The rats and dogs are infected with the metcercariae obtained from Zacco platypus and Opsariichthys bidens, adult worms collected were Miyata type of Metagonimus with some M. takahashii. When infected with metacercariae from Plecoglossus altivelis, Metagonimus yokogawai was only found. M. yokogawai and Metagonimus Miyata type were found together in Tribolodon hakonensis in Ohsip stream area of Samchok, in the eastern Kangwon-do. The intestinal flukes of genus Metagonimus in western Kangwon-do were Miyata type of Metagonimus and M. takahashii, transmitting mainly by Zacco platypus and Opsariichthys bidens as a source of infection. In the eastern part of Kangwon province (Ohsip stream area of Samchok), M. yokogawai was mainly distributed by P. altivelis and T. hakonesis, but some T. hakonensis harbored the metacercariae of Miyata type of Metagonimus with those of M. yokogawai.
Topics: Animals; Child; Dogs; Female; Fishes; Fresh Water; Heterophyidae; Host-Parasite Interactions; Humans; Korea; Male; Prevalence; Rats; Trematode Infections
PubMed: 8297890
DOI: 10.3347/kjp.1993.31.4.331 -
The Korean Journal of Parasitology Mar 1996Taxonomic problems of Metagonimus spp. in Korea were investigated. Metacercariae of various freshwater fish species -- Plecoglossus altivelis, Carassius auratus, Zacco...
Taxonomic problems of Metagonimus spp. in Korea were investigated. Metacercariae of various freshwater fish species -- Plecoglossus altivelis, Carassius auratus, Zacco platypus, Zacco temmincki, Opsariichthys bidens -- were collected from different localities in Korea and experimentally fed to golden hamsters. Observation of recovered adult worms showed that Plecoglossus altivelis was infected with metacercariae of both M. yokogawai and M. takahashii. C. auratus was infected with metacercariae of M. takahashii and Z. platypus, Z. temmincki, O. bidens were infected only with metacercariae of Metagonimus Miyata type. From the inferences about the morphological characteristics, host specificities and occurrence patterns in infected animals, Metagonimus Miyata type is considered to be an independent group.
Topics: Animals; Cricetinae; Fishes; Fresh Water; Heterophyidae; Host-Parasite Interactions; Korea; Mesocricetus; Trematode Infections
PubMed: 8820736
DOI: 10.3347/kjp.1996.34.1.7 -
The Journal of Veterinary Medical... Jun 2021The definitive hosts of Metagonimus hakubaensis are reported to be hamsters, rats, mice, dogs, cats, chickens, and quails in experimental infection and Japanese water...
The definitive hosts of Metagonimus hakubaensis are reported to be hamsters, rats, mice, dogs, cats, chickens, and quails in experimental infection and Japanese water shrews in natural infection. Here we report that raccoon dogs are new natural definitive hosts of M. hakubaensis, based on morphological and molecular analyses of Metagonimus flukes collected from the host species from Aomori Prefecture, Japan. Moreover, M. hakubaensis recovered from raccoon dogs showed higher fecundity than those recovered from Japanese water shrews. Therefore, raccoon dogs were considered as a more suitable natural definitive host of M. hakubaensis than Japanese water shrews.
Topics: Animals; Cats; Chickens; Cricetinae; Heterophyidae; Japan; Mice; Raccoon Dogs; Rats; Trematoda
PubMed: 33840724
DOI: 10.1292/jvms.20-0700 -
Molecular and Biochemical Parasitology Sep 2020Ascocotyle longa is parasitic trematode with wide distribution throughout America, Europe, Africa, and Middle East. Despite the fact that this fish-borne pathogen has...
Ascocotyle longa is parasitic trematode with wide distribution throughout America, Europe, Africa, and Middle East. Despite the fact that this fish-borne pathogen has been considered an agent of human heterophyiasis in Brazil, the molecules involved in the host-parasite interaction remain unknown. The present study reports the proteome profile of A. longa metacercariae collected from the fish Mugil liza from Brazil. This infective stage for humans, mammals and birds was analyzed using nLC-MS/MS approach. We identified a large repertoire of proteins, which are mainly involved in energy metabolism and cell structure. Peptidases and immunogenic proteins were also identified, which might play roles in host-parasite interface. Our data provided unprecedented insights into the biology of A. longa and represent a first step to understand the natural host-parasite interaction. Moreover, as the first proteome characterized in this trematode, it will provide an important resource for future studies.
Topics: Animals; Fish Diseases; Genome, Helminth; Helminth Proteins; Heterophyidae; Host-Parasite Interactions; Metacercariae; Proteome; Proteomics; Trematoda; Trematode Infections; Zoonoses
PubMed: 32745491
DOI: 10.1016/j.molbiopara.2020.111311 -
The Korean Journal of Parasitology Dec 1998A scanning electron microscopic study was performed to observe surface ultrastructures of excysted metacercariae and adults of Metagonimus miyatai. Metacercariae were...
A scanning electron microscopic study was performed to observe surface ultrastructures of excysted metacercariae and adults of Metagonimus miyatai. Metacercariae were collected from the scale of the pale chub (Zacco platypus), and adult flukes were harvested 1-4 weeks after infection to rats. In excysted metacercariae, the oral sucker was devoid of tegumental spines and had type I and type II sensory papillae. Anteriorly to the ventral sucker, spines were dense and digitated into 5-7 points, whereas near the posterior end of the body spines were sparse and digitated into 2-3 points. In one-week adults, 7 type II sensory papillae were arranged around the lip of the oral sucker, and at inner side of the lip one pair of small and two pairs of large type 1 sensory papillae were seen on each side. The distribution of tegumental spines was similar to that of metacercariae, but they were more differentiated with 9-11 pointed tips. In two- to four-week old adults, the surface ultrastructure was nearly the same as in one-week old adults, however, sperms were frequently seen entering into the Laurer's canal. Conclusively, the surface ultrastructure of M. miyatai was generally similar to that of M. yokogawai, however, differentiation of tegumental spines and distribution of sensory papillae around the oral sucker were different between the two species, which may be of taxonomic significance.
Topics: Animals; Fishes; Heterophyidae; Microscopy, Electron, Scanning; Rats; Rats, Sprague-Dawley
PubMed: 9868886
DOI: 10.3347/kjp.1998.36.4.217 -
Parasitology May 2021In this study of Metagonimus suifunensis (M. suifunensis) in the Russian Southern Far East, the variability of the full-length sequences of the cytochrome b (cytb) mtDNA...
In this study of Metagonimus suifunensis (M. suifunensis) in the Russian Southern Far East, the variability of the full-length sequences of the cytochrome b (cytb) mtDNA gene was assessed for the first time. In addition, the cox1 mtDNA gene sequences were also obtained for this species from new localities. In total, 87 and 81 sequences of the cytb and cox1 genes, respectively, were used in the current study. The cytb gene proved more promising and revealed two haplogroups that are associated with the spatial distribution of the species: geographical isolation caused the fixation of differences between northern and southern populations. In addition, the results obtained for the cytb gene opened up new perspectives in the analysis of sequences of the cox1 gene, which was not sufficiently effective as a sole marker. Based on data for both mitochondrial genes, molecular processes influencing the formation of the modern population were analysed for M. suifunensis. The new data confirmed the previously expressed opinion that this species colonized the study territory from north to south and will form the basis for determining possible ways of its further expansion, which is important for predicting the emergence of new foci of metagonimosis.
Topics: Amino Acid Sequence; Animals; Base Sequence; Cytochromes b; Disease Vectors; Genetic Markers; Genetic Variation; Haplotypes; Heterophyidae; Male; Rats; Rivers; Russia; Trematode Infections
PubMed: 33583451
DOI: 10.1017/S0031182021000275 -
Animals : An Open Access Journal From... Dec 2023In recent years, the consumption of fish products has surged in European countries, being an essential part of a healthy diet. Despite representing a small part of EU... (Review)
Review
In recent years, the consumption of fish products has surged in European countries, being an essential part of a healthy diet. Despite representing a small part of EU production, freshwater fisheries hold considerable significance for lake-dwelling populations and tourists seeking traditional dishes. This increased fish consumption has brought to light potential health risks associated with fish-borne zoonotic helminths (FBZHs), now acknowledged as global food-borne parasites. Fish-borne zoonotic helminths belong to various taxonomic groups, including nematodes (Anisakidae), trematodes (Opisthorchiidae and Heterophyidae), and cestodes (Diphyllobothriidae). More than 50 species of FBZH are known to cause human infections, derived from eating raw or undercooked aquatic foods containing viable parasites. Despite increased attention, FBZHs remain relatively neglected compared to other food-borne pathogens due to factors like chronic disease progression and under-diagnosis. This systematic review concentrates on the prevalence of six freshwater FBZHs (, , , , , and ) in Italy and neighbouring countries. The study explores the expansion of these parasites, analysing their biological and epidemiological aspects, and the factors that influence their proliferation, such as the increased cormorant population and the lake eutrophication phenomena. In summary, this research highlights the necessity for further research, the development of spatial databases, and the establishment of a unified European policy to effectively manage these multifaceted health concerns. It strongly advocates adopting a One-Health approach to address the growing incidence of parasitic zoonoses within the context of food safety in EU countries.
PubMed: 38136832
DOI: 10.3390/ani13243793 -
The Korean Journal of Parasitology Jun 1993An epidemiological study of Metagonimus infection was undertaken along the upper reaches of the Namhan River, with special consideration on the species (type) of the... (Comparative Study)
Comparative Study
An epidemiological study of Metagonimus infection was undertaken along the upper reaches of the Namhan River, with special consideration on the species (type) of the worms collected from humans. Eggs of Metagonimus spp. were detected from 15 (9.7%) of 154 people examined in Umsong-gun, and from each infected person (5 cases) 6,015-24,060 worms (mean 13,233) were recovered after treatment with praziquantel (10 mg/kg). Eggs were also detected from 37 (48.1%) of 77 people in Yongwol-gun, from whom (27 cases) 1-4,965 worms (means 1,215) were collected. The worm from Umsong-gun consisted of both Metagonimus Miyata type and Metagonimus takahashii, whereas those from Yongwol-gun consisted of only Metagonimus Miyata type. When the uterine eggs of the two kinds and M. yokogawai (obtained from people in Tamjin River basin) were morphologically compared, it was suggested that the egg size should be a good indicator for discrimination of the species or type. The source of human infection was proved to be fresh water fishes; 49 of 52 Zacco platypus examined, 6 of 8 Hemibarbus longirostris, 13 of 15 Pseudogobio esocinus, 4 of 6 Odontobutis obscura interrupta, and 17 of 18 Carassius carassius were found infected with Metagonimus metacercariae. From the results, it is concluded that the upper reaches of the Namhan River are endemic foci of Metagonimus Miyata type and M. takahashii.
Topics: Animals; Female; Fishes; Fresh Water; Heterophyidae; Humans; Korea; Male; Parasite Egg Count; Trematode Infections
PubMed: 8343462
DOI: 10.3347/kjp.1993.31.2.99 -
The Korean Journal of Parasitology Dec 1995To evaluate the feasibility of irradiation as a control measure for metagonimiasis, the metacercariae of Metagonimus yokogawai were irradiated with gamma ray, either...
To evaluate the feasibility of irradiation as a control measure for metagonimiasis, the metacercariae of Metagonimus yokogawai were irradiated with gamma ray, either after isolation from the sweetfish (Plecoglossus altivelis) or in situ of the fish, and their survival and development in rats were observed at 7 days post-infection. The radiation dose varied from 5 to 100 Gy for the metacercaria-irradiation group and from 5 to 500 Gy for fish-irradiation group. The results showed that the worm recovery rate from the irradiation groups decreased as the radiation dose was increased. Higher doses of radiation were required for the fish-irradiation group to obtain the same results as the metacercaria-irradiation group. The LD50 of the metacercaria-irradiation group was 4.5 Gy, whereas that of the fish-irradiation group 6.2 Gy. A few number of worms which survived until 7 days in rats were severely retarded especially in the growth of their reproductive organs, i.e., complete or partial failure in the development of testes and formation of uterine eggs. The present study revealed that irradiation of sweetfish by 200 Gy is effective to control infectivity as well as development of M. yokogawai metacercaria in rats.
Topics: Animals; Fishes; Gamma Rays; Heterophyidae; Radiation Dosage; Rats; Rats, Sprague-Dawley; Trematode Infections
PubMed: 8591007
DOI: 10.3347/kjp.1995.33.4.297 -
The Korean Journal of Parasitology Mar 2000A small-scale epidemiological survey was undertaken during 1997-1998 on the residents along the Osib-chon (Stream), Samchok-shi (City), Kangwon-do (Province), to...
A small-scale epidemiological survey was undertaken during 1997-1998 on the residents along the Osib-chon (Stream), Samchok-shi (City), Kangwon-do (Province), to evaluate the status of Metagonimus yokogawai infection. A total of 165 fecal samples was collected and examined by cellophane thick smear and formalin-ether sedimentation techniques. The egg positive rate of M. yokogawai was 29.7%, showing a remarkable difference between males (46.6%) and females (16.3%). To obtain the adult flukes of M. yokogawai, 11 egg positive persons were treated with praziquantel and purged with magnesium sulfate. A total of 242,119 adult flukes (average 22,010 per person, 367-119,650 in range) was collected from diarrheic stools, all of which were identified as M. yokogawai. The results show that M. yokogawai is still highly endemic in this area.
Topics: Adolescent; Adult; Aged; Animals; Child; Child, Preschool; Endemic Diseases; Feces; Female; Heterophyidae; Humans; Infant; Intestinal Diseases, Parasitic; Korea; Male; Middle Aged; Parasite Egg Count; Trematode Infections
PubMed: 10743357
DOI: 10.3347/kjp.2000.38.1.33