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Journal of Food Science and Technology Oct 2016Current study was designed to find out how tea harvesting time affects the volatile and non-volatile compounds profiles of green tea. In addition, correlation of...
Current study was designed to find out how tea harvesting time affects the volatile and non-volatile compounds profiles of green tea. In addition, correlation of instrumental volatile and non-volatile compounds analyses to consumer perception were analyzed. Overall, earlier harvested green tea had stronger antioxidant capacity (~61.0%) due to the polyphenolic compounds from catechin (23,164 mg/L), in comparison to later harvested green teas (11,961 mg/L). However, high catechin content in green tea influenced negatively the consumer likings of green tea, due to high bitterness (27.6%) and astringency (13.4%). Volatile compounds drive consumer liking of green tea products were also identified, that included linalool, 2,3-methyl butanal, 2-heptanone, (E,E)-3,5-Octadien-2-one. Finding from current study are useful for green tea industry as it provide the difference in physiochemical properties of green tea harvested at different intervals.
PubMed: 28017988
DOI: 10.1007/s13197-016-2349-y -
Turkish Journal of Chemistry 2023This study focuses on the modification of natural hydroxyapatite (HAp) derived from clamshell by impregnation with palladium (Pd) at different pH in wet precipitation...
This study focuses on the modification of natural hydroxyapatite (HAp) derived from clamshell by impregnation with palladium (Pd) at different pH in wet precipitation method to produce photoactive green materials for the degradation of synthetic dyes. It was found that, at pH 10, Pd has been successfully impregnated into HAp lattice with Ca/P ratio of 1.77 and particle distribution size range of 40-470 nm. The impregnation has resulted in the band gap of Pd/HAp at 3.19 eV, as calculated using Tauc's plot from the UV-Vis spectroscopy data of the Pd/HAp. Next, the photocatalytic activities of Pd/HAp were carried out with methyl oranges (MO) as models of water pollutants under UV irradiation. The photodegradation efficiency of the catalyst reached the optimum value of 41.63%, 48.17%, and 43.64% after 120 min of continuous UV irradiation for pH values 8, 10, and 11.5 of Pd/HAp samples, respectively. This study opens a new paradigm in using naturally derived materials as photocatalysts in the reduction of persistent water pollutants at a low cost and green sustainable approach.
PubMed: 37529225
DOI: 10.55730/1300-0527.3558 -
The Indian Journal of Medical Research Apr 2023In early stages of oral cancers, 20-40 per cent of cases have occult metastasis in cervical lymph nodes. Biologic imbalance between cellular proliferation and death...
BACKGROUND & OBJECTIVES
In early stages of oral cancers, 20-40 per cent of cases have occult metastasis in cervical lymph nodes. Biologic imbalance between cellular proliferation and death culminates in metastasis. The importance of cell cycle dysregulation in relation to lymph node involvement in oral squamous cell carcinoma (OSCC) has not been established yet. The aim was to determine the association between apoptotic bodies count and mitotic index in relation to regional lymph node involvement in OSCC.
METHODS
Thirty two methyl green-pyronin stained slides from paraffin-embedded sections of OSCC were evaluated for apoptotic bodies count and mitotic index in relation to regional lymph node involvement using light microscopy. Number of apoptotic bodies and mitotic figures were counted in 10 randomly selected hot spot areas (×400). Average count of apoptotic bodies and mitotic figures were determined and compared with regard to the presence/absence of lymph node involvement.
RESULTS
The count of apoptotic bodies in cases without metastasis to the regional lymph node was significantly higher than in cases with regional lymph node involvement. The mitotic index was not significantly different between groups in terms of regional lymph node involvement (P=0.24). No significant correlation was found between the apoptotic bodies count (r=-0.094, P=0.72) and mitotic index (r=-0.08, P=0.75) to the number of regional lymph nodes involved.
INTERPRETATION & CONCLUSIONS
Based on the results, it is suggested that apoptotic cell count can be a good parameter for showing the possibility of regional lymph node involvement in people with OSCC who do not have clinical symptoms of lymph node involvement.
Topics: Humans; Carcinoma, Squamous Cell; Squamous Cell Carcinoma of Head and Neck; Mouth Neoplasms; Head and Neck Neoplasms; Lymph Nodes
PubMed: 37282393
DOI: 10.4103/ijmr.IJMR_490_19 -
Frontiers in Chemistry 2021Dyes are one of the most hazardous chemicals causing significant environmental pollution and affecting water quality. Majority of the existing methods for dye removal...
Dyes are one of the most hazardous chemicals causing significant environmental pollution and affecting water quality. Majority of the existing methods for dye removal and degradation involve synthetic membranes and use of hazardous chemicals, further resulting in secondary pollution. The present study reports polysaccharide based novel composite hydrogel as biodegradable matrix for pH-responsive selective adsorption of cationic/anionic dyes. This membrane showed pH-responsive adsorption of methyl green (MG) and methyl orange (MO) with similar adsorption equilibrium, i.e., 315 and 276 mg g, respectively. Interestingly, selective adsorption at different pH has allowed separation of dye mixtures that holds incredible industrial importance for dyes recovery. The hydrogel matrix was able to completely separate MG, a model cationic dye at neutral pH from the dye mixture whereas, it was possible to remove 60% MO, a model anionic dye at acidic pH. Furthermore, comprehensive isothermal and kinetic studies of adsorption revealed that Freundlich isotherm describing the multilayer coverage and pseudo-second-order kinetics were followed. Thermodynamic studies indicated that the adsorption process was spontaneous and endothermic. In fact, the membrane was reusable for at least ten cycles and exhibited desorption efficiency of 80 and 60% for MO and MG, respectively, which may be further recycled to make the process environmentally sustainable. Overall, this study proposes an inexpensive, simple, biologically safe, and efficient adsorbent material for dye effluent treatment.
PubMed: 34778212
DOI: 10.3389/fchem.2021.761682 -
Annals of Diagnostic Pathology Aug 2020Accurate detection and quantification of hepatic fibrosis remain essential for assessing the severity of non-alcoholic fatty liver disease (NAFLD) and its response to...
Accurate detection and quantification of hepatic fibrosis remain essential for assessing the severity of non-alcoholic fatty liver disease (NAFLD) and its response to therapy in clinical practice and research studies. Our aim was to develop an integrated artificial intelligence-based automated tool to detect and quantify hepatic fibrosis and assess its architectural pattern in NAFLD liver biopsies. Digital images of the trichrome-stained slides of liver biopsies from patients with NAFLD and different severity of fibrosis were used. Two expert liver pathologists semi-quantitatively assessed the severity of fibrosis in these biopsies and using a web applet provided a total of 987 annotations of different fibrosis types for developing, training and testing supervised machine learning models to detect fibrosis. The collagen proportionate area (CPA) was measured and correlated with each of the pathologists semi-quantitative fibrosis scores. Models were created and tested to detect each of six potential fibrosis patterns. There was good to excellent correlation between CPA and the pathologist score of fibrosis stage. The coefficient of determination (R) of automated CPA with the pathologist stages ranged from 0.60 to 0.86. There was considerable overlap in the calculated CPA across different fibrosis stages. For identification of fibrosis patterns, the models areas under the receiver operator curve were 78.6% for detection of periportal fibrosis, 83.3% for pericellular fibrosis, 86.4% for portal fibrosis and >90% for detection of normal fibrosis, bridging fibrosis, and presence of nodule/cirrhosis. In conclusion, an integrated automated tool could accurately quantify hepatic fibrosis and determine its architectural patterns in NAFLD liver biopsies.
Topics: Artificial Intelligence; Automation; Azo Compounds; Biopsy; Clinical Trials as Topic; Collagen; Eosine Yellowish-(YS); Fibrosis; Humans; Image Processing, Computer-Assisted; Liver; Liver Cirrhosis; Methyl Green; Non-alcoholic Fatty Liver Disease; Organ Dysfunction Scores; Pathologists; Portal Vein; Practice Patterns, Physicians'; Severity of Illness Index; Supervised Machine Learning
PubMed: 32531442
DOI: 10.1016/j.anndiagpath.2020.151518 -
Turkiye Parazitolojii Dergisi Dec 2021This study aims to provide comparative information on the rumen ciliate fauna of goat () and sheep () living in Zawiya, Libya.
OBJECTIVE
This study aims to provide comparative information on the rumen ciliate fauna of goat () and sheep () living in Zawiya, Libya.
METHODS
We obtained rumen samples from 16 goats and 17 sheep after the slaughter in Zawiya, Libya between June and August 2016. We immediately fixed the well-mixed samples with an equal volume of 18.5% formalin. We filtered and stained the samples in the laboratory with methyl green formalin saline solution to determine the nuclei and added 2% Lugol's iodine solution to visualize the skeletal plates.
RESULTS
We found that the mean number (± standard deviation) of ciliates in the rumen contents from goats and sheep was 70.9±61.6×10 cells mL (minimum-maximum value, 4.0-187.0×10 cells mL) and 96.3±49.3×10 cells mL (minimum-maximum value, 19.5-235.0×10 cells mL), respectively. Results also showed that the total number of species per goat and sheep was 1-17 (mean, 8.2±4.7) and 1-13 (mean, 7.9±3.8), respectively. We identified 10 genera, 19 species, and 11 morphotypes in goats and 9 genera, 16 species, and 13 morphotypes in sheep. Additionally, we found that prevalence in all goats and sheep was 100%. On the other hand, we observed and in only one goat (6.3% prevalence) and in only one sheep (5.9% prevalence). Overall, the ruminal ciliate fauna of goat and sheep in Libya comprised species (mean for goats, 85.9%; mean for sheep, 83.5%).
CONCLUSION
This study recorded as a new endosymbiont in goats. To our knowledge, this study is the first to report all of the species detected in goats from Libya. Similarly, this is the first to detect , and in sheep from Libya.
Topics: Animals; Ciliophora; Goats; Laboratories; Libya; Rumen; Sheep
PubMed: 34889195
DOI: 10.4274/tpd.galenos.2021.39974 -
International Journal of Molecular... Feb 2021Ribosome-inactivating proteins (RIPs) are a class of cytotoxic enzymes that can inhibit protein translation by depurinating rRNA. Most plant RIPs are synthesized with a...
Ribosome-inactivating proteins (RIPs) are a class of cytotoxic enzymes that can inhibit protein translation by depurinating rRNA. Most plant RIPs are synthesized with a leader sequence that sequesters the proteins to a cell compartment away from the host ribosomes. However, several rice RIPs lack these signal peptides suggesting they reside in the cytosol in close proximity to the plant ribosomes. This paper aims to elucidate the physiological function of two nucleocytoplasmic RIPs from rice, in particular, the type 1 RIP referred to as OsRIP1 and a presumed type 3 RIP called nuRIP. Transgenic rice lines overexpressing these RIPs were constructed and studied for developmental effects resulting from this overexpression under greenhouse conditions. In addition, the performance of transgenic seedlings in response to drought, salt, abscisic acid and methyl jasmonate treatment was investigated. Results suggest that both RIPs can affect methyl jasmonate mediated stress responses.
Topics: Abscisic Acid; Acetates; Cyclopentanes; Cytosol; Gene Expression Regulation, Plant; Green Fluorescent Proteins; Oryza; Oxylipins; Phenotype; Plant Proteins; Plants, Genetically Modified; Protein Biosynthesis; Ribosomes; Salts; Saporins; Seedlings; Stress, Physiological
PubMed: 33535383
DOI: 10.3390/ijms22031434 -
Designed Monomers and Polymers 2022Recent years have witnessed an enormous development in photoinduced systems, opening up possibilities for advancements in industry and academia in terms of green...
Recent years have witnessed an enormous development in photoinduced systems, opening up possibilities for advancements in industry and academia in terms of green chemistry providing environmentally friendly conditions and spatiotemporal control over the reaction medium. A vast number of research have been conducted on photoinduced systems focusing on the development of new polymerization methods, although scarcely investigated, depolymerization of the synthesized polymers by photochemical means is also possible. Herein, we provide a comprehensive study of visible light induced dimanganese decacarbonyl (Mn(CO)) assisted depolymerization system for poly(methyl methacrylate) with chlorine chain end prepared by Atom Transfer Radical Polymerization. Contrary to the conventional procedures demanding high temperatures, the approach offers ambient temperature for the photodepolymerization process. This novel light-controlled concept is easily adaptable to macroscales and expected to promote further research in the fields matching with the environmental concerns.
PubMed: 36275914
DOI: 10.1080/15685551.2022.2135730 -
Materials (Basel, Switzerland) Feb 2023In this work, three types of ultrafiltration membranes with different characteristics (GR60PP, RC70PP and GR80PP) have been tested for the removal of the dye methyl...
In this work, three types of ultrafiltration membranes with different characteristics (GR60PP, RC70PP and GR80PP) have been tested for the removal of the dye methyl green. The tests were first carried out with the three membranes without any modification and then with the membranes' surfaces modified with reduced graphene oxide (rGO). The modification was achieved through physical treatment. The CR70PP membrane did not support the modification treatment and was discarded. The other membranes were initially characterized with distilled water tests to study the permeability to the solvent, and later, the permeate fluxes and the values of rejection coefficients were obtained at different working pressures with a fixed dye initial concentration. In addition, SEM images and SEM-EDX spectra of the native and modified membranes were obtained before and after the dye tests. The GR60PP membrane has shown the best results in relation to the modification because it has increased its rejection levels. On the opposite, the GR80PP membrane performs better without surface modification, achieving the highest rejection values and the highest permeate fluxes in its native form.
PubMed: 36836999
DOI: 10.3390/ma16041369 -
Journal, Genetic Engineering &... Dec 2016Today, discharge of hazardous dyes from textile industries in water bodies like lakes, rivers and groundwater has become a serious problem, which contributes to increase...
Today, discharge of hazardous dyes from textile industries in water bodies like lakes, rivers and groundwater has become a serious problem, which contributes to increase their pollution levels significantly. These pollutants are difficult to remove by traditional water treatment procedures. Thus, there is a need to develop more suitable methods of effluent treatment. Here, we describe use of green-synthesized nanostructured silver particles in degradation of hazardous dyes like Safranine O, Methyl red, Methyl orange and Methylene blue etc. The silver nanoparticles (AgNPs) used as nanocatalysts were synthesized using leaves. The reduction of silver ions and the formation of AgNPs have been assessed by UV-Vis spectroscopy. DLS, SEM-EDX, TEM, SAED and XRD studies revealed that the AgNPs were crystalline in nature with size range from 15 to 50 nm. The report emphasizes that the AgNPs are observed to be an excellent catalyst on reduction of hazardous dyes, which is confirmed by a decrease in absorbance maximum values.
PubMed: 30647629
DOI: 10.1016/j.jgeb.2016.09.005