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The Australasian Medical Journal 2011We describe a case of infective endocarditis due to Moraxella lacunata involving the native mitral and aortic valves, complicated by cerebral emboli and resultant...
We describe a case of infective endocarditis due to Moraxella lacunata involving the native mitral and aortic valves, complicated by cerebral emboli and resultant hemiparesis. The patient was treated with ceftriaxone and gentamicin and improved. This appears to be the first case reported in the medical literature of native multivalvular endocarditis produced by this rare organism.
PubMed: 23393524
DOI: 10.4066/AMJ.2011.718 -
Revista Argentina de Microbiologia 2023This is the first study of the genetic diversity of Moraxella spp. Isolates were detected in an Eye Hospital in the City of Buenos Aires. Due to the high frequency of...
This is the first study of the genetic diversity of Moraxella spp. Isolates were detected in an Eye Hospital in the City of Buenos Aires. Due to the high frequency of Moraxella spp. observed in corneal abscesses, we decided to validate their identification at the species level, determine their drug susceptibility and perform molecular subtyping. Seventeen (17) isolates obtained from corneal abscesses were evaluated. The identification was carried out using a combination of biochemical tests and MALDI-TOF mass spectrometry. Of these isolates, 88.2% were identified as Moraxella lacunata, and 11.8% as Moraxella nonliquefaciens. Molecular subtyping was performed using the pulsed-field gel electrophoresis (PFGE) technique. All isolates were typable and thirteen digestion patterns were identified. Based on the obtained results, the PFGE technique using the SmaI enzyme can be used for epidemiological studies of strains of these species.
Topics: Humans; Abscess; Moraxella; Electrophoresis, Gel, Pulsed-Field; Genetic Variation
PubMed: 35915013
DOI: 10.1016/j.ram.2022.03.004 -
Journal of Clinical Microbiology May 2014Our study is the first to compare the nasopharyngeal microbiota of pediatric pneumonia patients and control children by 454 pyrosequencing. A distinct microbiota was...
Our study is the first to compare the nasopharyngeal microbiota of pediatric pneumonia patients and control children by 454 pyrosequencing. A distinct microbiota was associated with different pneumonia etiologies. Viral pneumonia was associated with a high abundance of the operational taxonomic unit (OTU) corresponding to Moraxella lacunata. Patients with nonviral pneumonia showed high abundances of OTUs of three typical bacterial pathogens, Streptococcus pneumoniae complex, Haemophilus influenzae complex, and Moraxella catarrhalis. Patients classified as having no definitive etiology harbored microbiota particularly enriched in the H. influenzae complex. We did not observe a commensal taxon specifically associated with health. The microbiota of the healthy nasopharynx was more diverse and contained a wider range of less abundant taxa.
Topics: Adolescent; Case-Control Studies; Child; Child, Preschool; Haemophilus Infections; Haemophilus influenzae; Humans; Infant; Microbiota; Moraxella catarrhalis; Moraxellaceae Infections; Nasopharynx; Pneumococcal Infections; Pneumonia; Prospective Studies; Respiratory Tract Infections; Streptococcus pneumoniae
PubMed: 24599973
DOI: 10.1128/JCM.03280-13 -
The Journal of Biological Chemistry Apr 1999The recombinase, Piv, is essential for site-specific DNA inversion of the type IV pilin DNA segment in Moraxella lacunata and Moraxella bovis. Piv shows significant...
The recombinase, Piv, is essential for site-specific DNA inversion of the type IV pilin DNA segment in Moraxella lacunata and Moraxella bovis. Piv shows significant homology with the transposases of the IS110/IS492 family of insertion elements, but, surprisingly, Piv contains none of the conserved amino acid motifs of the lambda Int or Hin/Res families of site-specific recombinases. Therefore, Piv may mediate site-specific recombination by a novel mechanism. To begin to determine how Piv may assemble a synaptic nucleoprotein structure for DNA cleavage and strand exchange, we have characterized the interaction of Piv with the DNA inversion region of M. lacunata. Gel shift and nuclease/chemical protection assays, competition and dissociation rate analyses, and cooperativity studies indicate that Piv binds two distinct recognition sequences. One recognition sequence, found at multiple sites within and outside of the invertible segment, is bound by Piv protomers with high affinity. The second recognition sequence is located at the recombination cross-over sites at the ends of the invertible element; Piv interacts with this sequence as an oligomer with apparent low affinity. A model is proposed for the role of the different Piv binding sites of the M. lacunata inversion region in the formation of an active synaptosome.
Topics: Base Sequence; Chromosome Inversion; DNA; DNA Nucleotidyltransferases; Integrases; Molecular Sequence Data; Molecular Weight; Moraxella; Oligonucleotides; Recombinases; Recombination, Genetic; Substrate Specificity
PubMed: 10092658
DOI: 10.1074/jbc.274.14.9698 -
Journal of the Royal Society of Medicine Oct 1991
Topics: Aged; Arthritis, Infectious; Humans; Kidney Failure, Chronic; Leukocytosis; Male; Moraxella; Shoulder Joint; Vasculitis
PubMed: 1744856
DOI: 10.1177/014107689108401027 -
Journal of Bacteriology Apr 1997A plasmid library of Neisseria gonorrhoeae sequences was screened for the ability to mediate recombinations on a sequence containing the Moraxella lacunata type 4 pilin...
A plasmid library of Neisseria gonorrhoeae sequences was screened for the ability to mediate recombinations on a sequence containing the Moraxella lacunata type 4 pilin gene invertible region in Escherichia coli. A plasmid containing the N. gonorrhoeae sequence encoding the putative recombinase (gcr) was identified and sequenced. Plasmids containing gcr were able to mediate site-specific recombinations despite a weak amino acid homology to Piv, the native M. lacunata pilin gene invertase. The gcr gene is present only in pathogenic strains of Neisseria tested; however, in our assays gene knockouts of gcr did not alter the variation of surface features that play a role in the pathogenesis of N. gonorrhoeae.
Topics: Amino Acid Sequence; Bacterial Outer Membrane Proteins; Bacterial Proteins; Base Sequence; Cloning, Molecular; DNA Nucleotidyltransferases; DNA, Bacterial; Fimbriae Proteins; Gene Expression Regulation, Bacterial; Genes, Bacterial; Molecular Sequence Data; Moraxella; Neisseria gonorrhoeae; Promoter Regions, Genetic; Recombination, Genetic; Species Specificity
PubMed: 9079926
DOI: 10.1128/jb.179.7.2382-2388.1997 -
Journal of Clinical Microbiology Nov 2009Moraxella lacunata is a rare, usually commensal gram-negative rod most commonly associated with eye infections. We report a unique case of noniatrogenic M. lacunata...
Moraxella lacunata is a rare, usually commensal gram-negative rod most commonly associated with eye infections. We report a unique case of noniatrogenic M. lacunata bacteremia and septic knee arthritis in a patient with class III-IV lupus nephritis and speculate on the association between invasive Moraxella infection and renal impairment.
Topics: Anti-Bacterial Agents; Arthritis, Infectious; Bacteremia; Bacterial Typing Techniques; Debridement; Female; Humans; Infusions, Intravenous; Lupus Nephritis; Moraxella; Moraxellaceae Infections; Penicillanic Acid; Piperacillin; Piperacillin, Tazobactam Drug Combination; Treatment Outcome; Young Adult
PubMed: 19794049
DOI: 10.1128/JCM.01403-09 -
Journal of Bacteriology Jul 1994Deletion analysis of the subcloned DNA inversion region of Moraxella lacunata indicates that Piv is the only M. lacunata-encoded factor required for site-specific...
Amino acid sequence homology between Piv, an essential protein in site-specific DNA inversion in Moraxella lacunata, and transposases of an unusual family of insertion elements.
Deletion analysis of the subcloned DNA inversion region of Moraxella lacunata indicates that Piv is the only M. lacunata-encoded factor required for site-specific inversion of the tfpQ/tfpI pilin segment. The predicted amino acid sequence of Piv shows significant homology solely with the transposases/integrases of a family of insertion sequence elements, suggesting that Piv is a novel site-specific recombinase.
Topics: Amino Acid Sequence; Base Sequence; Chromosome Inversion; DNA Nucleotidyltransferases; DNA Transposable Elements; Integrases; Models, Genetic; Molecular Sequence Data; Moraxella; Nucleotidyltransferases; Recombinases; Sequence Homology, Amino Acid; Transposases
PubMed: 8021196
DOI: 10.1128/jb.176.13.4160-4164.1994 -
Journal of Clinical Microbiology Mar 2001Fast and reliable identification of microbial isolates is a fundamental goal of clinical microbiology. However, in the case of some fastidious gram-negative bacterial...
Fast and reliable identification of microbial isolates is a fundamental goal of clinical microbiology. However, in the case of some fastidious gram-negative bacterial species, classical phenotype identification based on either metabolic, enzymatic, or serological methods is difficult, time-consuming, and/or inadequate. 16S or 23S ribosomal DNA (rDNA) bacterial sequencing will most often result in accurate speciation of isolates. Therefore, the objective of this study was to find a hypervariable rDNA stretch, flanked by strongly conserved regions, which is suitable for molecular species identification of members of the Neisseriaceae and Moraxellaceae. The inter- and intrageneric relationships were investigated using comparative sequence analysis of PCR-amplified partial 16S and 23S rDNAs from a total of 94 strains. When compared to the type species of the genera Acinetobacter, Moraxella, and Neisseria, an average of 30 polymorphic positions was observed within the partial 16S rDNA investigated (corresponding to Escherichia coli positions 54 to 510) for each species and an average of 11 polymorphic positions was observed within the 202 nucleotides of the 23S rDNA gene (positions 1400 to 1600). Neisseria macacae and Neisseria mucosa subsp. mucosa (ATCC 19696) had identical 16S and 23S rDNA sequences. Species clusters were heterogeneous in both genes in the case of Acinetobacter lwoffii, Moraxella lacunata, and N. mucosa. Neisseria meningitidis isolates failed to cluster only in the 23S rDNA subset. Our data showed that the 16S rDNA region is more suitable than the partial 23S rDNA for the molecular diagnosis of Neisseriaceae and Moraxellaceae and that a reference database should include more than one strain of each species. All sequence chromatograms and taxonomic and disease-related information are available as part of our ribosomal differentiation of medical microorganisms (RIDOM) web-based service (http://www.ridom.hygiene.uni-wuerzburg.de/). Users can submit a sequence and conduct a similarity search against the RIDOM reference database for microbial identification purposes.
Topics: DNA, Ribosomal; Databases, Factual; Genes, rRNA; Humans; Internet; Molecular Sequence Data; Moraxella; Neisseriaceae; Neisseriaceae Infections; Phylogeny; RNA, Ribosomal, 16S; RNA, Ribosomal, 23S; Sequence Analysis, DNA
PubMed: 11230407
DOI: 10.1128/JCM.39.3.936-942.2001 -
Scandinavian Journal of Infectious... 2007We describe the first case of Moraxella lacunata definite native valve endocarditis in a patient with previously normal mitral valves. The disease was complicated with...
We describe the first case of Moraxella lacunata definite native valve endocarditis in a patient with previously normal mitral valves. The disease was complicated with embolizations of the brain and spleen. After 6 weeks of antimicrobial treatment, valvular replacement was performed. The clinical course and diagnostic findings suggest that Moraxella lacunata possesses high aggressiveness leading to progressive valvular destruction and embolizations.
Topics: Endocarditis, Bacterial; Female; Heart Valve Diseases; Heart Valve Prosthesis Implantation; Humans; Intracranial Embolism; Middle Aged; Mitral Valve; Moraxella; Moraxellaceae Infections
PubMed: 17852895
DOI: 10.1080/00365540701403002