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Plants (Basel, Switzerland) Nov 2022The aim of our research was to study the endosphere of four halophytic plants: L., (R.Br.), (Pall.) and (L.) Schrad. from arid and saline areas of the Stavropol...
The aim of our research was to study the endosphere of four halophytic plants: L., (R.Br.), (Pall.) and (L.) Schrad. from arid and saline areas of the Stavropol Territory, Russia. In total, 28 endophyte strains were isolated from the roots and stems of these halophytic plants. Most of the isolates (23 out of 28) were identified as sp. while others belonged to the genera and . Three strains of sp. (Se5R, Se1-1R, and Se1-3S), isolated from the were capable of growth at 55 °C and in 10% of NaCl. Strains Se1-4S, Kp20-2S, and Bs11-2S sp. (isolated from the , and respectively) demonstrated strong plant growth promoting activity: 85-265% over control lettuce plants and a high degree of growth suppression (59.1-81.2%) of pathogenic fungi , and . Selected strains can be promising candidates for the development of bioinoculants to facilitate salt soil phytoremediation and be beneficial for mitigating the salt stress to the plants growing in salt-affected habitats.
PubMed: 36365445
DOI: 10.3390/plants11212992 -
Current Microbiology Jul 2023The polymicrobial nature of diabetic foot infection (DFI) makes accurate identification of the DFI microbiota, including rapid detection of drug resistance, challenging....
The polymicrobial nature of diabetic foot infection (DFI) makes accurate identification of the DFI microbiota, including rapid detection of drug resistance, challenging. Therefore, the main objective of this study was to apply matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI TOF MS) technique accompanied by multiply culture conditions to determine the microbial patterns of DFIs, as well as to assess the occurrence of drug resistance among Gram-negative bacterial isolates considered a significant cause of the multidrug resistance spread. Furthermore, the results were compared with those obtained using molecular techniques (16S rDNA sequencing, multiplex PCR targeting drug resistance genes) and conventional antibiotic resistance detection methods (Etest strips). The applied MALDI-based method revealed that, by far, most of the infections were polymicrobial (97%) and involved many Gram-positive and -negative bacterial species-19 genera and 16 families in total, mostly Enterobacteriaceae (24.3%), Staphylococcaceae (20.7%), and Enterococcaceae (19.8%). MALDI drug-resistance assay was characterized by higher rate of extended-spectrum beta-lactamases (ESBLs) and carbapenemases producers compared to the reference methods (respectively 31% and 10% compared to 21% and 2%) and revealed that both the incidence of drug resistance and the species composition of DFI were dependent on the antibiotic therapy used. MALDI approach included antibiotic resistance assay and multiply culture conditions provides microbial identification at the level of DNA sequencing, allow isolation of both common (eg. Enterococcus faecalis) and rare (such as Myroides odoratimimus) bacterial species, and is effective in detecting antibiotic-resistance, especially those of particular interest-ESBLs and carbapenemases.
Topics: Humans; Diabetic Foot; Enterobacteriaceae; Anti-Bacterial Agents; Enterobacteriaceae Infections; Gram-Negative Bacteria; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Diabetes Mellitus
PubMed: 37405539
DOI: 10.1007/s00284-023-03384-z -
Foods (Basel, Switzerland) Jun 2023In this study, the quality (sensory evaluation, microbial enumerate, color, tvb-n (total volatile basic nitrogen), tca-soluble peptide (trichloroacetic acid-soluble...
In this study, the quality (sensory evaluation, microbial enumerate, color, tvb-n (total volatile basic nitrogen), tca-soluble peptide (trichloroacetic acid-soluble peptide), muscle glucose, lactate, total sugar, Bas (Biogenic amines), VOCs (volatile organic compounds) and the microbial dynamic structure in samples stored at 4 °C were evaluated, and the relationship between VOCs and the diversity structure of microorganisms was also discussed. It was determined by sensory evaluation that the shelf life of samples was around 8 days. Protein and sugar were detected in large quantities by microorganisms in the later stage. At the same time, this also caused a large amount of Bas (biogenic amines) (tyramine, cadaverine, and putrescine). According to high-throughput amplicon sequencing, the initial microbiota of samples was mainly composed of , , , , , , , and . Among them, was the most advantageous taxon of samples at the end of the shelf life. The minor fraction of the microbial consortium consisting of , and was detected. The substances 3-methyl-1-butanol, ethyl acetate, and acetone were the main volatile components. The glucose, lactic acid, and total sugar were negatively correlated with , , , and ; the proportion of these microorganisms was relatively high in the early stage. TVB-N and TCA-soluble peptides were positively correlated with , , , , and , and these microorganisms increased greatly in the later stage. The substance 3-methyl-1-butanol was positively correlated with and negatively correlated with . Ethyl acetate was associated with , and . Acetone was positively correlated with .
PubMed: 37444217
DOI: 10.3390/foods12132479 -
Cureus Sep 2023spp.-induced cutaneous infections are rare, with only 17 reported cases in the literature. spp. behave like low-grade opportunistic pathogens, with symptomatic...
spp.-induced cutaneous infections are rare, with only 17 reported cases in the literature. spp. behave like low-grade opportunistic pathogens, with symptomatic infections observed typically in severely immunocompromised patients and seldom in immunocompetent patients. In this paper, we present an immunocompetent 61-year old male with a past medical history of hypertension, hyperlipidemia, morbid obesity, and patient-reported peripheral neuropathy who presented to the transitional care clinic with bilateral lower extremity swelling and hemosiderin-pigmented dry wounds consistent with diagnosis of chronic venous stasis dermatitis with resolved secondary infection. Further literature review about spp. and its resistance mechanism, antibiotic susceptibility, and biofilm production are also included in this paper.
PubMed: 37846255
DOI: 10.7759/cureus.45319 -
Scientific Reports Jul 2022The housefly (Musca domestica) is a wide-ranging insect, often associated with decaying matter from livestock and humans. The septic environments in which houseflies...
The housefly (Musca domestica) is a wide-ranging insect, often associated with decaying matter from livestock and humans. The septic environments in which houseflies live are believed to be a rich source for microbial acquisition. Although the housefly can harbor a wide range of microorganisms, it is not yet well known which microbes are always recurrent, which are dispensable and which environmentally dependent. In the present study, we aim at identifying which microbes are recurrently associated with the housefly gut throughout the species' life cycle and whether their acquisition relies on the fly's living environment. We surveyed three housefly strains-two of them kept under standard laboratory conditions for a long time and one wild-caught. To track any shifts happening throughout the lifecycle of the housefly and to test the consistency of the revealed microbial communities, we sampled houseflies at five developmental stages over the course of four consecutive generations. Both the bacterial and fungal microbiota of five developmental stages were studied for all samples, using amplicon sequencing for the 16S and ITS1 rRNA gene, respectively. Results revealed diverse microbial communities yet consistent for each of the two distinct sampling environments. The wild-caught population showed a more diverse and more distinct gut microbiota than the two laboratory strains, even though the strain was phylogenetically similar and shared geographic origin with one of them. Two bacterial genera, Myroides and Providencia, and two yeasts, Trichosporon and Candida tropicalis, were present in all sampled larvae and pupae, regardless of the strain. Analysis of the provided diet revealed that the flies acquired the yeasts through feeding. Our main findings show that houseflies might lose microbial diversity when reared in controlled environments, however they can maintain a consistent set of bacteria. We conclude that although the environment can facilitate certain microbial transmission routes for the housefly, and despite the fungal microbiota being largely acquired through diet, the larval bacterial gut microbiome remains relatively consistent within the same developmental stage.
Topics: Animals; Bacteria; Gastrointestinal Microbiome; Houseflies; Larva; Microbiota
PubMed: 35778448
DOI: 10.1038/s41598-022-15186-7 -
Revista Chilena de Infectologia :... Apr 2021Infections due to Gram-negative bacteria of the genus Myroides are very rare and generally affect the skin and soft tissues of patients with some degree of...
Infections due to Gram-negative bacteria of the genus Myroides are very rare and generally affect the skin and soft tissues of patients with some degree of immunocompromise. We present a case of a 23-year-old patient with a history of myelomeningocele surgically resolved at 3 years of age and bot foot, who presented with a deep infection of the right lower extremity by Myroides odoratimimus. The species identification was carried out with MALDI-TOF and the treatment was initially carried out with meropenem and finally then ciprofloxacin, in addition to right supramaleolar amputation.
Topics: Adult; Flavobacteriaceae; Flavobacteriaceae Infections; Humans; Osteomyelitis; Soft Tissue Infections; Young Adult
PubMed: 34184722
DOI: 10.4067/S0716-10182021000200297 -
Microorganisms Nov 2023The intestinal bacteria of insects are crucial to the growth and development of the host. It has been found that various physiological processes of insects, such as...
The intestinal bacteria of insects are crucial to the growth and development of the host. It has been found that various physiological processes of insects, such as immune response, metabolism, reproductive ability, and growth and development, involve the gastrointestinal flora. However, many external factors affect the composition of insects' intestinal microorganisms, such as the type of dietary substrate. (Robineau-Desvoidy, 1830) (Diptera: Sarcophagidae) is of great significance in medicine and forensic science. In this study, we investigated the effects of ciprofloxacin on the growth and gut microbiota of . The results demonstrated that the maximum body length of larvae was not affected by ciprofloxacin, while the growth rate of body length quickened as the concentration of the drug increased. The weight of the pupa and adult was reduced significantly due to the effect of ciprofloxacin. After analyzing the gut microbiota composition of in different drug groups, it was indicated that , , , , , and play important roles in the growth of . However, they still need to be further studied. In general, ciprofloxacin can affect the gut microbial community structure, which in turn affects the fitness of the host.
PubMed: 38138011
DOI: 10.3390/microorganisms11122867 -
Insects Feb 2022We sequenced the complete mitogenomes, 18S and 28S rRNA of two new isolates, collected in Eastern Nebraska and Northern Arkansas (US). Based on molecular sequence data...
We sequenced the complete mitogenomes, 18S and 28S rRNA of two new isolates, collected in Eastern Nebraska and Northern Arkansas (US). Based on molecular sequence data comparison and morphological characteristics, the new isolates were identified as a subspecies of Fabricius 1775, for which we propose the subspecies names '' and ''. The new 18S and 28S rRNA sequences were found to be 99% and 98% identical to . There are no other 18S or 28S rRNA sequences in the Genbank database, however, phylogenetic analysis of the Cox1 genes showed Chaudoir, 1843, and Morawitz, 1863, as the closest relatives. This is the first report of a mitogenome for , and only the second mitogenome for that genus. The nucleotide sequence identity between the mitogenomes of the two isolates is 98.8%, while the earlier sequenced Forster 1771 mitogenome is more distantly related, with only 90% (to ssp. ) and 89% (to ssp. ) overall nucleotide sequence identity. These new mitogenomes, and their phylogenetic analysis, firmly establish the position of on the Carabidae family tree and further refine the genus. In addition to the molecular data provided for the species, this approach also allowed us to identify bacterial and viral signatures for , , , and a giant virus, associated with the species. We hereby present a simple and efficient protocol for identification and phylogenetic analysis of , that is applicable to other Coleoptera, based on total DNA extraction and Illumina short-read Next-Gen sequencing.
PubMed: 35206763
DOI: 10.3390/insects13020190 -
Infection and Drug Resistance 2020Catheter-related infection (CRI) is one of the most frequent causes of hospitalizations for immunocompromised patients. A major challenge is the increased prevalence of...
PURPOSE
Catheter-related infection (CRI) is one of the most frequent causes of hospitalizations for immunocompromised patients. A major challenge is the increased prevalence of . The purpose of the present study was to evaluate the clinical features and molecular characteristics of collected from a general hospital in Shanghai, China.
PATIENTS AND METHODS
From July 2015 to August 2016, a total of 22 isolates of were collected from inpatients respectively from the biliary and pancreatic surgery (6/22) and the urology department (16/22). Clonal relatedness among the isolates was assessed using pulsed-field gel electrophoresis (PFGE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Moreover, the antimicrobial susceptibility tests were carried out using the Clinical and Laboratory Standards Institute (CLSI) broth microdilution method. The presence of antibiotic resistance genes was screened using the polymerase chain reaction (PCR) assay. Additionally, protein structure prediction was analyzed using PSIPRED and RaptorX.
RESULTS
PFGE differentiated these isolates into six possibly related clones from two different departments obtained during a distinct period, indicating clonal dissemination in the two departments. We compared the dendrograms of isolates obtained by MALDI-TOF MS with those obtained by PFGE and found that the coincidence rate between them was only 68.2%. All the isolates were highly resistant to most available antibiotics, including carbapenems. Furthermore, chromosome-encoded β-lactamases MUS-1 was confirmed by PCR in 6 of 22 isolates. Herein, we also reported a novel variant of in the remaining 16 isolates, which encodes MUS-3 protein at position 60 (Valine to Alanine), differing from the structure of MUS-1.
CONCLUSION
The opportunistic and extensively antibiotic-resistant has a small range of epidemics in these two different departments. Clinicians should be aware that may induce a severe nosocomial outbreak of catheter-related infections, particularly in immunocompromised patients.
PubMed: 32612373
DOI: 10.2147/IDR.S251626 -
The Brazilian Journal of Infectious... 2012Members of the genus Myroides are aerobic Gram-negative bacteria that are common in environmental sources, but are not components of the normal human microflora.... (Review)
Review
Members of the genus Myroides are aerobic Gram-negative bacteria that are common in environmental sources, but are not components of the normal human microflora. Myroides organisms behave as low-grade opportunistic pathogens, causing infections in severely immunocompromised patients and rarely, in immunocompetent hosts. A case of Myroides odoratimimus cellulitis following a pig bite in an immunocompetent child is presented, and the medical literature on Myroides spp. soft tissue infections is reviewed.
Topics: Adolescent; Animals; Bites and Stings; Cellulitis; Flavobacteriaceae; Flavobacteriaceae Infections; Humans; Immunocompetence; Male; Soft Tissue Infections; Swine
PubMed: 22846131
DOI: 10.1016/j.bjid.2012.06.004