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Frontiers in Microbiology 2020The inhibitory properties of novel antimicrobial proteins against food-borne pathogens such as offer extensive benefits to the food and medical industries. In this...
The inhibitory properties of novel antimicrobial proteins against food-borne pathogens such as offer extensive benefits to the food and medical industries. In this study, we have identified antimicrobial proteins from a milk curd-derived bacterial isolate that exhibits antilisterial activity using genome mining and mass spectrometry analysis. The analysis of the draft genome sequence identified the isolate as Kp10, and predicted the presence of antimicrobial paenibacillin, paenilan, paeninodin, sactipeptides, thiazole-oxazole modified microcin, and histone-like DNA binding protein HU encoded in its genome. Interestingly, nanoLC-MS/MS analysis identified two histone-like DNA binding proteins HU as predicted earlier, exhibiting antilisterial activity. Additionally, translation initiation factor IF-1 and 50S ribosomal protein L29 were also discovered by the mass spectrometry in the active fractions. The antilisterial activity of the four proteins was verified through heterologous protein expression and antimicrobial activity assay . This study has identified structural regulatory proteins from possessing antilisterial activity with potential future application in the food and medical industries.
PubMed: 32714281
DOI: 10.3389/fmicb.2020.00960 -
Heliyon Mar 2023Barley scald is very important in temperate and wet regions worldwide and has become one of the most important foliar diseases. Before the development of recent... (Review)
Review
Barley scald is very important in temperate and wet regions worldwide and has become one of the most important foliar diseases. Before the development of recent technologies, several scientists had argued that is the causal agent of scald disease. However, the causal agent of this disease was revised and recognized as . Again recently, was suggested to be replaced as the causal agent of . The disease outbreak is depending on cool and frequent rainfall. Because of scald disease significance, numerous management practices have been advocated. Then, resistance materials, and mixing of resistant and susceptible cultivars have been used as the best management methods. Several studies have demonstrated that some cultivars and landraces of barley are resistant to scald disease during the seedling and adult growth stages. The first cultivar is "Atlas 46″ which was created from the cultivar "Turk". From biological method: , KaI245, and are very effective in treating this disease. Finally, as a last option, different fungicides have been suggested. Pathogenicity testing, seed treatments, tillage, cultivar mixtures, and biological control are all commonly overlooked in developing countries. Cultural practices such as times of fungicide application, appropriate time of sowing to scape disease, and tillage practices which are adopted for other diseases are greatly missed for scald disease. Then, we are intended to assess the various findings available on barley scald biology, taxonomy, and management.
PubMed: 36938428
DOI: 10.1016/j.heliyon.2023.e14315 -
Molecules (Basel, Switzerland) Oct 2018Pectate lyase (EC 4.2.2.2) catalyzes the cleavage of α-1,4-glycosidic bonds of pectin polymers, and it has potential uses in the textile industry. In this study, a...
Pectate lyase (EC 4.2.2.2) catalyzes the cleavage of α-1,4-glycosidic bonds of pectin polymers, and it has potential uses in the textile industry. In this study, a novel pectate lyase belonging to polysaccharide lyase family 10 was screened from the secreted enzyme extract of KF-1 and identified by liquid chromatography-MS/MS. The gene was cloned from KF-1 genomic DNA and expressed in . The recombinant enzyme PpPel10a had a predicted Mr of 45.2 kDa and I of 9.41. Using polygalacturonic acid (PGA) as substrate, the optimal conditions for PpPel10a reaction were determined to be 50 °C and pH 9.0, respectively. The K, v and k values of PpPel10a with PGA as substrate were 0.12 g/L, 289 μmol/min/mg, and 202.3 s, respectively. Recombinant PpPel10a degraded citrus pectin, producing unsaturated mono- and oligogalacturonic acids. PpPel10a reduced the viscosity of PGA, and weight loss of ramie () fibers was observed after treatment with the enzyme alone (22.5%) or the enzyme in combination with alkali (26.3%). This enzyme has potential for use in plant fiber processing.
Topics: Amino Acid Sequence; Chromatography, Liquid; Cloning, Molecular; Enzyme Activation; Gene Expression; Paenibacillus polymyxa; Pectins; Polysaccharide-Lyases; Proteolysis; Recombinant Proteins; Sequence Analysis, DNA; Substrate Specificity; Tandem Mass Spectrometry
PubMed: 30373112
DOI: 10.3390/molecules23112774 -
Journal of Food Protection Jan 2005We evaluated anti-Campylobacter activity among 365 Bacillus and Paenibacillus isolates from poultry production environments. One novel antagonistic Bacillus circulans...
We evaluated anti-Campylobacter activity among 365 Bacillus and Paenibacillus isolates from poultry production environments. One novel antagonistic Bacillus circulans and three Paenibacillus polymyxa strains were identified and further studied. Cell-free ammonium sulfate precipitate (crude antimicrobial preparation) was obtained from each candidate culture. Zones of Campylobacter growth inhibition surrounding 10 microl of this crude antimicrobial preparation were quantified using a spot test. Campylobacter growth resumed when the preparation was preincubated with selected protease enzymes, demonstrating peptide characteristics consistent with a bacteriocin. These peptides were further purified using combinations of molecular mass resolution and ion exchange chromatography. Molecular masses of the peptides were estimated at approximately 3,500 Da by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Isoelectric focusing was used to determine the pI values of the peptides. Amino acid sequences of the bacteriocins and more precise molecular masses were obtained by matrix-assisted laser desorption and ionization-time of flight (MALDI-TOF) analysis. The bacteriocin from P. polymyxa NRRL B-30507 had a pI of 4.8, that from P. polymyxa NRRL B-30509 had a pI of 7.2, that from P. polymyxa NRRL B-30508 had a pI of 4.8, and that from B. circulans NRRL B-30644 had a pI of 7.8. The amino acid sequences were consistent with those of class IIa bacteriocins. These antagonists and the corresponding bacteriocins may be useful in the control of Campylobacter infection in poultry.
Topics: Amino Acid Sequence; Animals; Bacillus; Bacterial Proteins; Bacteriocins; Campylobacter Infections; Campylobacter jejuni; Colony Count, Microbial; Electrophoresis, Polyacrylamide Gel; Isoelectric Focusing; Molecular Weight; Poultry; Poultry Diseases; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
PubMed: 15690798
DOI: 10.4315/0362-028x-68.1.11 -
Data in Brief Dec 2018We present the data corresponding to the ultrastructural characteristics of isolates and control efficacy of ShX301 for controlling Verticillium wilt of cotton,...
We present the data corresponding to the ultrastructural characteristics of isolates and control efficacy of ShX301 for controlling Verticillium wilt of cotton, isolated in experimental fields at the Sanyuan Agricultural Experiment Station of North-West Agriculture and Forestry University, Sanyuan county, Shaanxi province, China. Ultrastructural characteristics of isolates made using technique of transmission electron microscopy. A strain ShX301 has a broad-spectrum antifungal activity against and other plant pathogens and has been used for experiments for controlling this disease in greenhouse, "Biocontrol potential of against infecting cotton plants" [1].
PubMed: 30364525
DOI: 10.1016/j.dib.2018.09.058 -
Frontiers in Microbiology 2023Pitaya canker, caused by , is one of the most important fungal diseases that cause significant losses in production. To replace chemical pesticides, the use of...
Pitaya canker, caused by , is one of the most important fungal diseases that cause significant losses in production. To replace chemical pesticides, the use of biocontrol strains to manage plant diseases has been the focus of research. In this study, the bacterial strain AF01, identified as , exhibited significant antifungal effects against and four other pitaya fungal pathogens. The strain AF01 produces 13 fusaricidins, which directly inhibit mycelial growth, spore germination and germ tube elongation by causing the membrane integrity and cell ultrastructure to incur irreversible damage. Pot experiment and yield test confirmed that AF01 provided preservative effects by reducing the disease index. In comparison to the untreated control groups, RNA-seq data showed that AF01 selectively blocked some transcription and translation processes and inhibited RNA and DNA structural dynamics, energy production and conversion, and signal transduction, particularly cell wall biosynthesis, changes in membrane permeability, and impairment of protein biosynthesis. Thus, AF01 could be potentially useful as a suitable biocontrol agent for pitaya canker.
PubMed: 37266020
DOI: 10.3389/fmicb.2023.1188722 -
Microbiology (Reading, England) Jul 2023Over the past decades, antibiotic resistance has become a major clinical problem, and searching for new therapeutic strategies seems to be necessary. Using novel natural...
Over the past decades, antibiotic resistance has become a major clinical problem, and searching for new therapeutic strategies seems to be necessary. Using novel natural compounds, antimicrobial peptides, and bacteriophages is the most promising solution. In this study, various cationic metabolite-producer bacteria were isolated from different soil samples. Two isolates were identified as HS4 (accession number: MW791428) and HS5 (accession number: MW791430) based on biochemical characteristics and phylogenetic analysis using 16S rRNA gene sequences. The cationic compound in the fermentation broth was precipitated and purified with sodium tetraphenylborate salt. The purified cationic peptide was confirmed to be epsilon-poly-l-lysine by structural and molecular analysis using High-Performance Liquid Chromatography, Sodium dodecyl-sulfate-polyacrylamide gel electrophoresis, and Fourier-transform infrared spectroscopy. The antibacterial activity of epsilon-poly-l-lysine was evaluated against ATCC 25923, ATCC 25922, ATCC 29212, ATCC 13880, and ATCC 13883 by microdilution method. Furthermore, the antibacterial effects of purified epsilon-poly-l-lysine in combination with two long non-contractile tail bacteriophages against vancomycin-resistant and colistin-resistant were investigated. The results indicated great antibacterial activity of epsilon-poly-l-lysine which was produced by two novel bacteria. The epsilon-poly-l-lysine as a potent cationic antimicrobial peptide is demonstrated to possess great antimicrobial activity against pathogenic and also antibiotic-resistant bacteria.
Topics: Polylysine; Stenotrophomonas maltophilia; Paenibacillus polymyxa; RNA, Ribosomal, 16S; Phylogeny; Anti-Bacterial Agents; Bacteria; Antimicrobial Cationic Peptides; Microbial Sensitivity Tests
PubMed: 37477972
DOI: 10.1099/mic.0.001363 -
Journal of Applied Microbiology Aug 2009To screen and evaluate the biocontrol potential of Paenibacillus strains against disease complex caused by Meloidogyne incognita and Fusarium oxysporum f. sp....
AIM
To screen and evaluate the biocontrol potential of Paenibacillus strains against disease complex caused by Meloidogyne incognita and Fusarium oxysporum f. sp. lycopersici interactions.
METHODS AND RESULTS
Paenibacillus strains were collected from rotten ginseng roots. The strains were tested under in vitro and pots for their inhibitory activities, and biocontrol potential against disease complex caused by M. incognita and F. oxysporum f. sp. lycopersici on tomato. In in vitro experiments, among 40 tested strains of Paenibacillus spp., 11 strains showed antifungal and nematicidal activities against F. oxysporum f. sp. lycopersici and M. incognita, respectively. Paenibacillus polymyxa GBR-462; GBR-508 and P. lentimorbus GBR-158 showed the strongest antifungal and nematicidal activities. These three strains used in pot experiment reduced the symptom development of the disease complex (wilting and plant death), and increased plant growth. The control effects were estimated to be 90-98%, and also reduced root gall formation by 64-88% compared to the untreated control.
CONCLUSION
The protective properties of selected Paenibacillus strains make them as potential tool to reduce deleterious impact of disease complex plants.
SIGNIFICANCE AND IMPACT OF THE STUDY
The study highlights biocontrol potential of Paenibacillus strains in management of disease complex caused by nematode-fungus interaction.
Topics: Animals; Antibiosis; Fusarium; Hyphae; Lethal Dose 50; Solanum lycopersicum; Microscopy, Electron, Scanning; Paenibacillus; Pest Control, Biological; Plant Diseases; Plant Growth Regulators; Plant Roots; Tylenchoidea
PubMed: 19457027
DOI: 10.1111/j.1365-2672.2009.04238.x -
Microbiology Resource Announcements Nov 2023Here, we report 10 distinct bacterial genomes from Amazonian dark earths, including six identified as , while the remaining four were unique representatives of , , , and...
Here, we report 10 distinct bacterial genomes from Amazonian dark earths, including six identified as , while the remaining four were unique representatives of , , , and sp., respectively. Each strain exhibited antagonistic activity against , underscoring their potential as sustainable agriculture resources.
PubMed: 37811974
DOI: 10.1128/MRA.00574-23 -
Frontiers in Microbiology 2021Genome-based identification of new antibiotics is emerging as an alternative to traditional methods. However, uncovering hidden antibiotics under the background of known...
Genome-based identification of new antibiotics is emerging as an alternative to traditional methods. However, uncovering hidden antibiotics under the background of known antibiotics remains a challenge. To over this problem using a quick and effective genetic approach, we developed a multiplex genome editing system using a cytosine base editor (CBE). The CBE system achieved simultaneous double, triple, quadruple, and quintuple gene editing with efficiencies of 100, 100, 83, and 75%, respectively, as well as the 100% editing efficiency of single targets in . Whole-genome sequencing of the edited strains showed that they had an average of 8.5 off-target single-nucleotide variants at gRNA-independent positions. The CBE system was used to simultaneously knockout five known antibiotic biosynthetic gene clusters to leave only an uncharacterized polyketide biosynthetic gene cluster in E681. The polyketide showed antimicrobial activities against gram-positive bacteria, but not gram-negative bacteria and fungi. Therefore, our findings suggested that the CBE system might serve as a powerful tool for multiplex genome editing and greatly accelerating the unraveling of hidden antibiotics in and species.
PubMed: 34122396
DOI: 10.3389/fmicb.2021.691839