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Journal of Food Protection Apr 2004The effectiveness of the strain CPA-2 of Pantoea agglomerans alone or in combination with a curing treatment at 33 degrees C for 65 h to control green mold was evaluated...
The effectiveness of the strain CPA-2 of Pantoea agglomerans alone or in combination with a curing treatment at 33 degrees C for 65 h to control green mold was evaluated on lemons stored at ambient temperature and in cold storage. An application of P. agglomerans at 2 x 10(8) CFU/ml effectively reduced green mold incidence on recently inoculated lemons stored at temperatures from 5 to 25 degrees C. Moreover, a 30-s immersion of lemons in a P. agglomerans suspension at 2 x 10(8) CFU/ml significantly reduced green mold incidence, even when delayed up to 15 h after inoculation with Penicillium digitatum at either 20 degrees C or while in cold storage. However, it failed to control established infections of P. digitatum of more than 24 h. Curing P. agglomerans-treated lemons at 33 degrees C for 65 h completely controlled 24-h-old infections on artificially inoculated lemons stored at 20 degrees C for 14 days and on naturally infected lemons stored at 10 degrees C for 3 weeks plus 7 additional days at 20 degrees C. When applied before curing, population growth of P. agglomerans in wounds was similar to that within wounds of control fruits at 20 degrees C. In contrast, when it was applied immediately after curing treatment, P. agglomerans populations within wounds did not increase.
Topics: Citrus; Colony Count, Microbial; Food Handling; Food Microbiology; Food Preservation; Pantoea; Penicillium; Pest Control, Biological; Temperature; Time Factors
PubMed: 15083731
DOI: 10.4315/0362-028x-67.4.781 -
Frontiers in Microbiology 2022Reduced agricultural production as well as issues like nutrient-depleted soils, eutrophication, and groundwater contamination have drawn attention to the use of...
Reduced agricultural production as well as issues like nutrient-depleted soils, eutrophication, and groundwater contamination have drawn attention to the use of endophyte-based bioformulations to restore soil fertility. CPHN2, a non-rhizobial nodule endophyte isolated from , exhibited a variety of plant growth-promoting traits. In this study, we used NextSeq500 technology to analyze whole-genome sequence information of this plant growth-promoting endophytic bacteria. The genome of CPHN2 has a length of 4,839,532 bp and a G + C content of 55.2%. The whole genome comprises three different genomic fractions, comprising one circular chromosome and two circular plasmids. A comparative analysis between CPHN2 and 10 genetically similar strains was performed using a bacterial pan-genome pipeline. All the predicted and annotated gene sequences for plant growth promotions (PGPs), such as phosphate solubilization, siderophore synthesis, nitrogen metabolism, and indole-3-acetic acid (IAA) of CPHN2, were identified. The whole-genome analysis of CPHN2 provides an insight into the mechanisms underlying PGP by endophytes and its potential applications as a biofertilizer.
PubMed: 36419432
DOI: 10.3389/fmicb.2022.998821 -
Journal of Microbiology, Immunology,... Jun 2013There are only three case reports of adult patients with spontaneous Pantoea agglomerans bacteremia in the English literature. The aim of this study was to investigate...
BACKGROUND/PURPOSE
There are only three case reports of adult patients with spontaneous Pantoea agglomerans bacteremia in the English literature. The aim of this study was to investigate clinical and microbiologic characteristics patients of P agglomerans bacteremia.
METHODS
We studied all adult patients with P agglomerans bacteremia at a medical center from 2000 to 2010. The isolates were identified using two commercial identification systems.
RESULTS
Of the 18 patients identified, 72% (n = 13) had active gastroesophageal disease treated with antacids. Two-thirds of patients had indwelling central lines and advanced cancers. None of the removed catheter tips yielded P agglomerans and line persistence was not associated with adverse outcomes. Initial disease severity was low, hypotension was uncommon and no patient died of bacteremia. Recurrence of bacteremia occurred in one patient with deep-seated infection. 16srRNA gene sequencing identified only half of the isolates as P agglomerans. The remaining nine isolates were Enterobacter species for six, Pantoea ananatis for two, and Exiguobacterium profundum for one. There were no significant differences between the characteristics of the subgroup molecularly identified as P agglomernas and the overall group characteristics. Eleven (61%) of the 18 isolates were susceptible to cefazolin, six (33%) susceptible to fosfomycin (MIC ≤ 64 mg/ml). Two isolates had colistin MICs ≥ 4 mg/ml.
CONCLUSION
Bacteremia caused by P agglomerans is associated with gastroesophageal reflux disease and receipt of antacids. 16srRNA gene sequencing should not be used as the sole basis for its identification and we have highlighted the need for another molecular-based technique to conclusively characterize P agglomerans.
Topics: Academic Medical Centers; Adult; Aged; Antacids; Bacteremia; Enterobacteriaceae Infections; Female; Gastroesophageal Reflux; Humans; Male; Middle Aged; Pantoea; Risk Factors; Taiwan
PubMed: 22841622
DOI: 10.1016/j.jmii.2012.05.005 -
Journal of Applied Microbiology 2002To reduce concentrations of protective and rehydrating media and to evaluate the effect of storage temperature, packaging and atmosphere conditions on the stability of...
AIMS
To reduce concentrations of protective and rehydrating media and to evaluate the effect of storage temperature, packaging and atmosphere conditions on the stability of freeze-dried Pantoea agglomerans cells. Efficacy against Penicillium digitatum of freeze-dried cells in orange fruits was also evaluated.
METHODS AND RESULTS
Several concentrations of protective and rehydration media were tested to reduce processing costs. Freeze-dried cells were packed in glass vials or plastic bags under vacuum or nitrogen conditions at 4 and 25 degrees C. After 1 and 3 months, efficacy of freeze-dried P. agglomerans against P. digitatum was tested.
CONCLUSIONS
The results indicate that it is possible to reduce the concentration of non-fat skimmed milk as a rehydration medium from 10% to 1%, maintaining viabilities of 100%. Moreover, freeze-dried cells could be stored in glass vials or in high barrier plastic bags at 4 degrees C for 3 months while maintaining high viabilities and efficacy against P. digitatum.
SIGNIFICANCE AND IMPACT OF THE STUDY
The major obstacle in the commercialization of biocontrol products is the development of a shelf-stable formulated product that retains biocontrol activity at a level similar to that of fresh cells. This study suggests that it is possible to maintain viability and efficacy of freeze-dried P. agglomerans cells for at least 3 months.
Topics: Atmosphere; Citrus; Food Handling; Food Packaging; Freeze Drying; Pantoea; Penicillium; Pest Control, Biological
PubMed: 11972691
DOI: 10.1046/j.1365-2672.2002.01596.x -
Scientific Reports Jun 2022The bacterium Pantoea sp. BCCS 001 GH produces an exopolysaccharide (EPS) named Pantoan through using sugar beet molasses (SBM) as an inexpensive and widely available...
The bacterium Pantoea sp. BCCS 001 GH produces an exopolysaccharide (EPS) named Pantoan through using sugar beet molasses (SBM) as an inexpensive and widely available carbon source. This study aims to investigate the kinetics and optimization of the Pantoan biosynthesis using Pantoea sp. BCCS 001 GH in submerged culture. During kinetics studies, the logistic model and Luedeking-Piret equation are precisely fit with the obtained experimental data. The response surface methodology (RSM)-central composite design (CCD) method is applied to evaluate the effects of four factors (SBM, peptone, NaHPO, and Triton X-100) on the concentration of Pantoan in batch culture of Pantoea sp. BCCS 001 GH. The experimental and predicted maximum Pantoan production yields are found 9.9 ± 0.5 and 10.30 g/L, respectively, and the best prediction factor concentrations are achieved at 31.5 g/L SBM, 2.73 g/L peptone, 3 g/L NaHPO and 0.32 g/L Triton X-100 after 48 h of submerged culture fermentation, at 30 °C. The functional groups and major monosaccharides (glucose and galactose) of a purified Pantoan are described and confirmed by HNMR and FTIR. The produced Pantoan is also characterized by thermogravimetric analysis and the rheological properties of the biopolymer are investigated. The present work guides the design and optimization of the Pantoea sp. BCCS 001 GH culture media, to be fine-tuned and applied to invaluable EPS, which can be applicable in food and biotechnology applications.
Topics: Culture Media; Fermentation; Kinetics; Molasses; Octoxynol; Pantoea; Peptones
PubMed: 35710936
DOI: 10.1038/s41598-022-14417-1 -
Environmental Microbiology Oct 2022The type VI secretion system (T6SS) is deployed by numerous Gram-negative bacteria to deliver toxic effectors into neighbouring cells. The genome of Pantoea agglomerans...
The type VI secretion system (T6SS) is deployed by numerous Gram-negative bacteria to deliver toxic effectors into neighbouring cells. The genome of Pantoea agglomerans pv. betae (Pab) phytopathogenic bacteria contains a gene cluster (T6SS1) predicted to encode a complete T6SS. Using secretion and competition assays, we found that T6SS1 in Pab is a functional antibacterial system that allows this pathogen to outcompete rival plant-associated bacteria found in its natural environment. Computational analysis of the T6SS1 gene cluster revealed that antibacterial effector and immunity proteins are encoded within three genomic islands that also harbour arrays of orphan immunity genes or toxin and immunity cassettes. Functional analyses indicated that VgrG, a specialized antibacterial effector, contains a C-terminal catalytically active glucosaminidase domain that is used to degrade prey peptidoglycan. Moreover, we confirmed that a bicistronic unit at the end of the T6SS1 cluster encodes a novel antibacterial T6SS effector and immunity pair. Together, these results demonstrate that Pab T6SS1 is an antibacterial system delivering a lysozyme-like effector to eliminate competitors, and indicate that this bacterium contains additional novel T6SS effectors.
Topics: Anti-Bacterial Agents; Bacterial Proteins; Hexosaminidases; Muramidase; Pantoea; Peptidoglycan; Type VI Secretion Systems
PubMed: 35706135
DOI: 10.1111/1462-2920.16100 -
PloS One 2018Pantoea agglomerans (P. agglomerans) is a Gram-negative bacterium that grows symbiotically with various edible plants, and the oral or sublingual administration of...
Oral administration of Pantoea agglomerans-derived lipopolysaccharide prevents development of atherosclerosis in high-fat diet-fed apoE-deficient mice via ameliorating hyperlipidemia, pro-inflammatory mediators and oxidative responses.
Pantoea agglomerans (P. agglomerans) is a Gram-negative bacterium that grows symbiotically with various edible plants, and the oral or sublingual administration of lipopolysaccharide derived from P. agglomerans (LPSp) have been suggested to contribute to prevention of immune-related diseases. Our previous study indicated that orally administered LPSp was shown to exhibit an LDL-lowering effect in hyperlipidemic volunteers; however, a preventive effect of LPSp on atherosclerosis is unclear. The present study attempted to evaluate the anti-atherosclerotic effect by LPSp in a mouse model of high-fat diet (HFD)-induced atherosclerosis. For 16 weeks, apoE-deficient mice were fed an HFD and received drinking water containing LPSp (0.3 or 1 mg/kg body weight/day). The results showed that the orally administered LPSp decreased body weight. A significant reduction in atherosclerotic plaque deposition was observed even with the lower dose of LPSp. The biochemical analyses showed that LPSp markedly improved glucose tolerance and reduced plasma LDL and oxidized LDL levels. In addition, LPSp significantly reduced the production of pro-inflammatory mediators including MCP-1 (in the plasma), TNF-α and IL-6 (in the colon), and decreased the oxidative burst activities in the peripheral blood sample. Taken together, these results suggest the possibility that oral administration of LPSp can effectively ameliorate HFD-induced hyperlipidemia and inflammatory/oxidative responses to prevent atherosclerosis and related metabolic disorders.
Topics: Administration, Oral; Animals; Apolipoproteins E; Body Weight; Chemokine CCL2; Diet, High-Fat; Hyperlipidemias; Interleukin-6; Intestines; Lipopolysaccharides; Lipoproteins, LDL; Male; Mice; Mice, Inbred BALB C; Mice, Knockout; Oxidative Stress; Pantoea; Plaque, Atherosclerotic; Tumor Necrosis Factor-alpha
PubMed: 29584779
DOI: 10.1371/journal.pone.0195008 -
Molecular Microbiology Sep 2006Pantoea agglomerans pv. gypsophilae (Pag) elicits galls on gypsophila and a hypersensitive response on beet, whereas P. agglomerans pv. betae (Pab) induces galls on both...
Pantoea agglomerans pv. gypsophilae (Pag) elicits galls on gypsophila and a hypersensitive response on beet, whereas P. agglomerans pv. betae (Pab) induces galls on both beet and gypsophila. The pathogenicity of both pathovars is dependent on the presence of a plasmid harbouring type III secretion system (TTSS) components and effectors. The HsvG TTSS effectors of Pag (HsvG-Pag) and Pab (HsvG-Pab) determine the host specificity of both pathovars on gypsophila. Here we describe a novel HsvG homologue, HsvB, which determines the host specificity of Pag and Pab on beet. HsvG requires two direct amino acid repeats for pathogenicity on gypsophila, whereas one repeat in HsvB is sufficient for pathogenicity on beet. Exchanging repeats between HsvG-Pag and HsvB-Pab resulted in a switch of host specificities. Transient expression of GFP-HsvG or GFP-HsvB fusions in gypsophila, beet or melon leaves showed that HsvG and HsvB were localized to the nuclei of host and non-host plants. A yeast one-hybrid assay revealed that a single repeat of HsvG or HsvB was sufficient to activate transcription. By employing random binding-site selection and gel-shift assay HsvG was demonstrated to be a double-stranded DNA-binding protein with an ACACC/aAA consensus binding site. These results suggest that HsvG and HsvB are host-specificity determinants and bear the potential to affect the host transcriptional machinery.
Topics: Bacterial Proteins; Base Sequence; Beta vulgaris; Caryophyllaceae; DNA-Binding Proteins; Electrophoretic Mobility Shift Assay; Green Fluorescent Proteins; Molecular Sequence Data; Pantoea; Plasmids; Recombinant Fusion Proteins; Repetitive Sequences, Amino Acid; Species Specificity; Trans-Activators; Transcription, Genetic; Two-Hybrid System Techniques; Virulence
PubMed: 16879413
DOI: 10.1111/j.1365-2958.2006.05301.x -
Journal of Applied Microbiology Mar 2016Assess the diversity of the culturable endophytic bacterial population associated with transgenic and nontransgenic soybean grown in field trial sites in Brazil and...
AIMS
Assess the diversity of the culturable endophytic bacterial population associated with transgenic and nontransgenic soybean grown in field trial sites in Brazil and characterize them phenotypically and genotypically focusing on characteristics related to plant growth promotion.
METHODS AND RESULTS
Endophytic bacteria were isolated from roots, stems and leaves of soybean cultivars (nontransgenic (C) and glyphosate-resistant (GR) transgenic soybean), including the isogenic BRS133 and BRS245RR. Significant differences were observed in bacterial densities in relation to genotype and tissue from which the isolates were obtained. The highest number of bacteria was observed in roots and in GR soybean. Based on characteristics related to plant growth promotion, 54 strains were identified by partial 16S rRNA sequence analysis, with most of the isolates belonging to the species Enterobacter ludwigii and Variovorax paradoxus. Among the isolates, 44·4% were able to either produce indoleacetic acid (IAA) or solubilize phosphates, and 9·2% (all from GR soybean) presented both plant growth-promoting activities.
CONCLUSIONS
The results from this study indicate that the abundance of endophytic bacterial communities of soybean differs between cultivars and in general it was higher in the transgenic cultivars than in nontransgenic cultivars. BRS 245 RR exhibited no significant difference in abundance compared to nontransgenic BRS133. This suggests that the impact of the management used in the GR soybean fields was comparable with the impacts of some enviromental factors. However, the bacterial endophytes associated to GR and nontransgenic soybean were different. The soybean-associated bacteria showing characteristics related to plant growth promotion were identified as belonging to the species Pantoea agglomerans and Variovorax paradoxus.
SIGNIFICANCE AND IMPACT OF THE STUDY
Our study demonstrated differences concerning compostion of culturable endophytic bacterial population in nontransgenic and transgenic soybean.
Topics: Bacteria; Brazil; Endophytes; Indoleacetic Acids; Pantoea; Plant Leaves; Plant Roots; RNA, Ribosomal, 16S; Glycine max
PubMed: 26744016
DOI: 10.1111/jam.13046 -
Journal of Bacteriology Aug 1998The fhuA genes of Salmonella paratyphi B, Salmonella typhimurium, and Pantoea agglomerans were sequenced and compared with the known fhuA sequence of Escherichia coli.... (Comparative Study)
Comparative Study
Identification of a new site for ferrichrome transport by comparison of the FhuA proteins of Escherichia coli, Salmonella paratyphi B, Salmonella typhimurium, and Pantoea agglomerans.
The fhuA genes of Salmonella paratyphi B, Salmonella typhimurium, and Pantoea agglomerans were sequenced and compared with the known fhuA sequence of Escherichia coli. The highly similar FhuA proteins displayed the largest difference in the predicted gating loop, which in E. coli controls the permeability of the FhuA channel and serves as the principal binding site for the phages T1, T5, and phi80. All the FhuA proteins contained the region in the gating loops required in E. coli for ferrichrome and albomycin transport. The three subdomains required for phage binding were contained in the gating loop of S. paratyphi B which is infected by the E. coli phages, whereas two of the subdomains were deleted in S. typhimurium and P. agglomerans which are resistant to the E. coli phages. Small deletions in a surface loop adjacent to the gating loop, residues 236 to 243 and 236 to 248, inactivated E. coli FhuA with regard to transport of ferrichrome and albomycin, but sensitivity to T1 and T5 was fully retained and sensitivity to phi80 and colicin M was reduced 10-fold. Full-size FhuA hybrid proteins of S. paratyphi B and S. typhimurium displayed S. paratyphi B FhuA activity when the hybrids contained two-thirds of either the N- or the C-terminal portions of S. paratyphi B and displayed S. typhimurium FhuA activity to phage ES18 when the hybrid contained two-thirds of the N-terminal region of the S. typhimurium FhuA. The central segment of the S. paratyphi B FhuA flanked on both sides by S. typhimurium FhuA regions conferred full sensitivity only to phage T5. The data support the essential role of the gating loop for the transport of ferrichrome and albomycin, identified an additional loop for ferrichrome and albomycin uptake, and suggest that several segments and their proper conformation, determined by the entire FhuA protein, contribute to the multiple FhuA activities.
Topics: Amino Acid Sequence; Bacterial Outer Membrane Proteins; Bacteriophages; Biological Transport; Cell Membrane Permeability; Cosmids; Enterobacteriaceae; Escherichia coli; Escherichia coli Proteins; Ferrichrome; Kinetics; Molecular Sequence Data; Plasmids; Receptors, Virus; Recombinant Proteins; Salmonella paratyphi A; Salmonella typhimurium; Sequence Alignment; Sequence Homology, Amino Acid
PubMed: 9683481
DOI: 10.1128/JB.180.15.3845-3852.1998