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Brazilian Oral Research May 2016This study assessed fluoride (F) and sodium trimetaphosphate (TMP) release into artificial saliva from varnishes containing 0%, 2.5%, and 5% NaF, supplemented or not...
This study assessed fluoride (F) and sodium trimetaphosphate (TMP) release into artificial saliva from varnishes containing 0%, 2.5%, and 5% NaF, supplemented or not with 5% TMP. The varnishes were applied on polyester sheets (n = 8/group), and F and TMP released into artificial saliva were measured for up to 24 hours. The amount of F and TMP released were directly related to NaF and TMP concentrations in the varnishes. The highest F release was seen for 5% NaF and 5% NaF + 5% TMP, whereas 5% TMP released the highest amount of TMP. However, the simultaneous addition of NaF and TMP to varnishes significantly reduced the amount of F and TMP released from the products.
Topics: Analysis of Variance; Cariostatic Agents; Fluorides; Polyphosphates; Reference Values; Saliva, Artificial; Sodium Fluoride; Statistics, Nonparametric; Time Factors
PubMed: 27223137
DOI: 10.1590/1807-3107BOR-2016.vol30.0064 -
Journal of Nuclear Medicine Technology Mar 2020The skeleton is the third most common site for metastasis overall, after the lungs and liver. Accurate diagnosis of osseous metastasis is critical for initial staging,... (Review)
Review
F-Sodium Fluoride PET: History, Technical Feasibility, Mechanism of Action, Normal Biodistribution, and Diagnostic Performance in Bone Metastasis Detection Compared with Other Imaging Modalities.
The skeleton is the third most common site for metastasis overall, after the lungs and liver. Accurate diagnosis of osseous metastasis is critical for initial staging, treatment planning, restaging, treatment monitoring, and survival prediction. Currently, Tc-methylene diphosphonate whole-body scanning is the cornerstone of imaging to detect osseous metastasis. Although F-sodium fluoride (F-NaF) was one of the oldest medical tracers for this purpose, it was replaced by other tracers because of their better physical properties, until recently. Continued development of PET scanners has opened a new era for F-NaF, and given its higher sensitivity, there have been increasing applications in imaging. In this review, we will discuss the history, technical aspects, radiobiology, and biodistribution of this tracer. Finally, we compare the accuracy of F-NaF PET with other conventional imaging methods for detection of osseous metastasis.
Topics: Bone Neoplasms; Bone and Bones; Diphosphonates; Feasibility Studies; Fluorine Radioisotopes; Humans; Magnetic Resonance Imaging; Male; Multimodal Imaging; Positron-Emission Tomography; Radiopharmaceuticals; Sodium Fluoride; Technetium; Tissue Distribution; Tomography, X-Ray Computed; Whole Body Imaging
PubMed: 31811067
DOI: 10.2967/jnmt.119.234336 -
Naunyn-Schmiedeberg's Archives of... Apr 2024Carbachol, an agonist at muscarinic receptors, exerts a negative inotropic effect in human atrium. Carbachol can activate protein phosphatases (PP1 or PP2A). We...
Carbachol, an agonist at muscarinic receptors, exerts a negative inotropic effect in human atrium. Carbachol can activate protein phosphatases (PP1 or PP2A). We hypothesized that cantharidin or sodium fluoride, inhibitors of PP1 and PP2A, may attenuate a negative inotropic effect of carbachol. During bypass-surgery trabeculae carneae of human atrial preparations (HAP) were obtained. These trabeculae were mounted in organ baths and electrically stimulated (1 Hz). Force of contraction was measured under isometric conditions. For comparison, we studied isolated electrically stimulated left atrial preparations (LA) from mice. Cantharidin (100 µM) and sodium fluoride (3 mM) increased force of contraction in LA (n = 5-8, p < 0.05) by 113% ± 24.5% and by 100% ± 38.2% and in HAP (n = 13-15, p < 0.05) by 625% ± 169% and by 196% ± 23.5%, respectively. Carbachol (1 µM) alone exerted a rapid transient maximum negative inotropic effect in LA (n = 6) and HAP (n = 14) to 46.9% ± 3.63% and 19.4% ± 3.74%, respectively (p < 0.05). These negative inotropic effects were smaller in LA (n = 4-6) and HAP (n = 9-12) pretreated with 100 µM cantharidin and amounted to 58.0% ± 2.27% and 59.2% ± 6.19% or 3 mM sodium fluoride to 63.7% ± 9.84% and 46.3% ± 5.69%, (p < 0.05). We suggest that carbachol, at least in part, exerts a negative inotropic effect in the human atrium by stimulating the enzymatic activity of PP1 and/or PP2A.
Topics: Humans; Mice; Animals; Carbachol; Cantharidin; Sodium Fluoride; Myocardial Contraction; Heart Atria
PubMed: 37801145
DOI: 10.1007/s00210-023-02747-4 -
Scientific Reports Mar 2022Among the available methods of enamel strengthening, fluoride varnish (FV) treatment has relatively better results. On the other hand, cold plasma technology has shown...
Among the available methods of enamel strengthening, fluoride varnish (FV) treatment has relatively better results. On the other hand, cold plasma technology has shown promising capacities in sterilizing the environment, surface modification, and improving adhesion. Accordingly, this study aimed to increase the adhesion of FV to the enamel surface to prolong the enamel interaction with FV with subsequently increased fluoride uptake by enamel. Emphasizing that the change in adhesion is evidence-based and has not been explicitly measured. For this purpose, we randomly divided twenty bovine teeth into two groups A (consisting of four teeth) and B (composed of four subgroups, each containing four teeth). Samples of group A and one specimen of each subset B investigated the effect of using Helium-DBD (He-DBDJ), Argon (ArJ), and Air-DBD jet on the enamel surface. Other B specimens are devoted to studying the release of FV fluoride ions from processed enamel. Two diagnostic techniques, scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS), have been utilized to examine the samples' surface morphology and chemical analysis, respectively. Finally, the release of fluoride ions into distilled water was measured by an ion-selective electrode (ISE). SEM images showed that ArJ and Air-DBD significantly damaged enamel hexagonal structures, whereas, in the case of He-DBDJ, the hexagonal structures have only altered from convex to concave. EDX indicated an increase in calcium to phosphorus ratio and the amount of fluoride and sodium uptake on the enamel surface layer in the group processed with He-DBDJ plasma. The latter helps restore the damaged parts of the enamel. Analysis of fluoride released from the FV did not show a significant change owing to plasma processing (P ≤ 0.112). The combination of cold plasma and fluoride varnish treatment on the enamel surface might be considered as a more promising approach to increasing enamel resistance to tooth decay.
Topics: Animals; Cattle; Dental Enamel; Fluorides; Fluorides, Topical; Plasma Gases; Sodium Fluoride; Spectrometry, X-Ray Emission
PubMed: 35304509
DOI: 10.1038/s41598-022-08069-4 -
Journal of the Indian Society of... 2022Caries management in the modern era has shifted away from the traditional surgical method toward a medical paradigm, which often includes fluoride therapy. Fluoride used... (Randomized Controlled Trial)
Randomized Controlled Trial
BACKGROUND
Caries management in the modern era has shifted away from the traditional surgical method toward a medical paradigm, which often includes fluoride therapy. Fluoride used in various forms has been proven to be effective in dental caries prevention. Silver diamine fluoride (SDF) and sodium fluoride (NaF) varnish are effective in arresting caries in primary molars.
AIM
This study aimed to evaluate the effectiveness of 38% SDF and 5% NaF varnish in arresting caries in primary molars.
SETTINGS AND DESIGN
This study was a split-mouth randomized controlled trial.
MATERIALS AND METHODS
The randomized controlled trial comprised 34 children aged 6-9 years with teeth having caries in both right and left primary molars without pulpal involvement. Teeth were randomly allocated into two groups. In Group 1 (n = 34), 38% SDF + potassium iodide was applied and in Group 2 (n = 34), 5% NaF varnish was applied. The second application was done after 6 months in both groups. Children were recalled at 6- and 12-month intervals and evaluated for caries arrest.
STATISTICAL ANALYSIS
Chi-square test was used to analyze the data.
RESULTS
Caries arresting potential was found to be higher in the SDF group compared to the NaF varnish group at both 6-month (SDF - 82% and NaF varnish - 45%) and 12-month intervals (SDF - 77% and NaF varnish - 42%) and the difference was found to be statistically significant (P = 0.002 and 0.004, respectively).
CONCLUSION
SDF was more effective in arresting dental caries in primary molars compared to 5% NaF varnish.
Topics: Child; Humans; Sodium Fluoride; Dental Caries; Fluorides, Topical; Fluorides; Dental Caries Susceptibility; Molar; Sodium
PubMed: 36861553
DOI: 10.4103/jisppd.jisppd_239_22 -
International Journal of Molecular... Apr 2018This study investigated the effect of 25% silver nitrate (AgNO₃) and 5% sodium fluoride (NaF) varnish with functionalized tri-calcium phosphate (fTCP) on a () biofilm...
This study investigated the effect of 25% silver nitrate (AgNO₃) and 5% sodium fluoride (NaF) varnish with functionalized tri-calcium phosphate (fTCP) on a () biofilm and dentine caries lesion. Demineralised dentine specimens were treated with 25% AgNO₃ and 5% NaF + fTCP (Group 1), 25% AgNO₃ and 5% NaF (Group 2), 25% AgNO₃ (Group 3), or water (Group 4). The specimens were subjected to a biofilm challenge after treatment. The biofilm was then studied via scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM), and colony forming units (CFU). The specimens were assessed by micro-computed tomography, X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). SEM and CLSM revealed less biofilm in Groups 1 to 3. The log CFU of Groups 1 to 4 were 4.5 ± 0.7, 4.4 ± 0.9, 4.4 ± 0.9, and 6.7 ± 1.0, respectively (Groups 1, 2, 3 < 4, < 0.001). The lesion depths of Groups 1 to 4 were 212.6 ± 20.1 µm, 280.8 ± 51.6 µm, 402.5 ± 61.7 µm, and 497.4 ± 67.2 µm, respectively (Groups 1 < 2 < 3 < 4, < 0.001). XRD demonstrated silver chloride formation in Groups 1, 2, and 3. FTIR found the amide I: HPO₄ values of the four groups were 0.22 ± 0.05, 0.25 ± 0.05, 0.41 ± 0.12, and 0.64 ± 0.14, respectively (Groups 1, 2 < 3 < 4; < 0.001). In conclusion, this study revealed that AgNO₃ and NaF + fTCP reduced the damage of dentine caries by cariogenic biofilm.
Topics: Anti-Bacterial Agents; Biofilms; Calcium Phosphates; Dentin; Humans; Silver Nitrate; Sodium Fluoride; Streptococcus mutans
PubMed: 29693627
DOI: 10.3390/ijms19051288 -
Journal of Nuclear Cardiology :... Jun 2020
Topics: Aortic Valve; Multimodal Imaging; Positron-Emission Tomography; Sodium; Sodium Fluoride
PubMed: 30693427
DOI: 10.1007/s12350-019-01607-0 -
BMC Microbiology Aug 2015Cetylpyridinium chloride (CPC) and sodium fluoride augment oral hygiene by inactivating bacteria and inhibiting enamel demineralisation, respectively. However, there are...
BACKGROUND
Cetylpyridinium chloride (CPC) and sodium fluoride augment oral hygiene by inactivating bacteria and inhibiting enamel demineralisation, respectively. However, there are few reports in the literature documenting the antibacterial efficacy of their combined use in mouthrinses. We have used six experimental systems to compare the antibacterial effects of mouthrinses containing 0.075% CPC (test rinse, TR) or 0.075% CPC with sodium fluoride (test fluoride rinse, TFR).
RESULTS
Effects against planktonic bacteria were determined using viable counting (for Streptococcus mutans and salivary bacteria), a redox dye (for Actinomyces viscosus and salivary bacteria) and viable counting (for ex vivo oral rinses). Effects against saliva-derived biofilms were quantified using confocal microscopy and differential viable counting. Inhibition of biofilm formation was evaluated by pre-treating hydroxyapatite coupons with mouthrinses prior to inoculation. Otherwise-identical controls without CPC (control rinse and control fluoride rinse, CR and CFR, respectively), were included throughout. Compared to the controls, TFR and TR demonstrated significant antimicrobial effects in the redox assays, by viable counts (>3 log reductions) and in oral rinse samples (>1.25 log reductions, p < 0.05). TFR and TR also significantly reduced the viability of oral biofilms. Pre-treatment of hydroxyapatite with TFR and TR significantly inhibited biofilm formation (>3 log difference, p < 0.05). Overall, there were no consistent differences in the activities of TR and TFR.
CONCLUSIONS
Sodium fluoride did not influence the antibacterial and anti-biofilm potency of CPC-containing formulations, supporting the combined use of CPC and sodium fluoride in mouthrinses to control oral bacteria and protect tooth enamel.
Topics: Adult; Anti-Infective Agents, Local; Biofilms; Cetylpyridinium; Female; Humans; Male; Microbial Viability; Mouthwashes; Sodium Fluoride; Treatment Outcome; Young Adult
PubMed: 26293609
DOI: 10.1186/s12866-015-0501-x -
Archives of Oral Biology Feb 2020The aim of this study was to evaluate the morphological and chemical effect of in-office and at-home desensitising agents containing sodium fluoride (NaF) on eroded root...
OBJECTIVE
The aim of this study was to evaluate the morphological and chemical effect of in-office and at-home desensitising agents containing sodium fluoride (NaF) on eroded root dentine in vitro.
METHODS
Fifty bovine dentine samples were pre-eroded and randomised into five groups (n = 10): G1 (Control) - milli-Q water; G2 - fluoride varnish containing NaF 22,500 ppm; G3 - desensitising cream containing NaF 9,000 ppm associated with 20% nanohydroxyapatite; G4 - toothpaste with NaF 5,000 ppm associated to tricalcium phosphate; G5 - toothpaste containing NaF 900 ppm and casein phosphopeptide-amorphous calcium phosphate fluoride (CPP-ACPF). The specimens were submitted to erosive challenge for three days. The analyses were performed using non-contact profilometry for volumetric (Sa) and linear roughness (Ra) followed by scanning electron microscopy (SEM) and Energy Dispersive X-ray Spectrometry (EDS). The data were analysed by Kruskal-Wallis and Wilcoxon tests (α = 0.05).
RESULTS
There was a significant reduction of Ra and Sa for the eroded samples from the G2 and G5 (p < 0.05) after an erosive challenge. The dentine surface topography pattern showed partially or totally occluded dentinal tubules after treatments, except in the control group. The control, G4 and G3 groups showed a reduction in the dentine inorganic content percentage of Ca (Calcium) and P (Phosphorus) minerals.
CONCLUSION
The fluoride varnish and CPP-ACPF toothpaste were able to prevent morphological changes and were the only materials that showed the Ca and P content increased after treatment. These materials may be promising alternatives in the clinical control of dentin erosion.
Topics: Animals; Cattle; Dentin; Fluorides; Sodium Fluoride; Tooth Erosion; Tooth Root; Toothpastes
PubMed: 31805483
DOI: 10.1016/j.archoralbio.2019.104619 -
Scientific Reports Jan 2021Nephrocalcinosis is present in up to 43% of kidney allograft biopsies at one-year after transplantation and is associated with inferior graft function and poor graft...
Nephrocalcinosis is present in up to 43% of kidney allograft biopsies at one-year after transplantation and is associated with inferior graft function and poor graft survival. We studied [F]-sodium fluoride ([F]-NaF) imaging of microcalcifications in donor kidneys (n = 7) and explanted kidney allografts (n = 13). Three µm paraffin-embedded serial sections were used for histological evaluation of calcification (Alizarin Red; Von Kossa staining) and ex-vivo [F]-NaF autoradiography. The images were fused to evaluate if microcalcification areas corresponded with [F]-NaF uptake areas. Based on histological analyses, tubulointerstitial and glomerular microcalcifications were present in 19/20 and 7/20 samples, respectively. Using autoradiography, [F]-NaF uptake was found in 19/20 samples, with significantly more tracer activity in kidney allograft compared to deceased donor kidney samples (p = 0.019). Alizarin Red staining of active microcalcifications demonstrated good correlation (Spearman's rho of 0.81, p < 0.001) and Von Kossa staining of consolidated calcifications demonstrated significant but weak correlation (0.62, p = 0.003) with [F]-NaF activity. This correlation between ex-vivo [F]-NaF uptake and histology-proven microcalcifications, is the first step towards an imaging method to identify microcalcifications in active nephrocalcinosis. This may lead to better understanding of the etiology of microcalcifications and its impact on kidney transplant function.
Topics: Aged; Allografts; Autoradiography; Female; Fluorine Radioisotopes; Humans; Kidney; Kidney Transplantation; Male; Middle Aged; Nephrocalcinosis; Positron Emission Tomography Computed Tomography; Sodium Fluoride; Tissue Donors
PubMed: 33469037
DOI: 10.1038/s41598-021-81144-4